##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554028_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1347509
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.15829504663791	33.0	31.0	34.0	30.0	34.0
2	32.27090431306952	34.0	31.0	34.0	30.0	34.0
3	32.28917728935391	34.0	31.0	34.0	30.0	34.0
4	35.8467201332236	37.0	35.0	37.0	35.0	37.0
5	35.72130278907228	37.0	35.0	37.0	35.0	37.0
6	35.71520487061682	37.0	35.0	37.0	33.0	37.0
7	35.91411634356431	37.0	35.0	37.0	35.0	37.0
8	35.76208025326733	37.0	35.0	37.0	35.0	37.0
9	37.451948001831525	39.0	37.0	39.0	35.0	39.0
10	37.085809445428566	39.0	37.0	39.0	33.0	39.0
11	37.05736881905798	39.0	37.0	39.0	33.0	39.0
12	36.96308521872581	39.0	37.0	39.0	33.0	39.0
13	36.96102289483781	39.0	37.0	39.0	33.0	39.0
14	38.10736774299838	40.0	37.0	41.0	33.0	41.0
15	38.22816099929574	40.0	37.0	41.0	33.0	41.0
16	38.26855033992352	40.0	37.0	41.0	34.0	41.0
17	38.21134997985171	40.0	37.0	41.0	34.0	41.0
18	38.188465531584576	40.0	37.0	41.0	34.0	41.0
19	38.175778417806484	40.0	37.0	41.0	34.0	41.0
20	38.12274945844518	40.0	37.0	41.0	34.0	41.0
21	38.01591232414774	40.0	37.0	41.0	33.0	41.0
22	38.01751453979157	40.0	37.0	41.0	33.0	41.0
23	37.95153650179702	40.0	37.0	41.0	33.0	41.0
24	37.92311888083864	40.0	36.0	41.0	33.0	41.0
25	37.824568147596786	40.0	36.0	41.0	33.0	41.0
26	37.779855273693904	40.0	36.0	41.0	33.0	41.0
27	37.73366114808881	40.0	36.0	41.0	33.0	41.0
28	37.63882541786363	40.0	36.0	41.0	33.0	41.0
29	37.61625117160627	40.0	36.0	41.0	33.0	41.0
30	37.497074231044095	40.0	36.0	41.0	33.0	41.0
31	37.433360370876926	40.0	36.0	41.0	32.0	41.0
32	37.338228538733325	40.0	35.0	41.0	32.0	41.0
33	37.261786748734146	39.0	35.0	41.0	32.0	41.0
34	37.146416090727406	39.0	35.0	41.0	31.0	41.0
35	37.088371209394516	39.0	35.0	41.0	31.0	41.0
36	36.99495439362557	39.0	35.0	41.0	31.0	41.0
37	36.93886051967	39.0	35.0	41.0	31.0	41.0
38	36.82053477935954	39.0	35.0	41.0	31.0	41.0
39	36.780287923865444	39.0	35.0	41.0	31.0	41.0
40	36.6408343098265	39.0	35.0	41.0	31.0	41.0
41	36.516978365265096	39.0	35.0	41.0	30.0	41.0
42	36.43636517455542	39.0	35.0	41.0	30.0	41.0
43	36.35187000606304	39.0	35.0	40.0	30.0	41.0
44	36.2543849428835	38.0	35.0	40.0	30.0	41.0
45	36.195184596169675	38.0	35.0	40.0	30.0	41.0
46	36.051711713984844	38.0	35.0	40.0	30.0	41.0
47	35.95368862100364	38.0	35.0	40.0	29.0	41.0
48	35.84153278382556	38.0	35.0	40.0	29.0	41.0
49	35.75889363262138	38.0	34.0	40.0	29.0	41.0
50	35.665526538227205	38.0	34.0	40.0	29.0	41.0
51	34.193381268696534	36.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	8.0
10	6.0
11	13.0
12	16.0
13	14.0
14	44.0
15	54.0
16	91.0
17	170.0
18	342.0
19	664.0
20	1131.0
21	1722.0
22	2588.0
23	3709.0
24	5100.0
25	7073.0
26	9154.0
27	11451.0
28	14222.0
29	17645.0
30	22595.0
31	29070.0
32	37796.0
33	52289.0
34	88113.0
35	106601.0
36	110906.0
37	161868.0
38	267746.0
39	395174.0
