##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3554025_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 857732 Sequences flagged as poor quality 0 Sequence length 51 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.08265402246856 33.0 31.0 34.0 30.0 34.0 2 32.196601036221104 34.0 31.0 34.0 30.0 34.0 3 32.219418186566436 34.0 31.0 34.0 30.0 34.0 4 35.78989124808215 37.0 35.0 37.0 35.0 37.0 5 35.65389072577448 37.0 35.0 37.0 33.0 37.0 6 35.642564344107484 37.0 35.0 37.0 33.0 37.0 7 35.838971846684046 37.0 35.0 37.0 35.0 37.0 8 35.68569553193772 37.0 35.0 37.0 35.0 37.0 9 37.376548852088995 39.0 37.0 39.0 35.0 39.0 10 36.98092527735936 39.0 37.0 39.0 33.0 39.0 11 36.95944071108458 39.0 37.0 39.0 33.0 39.0 12 36.859409465893776 39.0 37.0 39.0 33.0 39.0 13 36.81279000900048 39.0 37.0 39.0 33.0 39.0 14 37.95738878810631 40.0 37.0 41.0 33.0 41.0 15 38.08520143821147 40.0 37.0 41.0 33.0 41.0 16 38.139941147118215 40.0 37.0 41.0 33.0 41.0 17 38.0977986130866 40.0 37.0 41.0 33.0 41.0 18 38.063200393596134 40.0 37.0 41.0 33.0 41.0 19 38.02959782309626 40.0 37.0 41.0 33.0 41.0 20 37.976950842454286 40.0 37.0 41.0 33.0 41.0 21 37.863907374331376 40.0 36.0 41.0 33.0 41.0 22 37.84502385360462 40.0 36.0 41.0 33.0 41.0 23 37.77442954209474 40.0 36.0 41.0 33.0 41.0 24 37.76024679037275 40.0 36.0 41.0 33.0 41.0 25 37.64408579836126 40.0 36.0 41.0 33.0 41.0 26 37.61229498258197 40.0 36.0 41.0 33.0 41.0 27 37.562006547499685 40.0 36.0 41.0 33.0 41.0 28 37.44834400488731 40.0 36.0 41.0 32.0 41.0 29 37.41465282862246 39.0 36.0 41.0 32.0 41.0 30 37.31637271315516 39.0 36.0 41.0 32.0 41.0 31 37.233977512789544 39.0 35.0 41.0 32.0 41.0 32 37.14617852662603 39.0 35.0 41.0 32.0 41.0 33 37.086193589606076 39.0 35.0 41.0 31.0 41.0 34 36.97185601096846 39.0 35.0 41.0 31.0 41.0 35 36.911419884066355 39.0 35.0 41.0 31.0 41.0 36 36.81733105445524 39.0 35.0 41.0 31.0 41.0 37 36.737262921285435 39.0 35.0 41.0 31.0 41.0 38 36.6260102223072 39.0 35.0 41.0 31.0 41.0 39 36.56066580237184 39.0 35.0 41.0 30.0 41.0 40 36.43860786352847 39.0 35.0 41.0 30.0 41.0 41 36.32986643846796 39.0 35.0 40.0 30.0 41.0 42 36.24992538461897 38.0 35.0 40.0 30.0 41.0 43 36.13901894764332 38.0 35.0 40.0 30.0 41.0 44 36.041683183092154 38.0 35.0 40.0 29.0 41.0 45 35.95090774274482 38.0 35.0 40.0 29.0 41.0 46 35.78654871218516 38.0 34.0 40.0 29.0 41.0 47 35.67770935443705 38.0 34.0 40.0 28.0 41.0 48 35.57276981621299 38.0 34.0 40.0 28.0 41.0 49 35.460983150914274 37.0 34.0 40.0 28.0 41.0 50 35.321848782603425 37.0 34.0 40.0 28.0 41.0 51 33.79888939668801 36.0 32.0 39.0 24.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 4.0 10 9.0 11 3.0 12 8.0 13 9.0 14 24.0 15 29.0 16 70.0 17 148.0 18 277.0 19 498.0 20 845.0 21 1350.0 22 1969.0 23 2748.0 24 3740.0 25 4962.0 26 6244.0 27 7786.0 28 9914.0 29 12428.0 30 15468.0 31 19825.0 32 25465.0 33 35344.0 34 57238.0 35 71650.0 36 73138.0 37 105463.0 38 168952.0 39 232037.0 40 86.