FastQCFastQC Report
Sat 18 Jun 2016
SRR3554025_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554025_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences857732
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGC14460.16858412651037852RNA PCR Primer, Index 23 (95% over 23bp)
GAATCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTC10500.12241585949923751No Hit
CCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGC10380.12101682110496052RNA PCR Primer, Index 1 (95% over 22bp)
GAATGATACCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCG9600.11192307154216001No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT8830.10294590851221595No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCACG253.8899747E-545.01
TATTACG253.8899747E-545.01
TCGTAAG253.8899747E-545.01
ACGTCGT207.032284E-445.037
GCGATAT603.6379788E-1241.2500049
CGTAAGG1450.040.3448262
TGTTCGG451.9274012E-840.02
TAGGGCC2200.039.8863645
TAAGCGA403.456862E-739.37513
TATGCGG1050.038.571432
TCGATTA356.246435E-638.57142641
GTACGAG950.037.8947371
CCATGCG301.1396803E-437.5000041
GTACGAC301.1396803E-437.50000420
TTAACGG900.037.52
AGGGTAC1950.036.923086
ATAGGGC3050.036.8852464
GCGTAAG1100.036.818181
ACGGGTA1350.036.6666685
TAACGGG2650.036.5094343
Produced by FastQC (version 0.11.5)