##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554052_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1138194
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.03196203810598	33.0	31.0	34.0	30.0	34.0
2	32.136885276148	33.0	31.0	34.0	30.0	34.0
3	32.16628272508905	34.0	31.0	34.0	30.0	34.0
4	35.73409453924375	37.0	35.0	37.0	33.0	37.0
5	35.61516753734425	37.0	35.0	37.0	33.0	37.0
6	35.61056111699763	37.0	35.0	37.0	33.0	37.0
7	35.871166953963915	37.0	35.0	37.0	35.0	37.0
8	35.76570162907202	37.0	35.0	37.0	35.0	37.0
9	37.42504353387911	39.0	37.0	39.0	35.0	39.0
10	36.94609530536974	39.0	37.0	39.0	33.0	39.0
11	36.94007436342135	39.0	37.0	39.0	33.0	39.0
12	36.90173994942866	39.0	37.0	39.0	33.0	39.0
13	36.92258173914113	39.0	37.0	39.0	33.0	39.0
14	38.081896407818	40.0	37.0	41.0	33.0	41.0
15	38.19202086814726	40.0	37.0	41.0	33.0	41.0
16	38.22784165089607	40.0	37.0	41.0	33.0	41.0
17	38.200497454739704	40.0	37.0	41.0	33.0	41.0
18	38.152502121782405	40.0	37.0	41.0	33.0	41.0
19	38.11553566439465	40.0	37.0	41.0	34.0	41.0
20	38.066893692990824	40.0	37.0	41.0	34.0	41.0
21	37.95798607267302	40.0	37.0	41.0	33.0	41.0
22	37.94265652428321	40.0	37.0	41.0	33.0	41.0
23	37.899320326763274	40.0	37.0	41.0	33.0	41.0
24	37.87957501093838	40.0	37.0	41.0	33.0	41.0
25	37.784045602067835	40.0	36.0	41.0	33.0	41.0
26	37.74908758963762	40.0	36.0	41.0	33.0	41.0
27	37.694470362697395	40.0	36.0	41.0	33.0	41.0
28	37.58088339949077	40.0	36.0	41.0	33.0	41.0
29	37.5740515237297	40.0	36.0	41.0	33.0	41.0
30	37.43945847544443	40.0	36.0	41.0	32.0	41.0
31	37.354978149594885	39.0	36.0	41.0	32.0	41.0
32	37.27724184102183	39.0	36.0	41.0	32.0	41.0
33	37.17995526245965	39.0	35.0	41.0	31.0	41.0
34	37.10159428006122	39.0	35.0	41.0	31.0	41.0
35	37.046948059821084	39.0	35.0	41.0	31.0	41.0
36	36.94125342428443	39.0	35.0	41.0	31.0	41.0
37	36.91796214002182	39.0	35.0	41.0	31.0	41.0
38	36.82661128067799	39.0	35.0	41.0	31.0	41.0
39	36.76964647502974	39.0	35.0	41.0	31.0	41.0
40	36.649961254408296	39.0	35.0	41.0	30.0	41.0
41	36.52646473272571	39.0	35.0	41.0	30.0	41.0
42	36.44215045941202	39.0	35.0	41.0	30.0	41.0
43	36.3542076306851	39.0	35.0	40.0	30.0	41.0
44	36.23608189816499	39.0	35.0	40.0	30.0	41.0
45	36.16010451645326	38.0	35.0	40.0	29.0	41.0
46	36.01336239692003	38.0	35.0	40.0	29.0	41.0
47	35.9015510536868	38.0	35.0	40.0	29.0	41.0
48	35.813776034665445	38.0	35.0	40.0	29.0	41.0
49	35.72667576880567	38.0	34.0	40.0	28.0	41.0
50	35.60750188456449	38.0	34.0	40.0	28.0	41.0
51	34.04390552050002	36.0	32.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	7.0
10	4.0
11	3.0
12	12.0
13	18.0
14	31.0
15	38.0
16	92.0
17	148.0
18	301.0
19	554.0
20	942.0
21	1465.0
22	2205.0
23	3166.0
24	4615.0
25	6349.0
26	8630.0
27	10591.0
28	12586.0
29	15763.0
30	19826.0
31	25223.0
32	32634.0
33	44326.0
34	70156.0
35	87858.0
36	96731.0
37	141195.0
38	230792.0
39	321832.0
40	99.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.02861023691919	19.135050790989936	24.470432984183716	12.365905987907158