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.18851109670619 17.360084501919015 23.052188795567847 15.399215605806942 2 27.82314289311813 25.76177640568383 27.350267915852505 19.064812785345538 3 32.394850606016796 25.114371388732142 26.077492736659003 16.413285268592055 4 28.58142170281626 26.90514053340671 24.995336538685745 19.518101225091286 5 24.816959143415428 30.985436010315574 24.034080575284587 20.16352427098441 6 24.751903858081544 36.62927347936185 23.515387090606392 15.103435571950213 7 82.5382520414302 6.276669169390906 6.832670344583157 4.352408444595747 8 84.4824490633438 5.169563453386372 6.000825432652624 4.347162050617209 9 78.6073039131104 7.0821655248958875 8.270532054301343 6.0399985076923794 10 41.093721582032614 26.811055201391575 15.612102614802758 16.48312060177305 11 31.171158357155846 26.63559246944267 22.120429224979365 20.07281994842212 12 27.099373697145495 23.591634683094487 28.4703147370041 20.83867688275592 13 24.18692551985935 25.650552853338805 29.98174254895469 20.18077907784716 14 21.27366123684321 27.942993848894528 28.295085178120903 22.48825973614136 15 20.865724958378607 26.203056432545363 31.45178214174124 21.479436467334786 16 22.551449636949535 24.787929096734178 30.474553823338756 22.186067442977528 17 23.61425247046863 24.546711560254252 26.965882117024897 24.87315385225222 18 24.96257572295309 24.566181511241272 27.630425354306475 22.840817411499163 19 25.178027635671747 26.96191817490778 25.031245190805517 22.828808998614953 20 26.575900164620187 25.867054044853173 25.271530035022593 22.28551575550405 21 26.183703068091198 26.542673002756107 25.7503509254639 21.523273003688796 22 24.49506372619886 24.80576683626121 25.318048061632304 25.38112137590763 23 23.33386185894895 27.306314792965637 25.303124985426685 24.056698362658732 24 23.20340152868262 25.906693466024354 27.892045534036274 22.997859471256756 25 23.833201979172983 26.7641874151833 25.836974719376215 23.565635886267504 26 22.902608273913064 26.82259726814436 25.640060065381725 24.634734392560848 27 23.862348612387084 26.066999948701923 25.985272789169578 24.08537864974141 28 22.0165506242043 27.132717445542433 27.296871283804265 23.553860646449007 29 24.153814944528126 26.209701864918177 25.826948277550564 23.80953491300313 30 24.805183903596927 25.593775211837734 25.969183847635392 23.63185703692995 31 25.704882177649896 27.675544342521906 22.82216356624214 23.797409913586062 32 25.477188678981317 27.471867669621748 24.142739223906766 22.908204427490173 33 24.96618990547164 26.34167782011164 23.310661138910522 25.381471135506196 34 23.185330616089875 27.72870780150443 25.506801658326843 23.579159924078848 35 24.749222367825848 26.221243931670962 24.97878125102013 24.050752449483056 36 25.011891826351356 28.464951756492702 24.204646672853524 22.318509744302418 37 23.738766887559283 29.224862777650827 24.828151450569642 22.208218884220248 38 24.07173802539721 29.066538266031817 25.07787980394809 21.78384390462289 39 24.90719711984629 26.588258337102964 25.273978352212577 23.230566190838164 40 24.919788465394785 25.275027631008285 27.447967430386182 