##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554038_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	878164
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.06716626962618	33.0	31.0	34.0	30.0	34.0
2	32.180200964740074	34.0	31.0	34.0	30.0	34.0
3	32.203925462669844	34.0	31.0	34.0	30.0	34.0
4	35.76730200736992	37.0	35.0	37.0	35.0	37.0
5	35.64564136083921	37.0	35.0	37.0	33.0	37.0
6	35.63285673291094	37.0	35.0	37.0	33.0	37.0
7	35.826998146132155	37.0	35.0	37.0	35.0	37.0
8	35.67051712436401	37.0	35.0	37.0	35.0	37.0
9	37.35008608870325	39.0	37.0	39.0	35.0	39.0
10	36.969103720945064	39.0	37.0	39.0	33.0	39.0
11	36.94304025216247	39.0	37.0	39.0	33.0	39.0
12	36.84264328758638	39.0	35.0	39.0	33.0	39.0
13	36.81822301984595	39.0	37.0	39.0	33.0	39.0
14	37.93759821627851	40.0	37.0	41.0	33.0	41.0
15	38.05811784586933	40.0	37.0	41.0	33.0	41.0
16	38.105419944338415	40.0	37.0	41.0	33.0	41.0
17	38.05606242114229	40.0	37.0	41.0	33.0	41.0
18	38.030634368978916	40.0	37.0	41.0	33.0	41.0
19	37.990284274919034	40.0	37.0	41.0	33.0	41.0
20	37.9463152668522	40.0	36.0	41.0	33.0	41.0
21	37.82277797768982	40.0	36.0	41.0	33.0	41.0
22	37.803334001393814	40.0	36.0	41.0	33.0	41.0
23	37.7601347812026	40.0	36.0	41.0	33.0	41.0
24	37.73805234557554	40.0	36.0	41.0	33.0	41.0
25	37.60948182799568	40.0	36.0	41.0	33.0	41.0
26	37.560034344382146	40.0	36.0	41.0	33.0	41.0
27	37.50494896169736	40.0	36.0	41.0	33.0	41.0
28	37.385307300230934	39.0	36.0	41.0	32.0	41.0
29	37.33860190123941	39.0	35.0	41.0	32.0	41.0
30	37.23546740699915	39.0	35.0	41.0	32.0	41.0
31	37.17634860914362	39.0	35.0	41.0	32.0	41.0
32	37.04551200003644	39.0	35.0	41.0	31.0	41.0
33	36.971948292118554	39.0	35.0	41.0	31.0	41.0
34	36.88865633298564	39.0	35.0	41.0	31.0	41.0
35	36.8068470126309	39.0	35.0	41.0	31.0	41.0
36	36.70878218647086	39.0	35.0	41.0	31.0	41.0
37	36.630428940380156	39.0	35.0	41.0	31.0	41.0
38	36.51429915141135	39.0	35.0	41.0	30.0	41.0
39	36.44001803763306	39.0	35.0	41.0	30.0	41.0
40	36.298970351779396	39.0	35.0	40.0	30.0	41.0
41	36.17477828742695	38.0	35.0	40.0	30.0	41.0
42	36.08993764262712	38.0	35.0	40.0	29.0	41.0
43	35.991461731521674	38.0	35.0	40.0	29.0	41.0
44	35.872387162306815	38.0	35.0	40.0	29.0	41.0
45	35.794620367038505	38.0	34.0	40.0	29.0	41.0
46	35.65214242442186	38.0	34.0	40.0	28.0	41.0
47	35.559811151447796	38.0	34.0	40.0	28.0	41.0
48	35.42827649505104	37.0	34.0	40.0	28.0	41.0
49	35.31122432711885	37.0	34.0	40.0	28.0	41.0
50	35.155578001375595	37.0	34.0	40.0	27.0	41.0
51	33.59323087714823	35.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	5.0
11	10.0
12	8.0
13	19.0
14	30.0
15	55.0
16	107.0
17	151.0
18	348.0
19	547.0
20	959.0
21	1391.0
22	2063.0
23	2795.0
24	4107.0
25	5272.0
26	6855.0
27	8467.0
28	10412.0
29	12915.0
30	16351.0
31	20926.0
32	26818.0
33	36211.0
34	60337.0
35	73553.0
36	76112.0
37	109577.0
38	173526.0
39	228161.0
40	72.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.83657266751996	17.670503459490483	22.719674229415006	15.77324964357455
