##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554026_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1119323
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.833573508272412	31.0	31.0	34.0	30.0	34.0
2	31.933320408854282	33.0	31.0	34.0	30.0	34.0
3	31.981738068457453	33.0	31.0	34.0	30.0	34.0
4	35.60116338179417	37.0	35.0	37.0	33.0	37.0
5	35.452899654523314	37.0	35.0	37.0	33.0	37.0
6	35.41425307976339	37.0	35.0	37.0	33.0	37.0
7	35.66134976231168	37.0	35.0	37.0	35.0	37.0
8	35.343130624493554	37.0	35.0	37.0	33.0	37.0
9	37.10590062028565	39.0	37.0	39.0	34.0	39.0
10	36.68515254309971	39.0	35.0	39.0	32.0	39.0
11	36.69766099687043	39.0	35.0	39.0	32.0	39.0
12	36.62147119285497	39.0	35.0	39.0	32.0	39.0
13	36.537304245512686	39.0	35.0	39.0	32.0	39.0
14	37.5494669545788	39.0	36.0	41.0	32.0	41.0
15	37.64152438572244	39.0	36.0	41.0	32.0	41.0
16	37.791473060055054	40.0	36.0	41.0	33.0	41.0
17	37.67346958831365	39.0	36.0	41.0	33.0	41.0
18	37.67419055982947	39.0	36.0	41.0	33.0	41.0
19	37.6610853167495	39.0	36.0	41.0	33.0	41.0
20	37.48013308044237	39.0	36.0	41.0	32.0	41.0
21	37.46816513195923	39.0	36.0	41.0	32.0	41.0
22	37.49419961887677	39.0	36.0	41.0	32.0	41.0
23	37.35068429756201	39.0	35.0	41.0	32.0	41.0
24	37.335123999060144	39.0	35.0	41.0	32.0	41.0
25	37.29738779601598	39.0	35.0	41.0	32.0	41.0
26	37.22147226493157	39.0	35.0	41.0	32.0	41.0
27	37.140247274468585	39.0	35.0	41.0	31.0	41.0
28	37.025867421646836	39.0	35.0	41.0	31.0	41.0
29	37.0191374607687	39.0	35.0	41.0	31.0	41.0
30	36.905448203959	39.0	35.0	41.0	31.0	41.0
31	36.86316103573321	39.0	35.0	41.0	31.0	41.0
32	36.72394474159827	39.0	35.0	41.0	30.0	41.0
33	36.647043793435856	39.0	35.0	41.0	30.0	41.0
34	36.497459625148416	39.0	35.0	41.0	30.0	41.0
35	36.41324890134483	39.0	35.0	40.0	30.0	41.0
36	36.33367044186531	39.0	35.0	40.0	30.0	41.0
37	36.27227082799156	39.0	35.0	40.0	30.0	41.0
38	36.25549997632498	39.0	35.0	40.0	30.0	41.0
39	36.06150235454824	38.0	35.0	40.0	29.0	41.0
40	35.99683916081417	38.0	35.0	40.0	29.0	41.0
41	35.84875857996307	38.0	35.0	40.0	28.0	41.0
42	35.82804159299862	38.0	35.0	40.0	29.0	41.0
43	35.81500424810354	38.0	35.0	40.0	29.0	41.0
44	35.77068460131704	38.0	35.0	40.0	28.0	41.0
45	35.708388016685085	38.0	34.0	40.0	28.0	41.0
46	35.5730445992801	38.0	34.0	40.0	28.0	41.0
47	35.3793480523495	38.0	34.0	40.0	27.0	41.0
48	35.36274962633664	38.0	34.0	40.0	27.0	41.0
49	35.32554410121118	38.0	34.0	40.0	27.0	41.0
50	35.18683257647703	38.0	34.0	40.0	27.0	41.0
51	33.70884722283023	36.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	1.0
11	6.0
12	12.0
13	14.0
14	21.0
15	47.0
16	72.0
17	193.0
18	346.0
19	759.0
20	1542.0
21	2651.0
22	4309.0
23	6062.0
24	7267.0
25	8466.0
26	9974.0
27	11966.0
28	14386.0
29	18368.0
30	23064.0
31	29762.0
32	37810.0
33	50767.0
34	78313.0
35	93503.0
36	97901.0
37	136849.0
38	217392.0
39	267425.0
40	72.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.36899000556586	16.648188235210032	20.784349111025147	15.198472648198955
2	26.397116828654465	30.249356083990055	25.65398906303185	17.69953802432363
3	29.951318788231816	30.42383655120104	24.342750037299332	15.282094623267813
