FastQCFastQC Report
Sat 18 Jun 2016
SRR3554043_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554043_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences948948
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTCTTCTGC21290.2243537053663636No Hit
CCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTCTTCTGC15830.16681630605681239No Hit
CTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTCTTCTGCT15050.15859667758401935No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT12320.12982797792924375No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC10740.1131779612792271No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA10450.11012194556498354No Hit
TCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTCTTCTGC9740.10263997605769759No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATCG302.1650994E-645.0000042
TGTAACG302.1650994E-645.0000041
TGTATAC253.89029E-545.044
CGCCTAT253.89029E-545.027
CTAACGG207.0326636E-445.02
TACGAAC207.0326636E-445.039
GTATACG207.0326636E-445.01
TATAGCG406.8121153E-945.01
TGTCGAT207.0326636E-445.036
TACGAAT1750.043.71428712
GCTACGA1750.043.71428710
CGTATGG603.6379788E-1241.2500042
ACCGCGT501.0804797E-940.525
ACCACGC501.0804797E-940.517
CGTAAGG1700.039.7058832
CGTTTGG1200.039.3750042
TCGTTTA403.4573713E-739.37538
CGAATAT1950.039.2307714
ACGCATT1150.039.13043217
AATCTCG356.247137E-638.57142618