##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554033_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1576702
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.955101851840105	33.0	31.0	34.0	30.0	34.0
2	32.05698223253348	33.0	31.0	34.0	30.0	34.0
3	32.07996311287739	33.0	31.0	34.0	30.0	34.0
4	35.677614412869396	37.0	35.0	37.0	33.0	37.0
5	35.55478016771717	37.0	35.0	37.0	33.0	37.0
6	35.54760823541798	37.0	35.0	37.0	33.0	37.0
7	35.8545603417767	37.0	35.0	37.0	35.0	37.0
8	35.72876612067468	37.0	35.0	37.0	35.0	37.0
9	37.404791774222396	39.0	37.0	39.0	35.0	39.0
10	36.85445696142962	39.0	37.0	39.0	32.0	39.0
11	36.865248474347084	39.0	37.0	39.0	33.0	39.0
12	36.862539655559516	39.0	37.0	39.0	33.0	39.0
13	36.872154662073115	39.0	37.0	39.0	33.0	39.0
14	37.987615922349306	40.0	37.0	41.0	33.0	41.0
15	38.09321101894968	40.0	37.0	41.0	33.0	41.0
16	38.14710008612915	40.0	37.0	41.0	33.0	41.0
17	38.097129958609806	40.0	37.0	41.0	33.0	41.0
18	38.04917606497613	40.0	37.0	41.0	33.0	41.0
19	38.01213165201795	40.0	37.0	41.0	33.0	41.0
20	37.97285282824529	40.0	37.0	41.0	33.0	41.0
21	37.88778792695132	40.0	37.0	41.0	33.0	41.0
22	37.90061089540065	40.0	37.0	41.0	33.0	41.0
23	37.83911037088809	40.0	37.0	41.0	33.0	41.0
24	37.81808610631559	40.0	36.0	41.0	33.0	41.0
25	37.740998616098665	40.0	36.0	41.0	33.0	41.0
26	37.704433050760386	40.0	36.0	41.0	33.0	41.0
27	37.676553337282506	40.0	36.0	41.0	33.0	41.0
28	37.599869854925025	40.0	36.0	41.0	33.0	41.0
29	37.57786696534919	40.0	36.0	41.0	33.0	41.0
30	37.481049684721654	39.0	36.0	41.0	32.0	41.0
31	37.42560737539497	39.0	36.0	41.0	32.0	41.0
32	37.32277247063808	39.0	36.0	41.0	32.0	41.0
33	37.2550386820084	39.0	36.0	41.0	32.0	41.0
34	37.20339480764279	39.0	36.0	41.0	32.0	41.0
35	37.1489285863784	39.0	35.0	41.0	31.0	41.0
36	37.09437991453046	39.0	35.0	41.0	31.0	41.0
37	37.048145432681636	39.0	35.0	41.0	31.0	41.0
38	36.957003923379304	39.0	35.0	41.0	31.0	41.0
39	36.90410489743782	39.0	35.0	41.0	31.0	41.0
40	36.81919855495839	39.0	35.0	41.0	31.0	41.0
41	36.75081974907116	39.0	35.0	41.0	31.0	41.0
42	36.65214606184301	39.0	35.0	41.0	30.0	41.0
43	36.56766338851603	39.0	35.0	40.0	30.0	41.0
44	36.47650602333225	39.0	35.0	40.0	30.0	41.0
45	36.452293458117005	39.0	35.0	40.0	30.0	41.0
46	36.35066613729164	39.0	35.0	40.0	30.0	41.0
47	36.23890563974676	38.0	35.0	40.0	30.0	41.0
48	36.16722563934085	38.0	35.0	40.0	30.0	41.0
49	36.09562491834221	38.0	35.0	40.0	29.0	41.0
50	35.988630064527094	38.0	35.0	40.0	29.0	41.0
51	34.4485666917401	37.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	10.0
11	3.0
12	12.0
13	18.0
14	34.0
15	56.0
16	101.0
17	209.0
18	328.0
19	666.0
20	1172.0
21	1878.0
22	2956.0
23	4069.0
24	6047.0
25	8359.0
26	10504.0
27	13202.0
28	16303.0
29	20538.0
30	26894.0
31	34763.0
32	45834.0
33	62132.0
34	97022.0
35	123032.0
36	132386.0
37	192048.0
38	327037.0
39	448947.0
40	140.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.80523396304438	18.701885327728384	23.70086420896276	13.792016500264475
2	28.670287727167214	26.852188936146465	26.711008167681655	17.766515169004666