2	28.406083601696263	25.394117727440435	27.726142269553293	18.473656401310006
3	33.20461781626211	24.942038161436816	25.634733375542613	16.218610646758464
4	28.442295516554992	26.712322527455008	25.407554853079834	19.437827102910163
5	24.811083123425693	30.867013450790516	24.402503404830988	19.919400020952807
6	24.75107155383277	36.70521679321858	23.651732478215916	14.89197917473274
7	82.33564573359874	6.470317617210453	6.8391553286174345	4.354881320573378
8	84.38628775490683	5.137992447879895	6.148737593433573	4.326982203779704
9	78.21192852360151	7.500421333600557	8.703499574111442	5.584150568686487
10	41.67114570854647	25.835151520672678	15.787142264998339	16.70656050578252
11	31.87969445342783	26.666203579285874	21.364574270865123	20.089527696421168
12	26.964894939897334	24.075571305587566	28.256908732309682	20.70262502220542
13	24.985651882791828	25.1114825932286	29.1176818908541	20.785183633125477
14	22.01297251993933	28.155788668175873	27.89581444923727	21.935424362647524
15	21.52980536665133	26.299529472854726	31.043973563024675	21.126691597469264
16	24.263463316647005	24.09196915382548	30.117039641798115	21.5275278877294
17	23.903735520927754	24.091513658041094	27.032422189932632	24.97232863109852
18	24.370504825977836	25.34355769537353	27.226463394081286	23.05947408456735
19	25.273980714308493	27.133997749850824	25.431468381760126	22.16055315408056
20	26.56166729676917	25.23549132052783	25.719341717492405	22.483499665210598
21	26.136917477828742	26.48696598812978	26.102299798215366	21.27381673582611
22	24.602807676014958	24.32336101229383	25.451737944165327	25.622093367525885
23	23.693865838271666	26.83724224632301	25.07959788832154	24.38929402708378
24	23.64512778934231	25.048168679198874	27.506137805694607	23.80056572576421
25	23.972059888585733	26.308297766704168	25.821031151356692	23.898611193353407
26	23.490828592381376	26.642403924551676	25.86669460374144	24.0000728793255
27	24.452380193221313	25.45458479281774	26.14056144410383	23.952473569857112
28	23.043076236329433	26.68522052828401	27.009989022551594	23.26171421283496
29	24.149133874765987	26.583758842311916	25.23514969868954	24.031957584232558
30	24.914822288319723	25.482939405395804	25.532588445893932	24.069649860390545
31	26.751039669127863	26.598904077142766	22.686309163208694	23.963747090520677
32	25.97544422226372	26.758099853785854	24.231692485686047	23.03476343826438
33	25.376239517903258	26.745573719715228	23.4023485362643	24.475838226117215
34	24.642094187418294	26.983000897326697	24.772479855698936	23.602425059556072
35	24.321652903102382	26.20228112288821	25.142228558674688	24.33383741533472
36	26.13805621728971	27.57127370286188	24.108822497847783	22.18184758200063
37	24.144578916922125	29.126677932595733	24.428694412433213	22.30004873804893
38	24.396012589903478	28.749299675231505	24.599505331578158	22.25518240328686
39	24.884987314442405	27.228626999057127	24.629909675185957	23.256476011314515
40	25.165686591570598	25.45287668362629	27.10473214570399	22.27670457909912
