FastQCFastQC Report
Sat 18 Jun 2016
SRR3554052_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554052_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1138194
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCTGC18340.16113246072286447RNA PCR Primer, Index 40 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCTGCT16420.1442636316831753Illumina PCR Primer Index 8 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15770.13855283018536382No Hit
CCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCTGC12560.11035025663463345Illumina PCR Primer Index 8 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGACG406.8157533E-945.0000041
TTACGCT302.1654832E-644.99999641
CGGTCTA603.6379788E-1241.24999631
TTACGCG501.0822987E-940.51
CGGATAT451.9283107E-840.06
CGTTTTT14350.039.8257831
GCGATAC800.039.3750049
GCTAGCG1100.038.8636361
TCGTTAC356.248234E-638.5714338
CATACGA1300.038.0769218
TACGAAT950.037.89473712
CGAATAT950.037.89473714
GCAACGA1200.037.49999611
CGGTTGA301.1399149E-437.49999610
TACGATT301.1399149E-437.49999611
TCGTAAG301.1399149E-437.4999961
GTACGAT301.1399149E-437.49999610
ACGGTTG301.1399149E-437.49999611
GTATTCG552.748493E-936.818181
ATTAGCG1100.036.818181
Produced by FastQC (version 0.11.5)