2	30.754511093890848	24.893208011990925	26.294726558038434	18.05755433607979
3	33.26585801717458	24.156953911196158	26.000488493174274	16.57669957845499
4	27.88707373259743	27.12577996369687	25.579470635058698	19.407675668647
5	25.14562543819419	31.17377178231479	24.72987908915352	18.9507236903375
6	24.098528018949317	36.91462088185318	24.073839784781857	14.913011314415645
7	83.68195580015357	5.615387183555703	6.770199104897759	3.9324579113929614
8	85.95529408870544	4.276863170953282	5.734435430163926	4.033407310177351
9	79.43751241001095	7.307365879630362	8.250966004037975	5.004155706320715
10	38.259646422314646	28.52352059490737	17.098227542932047	16.118605439845933
11	29.57694382504213	27.44874775301926	23.273800424180763	19.700507997757853
12	25.8696672096321	24.49046471866835	29.034066248811712	20.605801822887837
13	24.142984412147666	25.80596980831036	30.14178602241797	19.909259757124005
14	22.086832297481802	28.516491916140836	28.55005385725105	20.846621929126318
15	21.332215773409455	27.41237434040243	31.03434036728361	20.22106951890451
16	23.512160492850956	25.82749513703288	29.97608492049686	20.68425944961931
17	23.627870117045074	26.060320121174424	27.26582638812013	23.045983373660377
18	24.714855288290043	25.620676264327525	27.941106700615187	21.723361746767246
19	24.429578788853217	28.095474058025253	26.093179194407984	21.381767958713542
20	25.90129626408152	26.683236776858777	26.463414848435328	20.952052110624376
21	24.780397717788006	27.618051052808223	27.086507221088844	20.515044008314927
22	23.585346610507525	25.687360854125046	26.81616666403091	23.911125871336523
23	22.76079473270813	27.72418410218293	26.465259876611547	23.049761288497393
24	22.801385352584884	27.079302825353146	27.838400132139164	22.280911689922807
25	22.993092565942185	27.755373864209442	26.46912564993314	22.782407919915233
26	22.196655403208943	29.245541621199898	26.20897667708668	22.348826298504473
27	23.42491701766131	27.97317504748751	26.538533852752693	22.063374082098484
28	22.147542510327764	28.450949486642873	27.530895436103158	21.8706125669262
29	23.856565752411278	27.432669650340802	26.431346501563002	22.279418095684917
30	24.39083319715268	27.70889672586571	25.67593925112942	22.224330825852185
31	23.995382158050386	28.9788911204944	24.772314737206486	22.253411984248732
32	24.686301280800986	28.894546975295953	25.16346071056428	21.25569103333878
33	23.928346134314538	28.95850795207144	23.96682815056133	23.146317763052696
34	22.9055855152988	29.271020581728596	25.648175970001596	22.175217932971005
35	24.6756704041666	27.68772283108152	25.012168400114565	22.62443836463731
36	23.85841078058749	30.283501758048274	24.064966077839102	21.79312138352513
37	23.298488658348226	30.4313675875993	25.092822488960582	21.17732126509189
38	23.525514982507374	30.095923893466313	24.740685682757068	21.637875441269237
39	23.81123077436711	27.797545936808664	25.259929326635	23.131293962189222
40	24.17101126872923	27.209772674956994	26.713899387977797	21.905316668335978
