FastQCFastQC Report
Sat 18 Jun 2016
SRR3554026_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554026_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1119323
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCG146161.3057893029983303No Hit
GAATCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTC139991.2506666976377685No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGC116751.0430411954368846No Hit
GCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTGC49790.4448224507135117TruSeq Adapter, Index 20 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCT31620.28249218500825946No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCCTGTTTT28480.2544395138847321No Hit
CCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTGC24730.22093712002701632TruSeq Adapter, Index 20 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTC23390.20896559795519254No Hit
GAACTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCT22460.20065700427847905No Hit
CTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTGCT17770.15875667702709584TruSeq Adapter, Index 22 (95% over 23bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTA17520.15652318410324814No Hit
GAATGCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTT14930.13338419741218574No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTACGT207.033207E-445.044
TCAATCG207.033207E-445.019
CCCGTAA207.033207E-445.041
ACCCGAA207.033207E-445.016
CCAACGT253.8907398E-545.032
ATACGGT351.2118835E-745.028
TCGACGT207.033207E-445.026
TATAACG253.8907398E-545.01
CGTATGG603.6379788E-1241.2500042
CCGATGA12150.040.18518418
CGATGAA12500.040.1419
TAGGACG750.039.01
TACGGCT14700.038.8775527
CATACGA1400.038.57142618
GCGATGT2750.038.4545449
TGATCCG2950.038.1355934
GATGAAT13600.038.0514720
CGCATCG301.13990274E-437.50000421
ATAGCGC301.13990274E-437.50000412
TCGATTG301.13990274E-437.5000041
Produced by FastQC (version 0.11.5)