##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554042_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	589395
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.122652889827705	33.0	31.0	34.0	30.0	34.0
2	32.23731453439544	34.0	31.0	34.0	30.0	34.0
3	32.27125102859712	34.0	31.0	34.0	30.0	34.0
4	35.817489120199525	37.0	35.0	37.0	35.0	37.0
5	35.686802568735736	37.0	35.0	37.0	33.0	37.0
6	35.69161258578712	37.0	35.0	37.0	33.0	37.0
7	35.891147702304906	37.0	35.0	37.0	35.0	37.0
8	35.77292138548851	37.0	35.0	37.0	35.0	37.0
9	37.429587967322426	39.0	37.0	39.0	35.0	39.0
10	37.051086283392294	39.0	37.0	39.0	33.0	39.0
11	37.01513755630774	39.0	37.0	39.0	33.0	39.0
12	36.81349010425945	39.0	35.0	39.0	33.0	39.0
13	36.693921733302794	39.0	35.0	39.0	33.0	39.0
14	37.745489866727745	40.0	36.0	41.0	33.0	41.0
15	37.899759923311194	40.0	36.0	41.0	33.0	41.0
16	37.981767744890945	40.0	36.0	41.0	33.0	41.0
17	37.96223585201775	40.0	36.0	41.0	33.0	41.0
18	37.95598028486838	40.0	36.0	41.0	33.0	41.0
19	37.9265042967789	40.0	36.0	41.0	33.0	41.0
20	37.84302717193054	40.0	36.0	41.0	33.0	41.0
21	37.724721112327046	40.0	36.0	41.0	33.0	41.0
22	37.72629221489833	40.0	36.0	41.0	33.0	41.0
23	37.68869603576549	40.0	35.0	41.0	33.0	41.0
24	37.67125442190721	40.0	35.0	41.0	33.0	41.0
25	37.55915642311184	40.0	35.0	41.0	33.0	41.0
26	37.46742846478168	39.0	35.0	41.0	33.0	41.0
27	37.41059221744331	39.0	35.0	41.0	33.0	41.0
28	37.322993917491665	39.0	35.0	41.0	32.0	41.0
29	37.26576743949304	39.0	35.0	41.0	32.0	41.0
30	37.17825227563858	39.0	35.0	41.0	32.0	41.0
31	37.08192298882753	39.0	35.0	41.0	32.0	41.0
32	36.97480806589808	39.0	35.0	41.0	31.0	41.0
33	36.878002019019505	39.0	35.0	41.0	31.0	41.0
34	36.74737824379236	39.0	35.0	41.0	31.0	41.0
35	36.685898251596974	39.0	35.0	41.0	31.0	41.0
36	36.562800838147595	39.0	35.0	41.0	31.0	41.0
37	36.43866846512101	39.0	35.0	41.0	30.0	41.0
38	36.3020894306874	38.0	35.0	41.0	30.0	41.0
39	36.229725396381035	38.0	35.0	40.0	30.0	41.0
40	36.04698037818441	38.0	35.0	40.0	30.0	41.0
41	35.96277708497697	38.0	35.0	40.0	30.0	41.0
42	35.84433189965982	38.0	35.0	40.0	29.0	41.0
43	35.74287192799396	38.0	35.0	40.0	29.0	41.0
44	35.61433503847165	38.0	34.0	40.0	29.0	41.0
45	35.5572442928766	38.0	34.0	40.0	28.0	41.0
46	35.452435124152736	37.0	34.0	40.0	28.0	41.0
47	35.33913250027571	37.0	34.0	40.0	28.0	41.0
48	35.20690538603144	37.0	34.0	40.0	27.0	41.0
49	35.118355262599785	37.0	34.0	40.0	27.0	41.0
50	34.98288414391028	37.0	34.0	40.0	27.0	41.0
51	33.49555052214559	35.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	4.0
11	4.0
12	6.0
13	13.0
14	12.0
15	35.0
16	83.0
17	105.0
18	220.0
19	396.0
20	602.0
21	1030.0
22	1469.0
23	2216.0
24	3012.0
25	3868.0
26	4849.0
27	6019.0
28	7437.0
29	9095.0
30	11132.0
31	13868.0
32	17879.0
33	24599.0
34	43258.0
35	55543.0
36	48862.0
37	70725.0
38	111542.0
39	151459.0
40	51.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.75436676592099	19.31336370345863	23.37006591504848	12.562203615571901