3	31.206911642149244	26.575218398911144	26.072079568618545	16.145790390321064
4	27.508115040128068	27.0499434896385	26.486869427450465	18.955072042782973
5	24.704604928515344	31.374540020879028	25.092186094772508	18.828668955833123
6	23.83836641293028	36.64471789849952	24.971364278094402	14.54555141047579
7	82.91198971016716	5.361634601846132	7.7645617244095595	3.9618139635771374
8	84.66577704601123	4.767863553163502	6.312416677342961	4.253942723482307
9	78.5631653920652	7.2543828827514645	8.263387754946718	5.919063970236608
10	36.62727642890032	29.650878859797224	17.716347160084783	16.00549755121767
11	28.319492205882913	27.57978362429933	24.553656937074983	19.547067232742776
12	24.32577620882069	24.330279279153576	30.252451002155134	21.091493509870602
13	23.65234521171407	26.26044744028992	31.49079534369843	18.596412004297576
14	21.41964683243885	29.568428276237363	28.737136123376516	20.27478876794727
15	20.356034304516644	28.2828968314875	32.32811273151172	19.032956132484134
16	22.91904240623783	26.690458945317502	30.0347814615571	20.355717186887567
17	23.68646706860269	26.535388424699153	27.399597387458126	22.378547119240032
18	23.955446241585285	25.777984679413102	29.27147932837023	20.995089750631383
19	23.21028323678159	28.658364104313943	26.74170515417625	21.389647504728224
20	24.648982496375346	27.49384474681963	27.25112291352456	20.60604984328047
21	23.68551571571546	28.39135106063162	28.183448743009144	19.739684480643774
22	22.788263096006727	25.757054915894063	27.38589790588202	24.068784082217185
23	21.37341108211951	28.856245504857608	27.254928325073475	22.515415087949403
24	22.010246704830717	26.450781441261572	29.322091301970822	22.21688055193689
25	21.626597797174103	29.213256531671806	26.94129898991693	22.218846681237164
26	21.033651254327072	29.138353347683964	27.68830127696927	22.139694121019698
27	22.097644323404168	28.113429170509075	27.0536854776616	22.735241028425158
28	20.91803016676582	28.428580670285193	28.39046313127021	22.26292603167878
29	21.728836520788327	28.196767683430352	27.144698237206523	22.929697558574798
30	22.820038282440184	27.709167616962493	26.845339195358413	22.62545490523891
31	23.223221636047903	28.31498913555003	25.17121180793834	23.290577420463727
32	22.44894723289499	29.41494334376439	26.045378264250314	22.090731159090303
33	22.39231002434195	27.543124826378097	26.03675266473944	24.027812484540515
34	21.462267441786718	27.887831689184132	27.252518231092495	23.397382637936655
35	20.99775353871562	27.467714254183733	27.142858954957884	24.391673252142766
36	23.178381203296503	28.477226514585507	26.683545781003637	21.66084650111435
37	22.604969106400574	27.93394059245184	26.955696130276998	22.50539417087059
38	21.882955688519452	28.334904122656024	27.52644443908868	22.25569574973584
39	22.697504030565067	26.973010752824568	27.12541748535868	23.204067731251687
40	22.210791893458627	26.137215529630836	29.37752346353337	22.27446911337716
41	21.137538989612494	26.20767906681161	27.676377654115996	24.9784042894599
42	22.61873201150249	26.62405451378891	27.548515826072396	23.208697648636203
