##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554040_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	599095
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.030367470935325	33.0	31.0	34.0	30.0	34.0
2	32.15573323095669	34.0	31.0	34.0	30.0	34.0
3	32.16802009697961	34.0	31.0	34.0	30.0	34.0
4	35.74510553418072	37.0	35.0	37.0	33.0	37.0
5	35.62175948722657	37.0	35.0	37.0	33.0	37.0
6	35.618586367771385	37.0	35.0	37.0	33.0	37.0
7	35.808147288827314	37.0	35.0	37.0	35.0	37.0
8	35.64532503192315	37.0	35.0	37.0	35.0	37.0
9	37.34595347983208	39.0	37.0	39.0	35.0	39.0
10	36.99035378362363	39.0	37.0	39.0	33.0	39.0
11	36.93904138742604	39.0	37.0	39.0	33.0	39.0
12	36.750386833473826	39.0	35.0	39.0	33.0	39.0
13	36.64062460878492	39.0	35.0	39.0	33.0	39.0
14	37.731616855423596	40.0	36.0	41.0	33.0	41.0
15	37.89600146888223	40.0	36.0	41.0	33.0	41.0
16	37.96731069362956	40.0	36.0	41.0	33.0	41.0
17	37.913594671963544	40.0	36.0	41.0	33.0	41.0
18	37.88961350036305	40.0	36.0	41.0	33.0	41.0
19	37.83288460093975	40.0	36.0	41.0	33.0	41.0
20	37.74816514909989	40.0	36.0	41.0	33.0	41.0
21	37.63177960089802	39.0	35.0	41.0	33.0	41.0
22	37.59996995468165	39.0	35.0	41.0	33.0	41.0
23	37.53048181006351	39.0	35.0	41.0	33.0	41.0
24	37.50638546474266	39.0	35.0	41.0	33.0	41.0
25	37.3948288668742	39.0	35.0	41.0	33.0	41.0
26	37.35576995301246	39.0	35.0	41.0	33.0	41.0
27	37.30353115949891	39.0	35.0	41.0	32.0	41.0
28	37.17242507448735	39.0	35.0	41.0	32.0	41.0
29	37.103816590023285	39.0	35.0	41.0	32.0	41.0
30	36.995933866915934	39.0	35.0	41.0	32.0	41.0
31	36.875852744556376	39.0	35.0	41.0	31.0	41.0
32	36.76765621479064	39.0	35.0	41.0	31.0	41.0
33	36.67297173236298	39.0	35.0	41.0	31.0	41.0
34	36.56092606347908	39.0	35.0	41.0	31.0	41.0
35	36.46981363556698	39.0	35.0	41.0	30.0	41.0
36	36.34482344202505	39.0	35.0	40.0	30.0	41.0
37	36.224274948046634	38.0	35.0	40.0	30.0	41.0
38	36.12107428704963	38.0	35.0	40.0	30.0	41.0
39	36.06866523673207	38.0	35.0	40.0	30.0	41.0
40	35.93166025421678	38.0	35.0	40.0	29.0	41.0
41	35.80869978884818	38.0	35.0	40.0	29.0	41.0
42	35.70855206603294	38.0	35.0	40.0	29.0	41.0
43	35.57243508959347	38.0	34.0	40.0	29.0	41.0
44	35.43513132307898	38.0	34.0	40.0	28.0	41.0
45	35.358248691776765	37.0	34.0	40.0	28.0	41.0
46	35.18740934242482	37.0	34.0	40.0	27.0	41.0
47	35.07405002545506	37.0	34.0	40.0	27.0	41.0
48	34.98599888164648	37.0	34.0	40.0	27.0	41.0
49	34.922848630016944	37.0	34.0	40.0	27.0	41.0
50	34.791523881855134	36.0	34.0	40.0	26.0	41.0
51	33.169034961066274	35.0	31.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	8.0
12	4.0
13	7.0
14	9.0
15	24.0
16	65.0
17	131.0
18	251.0
19	524.0
20	735.0
21	1146.0
22	1748.0
23	2472.0
24	3266.0
25	4300.0
26	5293.0
27	6482.0
28	7688.0
29	9125.0
30	11704.0
31	14928.0
32	19026.0
33	26316.0
34	45331.0
35	59273.0
36	51194.0
37	73271.0
38	112845.0
39	141881.0
40	45.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.859304450880074	20.092305894724543	24.64584080988825	13.40254884450713
2	30.515026832138474	25.75017317787663	25.919428471277513	17.815371518707384