22.357216473210748 41 22.177906385677577 26.119463888487317 26.760340059599034 24.94228966623607 42 22.956821011691297 26.733291983976347 26.13427037816008 24.17561662617228 43 23.00881860534526 25.21137138406868 26.799396548105936 24.980413462480122 44 23.7435469354064 26.03307326764071 25.74638698334678 24.476992813606117 45 24.678221169316288 24.83607933480388 25.71152761002271 24.774171885857122 46 24.552307713831357 26.095097303120323 25.514962715626794 23.837632267421526 47 23.529260888016303 26.279420611566316 27.1576669635737 23.033651536843678 48 22.97034504950264 26.176474703054105 26.721516744157846 24.13166350328541 49 23.630341412002817 25.87265019843028 27.47478233294316 23.022226056623747 50 22.63236069075189 25.830912219667685 27.882718611407757 23.65400847817267 51 22.531863099429657 25.426706710254486 26.38376555847281 25.657664631843048 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1483.0 1 1495.5 2 1508.0 3 1610.5 4 1713.0 5 1380.5 6 1048.0 7 1093.5 8 1139.0 9 1231.5 10 1324.0 11 1357.5 12 1391.0 13 1389.5 14 1388.0 15 1396.5 16 1405.0 17 1309.5 18 1214.0 19 1450.0 20 1686.0 21 1864.5 22 2043.0 23 2335.5 24 2628.0 25 3886.5 26 5460.0 27 5775.0 28 7396.0 29 9017.0 30 11053.5 31 13090.0 32 14674.0 33 16258.0 34 19026.5 35 21795.0 36 22165.5 37 22536.0 38 24967.5 39 27399.0 40 30739.0 41 34079.0 42 39419.0 43 44759.0 44 48220.0 45 51681.0 46 58422.0 47 65163.0 48 70546.5 49 75930.0 50 74811.0 51 73692.0 52 68667.0 53 63642.0 54 58403.5 55 53165.0 56 51343.5 57 49522.0 58 46413.0 59 43304.0 60 40899.5 61 38495.0 62 35877.5 63 33260.0 64 30034.0 65 26808.0 66 24041.0 67 21274.0 68 17764.0 69 14254.0 70 11813.0 71 9372.0 72 8242.0 73 7112.0 74 6139.0 75 3904.5 76 2643.0 77 2056.5 78 1470.0 79 1219.5 80 969.0 81 754.0 82 539.0 83 391.5 84 244.0 85 163.0 86 82.0 87 65.5 88 49.0 89 39.0 90 29.0 91 25.5 92 22.0 93 17.5 94 13.0 95 9.5 96 6.0 97 3.5 98 1.0 99 1.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 857732.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 26.572079190491976 #Duplication Level Percentage of deduplicated Percentage of total 1 73.5069424231867 19.53232295119851 2 9.042885918425299 4.805765614699635 3 3.2681714494552865 2.6052633168909245 4 1.7315431868854452 1.8404281073470758 5 1.1482461443166248 1.5255643738479214 6 0.8419824514910799 1.3423934626815326 7 0.6976516574210784 1.297663856185961 8 0.570231206412594 1.2121783018948178 9 0.5356594466728151 1.2810226712512638 >10 8.000321389684725 50.774938078016895 >50 0.5970149350540686 9.61882063526985 >100 0.050996857446941234 2.452600669206955 >500 0.007034049303026377 1.298202011759795 >1k 0.0013188842443174458 0.41283594974886306 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGC 1446 0.16858412651037852 RNA PCR Primer, Index 23 (95% over 23bp) GAATCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTC 1050 0.12241585949923751 No Hit CCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGC 1038 0.12101682110496052 RNA PCR Primer, Index 1 (95% over 