##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554047_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	762980
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.08746231880259	33.0	31.0	34.0	30.0	34.0
2	32.18501926656007	34.0	31.0	34.0	30.0	34.0
3	32.21217332040158	34.0	31.0	34.0	30.0	34.0
4	35.78454612178563	37.0	35.0	37.0	35.0	37.0
5	35.65706833730897	37.0	35.0	37.0	33.0	37.0
6	35.648102178300874	37.0	35.0	37.0	33.0	37.0
7	35.86521665050198	37.0	35.0	37.0	35.0	37.0
8	35.70450339458439	37.0	35.0	37.0	35.0	37.0
9	37.39608246611969	39.0	37.0	39.0	35.0	39.0
10	37.002918818317646	39.0	37.0	39.0	33.0	39.0
11	36.966345120448764	39.0	37.0	39.0	33.0	39.0
12	36.83036383653569	39.0	35.0	39.0	33.0	39.0
13	36.765818239010194	39.0	35.0	39.0	33.0	39.0
14	37.88680175102886	40.0	37.0	41.0	33.0	41.0
15	38.02442921177488	40.0	37.0	41.0	33.0	41.0
16	38.07943065349026	40.0	37.0	41.0	33.0	41.0
17	38.029526330965425	40.0	37.0	41.0	33.0	41.0
18	38.00276154027628	40.0	36.0	41.0	33.0	41.0
19	37.94566305800939	40.0	36.0	41.0	33.0	41.0
20	37.89426066214055	40.0	36.0	41.0	33.0	41.0
21	37.7683019214134	40.0	36.0	41.0	33.0	41.0
22	37.74671419958583	40.0	36.0	41.0	33.0	41.0
23	37.68601273952135	40.0	36.0	41.0	33.0	41.0
24	37.67143961833862	40.0	36.0	41.0	33.0	41.0
25	37.57025479042701	39.0	35.0	41.0	33.0	41.0
26	37.53779260268946	39.0	35.0	41.0	33.0	41.0
27	37.48212797189966	39.0	35.0	41.0	33.0	41.0
28	37.37216702927993	39.0	35.0	41.0	32.0	41.0
29	37.34176518388424	39.0	35.0	41.0	32.0	41.0
30	37.212635979973264	39.0	35.0	41.0	32.0	41.0
31	37.12764816902147	39.0	35.0	41.0	32.0	41.0
32	37.014195653883455	39.0	35.0	41.0	31.0	41.0
33	36.94635508139139	39.0	35.0	41.0	31.0	41.0
34	36.84622401635691	39.0	35.0	41.0	31.0	41.0
35	36.77227057065716	39.0	35.0	41.0	31.0	41.0
36	36.704018453956856	39.0	35.0	41.0	31.0	41.0
37	36.61947102152088	39.0	35.0	41.0	31.0	41.0
38	36.49506933340324	39.0	35.0	41.0	30.0	41.0
39	36.47110278120003	39.0	35.0	41.0	30.0	41.0
40	36.29336548795512	38.0	35.0	40.0	30.0	41.0
41	36.20297648693282	38.0	35.0	40.0	30.0	41.0
42	36.1177829038769	38.0	35.0	40.0	30.0	41.0
43	36.032227581325856	38.0	35.0	40.0	30.0	41.0
44	35.89922802694697	38.0	35.0	40.0	29.0	41.0
45	35.81368712154971	38.0	35.0	40.0	29.0	41.0
46	35.66698602846733	38.0	34.0	40.0	29.0	41.0
47	35.57392461139217	38.0	34.0	40.0	29.0	41.0
48	35.47789588193662	37.0	34.0	40.0	28.0	41.0
49	35.413380429369056	37.0	34.0	40.0	28.0	41.0
50	35.32671498597604	37.0	34.0	40.0	28.0	41.0
51	33.88056174473774	36.0	32.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	2.0
10	7.0
11	4.0
12	8.0
13	9.0
14	18.0
15	41.0
16	96.0
17	164.0
18	261.0
19	471.0
20	840.0
21	1212.0
22	1820.0
23	2569.0
24	3536.0
25	4523.0
26	5806.0
27	7172.0
28	8918.0
29	10966.0
30	13831.0
31	17624.0
32	22914.0
33	31623.0
34	53639.0
35	68652.0
36	65421.0
37	92661.0
38	148364.0
39	199740.0
40	66.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.16183910456368	18.857899289627515	24.25673018952004	12.723531416288763
