##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554048_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	804993
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.046161892090986	33.0	31.0	34.0	30.0	34.0
2	32.17878540558738	34.0	31.0	34.0	30.0	34.0
3	32.18839542704098	34.0	31.0	34.0	30.0	34.0
4	35.765000440997625	37.0	35.0	37.0	35.0	37.0
5	35.64260310338102	37.0	35.0	37.0	33.0	37.0
6	35.61529354913645	37.0	35.0	37.0	33.0	37.0
7	35.82406679312739	37.0	35.0	37.0	35.0	37.0
8	35.648242903975564	37.0	35.0	37.0	35.0	37.0
9	37.3522800819386	39.0	37.0	39.0	35.0	39.0
10	36.94902191696077	39.0	37.0	39.0	33.0	39.0
11	36.89235682794757	39.0	37.0	39.0	33.0	39.0
12	36.71183848803654	39.0	35.0	39.0	33.0	39.0
13	36.63181667418226	39.0	35.0	39.0	33.0	39.0
14	37.69409920334711	40.0	36.0	41.0	33.0	41.0
15	37.82976994831011	40.0	36.0	41.0	33.0	41.0
16	37.939887676041906	40.0	36.0	41.0	33.0	41.0
17	37.87815670446824	40.0	36.0	41.0	33.0	41.0
18	37.85014279627276	40.0	36.0	41.0	33.0	41.0
19	37.81547292957827	40.0	36.0	41.0	33.0	41.0
20	37.718749107135096	40.0	36.0	41.0	33.0	41.0
21	37.62360293816219	40.0	36.0	41.0	33.0	41.0
22	37.622400443233666	39.0	35.0	41.0	33.0	41.0
23	37.54122209758346	39.0	35.0	41.0	33.0	41.0
24	37.50502178279811	39.0	35.0	41.0	33.0	41.0
25	37.399290428612424	39.0	35.0	41.0	32.0	41.0
26	37.37041688561267	39.0	35.0	41.0	32.0	41.0
27	37.29683612155634	39.0	35.0	41.0	32.0	41.0
28	37.1934911235253	39.0	35.0	41.0	32.0	41.0
29	37.14299006326763	39.0	35.0	41.0	32.0	41.0
30	37.019180291008745	39.0	35.0	41.0	31.0	41.0
31	36.91505143522987	39.0	35.0	41.0	31.0	41.0
32	36.7894565542806	39.0	35.0	41.0	31.0	41.0
33	36.67884068557118	39.0	35.0	41.0	31.0	41.0
34	36.56008685789814	39.0	35.0	41.0	30.0	41.0
35	36.4639121085525	39.0	35.0	41.0	30.0	41.0
36	36.375380903933326	39.0	35.0	41.0	30.0	41.0
37	36.27451791506262	39.0	35.0	41.0	30.0	41.0
38	36.13841238371017	38.0	35.0	40.0	30.0	41.0
39	36.07698327811546	38.0	35.0	40.0	29.0	41.0
40	35.958782250280436	38.0	35.0	40.0	29.0	41.0
41	35.82679352491264	38.0	35.0	40.0	29.0	41.0
42	35.75081025549291	38.0	35.0	40.0	28.0	41.0
43	35.65394109017097	38.0	34.0	40.0	28.0	41.0
44	35.53059219148489	38.0	34.0	40.0	28.0	41.0
45	35.42975404755072	38.0	34.0	40.0	28.0	41.0
46	35.291433590105754	37.0	34.0	40.0	27.0	41.0
47	35.12387436909389	37.0	34.0	40.0	27.0	41.0
48	35.04503144747842	37.0	34.0	40.0	27.0	41.0
49	34.932695066913624	37.0	34.0	40.0	27.0	41.0
50	34.802659153557855	36.0	34.0	40.0	26.0	41.0
51	33.29911440223704	35.0	31.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	6.0
11	5.0
12	6.0
13	9.0
14	18.0
15	43.0
16	94.0
17	168.0
18	304.0
19	642.0
20	1117.0
21	1772.0
22	2472.0
23	3529.0
24	4554.0
25	5781.0
26	7235.0
27	8727.0
28	10818.0
29	12939.0
30	16140.0
31	20152.0
32	25805.0
33	35172.0
34	59339.0
35	71552.0
36	68812.0
37	97992.0
38	151862.0
39	197865.0
40	61.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.397014632425375	19.74066855239735	24.548287997535382	13.314028817641892
