FastQCFastQC Report
Sat 18 Jun 2016
SRR3554048_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554048_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences804993
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCG26190.32534444398895396No Hit
GAATCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTTC23080.2867105676695325No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGC22000.2732943019380293No Hit
GCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTTCTGC13510.1678275463264898No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT10930.1357775781901209No Hit
CCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTTCTGC10580.13142971429565226No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT9140.11354135998698126No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT8750.10869659736171618No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACG207.0320244E-445.0000041
CAATCGA207.0320244E-445.00000428
CGTCACA207.0320244E-445.00000423
GCGAATG351.2113196E-745.01
ACCGAAC253.889761E-545.010
TAGTTCG253.889761E-545.040
TCGACAC253.889761E-545.034
AGATCGG501.0804797E-940.52
GCGTACG501.0804797E-940.51
TTTAACG403.4564982E-739.37500439
TTACGGG1600.039.3750043
AATAGCG356.2459585E-638.5714261
AAGGGAT5550.038.513515
GCTACGA1350.038.33333610
TTGAGCG659.094947E-1238.0769271
TATGCGG900.037.52
GCATCCG301.139618E-437.49999628
ACGGGCT2500.036.95
GCGATAT552.746674E-936.818189
GTACGAG552.746674E-936.818181