3	32.26232901292783	25.147764544855157	25.616471511196053	16.973434931020957
4	28.7395154357823	27.232241964963823	24.840634623891038	19.187607975362837
5	24.065465410327246	31.794790475634084	24.027074170206728	20.112669943831946
6	24.190988073677797	37.071249134110616	23.49126599287258	15.246496799339004
7	81.14589505837972	7.032774434772449	7.228736677822383	4.592593829025446
8	82.63630976723223	5.825286473764595	6.583430006927116	4.954973752076048
9	76.88730501840276	7.695440622939601	9.252121950608835	6.165132408048807
10	42.0701224346723	24.421168595965582	16.30108747360602	17.207621495756097
11	33.02080638296096	25.527003229871724	21.390764402974487	20.061425984192823
12	27.589781253390534	23.4029661405954	27.99288927465594	21.014363331358133
13	23.86900241197139	26.491791785943796	29.095719376726564	20.54348642535825
14	21.037731912301055	29.775244326859678	28.420033550605496	20.766990210233768
15	19.831078543469733	25.410827998898338	33.29171500346356	21.46637845416837
16	22.55819193950876	23.761173102763337	30.486984535006968	23.19365042272094
17	21.066441883173788	23.962810572613694	27.393985928775905	27.576761615436617
18	23.599762975821864	23.95463156928367	28.387985211026635	24.057620243867834
19	25.49061501097489	26.25493452624375	24.868009247281318	23.386441215500046
20	25.685241906542366	25.719126348909604	24.40213989434063	24.193491850207398
21	24.64383778866457	27.325716288735507	26.36710371476978	21.663342207830144
22	23.28211719343343	24.47558400587553	24.971164840300787	27.271133960390255
23	22.080471377661308	27.31636885635834	24.66036271375992	25.942797052220435
24	22.98516929702301	26.01841110341432	27.35008638029027	23.646333219272403
25	22.65633997946903	27.043791051502687	25.71979402265083	24.580074946377454
26	21.08046303173954	28.50933491349452	25.085670886921108	25.32453116784483
27	23.844298483546016	26.18399419123845	25.74967242257071	24.222034902644822
28	22.126040110500004	27.117068244602276	27.279479882155584	23.477411762742136
29	22.986504644505462	26.04461729775745	26.538362029394335	24.43051602834275
30	24.49160817566496	27.27514000283761	25.396306095026667	22.83694572647076
31	26.046787237416435	28.923626469925473	21.815237983959136	23.214348308698955
32	26.364266101369566	27.985211026631834	21.978317295253675	23.672205576744922
33	25.989200377235665	26.24792395196087	21.518623924419334	26.24425174638413
34	22.093157178744608	27.74584999040219	24.5103030404193	25.650689790433905
35	24.46924110533388	26.170139961108003	24.814428429547903	24.546190504010216
36	25.641342358056736	27.44589756215625	23.54417913686477	23.36858094292224
37	23.581068111067527	28.43739306787738	25.363923918577186	22.617614902477904
38	23.228870212570627	28.084026740333336	24.13907644029745	24.548026606798587
39	23.560036388218897	26.037940560345184	25.032924661364227	25.36909839007169
40	24.351062853136813	24.024236556806517	27.08068002570544	24.54402056435123
41	20.917383720445002	24.886203356729734	26.66939300111001	27.52701992171525
42	23.128552232951368	25.404485098356687	26.136088600305463	25.330874068386482