2	30.448836201060132	26.200103603385372	24.797482711029787	18.553577484524713
3	31.783009293248515	26.135382543699137	24.837855732906995	17.243752430145356
4	28.47503021765407	26.25575626123457	25.69823588528099	19.570977635830374
5	25.933268984947695	30.01790077677694	23.46107357455282	20.58775666372254
6	24.685680496600593	36.12913404215937	24.053128412296754	15.132057048943281
7	82.03661398297874	5.911604200284971	7.756092289001271	4.295689527735023
8	83.51824177353096	5.707006147879547	6.490988120393594	4.28376395819591
9	77.86129817277914	6.72999640990667	8.396967427045949	7.011737990268238
10	42.55515265350133	24.77586761624014	15.952561078170865	16.716418652087658
11	33.146250961188485	25.543327705955203	21.72801502621762	19.582406306638692
12	28.773666354862716	22.156093282798732	28.192294839830907	20.87794552250765
13	25.46220898815269	24.985186206588132	29.36460317046235	20.188001634796823
14	21.49621176830109	28.619751972998525	27.923969525200842	21.960066733499545
15	20.461917060148348	25.416494304919425	32.04611717120521	22.075471463727013
16	23.527036881066046	23.661696437111875	29.735041174271082	23.076225507550998
17	22.954361093823174	24.031761766872506	26.759487349579437	26.25438978972488
18	24.79288639776992	24.52071011797618	27.354896253756245	23.331507230497657
19	25.400345096168536	26.325694757594164	25.039223943562245	23.234736202675055
20	26.30867597606439	25.6691673095294	25.6557510437979	22.366405670608316
21	25.810534998440982	27.524959844371317	25.59301757903485	21.071487578152855
22	24.210024186545724	23.954618238916364	25.82705688123996	26.008300693297954
23	22.978212232901406	26.95427165205163	26.147183888555553	23.92033222649141
24	23.483930916169456	26.244824489157047	27.268560099280364	23.002684495393126
25	23.679833240785943	27.292908137089388	25.263201046468726	23.764057575655936
26	22.409014736774107	26.282713017380278	25.811156121854477	25.49711612399114
27	23.524552387412065	25.732521897705947	26.45240393394725	24.29052178093474
28	22.08702435921803	26.51662809490269	26.81427043464974	24.582077111229538
29	23.66927414275652	26.23898592907019	25.865690757559385	24.226049170613905
30	24.920837820949995	25.51550137703061	26.33737187776788	23.226288924251513
31	27.23377718812462	25.781093748641293	23.46852705551477	23.51660200771932
32	26.35289996310527	27.09638468905941	23.374985869442344	23.175729478392977
33	24.79238949903912	26.506317446238665	22.727775272580008	25.973517782142203
34	24.32803763510987	26.7089279037209	26.106189743264846	22.85684471790438
35	23.64840439606307	26.36109879216341	24.590772839018477	25.399723972755044
36	25.84556635896213	27.83688802262877	23.07833732715688	23.239208291252222
37	23.15523240574763	28.32347610476116	25.16456664840564	23.356724841085573
38	23.525918858921756	29.767463816455546	23.327407815968588	23.37920950865411
39	23.89126365073982	27.214398137623558	24.6009592629998	24.29337894863682
40	24.367292634842787	25.099100240623212	27.834900427705584	22.69870669682842
