##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554029_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1479337
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.06336554821518	33.0	31.0	34.0	30.0	34.0
2	32.17209871719561	34.0	31.0	34.0	30.0	34.0
3	32.20404951677677	34.0	31.0	34.0	30.0	34.0
4	35.77210331384938	37.0	35.0	37.0	35.0	37.0
5	35.64570817873142	37.0	35.0	37.0	33.0	37.0
6	35.63416854983009	37.0	35.0	37.0	33.0	37.0
7	35.86023130632168	37.0	35.0	37.0	35.0	37.0
8	35.716204624098495	37.0	35.0	37.0	35.0	37.0
9	37.388601109821494	39.0	37.0	39.0	35.0	39.0
10	36.982097385517974	39.0	37.0	39.0	33.0	39.0
11	36.95840907109063	39.0	37.0	39.0	33.0	39.0
12	36.88469632004067	39.0	37.0	39.0	33.0	39.0
13	36.86886355171269	39.0	37.0	39.0	33.0	39.0
14	38.005223961815325	40.0	37.0	41.0	33.0	41.0
15	38.11238953666406	40.0	37.0	41.0	33.0	41.0
16	38.1743700049414	40.0	37.0	41.0	33.0	41.0
17	38.12822095303504	40.0	37.0	41.0	33.0	41.0
18	38.08343805366864	40.0	37.0	41.0	33.0	41.0
19	38.06649735658609	40.0	37.0	41.0	34.0	41.0
20	38.016501311060296	40.0	37.0	41.0	33.0	41.0
21	37.90723276711121	40.0	36.0	41.0	33.0	41.0
22	37.902381269447055	40.0	36.0	41.0	33.0	41.0
23	37.868035478055376	40.0	36.0	41.0	33.0	41.0
24	37.85307877785792	40.0	36.0	41.0	33.0	41.0
25	37.73647924712219	40.0	36.0	41.0	33.0	41.0
26	37.7038923517765	40.0	36.0	41.0	33.0	41.0
27	37.649334803361235	40.0	36.0	41.0	33.0	41.0
28	37.56055787153299	40.0	36.0	41.0	33.0	41.0
29	37.5253170846129	40.0	36.0	41.0	33.0	41.0
30	37.39863668656972	39.0	36.0	41.0	32.0	41.0
31	37.32699310569532	39.0	35.0	41.0	32.0	41.0
32	37.24990113814499	39.0	35.0	41.0	32.0	41.0
33	37.16351041040682	39.0	35.0	41.0	32.0	41.0
34	37.1071709826767	39.0	35.0	41.0	31.0	41.0
35	37.04129485032822	39.0	35.0	41.0	31.0	41.0
36	36.95979550298546	39.0	35.0	41.0	31.0	41.0
37	36.90955880911516	39.0	35.0	41.0	31.0	41.0
38	36.829080865279515	39.0	35.0	41.0	31.0	41.0
39	36.78775356798349	39.0	35.0	41.0	31.0	41.0
40	36.68968733966635	39.0	35.0	41.0	31.0	41.0
41	36.57274441185477	39.0	35.0	41.0	30.0	41.0
42	36.46968675832485	39.0	35.0	40.0	30.0	41.0
43	36.3791509304506	39.0	35.0	40.0	30.0	41.0
44	36.274046414035475	38.0	35.0	40.0	30.0	41.0
45	36.206426257167905	38.0	35.0	40.0	30.0	41.0
46	36.0700455677104	38.0	35.0	40.0	30.0	41.0
47	35.96461387770332	38.0	35.0	40.0	29.0	41.0
48	35.85600306083063	38.0	35.0	40.0	29.0	41.0
49	35.7764647271041	38.0	34.0	40.0	29.0	41.0
50	35.67964635509015	38.0	34.0	40.0	29.0	41.0
51	34.152906335743644	36.0	32.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	7.0
10	6.0
11	9.0
12	11.0
13	22.0
14	31.0
15	57.0
16	103.0
17	195.0
18	435.0
19	730.0
20	1222.0
21	1955.0
22	2806.0
23	4034.0
24	5508.0
25	7505.0
26	10003.0
27	12743.0
28	15817.0
29	19971.0
30	25567.0
31	32971.0
32	43070.0
33	59525.0
34	98337.0
35	120878.0
36	125065.0
37	178569.0
38	295313.0
39	416751.0
40	117.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.65955830213129	17.716112015044576	22.903773785148346	15.720555897675784