40	133.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.89573650342966	17.685670374001212	22.012988410467017	16.40560471210211
2	27.78341369148555	26.200196065480824	27.26237821046093	18.754012032572696
3	32.48067359846947	24.7027663637126	25.813482507352454	17.003077530465475
4	29.020511180259277	26.29763511783595	25.272335843396966	19.40951785850781
5	25.126882269431967	31.231776559562867	23.76844978400886	19.8728913869963
6	25.15894142450997	36.21541674304216	23.506781772886118	15.118860059561753
7	82.77043047578903	6.0210358520796525	6.893683084862514	4.314850587268805
8	84.39053097233487	5.055179594347793	6.169977343379525	4.384312089937803
9	78.0529851748671	7.587407579467002	8.488254994957362	5.871352250708529
10	40.80692596487297	26.636185732340188	15.97258348552774	16.584304817259106
11	31.122983223117618	26.48279158061282	22.425378977060635	19.968846219208924
12	27.101711380035308	23.75665023387599	28.50838102008966	20.63325736599904
13	24.292973182368353	24.28102521022123	29.86466138630614	21.561340221104274
14	21.23080439536953	27.548016376885055	28.82578149756328	22.395397730182136
15	21.325275007439654	26.52924767107307	30.901389155842374	21.244088165644907
16	23.740546445329862	25.101502104995216	29.70043242753852	21.457519022136402
17	22.997916897030002	25.367919620573964	27.4196313345588	24.214532147837232
18	24.386182207317354	25.212818615682714	27.836474561579923	22.56452461542001
19	25.433893205908088	27.514621423678804	25.264914742684464	21.786570627728647
20	26.416966417292947	26.223053055675322	25.59552477942633	21.764455747605396
21	25.89192354188358	27.23284222962518	25.42090627966121	21.454327948830027
22	24.038058372893982	25.06647450963222	25.666618924252084	25.228848193221715
23	23.98514592481386	26.488876883197072	25.977637255112953	23.54833993687612
24	23.52963876308062	25.685616942076084	27.597069852594675	23.187674442248625
25	23.36014082280712	26.925386027106313	25.46736237012146	24.247110779965105
26	22.02894377699889	27.418295536430552	25.986765208989326	24.565995477581225
27	23.90796647740386	26.12902770964795	26.587651733680445	23.375354079267748
28	22.43510061899401	27.617848934589677	27.180968735644807	22.766081710771505
29	23.531494038258742	26.44909978337807	26.395519436233823	23.623886742129365
30	24.966215439006344	25.748918931153707	26.63277202601244	22.652093603827506
31	26.45577877401932	27.186089295136433	23.52963876308062	22.82849316776363
32	25.847619570629952	27.6149547053118	24.759611995170346	21.777813728887896
33	25.690737501567707	27.02579351974644	23.774015609543238	23.50945336914262
34	24.172899772840108	27.257851339026306	25.617639659549585	22.951609228584
35	25.895708303246955	26.214221945827447	25.45430123286746	22.435768518058136
36	25.71433660183346	29.358393895699397	23.915387578116363	21.011881924350785
37	25.640051383701334	29.020214336230776	24.328594465788356	21.011139814279534
38	24.278130980943356	29.78926300306714	24.244884449751357	21.687721566238146