2	29.465808159214106	25.290679425512604	26.405212124296952	18.83830029097634
3	33.373374392385415	24.317647757446196	25.24503940481341	17.06393844535498
4	28.58185088098813	26.685669203165958	25.27439153708464	19.458088378761275
5	24.60913309410497	30.870638536126027	24.188362643049228	20.331865726719773
6	24.62406365849727	36.83472034883228	23.2404414696426	15.300774523027853
7	83.85853290238295	5.897912265967645	6.090143282518515	4.1534115491308885
8	85.98834397984373	4.365832760712256	5.325121522917568	4.320701736526438
9	80.17509480060062	7.257611618693745	7.507019910246948	5.060273670458691
10	42.807285436761426	26.24861086368225	14.478914819433486	16.465188880122838
11	34.81756716633158	26.17141305915388	19.669830928324807	19.341188846189734
12	30.496186767787307	22.589265263532944	25.902832565596928	21.011715403082825
13	25.92404075365417	26.685669203165958	27.007354999618254	20.382935043561616
14	22.028181440290467	28.598817431433925	27.695348620195286	21.67765250808032
15	21.238388517038658	25.081142527507023	32.1242969485659	21.556172006888417
16	23.74960764852094	23.080955895452114	30.762561609786303	22.406874846240637
17	23.256899023575023	23.12964989523155	26.73724751652118	26.876203564672245
18	25.54874065779316	24.197354914785503	27.135452455484018	23.118451971937326
19	26.7537050704536	26.789334826389773	23.863962198525606	22.59299790463102
20	28.142417224442013	25.057050025873988	24.163930810407283	22.636601939276716
21	26.38417360174416	26.1463025644941	24.61541071776992	22.854113115991822
22	24.752839776380863	24.30644983415197	24.322398391571017	26.61831199789615
23	23.457443649844333	27.585405373306525	24.127792057957738	24.829358918891405
24	22.839691548112896	25.745382977459936	27.99277224951009	23.422153224917075
25	24.448799192392197	26.295947539426024	25.360411947844824	23.89484132033696
26	23.41350028418972	26.870434937520677	25.0243045835136	24.691760194776
27	23.317469608666514	26.735211530467684	25.873819764334616	24.07349909653119
28	22.13032007397416	27.56589384029386	27.114753263940145	23.189032821791837
29	23.07722325435404	26.475623308647002	26.23826126791031	24.208892169088642
30	24.662747393513687	25.06366698054785	26.467988360946393	23.805597264992066
31	26.14850821605205	26.77627058254651	23.512924269802085	23.562296931599352
32	26.814954317562922	26.44745883490698	24.282357332518938	22.455229515011155
33	26.42828663290323	26.29187556731903	23.197516097014738	24.082321702763
34	22.92672995189983	27.249298008975302	25.678704434207962	24.145267604916906
35	24.677338626897072	25.519388525521936	26.003444209740493	23.7998286378405
36	25.94338262116238	28.436277878163203	24.052799904987317	21.5675395956871
37	24.99138947564876	28.08761526650209	24.123041423832912	22.797953834016237
38	26.06910475996573	28.890642099101623	23.079768236920913	21.96048490401174
39	26.26082678000322	25.847351945639176	23.26131032669093	24.630510947666675