43	22.907182206910374	26.440316559502048	27.263680771635983	23.388820461951592
44	22.450659668092005	26.43289600698166	27.075756864645317	24.040687460281017
45	21.75407908406281	25.863479592212098	27.389259352750233	24.993181970974856
46	22.697123489410174	26.791365774889613	26.78172539896569	23.729785336734526
47	21.268191452791967	26.340170812239727	29.12820558355352	23.26343215141479
48	21.80050510495959	26.228164865649944	27.92144615786623	24.049883871524234
49	22.72769362885314	24.749191667163483	28.845082964314116	23.678031739669258
50	21.150984777085334	25.103094941212735	29.094210573716527	24.651709707985404
51	20.96731024632429	24.614289827754387	27.427884279971735	26.990515645949582
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2276.0
1	2194.0
2	2112.0
3	2689.0
4	3266.0
5	2538.0
6	1810.0
7	1917.5
8	2025.0
9	2220.0
10	2415.0
11	2471.0
12	2527.0
13	2589.0
14	2651.0
15	2714.0
16	2777.0
17	2947.5
18	3118.0
19	3276.5
20	3435.0
21	3635.5
22	3836.0
23	4622.0
24	5408.0
25	7392.5
26	10972.5
27	12568.0
28	14915.0
29	17262.0
30	19858.5
31	22455.0
32	26798.5
33	31142.0
34	36846.5
35	42551.0
36	47657.0
37	52763.0
38	58194.0
39	63625.0
40	71385.0
41	79145.0
42	88260.0
43	97375.0
44	107339.5
45	117304.0
46	139286.0
47	161268.0
48	166757.0
49	172246.0
50	165769.0
51	159292.0
52	140764.0
53	122236.0
54	106651.5
55	91067.0
56	82185.5
57	73304.0
58	65194.0
59	57084.0
60	52589.0
61	48094.0
62	42505.5
63	36917.0
64	31087.5
65	25258.0
66	20346.0
67	15434.0
68	13096.5
69	10759.0
70	9071.5
71	7384.0
72	5970.0
73	4556.0
74	3970.0
75	2845.5
76	2307.0
77	1705.5
78	1104.0
79	1060.5
80	1017.0
81	672.5
82	328.0
83	311.0
84	294.0
85	184.5
86	75.0
87	50.5
88	26.0
89	20.5
90	15.0
91	14.5
92	14.0
93	13.0
94	12.0
95	7.5
96	3.0
97	1.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1576702.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.45511813412796
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.33121194218465	21.30524392541696
2	10.946566814986511	6.448648373971051
3	4.118845748641711	3.639632643074769
4	2.1155003938794876	2.4924925605805814
5	1.319375094827496	1.9431174640685087
6	0.9487983868023451	1.6768181141239864
7	0.7045527653793532	1.452687945518277
8	0.5934587486071506	1.3984318038364296
9	0.5020478171091177	1.330908998573712
>10	5.535700705695076	36.817428246909394
>50	0.8034186446232131	15.417203760731867
>100	0.07489499184565834	3.839211868186628
>500	0.003246891587436722	0.659325443957259
>1k	0.0023810538307869294	1.578848851050537
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTC	4271	0.2708818787570511	No Hit
GCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTCTGC	3925	0.24893733882496502	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCG	3640	0.23086163396761086	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGC	2820	0.17885434279908313	No Hit
CCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTCTGC	2164	0.13724850986426096	No Hit
CTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTCTGCT	1895	0.12018758141995127	TruSeq Adapter, Index 13 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2684705163055544E-4	0.0	0.0	0.12411984002049849	0.0