2	29.990432252483686	24.615717318933655	26.424283729586623	18.969566698996044
3	33.137434795145346	24.739966971611313	24.890298566148523	17.232299667094814
4	29.753204540092792	25.99688065217961	24.10469474953472	20.145220058192876
5	25.0280479173766	31.509869197095597	23.13769692521429	20.324385960313506
6	25.1003958164041	35.98940994521482	23.301266088232982	15.608928150148104
7	82.84660148365619	6.128076751684185	6.479068913995125	4.5462528506645
8	84.36092689192377	5.148889879158038	5.6440535793860915	4.846129649532098
9	78.48239796587066	7.357335709979291	8.402710424912842	5.757555899237202
10	40.603554483734825	27.18826181551286	15.412592728511887	16.795590972240426
11	32.72182757084065	27.063881097800728	20.461873181472647	19.752418149885973
12	28.055257018532597	24.31924821096228	27.437154315971586	20.18834045453354
13	24.57154840231723	26.934913103882145	28.156177095074575	20.33736139872605
14	21.00736585493722	29.842459828566938	27.344884531704633	21.805289784791213
15	20.889669453983068	26.67632179087263	31.79559097224043	20.638417782903876
16	22.90715352958138	24.84848882015256	30.269469710870535	21.97488793939553
17	22.902697318409395	24.35699494088967	27.17790767778972	25.562400062911216
18	25.18663660908543	24.996985504207185	26.769771160449817	23.04660672625757
19	25.851398463917796	27.333875068809142	24.493957901910928	22.320768565362133
20	27.70033290518755	25.722430469999214	24.160135259115574	22.417101365697658
21	25.516265170777743	27.364937481978558	24.907599150698577	22.211198196545126
22	23.895252824451493	24.9912186426905	24.629741277621957	26.483787255236045
23	23.792891032530342	27.986447875435793	24.30535531730845	23.915305774725418
24	23.1100422029411	25.993472961283388	27.62025216912632	23.27623266664919
25	23.125901072111983	26.6457836378411	25.83186977378175	24.39644551626517
26	22.866392303861176	28.04673779129204	24.853862486565834	24.233007418280952
27	23.833390128181602	27.23098901675011	25.23788303756324	23.697737817505047
28	21.241448006500825	28.094183333770218	27.28682272143438	23.37754593829458
29	23.010957036881702	27.019318986080894	25.397520249547828	24.57220372748958
30	24.476788382395345	26.695850481008677	25.18834045453354	23.63902068206244
31	26.14970248237175	27.661013394846524	22.07436630055834	24.114917822223386
32	24.517942803218958	28.81753125901072	22.93703635744056	23.72748958032976
33	24.6085087420378	27.86914466958505	22.39364072452751	25.128705863849643
34	22.921046423235207	27.487876484311514	24.53209782694173	25.05897926551155
35	22.73454087918425	27.708327872290226	24.51650108783979	25.040630160685733
36	25.317832708590004	28.434559228289075	23.301003958164042	22.94660410495688
37	23.481087315525965	29.085821384571027	24.5322288919762	22.900862407926816
38	23.11331882880285	30.013761828619362	24.05528323153949	22.817636111038297
39	24.908254475870926	25.802904401163858	24.618207554588587	24.67063356837663
40	24.30850087813573	25.296206977902436	27.560748643476895	22.83454350048494
41	21.55928071509083	26.249705103672444	25.93659073632336	26.254423444913368