40	26.058415833184878	24.40536482325096	26.72740691726262	22.808812426301547
41	22.858524419107727	24.193791939191883	26.09065227903189	26.857031362668497
42	24.631868271702338	25.56791285979691	25.224679544278455	24.575539324222294
43	24.172244420125722	24.150357570050645	26.296456535939395	25.38094147388424
44	24.628644627117637	25.098618074466188	25.96900211233553	24.30373518608064
45	24.376352021988648	25.948981582809495	24.528711644991898	25.14595475020996
46	24.206856183035146	26.08895562398731	24.98952315509972	24.714665037877822
47	23.96830648376725	25.642226350749496	26.234019630298867	24.155447535184386
48	24.032949040965736	26.417937037131296	25.86313083755376	23.685983084349203
49	24.88229455628229	25.111512652804997	26.548240144555006	23.457952646357704
50	22.428422365306798	25.33733743923854	27.097956378998806	25.136283816455858
51	22.720077367470033	26.61033771918662	24.553991805156134	26.115593108187205
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	478.0
1	647.0
2	816.0
3	998.5
4	1181.0
5	918.0
6	655.0
7	702.5
8	750.0
9	755.5
10	761.0
11	793.5
12	826.0
13	822.5
14	819.0
15	898.0
16	977.0
17	952.5
18	928.0
19	1000.5
20	1073.0
21	1175.0
22	1277.0
23	1422.5
24	1568.0
25	2090.5
26	2987.0
27	3361.0
28	4601.5
29	5842.0
30	7240.0
31	8638.0
32	8848.5
33	9059.0
34	10705.0
35	12351.0
36	13546.5
37	14742.0
38	15197.5
39	15653.0
40	19458.0
41	23263.0
42	27093.5
43	30924.0
44	34585.5
45	38247.0
46	41542.0
47	44837.0
48	47785.0
49	50733.0
50	49003.0
51	47273.0
52	43809.0
53	40345.0
54	38347.0
55	36349.0
56	33942.5
57	31536.0
58	31634.0
59	31732.0
60	30779.0
61	29826.0
62	27760.5
63	25695.0
64	22491.5
65	19288.0
66	17049.5
67	14811.0
68	13137.5
69	11464.0
70	10146.5
71	8829.0
72	7882.5
73	6936.0
74	6269.0
75	4390.0
76	3178.0
77	2625.0
78	2072.0
79	1555.5
80	1039.0
81	777.0
82	515.0
83	409.5
84	304.0
85	235.5
86	167.0
87	102.0
88	37.0
89	27.5
90	18.0
91	11.0
92	4.0
93	2.5
94	1.0
95	1.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	589395.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.11882300766393
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.84445692977971	18.800046667238625
2	9.07164126389467	4.557379025935818
3	3.303372343859524	2.4893047570145796
4	1.7365514269889564	1.744805117529673
5	1.066512165614644	1.33947651617973
6	0.7448799094172414	1.1226303963969857
7	0.575153526256053	1.011302573978171
8	0.5168697130332932	1.0386527071764269
9	0.3905313477729767	0.8828719023288467
>10	6.145679306140298	38.70824190978352
>50	1.5345386904255487	24.639691261764714
>100	0.06506877839260278	2.867269941953397
>500	0.004744598424460619	0.7983272227195226
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGCCGTCTTCTGC	919	0.15592259859686627	No Hit
GGGTCAGGGAGGGTCATTTCCACATTTCATTTACACACAGAACTAAACAGA	904	0.15337761602999686	No Hit
CTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGCCGTCTTCTGCT	614	0.10417461973718813	Illumina Single End Adapter 1 (95% over 21bp)
