FastQCFastQC Report
Sat 18 Jun 2016
SRR3554038_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554038_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences878164
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCTGC14960.17035542336055678Illumina PCR Primer Index 9 (95% over 23bp)
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC9640.10977448403715023No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA9440.10749700511521766No Hit
CTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCTGCT9010.10260042543306261RNA PCR Primer, Index 17 (96% over 25bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT8950.10191718175648284No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTACG207.032375E-445.0000041
TAATGCG453.8380676E-1045.0000041
TGCGTAG453.8380676E-1045.0000041
TAGTACG351.2114833E-745.01
CATACGA1350.041.66666818
CGACGGT501.0804797E-940.528
TATACGG501.0804797E-940.52
AATGCGG1800.040.0000042
ACACGAC1250.039.626
CCCTCGT1650.039.54545214
GTAGACG356.246608E-638.5714261
TCGTAAG356.246608E-638.5714261
GTCGATG659.094947E-1238.076921
TATTAGT1300.038.0769230
TTAACGG659.094947E-1238.076922
GACAAAC1550.037.7419412
AAGTACG301.1397024E-437.5000041
TACCCGA301.1397024E-437.50000422
TGATTCG301.1397024E-437.50000415
ACGAGAT301.1397024E-437.5000043
Produced by FastQC (version 0.11.5)