42	22.85787307662062	26.673831555217696	25.633044116490606	24.835251251671078
43	23.874282418936275	25.398699834858057	25.895174185430808	24.831843560774857
44	24.411386930194762	25.501323756848148	25.672494691866106	24.414794621090984
45	24.21610002883431	25.73134289234318	24.803402448294847	25.24915463052767
46	24.629217017484077	26.08469422527458	24.966054156072243	24.320034601169098
47	22.729036147736505	26.755485071692576	26.40082308841647	24.114655692154447
48	22.84987810951794	26.455346142756035	26.02427324438386	24.67050250334216
49	23.734173897087736	25.58179768801279	27.123122493381214	23.56090592151826
50	21.573566803848067	25.574064850979056	27.075676950903038	25.776691394269836
51	22.29337597315788	25.811554693438882	25.29397887231644	26.60109046108679
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	874.0
1	1067.5
2	1261.0
3	1424.0
4	1587.0
5	1227.5
6	868.0
7	958.5
8	1049.0
9	1095.5
10	1142.0
11	1119.5
12	1097.0
13	1152.0
14	1207.0
15	1355.0
16	1503.0
17	1368.5
18	1234.0
19	1316.0
20	1398.0
21	1912.5
22	2427.0
23	2462.0
24	2497.0
25	3220.0
26	4881.5
27	5820.0
28	6905.5
29	7991.0
30	10251.5
31	12512.0
32	13399.5
33	14287.0
34	15116.0
35	15945.0
36	16154.5
37	16364.0
38	19587.5
39	22811.0
40	26129.5
41	29448.0
42	33641.5
43	37835.0
44	42447.5
45	47060.0
46	52712.0
47	58364.0
48	64160.5
49	69957.0
50	69962.5
51	69968.0
52	63764.0
53	57560.0
54	52329.0
55	47098.0
56	44406.0
57	41714.0
58	40370.5
59	39027.0
60	37071.5
61	35116.0
62	31878.5
63	28641.0
64	25473.0
65	22305.0
66	19710.5
67	17116.0
68	14825.5
69	12535.0
70	11371.0
71	10207.0
72	8897.5
73	7588.0
74	6801.0
75	4673.0
76	3332.0
77	2675.5
78	2019.0
79	1576.5
80	1134.0
81	876.0
82	618.0
83	471.0
84	324.0
85	213.5
86	103.0
87	61.5
88	20.0
89	16.5
90	13.0
91	16.0
92	19.0
93	19.0
94	19.0
95	13.5
96	8.0
97	4.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	762980.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.298742223883075
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.83115758600118	17.94004266273132
2	9.970760657370674	4.845538859789699
3	3.6550220578088948	2.6643731641591497
4	1.8017562466331078	1.7512164234883592
5	1.1107032315906047	1.3494345755827002
6	0.7712080579704987	1.1243631480963974
7	0.5879043692519393	0.9999735704523209
8	0.455042842548527	0.8845574985527735
9	0.3853931460883633	0.8428111840487206
>10	5.648081240422108	34.84742233330348
>50	1.6637445691319679	26.61894113982705
>100	0.11055501371430425	4.504241461182275
>500	0.007587108784314997	1.2434893178758726
>1k	0.001083872683473571	0.38359466090992445
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCTTCTGC	1912	0.25059634590683894	No Hit
CTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCTTCTGCT	1001	0.13119609950457417	No Hit
CCTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCTTCTGC	917	0.12018663660908542	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	903	0.11835172612650398	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	799	0.10472096254161316	No Hit
TCTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCTTCTGC	774	0.10144433667986054	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	764	0.10013368633515951	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.3106503447010408E-4	0.0	0.0	0.13958426171066082	0.0
2	1.3106503447010408E-4	0.0	0.0	0.7114210071037249	0.0
3	1.3106503447010408E-4	0.0	0.0	0.9592649872866916	0.0
4	1.3106503447010408E-4	0.0	0.0	1.2955778657369788	0.0
5	1.3106503447010408E-4	0.0	0.0	2.2568088285407217	0.0
6	1.3106503447010408E-4	0.0	0.0	2.9897245012975437	0.0
7	1.3106503447010408E-4	0.0	0.0	3.6276180240635405	0.0
8	1.3106503447010408E-4	0.0	0.0	4.686230307478571	0.0
9	1.3106503447010408E-4	0.0	0.0	5.133031009987156	0.0
10	1.3106503447010408E-4	0.0	0.0	6.005137749351228	0.0
11	1.3106503447010408E-4	0.0	0.0	7.026789693045689	0.0
12	1.3106503447010408E-4	0.0	0.0	7.80819942855645	0.0
13	1.3106503447010408E-4	0.0	0.0	8.141104616110514	0.0
14	1.3106503447010408E-4	0.0	0.0	8.264436813546881	0.0
15	1.3106503447010408E-4	0.0	0.0	8.503892631523762	0.0
16	1.3106503447010408E-4	0.0	0.0	9.046501874229993	0.0
17	1.3106503447010408E-4	0.0	0.0	9.675351909617552	0.0
18	1.3106503447010408E-4	0.0	0.0	10.419670240373273	0.0
19	1.3106503447010408E-4	0.0	0.0	10.778919499855828	0.0
20	1.3106503447010408E-4	0.0	0.0	11.15835277464678	0.0
21	1.3106503447010408E-4	0.0	0.0	11.616949330257674	0.0
22	1.3106503447010408E-4	0.0	0.0	12.072924585179166	0.0
23	1.3106503447010408E-4	0.0	0.0	12.550918765891636	0.0
24	1.3106503447010408E-4	0.0	0.0	12.929565650475766	0.0
25	1.3106503447010408E-4	0.0	0.0	13.239010196859681	0.0
26	1.3106503447010408E-4	0.0	0.0	13.535872499934467	0.0
27	1.3106503447010408E-4	0.0	0.0	13.835880363836536	0.0
28	1.3106503447010408E-4	0.0	0.0	14.1573828933917	0.0
29	1.3106503447010408E-4	0.0	0.0	14.498545178117382	0.0
30	1.3106503447010408E-4	0.0	0.0	14.897113947940968	0.0
31	1.3106503447010408E-4	0.0	0.0	15.2503342158379	0.0
32	1.3106503447010408E-4	0.0	0.0	15.608141759941283	0.0
33	1.3106503447010408E-4	0.0	0.0	15.942357597840049	0.0
34	1.3106503447010408E-4	0.0	0.0	16.274869590290702	0.0
35	2.6213006894020816E-4	0.0	0.0	16.648142808461557	0.0
36	2.6213006894020816E-4	0.0	0.0	17.000576686151668	0.0
37	2.6213006894020816E-4	0.0	0.0	17.326404361844347	0.0
38	2.6213006894020816E-4	0.0	0.0	17.653935882985138	0.0
39	2.6213006894020816E-4	0.0	0.0	17.992870062124826	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGTAA	30	2.1645374E-6	45.000004	27
TGCGTAG	60	0.0	45.000004	1
TCGTTGC	40	6.8102963E-9	45.0	16
GAATGCG	35	1.2112105E-7	45.0	1
TAGCGTG	20	7.031794E-4	45.0	2
CACGCGT	20	7.031794E-4	45.0	39
TTATCGG	20	7.031794E-4	45.0	2
TACGACA	20	7.031794E-4	45.0	1
CGAATAT	75	0.0	45.0	14
ACGTAAG	20	7.031794E-4	45.0	1
TGTACGC	20	7.031794E-4	45.0	36
ATACCGT	20	7.031794E-4	45.0	41
GACTATC	20	7.031794E-4	45.0	39
CGTCAGG	100	0.0	42.75	2
CTACGAA	85	0.0	42.35294	11
TTGAGCG	75	0.0	42.0	1
TTAGTCA	120	0.0	41.250004	32
GTTAGCG	55	6.002665E-11	40.90909	1
GACACGA	380	0.0	40.855267	25
ATTGGGC	105	0.0	40.714287	4
>>END_MODULE