22bp) GAATGATACCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCG 960 0.11192307154216001 No Hit GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT 883 0.10294590851221595 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 3.4975959856925004E-4 0.0 0.0 0.09303605321942052 0.0 2 3.4975959856925004E-4 0.0 0.0 0.500272812486884 0.0 3 3.4975959856925004E-4 0.0 0.0 0.6777175154943502 0.0 4 3.4975959856925004E-4 0.0 0.0 0.9303605321942052 0.0 5 3.4975959856925004E-4 0.0 0.0 1.7051946295579505 0.0 6 3.4975959856925004E-4 0.0 0.0 2.417071999179231 0.0 7 3.4975959856925004E-4 0.0 0.0 2.9862474525842573 0.0 8 3.4975959856925004E-4 0.0 0.0 3.9469204833211307 0.0 9 3.4975959856925004E-4 0.0 0.0 4.333404839740152 0.0 10 3.4975959856925004E-4 0.0 0.0 5.170729318714937 0.0 11 3.4975959856925004E-4 0.0 0.0 6.032653556122425 0.0 12 3.4975959856925004E-4 0.0 0.0 6.824975633414633 0.0 13 3.4975959856925004E-4 0.0 0.0 7.119006869278516 0.0 14 3.4975959856925004E-4 0.0 0.0 7.244687151697733 0.0 15 4.6634613142566676E-4 0.0 0.0 7.457107814562124 0.0 16 4.6634613142566676E-4 0.0 0.0 7.908997215913596 0.0 17 4.6634613142566676E-4 0.0 0.0 8.448792863038804 0.0 18 4.6634613142566676E-4 0.0 0.0 9.057840910680726 0.0 19 4.6634613142566676E-4 0.0 0.0 9.403286807534288 0.0 20 4.6634613142566676E-4 0.0 0.0 9.752696646504969 0.0 21 4.6634613142566676E-4 0.0 0.0 10.16949350146666 0.0 22 4.6634613142566676E-4 0.0 0.0 10.604361269021092 0.0 23 4.6634613142566676E-4 0.0 0.0 11.055667737708282 0.0 24 4.6634613142566676E-4 0.0 0.0 11.429327575513097 0.0 25 4.6634613142566676E-4 0.0 0.0 11.721959772982704 0.0 26 4.6634613142566676E-4 0.0 0.0 12.004565528626657 0.0 27 4.6634613142566676E-4 0.0 0.0 12.315035465623295 0.0 28 4.6634613142566676E-4 0.0 0.0 12.624456123824224 0.0 29 4.6634613142566676E-4 0.0 0.0 12.933293849360872 0.0 30 5.829326642820834E-4 0.0 0.0 13.306487341034263 0.0 31 5.829326642820834E-4 0.0 0.0 13.638176027010768 0.0 32 5.829326642820834E-4 0.0 0.0 13.958322646234489 0.0 33 5.829326642820834E-4 0.0 0.0 14.284998111298167 0.0 34 5.829326642820834E-4 0.0 0.0 14.607476461179017 0.0 35 5.829326642820834E-4 0.0 0.0 14.981136298983833 0.0 36 5.829326642820834E-4 0.0 0.0 15.306645898718948 0.0 37 5.829326642820834E-4 0.0 0.0 15.628774489001227 0.0 38 5.829326642820834E-4 0.0 0.0 15.953701156072059 0.0 39 5.829326642820834E-4 0.0 0.0 16.279910275004315 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATTCACG 25 3.8899747E-5 45.0 1 TATTACG 25 3.8899747E-5 45.0 1 TCGTAAG 25 3.8899747E-5 45.0 1 ACGTCGT 20 7.032284E-4 45.0 37 GCGATAT 60 3.6379788E-12 41.250004 9 CGTAAGG 145 0.0 40.344826 2 TGTTCGG 45 1.9274012E-8 40.0 2 TAGGGCC 220 0.0 39.886364 5 TAAGCGA 40 3.456862E-7 39.375 13 TATGCGG 105 0.0 38.57143 2 TCGATTA 35 6.246435E-6 38.571426 41 GTACGAG 95 0.0 37.894737 1 CCATGCG 30 1.1396803E-4 37.500004 1 GTACGAC 30 1.1396803E-4 37.500004 20 TTAACGG 90 0.0 37.5 2 AGGGTAC 195 0.0 36.92308 6 ATAGGGC 305 0.0 36.885246 4 GCGTAAG 110 0.0 36.81818 1 ACGGGTA 135 0.0 36.666668 5 TAACGGG 265 0.0 36.509434 3 >>END_MODULE