39	25.971255108500202	26.423348563905698	24.69816528127085	22.907231046323254
40	25.269367403111964	25.734596206778583	27.01785294198406	21.978183448125392
41	22.207569671148764	27.27692356785743	26.565091587514445	23.95041517347936
42	22.70099865752288	28.022150501406667	26.038787124983955	23.238063716086497
43	23.63034309974924	26.113072343116077	26.33288534622032	23.92369921091436
44	23.640064741682615	26.207468744179074	26.25555747679607	23.896909037342237
45	25.105657921394215	25.17222519478534	25.42046101361846	24.30165587020198
46	24.683842556895723	26.36056605187795	26.027432840893827	22.928158550332505
47	24.287184723812604	25.991811557473827	27.081154931061686	22.639848787651882
48	22.686304878112132	26.263943320601197	27.228537991211933	23.821213810074738
49	23.171051176652625	26.697632446239687	26.95640622808456	23.174910149023123
50	22.598884311718884	25.528660662006708	27.7583303710773	24.11412465519711
51	22.746490004890504	24.97949920928172	26.90260324791894	25.371407537908837
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2090.0
1	2162.0
2	2234.0
3	2653.0
4	3072.0
5	2337.0
6	1602.0
7	1714.5
8	1827.0
9	2022.0
10	2217.0
11	2215.5
12	2214.0
13	2166.5
14	2119.0
15	2135.0
16	2151.0
17	2180.5
18	2210.0
19	2351.5
20	2493.0
21	3218.0
22	3943.0
23	4651.5
24	5360.0
25	6821.0
26	9783.0
27	11284.0
28	13936.5
29	16589.0
30	19487.0
31	22385.0
32	25351.5
33	28318.0
34	31190.0
35	34062.0
36	36587.0
37	39112.0
38	41272.5
39	43433.0
40	49584.0
41	55735.0
42	61758.0
43	67781.0
44	74305.0
45	80829.0
46	90169.5
47	99510.0
48	107462.5
49	115415.0
50	114702.5
51	113990.0
52	103804.5
53	93619.0
54	86690.0
55	79761.0
56	76463.0
57	73165.0
58	70846.0
59	68527.0
60	66346.0
61	64165.0
62	60866.0
63	57567.0
64	51391.0
65	45215.0
66	37582.0
67	29949.0
68	25884.5
69	21820.0
70	18455.5
71	15091.0
72	13445.5
73	11800.0
74	9494.5
75	5696.0
76	4203.0
77	3247.0
78	2291.0
79	1802.0
80	1313.0
81	1113.5
82	914.0
83	630.5
84	347.0
85	236.5
86	126.0
87	97.0
88	68.0
89	56.0
90	44.0
91	34.0
92	24.0
93	17.0
94	10.0
95	14.0
96	18.0
97	15.0
98	12.0
99	13.0
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1347509.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.731173911326838
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.00638161889371	18.055335204535552
2	9.547597462242827	4.722465865481402
3	3.4270175027178094	2.542624975706254
4	1.751695647668478	1.732859588088137
5	1.1444107901754537	1.4151311138914053
6	0.8438763898267853	1.252203225388132
7	0.6613364844862962	1.1448939328215266
8	0.5699335582905714	1.127610075838836
9	0.49465102307770287	1.1009970429435374
>10	7.3862140616138845	44.80116795346966
>50	1.0795129110434345	16.71590970098497
>100	0.07923786427780724	3.4501680867205673
>500	0.005121839135140546	0.8901856886829217
>1k	0.003012846550082674	1.0484475454471742
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTCTGC	1955	0.14508251892937266	No Hit
GCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTCTGC	1868	0.13862616130949776	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	1760	0.13061137253999788	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1463	0.10857070342387323	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.09447061207012346	0.0
2	0.0	0.0	0.0	0.4635219505027425	0.0
3	0.0	0.0	0.0	0.6463778720587395	0.0
4	0.0	0.0	0.0	0.9104948464166102	0.0
5	0.0	0.0	0.0	1.7035878795614723	0.0
6	0.0	0.0	0.0	2.5097420499603342	0.0
7	0.0	0.0	0.0	3.146249858071449	0.0
8	0.0	0.0	0.0	4.130584656577433	0.0
9	0.0	0.0	0.0	4.551583699997551	0.0
10	0.0	0.0	0.0	5.383711722890163	0.0
11	0.0	0.0	0.0	6.3020729360620225	0.0
12	0.0	0.0	0.0	7.06852421764901	0.0
13	1.4842201424999758E-4	0.0	0.0	7.370711438662005	0.0
14	1.4842201424999758E-4	0.0	0.0	7.498131737895628	0.0
15	1.4842201424999758E-4	0.0	0.0	7.718538429056874	0.0
16	1.4842201424999758E-4	0.0	0.0	8.150149646495867	0.0
17	1.4842201424999758E-4	0.0	0.0	8.67942254931136	0.0
18	1.4842201424999758E-4	0.0	0.0	9.280012229973973	0.0
19	1.4842201424999758E-4	0.0	0.0	9.634815055038594	0.0
20	1.4842201424999758E-4	0.0	0.0	9.974107779614087	0.0
21	1.4842201424999758E-4	0.0	0.0	10.37781565837408	0.0
22	1.4842201424999758E-4	0.0	0.0	10.803489995243075	0.0
23	1.4842201424999758E-4	0.0	0.0	11.240592827209317	0.0
24	1.4842201424999758E-4	0.0	0.0	11.591388257889186	0.0
25	1.4842201424999758E-4	0.0	0.0	11.911311909605056	0.0
26	1.4842201424999758E-4	0.0	0.0	12.200734837392552	0.0
27	1.4842201424999758E-4	0.0	0.0	12.507523140847297	0.0
28	1.4842201424999758E-4	0.0	0.0	12.830192599826791	0.0
29	1.4842201424999758E-4	0.0	0.0	13.136090371196037	0.0
30	1.4842201424999758E-4	0.0	0.0	13.52080023213203	0.0
31	1.4842201424999758E-4	0.0	0.0	13.85126184685965	0.0
32	2.9684402849999516E-4	0.0	0.0	14.194933020855519	0.0
33	2.9684402849999516E-4	0.0	0.0	14.52287146134089	0.0
34	3.7105503562499397E-4	0.0	0.0	14.850735690819134	0.0
35	3.7105503562499397E-4	0.0	0.0	15.203683240705628	0.0
36	3.7105503562499397E-4	0.0	0.0	15.527465864791997	0.0
37	4.4526604274999277E-4	0.0	0.0	15.864606470160867	0.0
38	4.4526604274999277E-4	0.0	0.0	16.200188644380113	0.0
39	5.194770498749916E-4	0.0	0.0	16.53228290126448	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGCAA	20	7.033719E-4	45.000004	32
CGGTTTA	20	7.033719E-4	45.000004	21
GTTACGT	20	7.033719E-4	45.000004	12
AATGCGC	20	7.033719E-4	45.000004	33
CCCGTAG	20	7.033719E-4	45.000004	13
CGATACC	20	7.033719E-4	45.000004	10
GTATACG	45	3.8562575E-10	45.0	1
ATACGGT	25	3.8911654E-5	45.0	28
CAATACG	35	1.2121382E-7	45.0	1
TTACGAG	80	0.0	42.187504	1
CATCGCG	45	1.9286745E-8	40.0	1
TTCGCAT	45	1.9286745E-8	40.0	25
ATGTACG	40	3.4588084E-7	39.375004	1
ATTAACG	35	6.249091E-6	38.571426	1
TTACGCG	35	6.249091E-6	38.571426	1
ATAACGG	65	9.094947E-12	38.076927	2
ACGATAG	60	1.5643309E-10	37.500004	1
CACGACC	350	0.0	37.285713	27
GATCGAC	85	0.0	37.058823	23
AGGGATC	645	0.0	36.627907	6
>>END_MODULE