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	590	0.10010264763019706	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.6966550445796113E-4	0.0	0.0	0.1213108356874422	0.0
2	1.6966550445796113E-4	0.0	0.0	0.5447959348145132	0.0
3	1.6966550445796113E-4	0.0	0.0	0.728543676142485	0.0
4	1.6966550445796113E-4	0.0	0.0	0.9608157517454339	0.0
5	1.6966550445796113E-4	0.0	0.0	1.6082593167570136	0.0
6	1.6966550445796113E-4	0.0	0.0	2.22685974601074	0.0
7	1.6966550445796113E-4	0.0	0.0	2.7557071234062045	0.0
8	1.6966550445796113E-4	0.0	0.0	3.7290781224815275	0.0
9	3.3933100891592226E-4	0.0	0.0	4.149339577023897	0.0
10	3.3933100891592226E-4	0.0	0.0	4.940829155320286	0.0
11	3.3933100891592226E-4	0.0	0.0	5.806123228055887	0.0
12	3.3933100891592226E-4	0.0	0.0	6.543319844925729	0.0
13	3.3933100891592226E-4	0.0	0.0	6.830224212964141	0.0
14	3.3933100891592226E-4	0.0	0.0	6.9457664215000126	0.0
15	3.3933100891592226E-4	0.0	0.0	7.1427480721757055	0.0
16	3.3933100891592226E-4	0.0	0.0	7.562330864700243	0.0
17	3.3933100891592226E-4	0.0	0.0	8.09270523163583	0.0
18	3.3933100891592226E-4	0.0	0.0	8.770349256440927	0.0
19	3.3933100891592226E-4	0.0	0.0	9.073371847402845	0.0
20	3.3933100891592226E-4	0.0	0.0	9.387762027163447	0.0
21	3.3933100891592226E-4	0.0	0.0	9.740157279922633	0.0
22	3.3933100891592226E-4	0.0	0.0	10.11732369633268	0.0
23	3.3933100891592226E-4	0.0	0.0	10.502803722461168	0.0
24	3.3933100891592226E-4	0.0	0.0	10.808371295989955	0.0
25	3.3933100891592226E-4	0.0	0.0	11.081193427158357	0.0
26	3.3933100891592226E-4	0.0	0.0	11.315501488814801	0.0
27	3.3933100891592226E-4	0.0	0.0	11.599182212268513	0.0
28	3.3933100891592226E-4	0.0	0.0	11.855377124000034	0.0
29	3.3933100891592226E-4	0.0	0.0	12.141263499011698	0.0
30	3.3933100891592226E-4	0.0	0.0	12.506722995614147	0.0
31	3.3933100891592226E-4	0.0	0.0	12.782599105862792	0.0
32	3.3933100891592226E-4	0.0	0.0	13.080871062699886	0.0
33	3.3933100891592226E-4	0.0	0.0	13.358104496984195	0.0
34	3.3933100891592226E-4	0.0	0.0	13.631605290170429	0.0
35	3.3933100891592226E-4	0.0	0.0	13.952951755613807	0.0
36	3.3933100891592226E-4	0.0	0.0	14.23476615851848	0.0
37	5.089965133738834E-4	0.0	0.0	14.54270904910968	0.0
38	5.089965133738834E-4	0.0	0.0	14.84810695713401	0.0
39	5.089965133738834E-4	0.0	0.0	15.249535540681546	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGAAAT	20	7.0304866E-4	45.0	33
AAACGCG	20	7.0304866E-4	45.0	1
CCCCGTG	20	7.0304866E-4	45.0	12
CTAAACG	20	7.0304866E-4	45.0	19
CTACGAC	20	7.0304866E-4	45.0	34
AACGCTA	20	7.0304866E-4	45.0	36
CCATAGT	20	7.0304866E-4	45.0	37
GATATCG	20	7.0304866E-4	45.0	2
TATTAGT	55	1.8189894E-12	45.0	30
ACGCTAC	20	7.0304866E-4	45.0	37
CATGTCG	20	7.0304866E-4	45.0	31
CATCGTT	40	6.8048394E-9	45.0	36
CTAGGCG	20	7.0304866E-4	45.0	1
ATCGACT	20	7.0304866E-4	45.0	28
TACGTCG	20	7.0304866E-4	45.0	10
ATCGACG	35	1.2105738E-7	45.0	1
TAATCGA	20	7.0304866E-4	45.0	33
ACATCGG	30	2.1636952E-6	44.999996	2
ATACGAG	30	2.1636952E-6	44.999996	1
TAATGCG	25	3.8884857E-5	44.999996	1
>>END_MODULE