4	26.736250394211503	24.743706686988475	30.61636364123671	17.903679277563313
5	27.96681565553464	28.89755682676046	23.67145140410766	19.464176113597237
6	22.87463046859575	38.94353997907663	24.17907967583977	14.00274987648784
7	76.89121013326805	5.926260784420583	12.507649713264179	4.674879369047183
8	77.27769374881066	9.136683513159294	8.056119636601768	5.529503101428275
9	71.74702923106199	6.934548829962398	9.584454174532283	11.733967764443328
10	39.912607888875684	24.26984882826494	18.632870047341115	17.184673235518254
11	31.596330996504136	24.468540358770436	24.07875117370053	19.856377471024896
12	25.883413456169492	20.916214533249118	29.930681313615466	23.269690696965935
13	24.8756614489294	23.819576654817244	32.985384915703506	18.31937698054985
14	21.49334910477137	28.750950351239098	28.223399322626264	21.53230122136327
15	19.449792419167657	24.936233777024146	35.35217269724646	20.261801106561734
16	22.37959909695414	24.497039728478732	28.653391380325427	24.4699697942417
17	22.340378961211375	25.99151451368372	28.090729842949713	23.577376682155197
18	23.21537214905796	24.12601188396915	29.1818358061078	23.476780160865093
19	22.598124044623404	27.244861402830107	25.66676464255626	24.490249909990236
20	24.14298643019039	26.39023767044901	28.417534527567113	21.049241371793485
21	22.9158160781115	30.47842311825988	27.277827758386096	19.327933045242528
22	21.254276022202706	23.91651024771223	28.747287422843986	26.081926307241073
23	21.631110948314294	28.413871599171998	27.681285920149946	22.273731532363758
24	22.130341286652737	25.91477169682031	27.76106628738979	24.193820729137165
25	20.884230914579614	30.369964701877834	25.493534931382634	23.252269452159922
26	21.22756344683349	25.506042491756176	28.38153062163468	24.88486343977565
27	23.742565818802973	26.178681220702156	25.19129866892756	24.887454291567312
28	19.860129739136962	26.43035120336132	29.07668295925305	24.632836098248674
29	24.432804471988874	23.587472070170985	27.373421255526782	24.606302202313362
30	23.276212496303568	25.92870869266512	26.612157527362523	24.18292128366879
31	23.931429980443536	24.066243613326986	24.49444887668707	27.507877529542412
32	25.53874082816131	25.457084326865438	26.54220452898761	22.461970315985642
33	24.684742473798895	23.0472348017507	25.434481378476097	26.83354134597431
34	24.142093033020853	22.83237278247655	28.827067790083827	24.19846639441877
35	22.46482918692817	22.973082836678955	29.162985125830527	25.39910285056235
36	24.62702901664667	23.91847572148522	28.316223288541376	23.138271973326734
37	20.60888590692767	24.06686899134566	31.91804331725516	23.406201784471506
38	22.651281176210976	24.812944967627754	27.8825683024471	24.653205553714162
39	23.587650749604894	22.908043522736513	31.219228051241686	22.285077676416908
40	24.673485669462703	22.087815581382674	30.950315503210422	22.2883832459442
41	20.158524393763017	23.835925823019807	30.67845474451968	25.3270950386975
42	20.862074664775047	23.17132766859968	32.18400765462695	23.782590011998327
43	22.72900672996088	21.902882367288086	29.83142488807967	25.536686014671368
44	23.125853752670142	22.30902072056055	29.32924633908175	25.23587918768756