41	21.685530184734525	25.801715045969342	26.304700786218017	26.208053983078113
42	23.721821183538243	25.771279998708064	25.445065981940218	25.06183283581348
43	23.256599746830098	25.341214147203768	25.56320365518706	25.838982450779074
44	23.935114963732605	25.01052804185875	26.49873974059402	24.555617253814628
45	24.08132741526945	25.061584386448082	25.124069401845727	25.733018796436742
46	24.964440684577383	25.751900948207002	25.083447930603125	24.200210436612494
47	22.644047836440816	25.74308099573537	27.89962148739182	23.713249680432003
48	22.568891903407856	26.456503348476325	26.23202934683904	24.74257540127678
49	23.290016186476155	25.010031143127954	27.6934085141113	24.00654415628459
50	22.73982506680182	24.923694988652077	27.71912302343002	24.617356921116084
51	22.163919437808776	25.063447756688568	25.671776027866084	27.10085677763658
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	857.0
1	1028.0
2	1199.0
3	1546.0
4	1893.0
5	1460.5
6	1028.0
7	1015.5
8	1003.0
9	1078.0
10	1153.0
11	1214.5
12	1276.0
13	1329.5
14	1383.0
15	1445.5
16	1508.0
17	1622.0
18	1736.0
19	1625.0
20	1514.0
21	1716.0
22	1918.0
23	2276.5
24	2635.0
25	3419.5
26	5245.0
27	6286.0
28	7342.0
29	8398.0
30	9998.5
31	11599.0
32	13002.5
33	14406.0
34	16525.0
35	18644.0
36	20334.0
37	22024.0
38	23351.0
39	24678.0
40	27209.0
41	29740.0
42	34306.5
43	38873.0
44	43338.0
45	47803.0
46	57652.5
47	67502.0
48	68314.5
49	69127.0
50	67990.0
51	66853.0
52	61261.0
53	55669.0
54	51895.0
55	48121.0
56	46055.0
57	43989.0
58	42199.5
59	40410.0
60	38497.0
61	36584.0
62	34868.5
63	33153.0
64	30059.5
65	26966.0
66	23762.5
67	20559.0
68	17618.5
69	14678.0
70	13169.0
71	11660.0
72	10221.0
73	8782.0
74	7755.5
75	5033.5
76	3338.0
77	2785.0
78	2232.0
79	1932.0
80	1632.0
81	1130.5
82	629.0
83	434.0
84	239.0
85	222.5
86	206.0
87	164.0
88	122.0
89	79.0
90	36.0
91	22.5
92	9.0
93	9.0
94	9.0
95	4.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	804993.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.74150772481218
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.95074311120624	19.036036250527165
2	9.482906023781066	4.882085973296565
3	3.3919079795092926	2.6193847636917154
4	1.769810308096353	1.8223034286925799
5	1.1205776766614761	1.4422679460016736
6	0.8323826855406651	1.2856071197906964
7	0.6246190453124028	1.1255045185981816
8	0.5235691132531793	1.0781966698623824
9	0.4346632916836901	1.00699996325112
>10	6.6875141811648735	42.687029431994524
>50	1.105780425155578	17.784925702695183
>100	0.06777988595464962	3.071394493190152
>500	0.004841420425332116	0.8336197104868706
>1k	0.0029048522551992696	1.3246440279212062
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCG	2619	0.32534444398895396	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTTC	2308	0.2867105676695325	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGC	2200	0.2732943019380293	No Hit
GCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTTCTGC	1351	0.1678275463264898	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	1093	0.1357775781901209	No Hit
CCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTTCTGC	1058	0.13142971429565226	No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	914	0.11354135998698126	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	875	0.10869659736171618	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.050062547127739	0.0
2	0.0	0.0	0.0	0.47602898410296735	0.0
3	0.0	0.0	0.0	0.699633412961355	0.0
4	0.0	0.0	0.0	1.0493258947593334	0.0
5	0.0	0.0	0.0	2.206603038784188	0.0
6	1.242246826991042E-4	0.0	0.0	2.943006957824478	0.0
7	1.242246826991042E-4	0.0	0.0	3.586863488253935	0.0
8	1.242246826991042E-4	0.0	0.0	4.523144921757083	0.0
9	1.242246826991042E-4	0.0	0.0	4.897433890729484	0.0
10	1.242246826991042E-4	0.0	0.0	5.927877633718554	0.0
11	1.242246826991042E-4	0.0	0.0	6.885028813915152	0.0
12	1.242246826991042E-4	0.0	0.0	7.916963253096611	0.0
13	1.242246826991042E-4	0.0	0.0	8.24168657367207	0.0
14	2.484493653982084E-4	0.0	0.0	8.377961050592987	0.0
15	2.484493653982084E-4	0.0	0.0	8.634236571001239	0.0
16	2.484493653982084E-4	0.0	0.0	9.105048118430844	0.0
17	2.484493653982084E-4	0.0	0.0	9.662320045019024	0.0
18	2.484493653982084E-4	0.0	0.0	10.336114723978966	0.0
19	2.484493653982084E-4	0.0	0.0	10.744317031328222	0.0
20	2.484493653982084E-4	0.0	0.0	11.089661649231733	0.0
21	2.484493653982084E-4	0.0	0.0	11.472522121310371	0.0
22	2.484493653982084E-4	0.0	0.0	11.903209096228165	0.0
23	3.726740480973126E-4	0.0	0.0	12.30756043841375	0.0
24	3.726740480973126E-4	0.0	0.0	12.639861464633855	0.0
25	4.968987307964168E-4	0.0	0.0	12.94011252271759	0.0
26	4.968987307964168E-4	0.0	0.0	13.21713356513659	0.0
27	6.21123413495521E-4	0.0	0.0	13.531918911096122	0.0
28	6.21123413495521E-4	0.0	0.0	13.810306425024814	0.0
29	6.21123413495521E-4	0.0	0.0	14.13391172345598	0.0
30	6.21123413495521E-4	0.0	0.0	14.493293730504488	0.0
31	6.21123413495521E-4	0.0	0.0	14.806215706223533	0.0
32	6.21123413495521E-4	0.0	0.0	15.106218314941868	0.0
33	6.21123413495521E-4	0.0	0.0	15.411686809698967	0.0
34	6.21123413495521E-4	0.0	0.0	15.727590177802789	0.0
35	6.21123413495521E-4	0.0	0.0	16.077034210235368	0.0
36	6.21123413495521E-4	0.0	0.0	16.37082558481875	0.0
37	7.453480961946252E-4	0.0	0.0	16.692443288326732	0.0
38	7.453480961946252E-4	0.0	0.0	17.01977532723887	0.0
39	7.453480961946252E-4	0.0	0.0	17.420399928943482	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATACG	20	7.0320244E-4	45.000004	1
CAATCGA	20	7.0320244E-4	45.000004	28
CGTCACA	20	7.0320244E-4	45.000004	23
GCGAATG	35	1.2113196E-7	45.0	1
ACCGAAC	25	3.889761E-5	45.0	10
TAGTTCG	25	3.889761E-5	45.0	40
TCGACAC	25	3.889761E-5	45.0	34
AGATCGG	50	1.0804797E-9	40.5	2
GCGTACG	50	1.0804797E-9	40.5	1
TTTAACG	40	3.4564982E-7	39.375004	39
TTACGGG	160	0.0	39.375004	3
AATAGCG	35	6.2459585E-6	38.571426	1
AAGGGAT	555	0.0	38.51351	5
GCTACGA	135	0.0	38.333336	10
TTGAGCG	65	9.094947E-12	38.076927	1
TATGCGG	90	0.0	37.5	2
GCATCCG	30	1.139618E-4	37.499996	28
ACGGGCT	250	0.0	36.9	5
GCGATAT	55	2.746674E-9	36.81818	9
GTACGAG	55	2.746674E-9	36.81818	1
>>END_MODULE