41	22.057223988177764	27.48318827897529	26.324159150373312	24.13542858247364
42	23.221173191916318	27.883295817760416	25.48836138654746	23.407169603775806
43	23.209751588920692	26.735776150638646	26.932403439132518	23.122068821308144
44	23.402073811670064	27.261169888437298	26.066909507518048	23.269846792374587
45	23.49810313531788	26.948832975749298	25.64975742272407	23.903306466208747
46	23.83732474428788	28.20116781497706	24.967799865400803	22.99370757533426
47	22.847159623051958	27.89173023228026	26.560674190867285	22.700435953800493
48	22.579981971438965	27.346304759996976	26.466226319941942	23.607486948622117
49	23.14447273487648	26.915886044031158	26.894448573793216	23.045192647299142
50	22.084987269305582	26.53387735306986	27.243334616067212	24.137800761557344
51	22.20403551591381	26.43125864307842	25.753342576045913	25.611363264961863
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	681.0
1	924.0
2	1167.0
3	2172.5
4	3178.0
5	2371.5
6	1565.0
7	1515.5
8	1466.0
9	1529.0
10	1592.0
11	1648.5
12	1705.0
13	1752.0
14	1799.0
15	1874.0
16	1949.0
17	2075.5
18	2202.0
19	2403.0
20	2604.0
21	3082.0
22	3560.0
23	3830.5
24	4101.0
25	5324.5
26	7818.5
27	9089.0
28	11327.0
29	13565.0
30	16262.0
31	18959.0
32	21741.5
33	24524.0
34	27033.5
35	29543.0
36	33313.5
37	37084.0
38	39466.0
39	41848.0
40	47259.0
41	52670.0
42	57932.5
43	63195.0
44	70090.0
45	76985.0
46	85575.0
47	94165.0
48	103115.0
49	112065.0
50	109205.0
51	106345.0
52	97985.5
53	89626.0
54	79443.0
55	69260.0
56	63133.5
57	57007.0
58	53199.0
59	49391.0
60	45811.5
61	42232.0
62	38010.5
63	33789.0
64	30319.5
65	26850.0
66	22076.5
67	17303.0
68	15119.5
69	12936.0
70	10913.0
71	8890.0
72	7384.5
73	5879.0
74	5351.0
75	3889.0
76	2955.0
77	2114.0
78	1273.0
79	1095.0
80	917.0
81	721.5
82	526.0
83	357.0
84	188.0
85	124.0
86	60.0
87	53.5
88	47.0
89	41.5
90	36.0
91	24.5
92	13.0
93	10.5
94	8.0
95	17.5
96	27.0
97	15.0
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1138194.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.3003503599787
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.2117534896269	22.183417812040883
2	9.73582561238546	5.8999785419786726
3	3.609203307669816	3.2808037422836827
4	1.9191669736391521	2.326057268022653
5	1.288516217620136	1.9521246419202343
6	0.9872670227895758	1.7948722013626333
7	0.7722733159928936	1.6380106433773054
8	0.6453226210930494	1.5642801211471342
9	0.5608606976289683	1.5294848077169834
>10	6.7754694545443055	46.00523380318567
>50	0.4411275659390764	8.181725090490831
>100	0.049724296738844674	2.6311985648714673
>500	0.0020354975269289487	0.3673263508584648
>1k	0.0014539268049492489	0.645486410743371
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCTGC	1834	0.16113246072286447	RNA PCR Primer, Index 40 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCTGCT	1642	0.1442636316831753	Illumina PCR Primer Index 8 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1577	0.13855283018536382	No Hit
CCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCTGC	1256	0.11035025663463345	Illumina PCR Primer Index 8 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.785848458171454E-5	0.0	0.0	0.1534887725642553	0.0
2	8.785848458171454E-5	0.0	0.0	0.569674414027837	0.0
3	8.785848458171454E-5	0.0	0.0	0.7917806630504114	0.0
4	8.785848458171454E-5	0.0	0.0	1.0865458788220637	0.0
5	8.785848458171454E-5	0.0	0.0	1.8670806558460158	0.0
6	8.785848458171454E-5	0.0	0.0	2.658861318896427	0.0
7	8.785848458171454E-5	0.0	0.0	3.2722013997613764	0.0
8	8.785848458171454E-5	0.0	0.0	4.317893083252943	0.0
9	8.785848458171454E-5	0.0	0.0	4.753495449809083	0.0
10	1.7571696916342908E-4	0.0	0.0	5.613981447802396	0.0
11	1.7571696916342908E-4	0.0	0.0	6.700878760562786	0.0
12	1.7571696916342908E-4	0.0	0.0	7.591060926344718	0.0
13	1.7571696916342908E-4	0.0	0.0	7.982382616671675	0.0
14	2.635754537451436E-4	0.0	0.0	8.167851877623674	0.0
15	2.635754537451436E-4	0.0	0.0	8.43002159561551	0.0
16	2.635754537451436E-4	0.0	0.0	8.979488558189553	0.0
17	2.635754537451436E-4	0.0	0.0	9.66496045489609	0.0
18	2.635754537451436E-4	0.0	0.0	10.400863121752531	0.0
19	2.635754537451436E-4	0.0	0.0	10.847535657365967	0.0
20	2.635754537451436E-4	0.0	0.0	11.259943383992535	0.0
21	2.635754537451436E-4	0.0	0.0	11.794913696610596	0.0
22	2.635754537451436E-4	0.0	0.0	12.350355036136195	0.0
23	2.635754537451436E-4	0.0	0.0	12.926179544084752	0.0
24	2.635754537451436E-4	0.0	0.0	13.377772154834764	0.0
25	5.271509074902872E-4	0.0	0.0	13.787631985408463	0.0
26	5.271509074902872E-4	0.0	0.0	14.174121459083425	0.0
27	5.271509074902872E-4	0.0	0.0	14.573350413022736	0.0
28	5.271509074902872E-4	0.0	0.0	14.989975346909226	0.0
29	5.271509074902872E-4	0.0	0.0	15.42215123256668	0.0
30	5.271509074902872E-4	0.0	0.0	15.91767308560755	0.0
31	5.271509074902872E-4	0.0	0.0	16.37269217725625	0.0
32	5.271509074902872E-4	0.0	0.0	16.827799127389532	0.0
33	5.271509074902872E-4	0.0	0.0	17.2464448064214	0.0
34	5.271509074902872E-4	0.0	0.0	17.66658407969116	0.0
35	5.271509074902872E-4	0.0	0.0	18.098935682317776	0.0
36	5.271509074902872E-4	0.0	0.0	18.530320841613996	0.0
37	7.028678766537163E-4	0.0	0.0	18.96038812364149	0.0
38	7.028678766537163E-4	0.0	0.0	19.393179018691015	0.0
39	7.028678766537163E-4	0.0	0.0	19.82851780979341	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGACG	40	6.8157533E-9	45.000004	1
TTACGCT	30	2.1654832E-6	44.999996	41
CGGTCTA	60	3.6379788E-12	41.249996	31
TTACGCG	50	1.0822987E-9	40.5	1
CGGATAT	45	1.9283107E-8	40.0	6
CGTTTTT	1435	0.0	39.825783	1
GCGATAC	80	0.0	39.375004	9
GCTAGCG	110	0.0	38.863636	1
TCGTTAC	35	6.248234E-6	38.57143	38
CATACGA	130	0.0	38.07692	18
TACGAAT	95	0.0	37.894737	12
CGAATAT	95	0.0	37.894737	14
GCAACGA	120	0.0	37.499996	11
CGGTTGA	30	1.1399149E-4	37.499996	10
TACGATT	30	1.1399149E-4	37.499996	11
TCGTAAG	30	1.1399149E-4	37.499996	1
GTACGAT	30	1.1399149E-4	37.499996	10
ACGGTTG	30	1.1399149E-4	37.499996	11
GTATTCG	55	2.748493E-9	36.81818	1
ATTAGCG	110	0.0	36.81818	1
>>END_MODULE