41	22.255296277232954	26.416591889442063	26.41966648598667	24.908445347338308
42	23.092383654989273	26.890421379150137	26.146369015354765	23.87082595050583
43	24.20014940261728	24.75437389826957	26.60300353920225	24.442473159910904
44	24.452721815059604	25.30871226786796	26.051398144310173	24.187167772762265
45	25.190055616035274	24.98508251306134	25.218751850451625	24.60611002045176
46	24.6081597514815	26.081460866079688	25.550922151215488	23.759457231223323
47	23.689652502266092	25.74348299406489	26.96284520886759	23.604019294801425
48	22.49762003452658	25.73699217913738	27.214620503687236	24.5507672826488
49	23.50210211304494	26.251246919709757	26.524658264287766	23.721992702957532
50	22.13310953307127	25.729476498695004	27.827603955525394	24.309810012708333
51	22.759530110548827	25.889127771122478	25.910308325096455	25.441033793232243
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1466.0
1	1655.0
2	1844.0
3	1903.0
4	1962.0
5	1574.0
6	1186.0
7	1227.5
8	1269.0
9	1376.0
10	1483.0
11	1519.5
12	1556.0
13	1574.0
14	1592.0
15	1619.5
16	1647.0
17	1650.0
18	1653.0
19	1724.5
20	1796.0
21	2089.0
22	2382.0
23	2902.0
24	3422.0
25	4929.5
26	6852.5
27	7268.0
28	8871.0
29	10474.0
30	12447.5
31	14421.0
32	15448.5
33	16476.0
34	17903.0
35	19330.0
36	20868.0
37	22406.0
38	25331.5
39	28257.0
40	30480.5
41	32704.0
42	37846.5
43	42989.0
44	46543.0
45	50097.0
46	56423.5
47	62750.0
48	69377.5
49	76005.0
50	74472.0
51	72939.0
52	66047.0
53	59155.0
54	55036.0
55	50917.0
56	49821.5
57	48726.0
58	48249.5
59	47773.0
60	45089.5
61	42406.0
62	40510.0
63	38614.0
64	34097.5
65	29581.0
66	25557.5
67	21534.0
68	19245.5
69	16957.0
70	14968.5
71	12980.0
72	11093.0
73	9206.0
74	7606.0
75	4877.0
76	3748.0
77	3000.5
78	2253.0
79	1746.5
80	1240.0
81	955.5
82	671.0
83	469.0
84	267.0
85	206.0
86	145.0
87	92.0
88	39.0
89	55.0
90	71.0
91	42.5
92	14.0
93	17.5
94	21.0
95	21.5
96	22.0
97	14.5
98	7.0
99	3.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	878164.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.332791036111047
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.36801948731912	19.319847258928974
2	9.088735571500656	4.786635491735922
3	3.261418467763798	2.5764675297881268
4	1.7827113294088512	1.8777505966012402
5	1.1448436830885662	1.5073464737891478
6	0.9052458681927408	1.4302590170053424
7	0.7532062149317503	1.3883815305428548
8	0.5662899576864129	1.1929596097283584
9	0.5323982702895085	1.2617579159568537
>10	7.845683860217949	49.697880386963334
>50	0.6851431170692975	10.88323744786539
>100	0.05937040285486868	2.612684503298502
>500	0.006500409071700949	1.2940747878316232
>1k	4.333606047800633E-4	0.17071744996438346
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCTGC	1496	0.17035542336055678	Illumina PCR Primer Index 9 (95% over 23bp)
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	964	0.10977448403715023	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	944	0.10749700511521766	No Hit
CTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCTGCT	901	0.10260042543306261	RNA PCR Primer, Index 17 (96% over 25bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	895	0.10191718175648284	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1387394609662888E-4	0.0	0.0	0.10669988749254125	0.0
2	1.1387394609662888E-4	0.0	0.0	0.4985401360110412	0.0
3	1.1387394609662888E-4	0.0	0.0	0.6832436765797733	0.0
4	1.1387394609662888E-4	0.0	0.0	0.9550607859124264	0.0
5	1.1387394609662888E-4	0.0	0.0	1.7522922825349252	0.0
6	1.1387394609662888E-4	0.0	0.0	2.4996469907671006	0.0
7	1.1387394609662888E-4	0.0	0.0	3.0973713338283053	0.0
8	1.1387394609662888E-4	0.0	0.0	4.096615210826224	0.0
9	1.1387394609662888E-4	0.0	0.0	4.5236425086885825	0.0
10	1.1387394609662888E-4	0.0	0.0	5.309486610701418	0.0
11	1.1387394609662888E-4	0.0	0.0	6.17686445811944	0.0
12	1.1387394609662888E-4	0.0	0.0	6.892562209336752	0.0
13	1.1387394609662888E-4	0.0	0.0	7.174741847764199	0.0
14	1.1387394609662888E-4	0.0	0.0	7.298864449009525	0.0
15	1.1387394609662888E-4	0.0	0.0	7.500193585708364	0.0
16	1.1387394609662888E-4	0.0	0.0	7.941796748671091	0.0
17	1.1387394609662888E-4	0.0	0.0	8.513216210183975	0.0
18	1.1387394609662888E-4	0.0	0.0	9.149999316756324	0.0
19	1.1387394609662888E-4	0.0	0.0	9.4615584332767	0.0
20	1.1387394609662888E-4	0.0	0.0	9.779494490778488	0.0
21	1.1387394609662888E-4	0.0	0.0	10.16723527723751	0.0
22	1.1387394609662888E-4	0.0	0.0	10.588341129902842	0.0
23	1.1387394609662888E-4	0.0	0.0	11.016848789064458	0.0
24	1.1387394609662888E-4	0.0	0.0	11.365758559904528	0.0
25	1.1387394609662888E-4	0.0	0.0	11.669232626252043	0.0
26	1.1387394609662888E-4	0.0	0.0	11.948451542080978	0.0
27	1.1387394609662888E-4	0.0	0.0	12.227898205802106	0.0
28	1.1387394609662888E-4	0.0	0.0	12.527272810090142	0.0
29	1.1387394609662888E-4	0.0	0.0	12.825736422809406	0.0
30	1.1387394609662888E-4	0.0	0.0	13.176354302840927	0.0
31	2.2774789219325776E-4	0.0	0.0	13.489279906714463	0.0
32	2.2774789219325776E-4	0.0	0.0	13.807671460000638	0.0
33	2.2774789219325776E-4	0.0	0.0	14.122532920957816	0.0
34	2.2774789219325776E-4	0.0	0.0	14.41997166816221	0.0
35	3.4162183828988663E-4	0.0	0.0	14.749864490004144	0.0
36	3.4162183828988663E-4	0.0	0.0	15.060512614955748	0.0
37	3.4162183828988663E-4	0.0	0.0	15.363303437626685	0.0
38	3.4162183828988663E-4	0.0	0.0	15.66951047868052	0.0
39	3.4162183828988663E-4	0.0	0.0	15.986307796721341	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAGTACG	20	7.032375E-4	45.000004	1
TAATGCG	45	3.8380676E-10	45.000004	1
TGCGTAG	45	3.8380676E-10	45.000004	1
TAGTACG	35	1.2114833E-7	45.0	1
CATACGA	135	0.0	41.666668	18
CGACGGT	50	1.0804797E-9	40.5	28
TATACGG	50	1.0804797E-9	40.5	2
AATGCGG	180	0.0	40.000004	2
ACACGAC	125	0.0	39.6	26
CCCTCGT	165	0.0	39.545452	14
GTAGACG	35	6.246608E-6	38.571426	1
TCGTAAG	35	6.246608E-6	38.571426	1
GTCGATG	65	9.094947E-12	38.07692	1
TATTAGT	130	0.0	38.07692	30
TTAACGG	65	9.094947E-12	38.07692	2
GACAAAC	155	0.0	37.74194	12
AAGTACG	30	1.1397024E-4	37.500004	1
TACCCGA	30	1.1397024E-4	37.500004	22
TGATTCG	30	1.1397024E-4	37.500004	15
ACGAGAT	30	1.1397024E-4	37.500004	3
>>END_MODULE