2	27.723906047100826	26.06755593891047	27.361311181968677	18.84722683202002
3	32.574592537062216	24.61568932569117	26.11102135618862	16.698696781058
4	29.479827787718417	26.42947482554685	24.931709272464627	19.15898811427011
5	24.737906237726765	30.033116186507876	24.356248779013843	20.87272879675152
6	25.347436047364464	36.426791190918635	23.570829364776248	14.654943396940656
7	82.00139657157226	6.272134070870937	7.12589491103109	4.600574446525707
8	83.3520692039745	4.967022389083758	6.743358680273663	4.937549726668095
9	77.08142228579425	7.530265247201956	9.383325097661993	6.004987369341806
10	39.60882476406661	26.79720712724687	16.844910929693505	16.74905717899302
11	30.592218000360972	26.084387803455193	23.143205368350824	20.18018882783301
12	27.1186349019865	22.972115211070907	29.186993903349943	20.72225598359265
13	24.089507664582175	24.889798605726753	30.519685507764628	20.501008221926444
14	21.100195560578825	28.046618180982424	28.170863028505337	22.68232322993341
15	20.211959817134296	26.869266434896172	32.34982968721799	20.56894406075154
16	22.847261982901802	24.335361043494487	30.68678739191949	22.130589581684227
17	23.091290219875525	24.539776940615965	27.50380744887744	24.865125390631075
18	23.412988386013463	25.423280834590088	28.684539087442552	22.479191691953897
19	24.599398243943064	27.368949738970905	25.92445129135552	22.107200725730515
20	26.087497304535752	25.74335665233818	25.606673800493056	22.562472242633017
21	25.10070389640765	27.370166500263295	25.891193149363534	21.637936453965526
22	23.222700439453618	25.550161998246512	25.83292380302798	25.39421375927189
23	22.58782143622447	27.621562902840935	25.968187100031976	23.82242856090262
24	22.887753094798548	25.22521913532887	28.081836660612154	23.80519110926043
25	23.317337428861713	26.63382312481875	25.940201590307012	24.108637856012525
26	21.81274449297219	26.90590446936702	26.53012802356731	24.751223014093476
27	23.239802695396655	26.39094405128784	26.99898670823484	23.370266545080668
28	22.000058134150635	27.079833736329178	27.498332023061682	23.4217761064585
29	23.319432962198604	27.021699585692783	25.804465108355974	23.85440234375264
30	24.12350938291951	25.100839092106803	27.25450657963669	23.521144945337
31	25.334727651643945	26.453607257845913	24.377744895179397	23.833920195330744
32	25.385899223773894	26.925913432841874	25.06676977591989	22.621417567464345
33	24.081395922632908	26.583395129034155	24.882971222919455	24.45223772541348
34	23.291785441721526	25.995226239862856	26.07140901633637	24.641579302079244
35	22.81778932048614	25.589571544550026	26.810591501463154	24.782047633500685
36	24.05665510968765	27.480080603675837	25.7670158996902	22.696248386946312
37	23.16841936624312	28.351890069673104	26.079993943232676	22.3996966208511
38	22.012698932021575	29.06024793539268	25.54150947350063	23.385543659085116
39	22.78838425592005	26.64659911838885	25.562194415471257	25.00282221021985
40	23.40920290643714	24.617582065479333	28.154369153208496	23.818845874875027
