SRR3554046_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3554046_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1159559
Sequences flagged as poor quality	0
Sequence length	51
%GC	50

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTTCTGC	2886	0.24888772369495646	TruSeq Adapter, Index 22 (95% over 23bp)
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	2052	0.17696382848996903	No Hit
CCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTTCTGC	1840	0.15868101579997224	TruSeq Adapter, Index 22 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTTCTGCT	1634	0.14091564120497535	TruSeq Adapter, Index 20 (95% over 22bp)
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	1327	0.11444005867747999	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1269	0.10943815709248085	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	1179	0.1016765856674822	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATGCGT	20	7.033312E-4	45.000004	20
AGATTCG	20	7.033312E-4	45.000004	6
TTACGCC	20	7.033312E-4	45.000004	19
ATTGCGA	20	7.033312E-4	45.000004	30
TGTAACG	30	2.1655178E-6	45.000004	1
CGATATC	20	7.033312E-4	45.000004	10
CCGAGTA	25	3.8908278E-5	45.0	23
TTACGAG	25	3.8908278E-5	45.0	1
ATTACGG	65	0.0	44.999996	2
TATAGCG	70	0.0	41.785713	1
CGTGTAC	330	0.0	39.545456	18
ATAGCGG	160	0.0	39.375004	2
TATGCCC	335	0.0	38.955223	10
CGAAAGG	70	0.0	38.571426	2
TACCGGC	35	6.2483377E-6	38.571426	31
TTACGGG	275	0.0	38.454544	3
CCCTCGT	340	0.0	38.38235	14
GTGCTAG	100	0.0	38.25	1
ACTATGC	335	0.0	37.611942	8
CGTTAGA	30	1.139928E-4	37.500004	33