2	1.2684705163055544E-4	0.0	0.0	0.6808515496270062	0.0
3	1.2684705163055544E-4	0.0	0.0	0.973107156583806	0.0
4	1.2684705163055544E-4	0.0	0.0	1.4317860952798944	0.0
5	1.2684705163055544E-4	0.0	0.0	2.771608078127636	0.0
6	1.2684705163055544E-4	0.0	0.0	3.839723676382728	0.0
7	1.2684705163055544E-4	0.0	0.0	4.692389557443321	0.0
8	1.2684705163055544E-4	0.0	0.0	6.016609352940505	0.0
9	1.2684705163055544E-4	0.0	0.0	6.560910051487218	0.0
10	1.2684705163055544E-4	0.0	0.0	7.867307836230308	0.0
11	1.2684705163055544E-4	0.0	0.0	9.107301189444803	0.0
12	1.2684705163055544E-4	0.0	0.0	10.33543434333184	0.0
13	1.2684705163055544E-4	0.0	0.0	10.798743199412444	0.0
14	1.2684705163055544E-4	0.0	0.0	11.000049470350136	0.0
15	2.5369410326111087E-4	0.0	0.0	11.327758828237675	0.0
16	2.5369410326111087E-4	0.0	0.0	12.012352365887784	0.0
17	2.5369410326111087E-4	0.0	0.0	12.867618611506803	0.0
18	2.5369410326111087E-4	0.0	0.0	13.842438203287623	0.0
19	2.5369410326111087E-4	0.0	0.0	14.432403840421335	0.0
20	2.5369410326111087E-4	0.0	0.0	14.982349232765609	0.0
21	2.5369410326111087E-4	0.0	0.0	15.649057336135808	0.0
22	2.5369410326111087E-4	0.0	0.0	16.37563724787563	0.0
23	2.5369410326111087E-4	0.0	0.0	17.121180793834217	0.0
24	2.5369410326111087E-4	0.0	0.0	17.69884226695977	0.0
25	2.5369410326111087E-4	0.0	0.0	18.209401649772754	0.0
26	2.5369410326111087E-4	0.0	0.0	18.687551610894133	0.0
27	2.5369410326111087E-4	0.0	0.0	19.18935854714461	0.002156399877719442
28	3.171176290763886E-4	0.0	0.0	19.707592176581244	0.002156399877719442
29	3.171176290763886E-4	0.0	0.0	20.23077284103147	0.002156399877719442
30	3.171176290763886E-4	0.0	0.0	20.85771439371549	0.002156399877719442
31	3.8054115489166625E-4	0.0	0.0	21.386286057860016	0.002156399877719442
32	3.8054115489166625E-4	0.0	0.0	21.929571979993682	0.002156399877719442
33	3.8054115489166625E-4	0.0	0.0	22.4493911975757	0.002156399877719442
34	3.8054115489166625E-4	0.0	0.0	22.961409321482435	0.002156399877719442
35	3.8054115489166625E-4	0.0	0.0	23.50355362015143	0.002156399877719442
36	3.8054115489166625E-4	0.0	0.0	24.034535378276935	0.002156399877719442
37	3.8054115489166625E-4	0.0	0.0	24.55156396072308	0.002156399877719442
38	3.8054115489166625E-4	0.0	0.0	25.07613994274124	0.002156399877719442
39	4.4396468070694397E-4	0.0	0.0	25.60642404208278	0.002156399877719442
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGCGC	20	7.0340856E-4	45.0	39
GAACGTA	20	7.0340856E-4	45.0	9
CTCGAAG	20	7.0340856E-4	45.0	1
TACTACG	20	7.0340856E-4	45.0	1
CGCATCG	50	1.0822987E-9	40.5	21
TATAGCG	45	1.9290383E-8	40.0	1
TAATGCG	115	0.0	39.130436	1
TCGCGAG	35	6.2497693E-6	38.571426	1
CAATTCG	65	9.094947E-12	38.076927	4
GCGCGAC	160	0.0	37.96875	9
AAGGGCG	540	0.0	37.916664	5
CTATGCG	60	1.5643309E-10	37.499996	1
GTCGAAC	30	1.1401145E-4	37.499996	9
GTACGAC	30	1.1401145E-4	37.499996	13
CATCGCC	55	2.750312E-9	36.81818	23
CGCCAGT	55	2.750312E-9	36.81818	26
CGTTTTT	1570	0.0	36.687897	1
GCGAGAC	345	0.0	36.521736	21
CGTAAGG	265	0.0	36.50943	2
CGCATGG	155	0.0	36.290325	2
>>END_MODULE