45	22.1581259386254	21.683464022449282	28.3155979105227	27.842812128402617
46	25.724746118859347	24.08080598719047	27.11996447852854	23.074483415421643
47	20.703139308314043	22.893213129722163	33.13074063518752	23.272906926776272
48	22.053687809506282	23.78687831841211	28.575576486858573	25.58385738522303
49	22.07307452808528	21.699098472916216	32.28263870214406	23.94518829685444
50	21.878760643710528	21.43715442280736	31.054396273461727	25.62968866002039
51	21.845615608720628	21.63352312067205	27.933134582243017	28.587726688364306
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1696.0
1	1682.5
2	1669.0
3	1849.5
4	2030.0
5	1586.0
6	1142.0
7	1260.0
8	1378.0
9	1512.0
10	1646.0
11	1701.5
12	1757.0
13	1746.0
14	1735.0
15	1731.0
16	1727.0
17	1648.5
18	1570.0
19	1624.0
20	1678.0
21	2042.0
22	2406.0
23	2717.5
24	3029.0
25	4203.5
26	6240.5
27	7103.0
28	8856.0
29	10609.0
30	12439.0
31	14269.0
32	15964.5
33	17660.0
34	20855.5
35	24051.0
36	26816.5
37	29582.0
38	32379.0
39	35176.0
40	38798.5
41	42421.0
42	49446.0
43	56471.0
44	64424.5
45	72378.0
46	108083.5
47	143789.0
48	130764.5
49	117740.0
50	115352.5
51	112965.0
52	99906.0
53	86847.0
54	78430.5
55	70014.0
56	63629.0
57	57244.0
58	52336.0
59	47428.0
60	43788.5
61	40149.0
62	35844.5
63	31540.0
64	27466.5
65	23393.0
66	19838.0
67	16283.0
68	13902.0
69	11521.0
70	9773.5
71	8026.0
72	6648.0
73	5270.0
74	4508.0
75	3004.0
76	2262.0
77	1770.0
78	1278.0
79	1016.0
80	754.0
81	515.0
82	276.0
83	208.0
84	140.0
85	93.0
86	46.0
87	32.0
88	18.0
89	15.5
90	13.0
91	10.0
92	7.0
93	6.0
94	5.0
95	5.0
96	5.0
97	3.5
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1119323.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.265517489283262
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.35084635988757	21.466504769990188
2	10.294119115914866	6.025254460471433
3	3.9187404376692796	3.440519004437155
4	2.023762695012378	2.3690585018017507
5	1.225619135640778	1.7934189124647708
6	0.8686170547182113	1.5252316563807315
7	0.7017264708216782	1.4375471813167389
8	0.5534608554005613	1.295785467468704
9	0.4690168260976543	1.235341811423612
>10	6.0193902131741535	40.71677940382463
>50	0.510572655563117	9.20478742248654
>100	0.05615051176211903	2.940202924007942
>500	0.003988834168826374	0.7622814837265419
>1k	0.0030683339760202875	2.1693907839968816
>5k	0.0	0.0
>10k+	9.205001928060861E-4	3.6178962162024235
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCG	14616	1.3057893029983303	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTC	13999	1.2506666976377685	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGC	11675	1.0430411954368846	No Hit
GCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTGC	4979	0.4448224507135117	TruSeq Adapter, Index 20 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCT	3162	0.28249218500825946	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCCTGTTTT	2848	0.2544395138847321	No Hit
CCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTGC	2473	0.22093712002701632	TruSeq Adapter, Index 20 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTC	2339	0.20896559795519254	No Hit
GAACTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCT	2246	0.20065700427847905	No Hit
CTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTGCT	1777	0.15875667702709584	TruSeq Adapter, Index 22 (95% over 23bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTA	1752	0.15652318410324814	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTT	1493	0.13338419741218574	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.933971695390875E-5	0.0	0.0	0.16492111749691554	0.0
2	8.933971695390875E-5	0.0	0.0	1.019187491010191	0.0
3	8.933971695390875E-5	0.0	0.0	1.3143659158259056	0.0
4	8.933971695390875E-5	0.0	0.0	1.890607090178617	0.0
5	8.933971695390875E-5	0.0	0.0	4.277317628602289	0.0
6	8.933971695390875E-5	0.0	0.0	5.403266081372401	0.0
7	8.933971695390875E-5	0.0	0.0	6.545117003760309	0.0
8	8.933971695390875E-5	0.0	0.0	8.095071753193672	0.0
9	8.933971695390875E-5	0.0	0.0	8.619317212279208	0.0
10	8.933971695390875E-5	0.0	0.0	11.101621247843562	0.0
11	8.933971695390875E-5	0.0	0.0	12.31753479558626	0.0
12	1.786794339078175E-4	0.0	0.0	14.565143394712697	0.0
13	1.786794339078175E-4	0.0	0.0	15.070002135219235	0.0
14	1.786794339078175E-4	0.0	0.0	15.298622470904288	0.0
15	1.786794339078175E-4	0.0	0.0	15.77524986085339	0.0
16	1.786794339078175E-4	0.0	0.0	16.457805298381253	0.0
17	2.6801915086172625E-4	0.0	0.0	17.273566253887395	0.0
18	2.6801915086172625E-4	0.0	0.0	18.187332878891972	0.0
19	2.6801915086172625E-4	0.0	0.0	18.976023900161078	0.0
20	2.6801915086172625E-4	0.0	0.0	19.50723785716902	0.0
21	3.57358867815635E-4	0.0	0.0	20.0953612138766	0.0
22	3.57358867815635E-4	0.0	0.0	20.775593818763664	0.0
23	3.57358867815635E-4	0.0	0.0	21.40749363677866	0.0
24	3.57358867815635E-4	0.0	0.0	21.88885603172632	0.0
25	3.57358867815635E-4	0.0	0.0	22.33984292290965	0.0
26	3.57358867815635E-4	0.0	0.0	22.787524244565688	0.0
27	5.360383017234525E-4	0.0	0.0	23.262632859326576	0.0
28	6.253780186773612E-4	0.0	0.0	23.692267558157923	0.0
29	6.253780186773612E-4	0.0	0.0	24.145845301132915	0.0
30	6.253780186773612E-4	0.0	0.0	24.667946607011558	0.0
31	6.253780186773612E-4	0.0	0.0	25.120630952817017	0.0
32	7.1471773563127E-4	0.0	0.0	25.559556982211568	0.0
33	7.1471773563127E-4	0.0	0.0	26.0002698059452	0.0
34	7.1471773563127E-4	0.0	0.0	26.43749838071763	0.0
35	7.1471773563127E-4	0.0	0.0	26.915287186987133	0.0
36	7.1471773563127E-4	0.0	0.0	27.332414325444933	0.0
37	7.1471773563127E-4	0.0	0.0	27.76660534984093	0.0
38	8.040574525851787E-4	0.0	0.0	28.216341484986906	0.0
39	8.040574525851787E-4	0.0	0.0	28.746126006523586	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTACGT	20	7.033207E-4	45.0	44
TCAATCG	20	7.033207E-4	45.0	19
CCCGTAA	20	7.033207E-4	45.0	41
ACCCGAA	20	7.033207E-4	45.0	16
CCAACGT	25	3.8907398E-5	45.0	32
ATACGGT	35	1.2118835E-7	45.0	28
TCGACGT	20	7.033207E-4	45.0	26
TATAACG	25	3.8907398E-5	45.0	1
CGTATGG	60	3.6379788E-12	41.250004	2
CCGATGA	1215	0.0	40.185184	18
CGATGAA	1250	0.0	40.14	19
TAGGACG	75	0.0	39.0	1
TACGGCT	1470	0.0	38.877552	7
CATACGA	140	0.0	38.571426	18
GCGATGT	275	0.0	38.454544	9
TGATCCG	295	0.0	38.135593	4
GATGAAT	1360	0.0	38.05147	20
CGCATCG	30	1.13990274E-4	37.500004	21
ATAGCGC	30	1.13990274E-4	37.500004	12
TCGATTG	30	1.13990274E-4	37.500004	1
>>END_MODULE