43	24.231048498151377	23.490598319131355	26.65420342349711	25.624149759220156
44	23.73897295086756	24.95580834425258	25.671888431717843	25.63333027316202
45	24.21786194176216	24.92509535215617	25.35591183368247	25.5011308723992
46	24.224371760739114	25.53501531476644	26.41150401856133	23.829108905933115
47	22.47890568273813	25.57591033141655	27.168479122676704	24.776704863168614
48	22.26558392241631	24.806249426217878	26.86485448885402	26.063312162511785
49	23.459551490164333	23.98634607199192	28.068670244285133	24.48543219355862
50	23.019554494696166	23.42783698745608	27.670069020772996	25.882539497074752
51	22.10584297982791	24.940284929769067	25.735818192440263	27.21805389796276
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1306.0
1	1435.5
2	1565.0
3	1650.5
4	1736.0
5	1345.5
6	955.0
7	1045.0
8	1135.0
9	1240.5
10	1346.0
11	1359.0
12	1372.0
13	1358.5
14	1345.0
15	1444.5
16	1544.0
17	1507.0
18	1470.0
19	1533.5
20	1597.0
21	1509.5
22	1422.0
23	1752.5
24	2083.0
25	2263.0
26	3559.5
27	4676.0
28	5051.0
29	5426.0
30	6252.0
31	7078.0
32	7836.5
33	8595.0
34	10053.5
35	11512.0
36	12038.0
37	12564.0
38	13892.5
39	15221.0
40	18460.5
41	21700.0
42	25911.0
43	30122.0
44	35155.5
45	40189.0
46	44829.0
47	49469.0
48	54439.5
49	59410.0
50	57271.5
51	55133.0
52	49576.0
53	44019.0
54	39742.0
55	35465.0
56	33517.0
57	31569.0
58	30144.0
59	28719.0
60	25929.0
61	23139.0
62	21989.5
63	20840.0
64	19184.0
65	17528.0
66	16441.0
67	15354.0
68	13060.5
69	10767.0
70	9301.0
71	7835.0
72	7235.0
73	6635.0
74	6123.5
75	4865.5
76	4119.0
77	3176.0
78	2233.0
79	1713.0
80	1193.0
81	1021.5
82	850.0
83	632.0
84	414.0
85	302.5
86	191.0
87	118.0
88	45.0
89	59.0
90	73.0
91	52.0
92	31.0
93	29.0
94	27.0
95	25.0
96	23.0
97	11.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	599095.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.94529991449084
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.29155224177542	20.018081562677033
2	10.175565825493624	5.483673459351386
3	3.750977506931754	3.032136416903557
4	1.8912731482847611	2.0384368880302475
5	1.2041528942236204	1.6223130438878808
6	0.7441434333757857	1.2030700795025688
7	0.5804575405504968	1.0948421762432605
8	0.4573543907506185	0.9858840980787719
9	0.3771076978340666	0.9145152016381901
>10	5.032540835002848	33.31643889189587
>50	1.4109626123549923	25.050209195851714
>100	0.07520990136341589	3.349172505411898
>500	0.0062156943275550325	0.9891590147003385
>1k	0.002486277731022013	0.9020674658272982
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGC	1556	0.25972508533705	Illumina PCR Primer Index 7 (96% over 27bp)
CCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGC	1422	0.23735801500596732	Illumina PCR Primer Index 7 (96% over 27bp)
CTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGCT	1326	0.22133384521653496	Illumina PCR Primer Index 7 (96% over 28bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1082	0.1806057470017276	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	690	0.11517372036154534	No Hit
TCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGC	644	0.1074954723374423	Illumina PCR Primer Index 7 (96% over 27bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCC	636	0.1061601248549896	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	625	0.10432402206661714	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.6691843530658744E-4	0.0	0.0	0.23101511446431702	0.0
2	1.6691843530658744E-4	0.0	0.0	0.957277226483279	0.0
3	1.6691843530658744E-4	0.0	0.0	1.2462130379989818	0.0
4	1.6691843530658744E-4	0.0	0.0	1.6585015732062527	0.0
5	1.6691843530658744E-4	0.0	0.0	2.861315818025522	0.0
6	1.6691843530658744E-4	0.0	0.0	3.8459676678990813	0.0
7	1.6691843530658744E-4	0.0	0.0	4.698753953880436	0.0
8	1.6691843530658744E-4	0.0	0.0	6.076999474206929	0.0
9	1.6691843530658744E-4	0.0	0.0	6.679241188793096	0.0
10	1.6691843530658744E-4	0.0	0.0	7.811448935477679	0.0
11	1.6691843530658744E-4	0.0	0.0	9.154641584389788	0.0
12	1.6691843530658744E-4	0.0	0.0	10.264315342307981	0.0
13	1.6691843530658744E-4	0.0	0.0	10.68745357581018	0.0
14	1.6691843530658744E-4	0.0	0.0	10.859045727305352	0.0
15	1.6691843530658744E-4	0.0	0.0	11.143641659503084	0.0
16	1.6691843530658744E-4	0.0	0.0	11.748554069054157	0.0
17	1.6691843530658744E-4	0.0	0.0	12.498184762016042	0.0
18	1.6691843530658744E-4	0.0	0.0	13.435932531568449	0.0
19	1.6691843530658744E-4	0.0	0.0	13.875261853295386	0.0
20	3.338368706131749E-4	0.0	0.0	14.325440873317254	0.0
21	3.338368706131749E-4	0.0	0.0	14.801659169246948	0.0
22	3.338368706131749E-4	0.0	0.0	15.294903145577914	0.0
23	5.007553059197623E-4	0.0	0.0	15.798162228027275	0.0
24	0.001168429047146112	0.0	0.0	16.20577704704596	0.0
25	0.001168429047146112	0.0	0.0	16.574833707508827	0.0
26	0.001168429047146112	0.0	0.0	16.907835985945468	0.0
27	0.001168429047146112	0.0	0.0	17.266042948113405	0.0
28	0.001168429047146112	0.0	0.0	17.628923626469927	0.0
29	0.001168429047146112	0.0	0.0	17.99213814169706	0.0
30	0.001168429047146112	0.0	0.0	18.40542818751617	0.0
31	0.001168429047146112	0.0	0.0	18.785167627838657	0.0
32	0.001168429047146112	0.0	0.0	19.14587836653619	0.0
33	0.001168429047146112	0.0	0.0	19.507256778974956	0.0
34	0.001168429047146112	0.0	0.0	19.87130588637862	0.0
35	0.0013353474824526995	0.0	0.0	20.25238067418356	0.0
36	0.0013353474824526995	0.0	0.0	20.599070264315344	0.0
37	0.0013353474824526995	0.0	0.0	20.942254567305685	0.0
38	0.0013353474824526995	0.0	0.0	21.27892905131907	0.0
39	0.0013353474824526995	0.0	0.0	21.619776496215124	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCATCG	90	0.0	45.000004	21
CAATAAG	45	3.8380676E-10	45.000004	15
TCGAAGT	35	1.2106284E-7	45.000004	41
AACACGA	45	3.8380676E-10	45.000004	45
AAGTACG	25	3.8885613E-5	45.0	1
TACCCGT	25	3.8885613E-5	45.0	35
CCCTCGA	20	7.030579E-4	45.0	14
GGCGTAT	25	3.8885613E-5	45.0	8
ATTACCG	20	7.030579E-4	45.0	1
TTCGTCA	20	7.030579E-4	45.0	24
CGCGATG	25	3.8885613E-5	45.0	45
TCTACGG	20	7.030579E-4	45.0	2
CGCTATA	25	3.8885613E-5	45.0	20
GTATCGA	20	7.030579E-4	45.0	37
AGCTCGA	20	7.030579E-4	45.0	10
CGCCGTT	20	7.030579E-4	45.0	26
CGACTCA	20	7.030579E-4	45.0	10
TTCGCAT	25	3.8885613E-5	45.0	35
CAATCCA	20	7.030579E-4	45.0	36
TCGACAC	20	7.030579E-4	45.0	34
>>END_MODULE