FastQCFastQC Report
Sat 18 Jun 2016
SRR3554029_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554029_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1479337
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCTGC28810.19474940463193985TruSeq Adapter, Index 16 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCTGC28550.1929918605429324TruSeq Adapter, Index 16 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCTGCT19800.1338437421628743TruSeq Adapter, Index 13 (95% over 22bp)
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT17700.11964819375166037No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA15240.1030191227556669No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT14810.1001124152238469No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATGCG302.1659253E-645.0000041
CTTACGT302.1659253E-645.00000422
TATACGG650.041.5384642
CGTAAGG2300.041.0869562
TTAACGG1050.040.7142872
TACGGGT1500.039.04
TACGAAT2900.038.79310212
GCTACGA2900.038.79310210
GATTACG659.094947E-1238.0769271
CTTGACG950.037.8947371
CGAATAT3050.037.6229514
ACACGAT301.14008006E-437.50000416
CACAACG2800.036.96428312
CGGTCTA1050.036.4285731
CGAGGGT2850.036.315794
ATACTAT4800.036.09375445
TATTAGG2500.036.0000042
AGATACG751.8189894E-1236.01
CCGTAGT250.002107303836.027
GTTCGGA250.002107303836.03
Produced by FastQC (version 0.11.5)