41	21.028271448628676	25.543875398235834	27.813540795640208	25.614312357495283
42	22.549899042611656	26.398041825493447	27.38747154975506	23.664587582139838
43	22.208191912998863	25.462960772291908	28.067911503599248	24.260935811109977
44	23.292799409465186	25.329995802173542	26.689726546419106	24.687478241942166
45	23.327544704147872	24.738176629125075	26.106289506718213	25.82798916000884
46	23.452803519414438	25.26537225797773	26.53168277410759	24.75014144850024
47	22.520493978045568	25.2921410064103	28.380551557893842	23.806813457650286
48	22.112405760147958	25.425917150723603	27.981386256140418	24.480290832988022
49	22.3928692380438	25.294912518242967	28.265364822214277	24.046853421498955
50	21.28764439745643	24.165217256108647	29.01549815897257	25.531640187462358
51	21.287711995306005	24.322247060676506	26.949843071592205	27.44019787242528
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2647.0
1	2546.5
2	2446.0
3	2675.0
4	2904.0
5	2267.5
6	1631.0
7	1815.0
8	1999.0
9	2102.0
10	2205.0
11	2281.0
12	2357.0
13	2468.0
14	2579.0
15	2427.5
16	2276.0
17	2327.5
18	2379.0
19	2620.5
20	2862.0
21	3322.5
22	3783.0
23	4250.5
24	4718.0
25	6305.5
26	9576.0
27	11259.0
28	13661.0
29	16063.0
30	19270.5
31	22478.0
32	25902.0
33	29326.0
34	33772.0
35	38218.0
36	41143.5
37	44069.0
38	47240.5
39	50412.0
40	55755.5
41	61099.0
42	67833.0
43	74567.0
44	81738.0
45	88909.0
46	103358.0
47	117807.0
48	129117.5
49	140428.0
50	137910.0
51	135392.0
52	121241.0
53	107090.0
54	99099.5
55	91109.0
56	85755.5
57	80402.0
58	76890.0
59	73378.0
60	69158.0
61	64938.0
62	59762.0
63	54586.0
64	48179.0
65	41772.0
66	35936.0
67	30100.0
68	25363.0
69	20626.0
70	18090.5
71	15555.0
72	13058.0
73	10561.0
74	8981.5
75	5844.5
76	4287.0
77	3269.5
78	2252.0
79	1824.5
80	1397.0
81	992.0
82	587.0
83	394.0
84	201.0
85	222.5
86	244.0
87	147.5
88	51.0
89	54.0
90	57.0
91	35.5
92	14.0
93	12.0
94	10.0
95	9.5
96	9.0
97	6.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1479337.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.522776975855525
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.20443383166133	17.706532275217484
2	10.588965047691811	5.193416565393513
3	3.99618825126815	2.9399289971814877
4	2.0728643845725476	2.033295640162664
5	1.224248726173608	1.50109892374653
6	0.8298198465444381	1.2209692216168748
7	0.6325877272536021	1.0858965427172387
8	0.517475488447862	1.0151948794943058
9	0.4064698481224583	0.8970992489625221
>10	5.961649396896666	36.34345885501429
>50	1.4500292460901727	23.459850725304367
>100	0.10667850730121414	4.353995935732228
>500	0.004987566566792333	0.819402233782107
>1k	0.0036021314093500185	1.429859955674341
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCTGC	2881	0.19474940463193985	TruSeq Adapter, Index 16 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCTGC	2855	0.1929918605429324	TruSeq Adapter, Index 16 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCTGCT	1980	0.1338437421628743	TruSeq Adapter, Index 13 (95% over 22bp)
