FastQCFastQC Report
Sat 18 Jun 2016
SRR3554047_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554047_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences762980
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCTTCTGC19120.25059634590683894No Hit
CTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCTTCTGCT10010.13119609950457417No Hit
CCTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCTTCTGC9170.12018663660908542No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC9030.11835172612650398No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA7990.10472096254161316No Hit
TCTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCTTCTGC7740.10144433667986054No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA7640.10013368633515951No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTAA302.1645374E-645.00000427
TGCGTAG600.045.0000041
TCGTTGC406.8102963E-945.016
GAATGCG351.2112105E-745.01
TAGCGTG207.031794E-445.02
CACGCGT207.031794E-445.039
TTATCGG207.031794E-445.02
TACGACA207.031794E-445.01
CGAATAT750.045.014
ACGTAAG207.031794E-445.01
TGTACGC207.031794E-445.036
ATACCGT207.031794E-445.041
GACTATC207.031794E-445.039
CGTCAGG1000.042.752
CTACGAA850.042.3529411
TTGAGCG750.042.01
TTAGTCA1200.041.25000432
GTTAGCG556.002665E-1140.909091
GACACGA3800.040.85526725
ATTGGGC1050.040.7142874