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	1770	0.11964819375166037	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	1524	0.1030191227556669	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1481	0.1001124152238469	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.40782796617674E-4	0.0	0.0	0.13911637442989663	0.0
2	5.40782796617674E-4	0.0	0.0	0.6715846355495739	0.0
3	5.40782796617674E-4	0.0	0.0	0.9212234940382077	0.0
4	5.40782796617674E-4	0.0	0.0	1.2677300709709822	0.0
5	5.40782796617674E-4	0.0	0.0	2.2715581371925397	0.0
6	5.40782796617674E-4	0.0	0.0	3.249225835627717	0.0
7	5.40782796617674E-4	0.0	0.0	4.046272079992591	0.0
8	5.40782796617674E-4	0.0	0.0	5.327792112277324	0.0
9	5.40782796617674E-4	0.0	0.0	5.8551905346787105	0.0
10	5.40782796617674E-4	0.0	0.0	6.8602353621926575	0.0
11	5.40782796617674E-4	0.0	0.0	7.979588153341666	0.0
12	5.40782796617674E-4	0.0	0.0	8.933529006575244	0.0
13	5.40782796617674E-4	0.0	0.0	9.29206800073276	0.0
14	5.40782796617674E-4	0.0	0.0	9.446326293467953	0.0
15	5.40782796617674E-4	0.0	0.0	9.699547837984179	0.0
16	5.40782796617674E-4	0.0	0.0	10.258717249686853	0.0
17	5.40782796617674E-4	0.0	0.0	10.930504678785159	0.0
18	5.40782796617674E-4	0.0	0.0	11.725860976910603	0.0
19	5.40782796617674E-4	0.0	0.0	12.138478250729888	0.0
20	5.40782796617674E-4	0.0	0.0	12.564547496615038	0.0
21	5.40782796617674E-4	0.0	0.0	13.059904538316827	0.0
22	5.40782796617674E-4	0.0	0.0	13.589263298355952	0.0
23	5.40782796617674E-4	0.0	0.0	14.138631021869932	0.0
24	5.40782796617674E-4	0.0	0.0	14.585250014026554	0.0
25	6.083806461948832E-4	0.0	0.0	14.954672261966003	0.0
26	6.083806461948832E-4	0.0	0.0	15.316726344301536	0.0
27	6.759784957720925E-4	0.0	0.0	15.663097725535156	0.0
28	6.759784957720925E-4	0.0	0.0	16.041510487468372	0.0
29	8.11174194926511E-4	0.0	0.0	16.419382466604972	0.0
30	8.787720445037203E-4	0.0	0.0	16.846600875932936	0.0
31	8.787720445037203E-4	0.0	0.0	17.232719792717955	0.0
32	8.787720445037203E-4	0.0	0.0	17.624854918115346	0.0
33	8.787720445037203E-4	0.0	0.0	18.014353727379223	0.0
34	8.787720445037203E-4	0.0	0.0	18.38533072585895	0.0
35	8.787720445037203E-4	0.0	0.0	18.821472051331103	0.0
36	8.787720445037203E-4	0.0	0.0	19.21272840468399	0.0
37	8.787720445037203E-4	0.0	0.0	19.61027135804756	0.0
38	9.463698940809295E-4	0.0	0.0	19.99267239310583	0.0
39	9.463698940809295E-4	0.0	0.0	20.387917019583774	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATGCG	30	2.1659253E-6	45.000004	1
CTTACGT	30	2.1659253E-6	45.000004	22
TATACGG	65	0.0	41.538464	2
CGTAAGG	230	0.0	41.086956	2
TTAACGG	105	0.0	40.714287	2
TACGGGT	150	0.0	39.0	4
TACGAAT	290	0.0	38.793102	12
GCTACGA	290	0.0	38.793102	10
GATTACG	65	9.094947E-12	38.076927	1
CTTGACG	95	0.0	37.894737	1
CGAATAT	305	0.0	37.62295	14
ACACGAT	30	1.14008006E-4	37.500004	16
CACAACG	280	0.0	36.964283	12
CGGTCTA	105	0.0	36.42857	31
CGAGGGT	285	0.0	36.31579	4
ATACTAT	480	0.0	36.093754	45
TATTAGG	250	0.0	36.000004	2
AGATACG	75	1.8189894E-12	36.0	1
CCGTAGT	25	0.0021073038	36.0	27
GTTCGGA	25	0.0021073038	36.0	3
>>END_MODULE