##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554024_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	679762
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.059372250876045	33.0	31.0	34.0	30.0	34.0
2	32.15722708830444	34.0	31.0	34.0	30.0	34.0
3	32.19234084870881	34.0	31.0	34.0	30.0	34.0
4	35.76335246748126	37.0	35.0	37.0	35.0	37.0
5	35.64284705529288	37.0	35.0	37.0	33.0	37.0
6	35.636547791727104	37.0	35.0	37.0	33.0	37.0
7	35.868121489580176	37.0	35.0	37.0	35.0	37.0
8	35.71029272009909	37.0	35.0	37.0	35.0	37.0
9	37.40565080130987	39.0	37.0	39.0	35.0	39.0
10	36.9929357628111	39.0	37.0	39.0	33.0	39.0
11	36.98338241914082	39.0	37.0	39.0	33.0	39.0
12	36.849435537732326	39.0	35.0	39.0	33.0	39.0
13	36.74894742571665	39.0	35.0	39.0	33.0	39.0
14	37.891411994197966	40.0	37.0	41.0	33.0	41.0
15	38.00392931643722	40.0	37.0	41.0	33.0	41.0
16	38.08592124890771	40.0	37.0	41.0	33.0	41.0
17	38.033841256204376	40.0	37.0	41.0	33.0	41.0
18	38.011979192717455	40.0	36.0	41.0	33.0	41.0
19	37.95101373716095	40.0	36.0	41.0	33.0	41.0
20	37.905609610422474	40.0	36.0	41.0	33.0	41.0
21	37.783459504944375	40.0	36.0	41.0	33.0	41.0
22	37.78319470638253	40.0	36.0	41.0	33.0	41.0
23	37.724151982605676	40.0	36.0	41.0	33.0	41.0
24	37.70914525966441	40.0	36.0	41.0	33.0	41.0
25	37.61315872320018	40.0	36.0	41.0	33.0	41.0
26	37.58586976029846	40.0	36.0	41.0	33.0	41.0
27	37.5297707138675	39.0	35.0	41.0	33.0	41.0
28	37.431545158452515	39.0	35.0	41.0	33.0	41.0
29	37.406337806467555	39.0	35.0	41.0	33.0	41.0
30	37.30075526434252	39.0	35.0	41.0	32.0	41.0
31	37.21304515403921	39.0	35.0	41.0	32.0	41.0
32	37.11107564118029	39.0	35.0	41.0	32.0	41.0
33	37.025311800306575	39.0	35.0	41.0	31.0	41.0
34	36.94454382563309	39.0	35.0	41.0	31.0	41.0
35	36.88600863243312	39.0	35.0	41.0	31.0	41.0
36	36.77210700215664	39.0	35.0	41.0	31.0	41.0
37	36.7203153456651	39.0	35.0	41.0	31.0	41.0
38	36.602703593316484	39.0	35.0	41.0	31.0	41.0
39	36.55042647279489	39.0	35.0	41.0	31.0	41.0
40	36.399495705849986	39.0	35.0	40.0	30.0	41.0
41	36.28931008205813	38.0	35.0	40.0	30.0	41.0
42	36.19919618925447	38.0	35.0	40.0	30.0	41.0
43	36.093716624347934	38.0	35.0	40.0	30.0	41.0
44	35.93710445714824	38.0	35.0	40.0	30.0	41.0
45	35.879175358434274	38.0	35.0	40.0	29.0	41.0
46	35.69679534896037	38.0	34.0	40.0	29.0	41.0
47	35.58933567925244	38.0	34.0	40.0	28.0	41.0
48	35.51311194212092	37.0	34.0	40.0	28.0	41.0
49	35.42429556227032	37.0	34.0	40.0	28.0	41.0
50	35.3216993594817	37.0	34.0	40.0	28.0	41.0
51	33.758889140610975	35.0	32.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	6.0
10	7.0
11	4.0
12	5.0
13	7.0
14	19.0
15	37.0
16	49.0
17	106.0
18	202.0
19	406.0
20	652.0
21	1060.0
22	1533.0
23	2120.0
24	2889.0
25	3948.0
26	5007.0
27	6085.0
28	7633.0
29	9641.0
30	12192.0
31	15816.0
32	20614.0
33	28227.0
34	47786.0
35	61236.0
36	57549.0
37	82825.0
38	134722.0
39	177312.0
40	65.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.01422850939005	18.025132325725767	22.427996857723734	15.532642307160446
2	27.485649389050877	26.849985730299725	26.75451113772173	18.90985374292767
3	32.63289210047046	25.806826506924484	25.66971969601125	15.890561696593808
4	28.871722750021334	26.729061053721743	24.22053601113331	20.178680185123618
5	24.94490718810407	30.958482527708224	23.77802819222022	20.31858209196748
6	24.25010518387318	36.61869889755532	23.88100541071728	15.250190507854219
7	81.86482916079451	6.142149752413344	7.508363221245083	4.484657865547059
8	82.68658736440106	5.274934462355942	7.105428076297292	4.933050096945696
9	77.05182107855396	7.351984959441688	9.26559589974138	6.330598062262968
10	39.42026768192397	27.640703658045023	16.87958432510202	16.05944433492899
11	31.960156643060365	27.394293885212768	21.01529653025618	19.63025294147069
12	27.777369138021836	24.224360879248913	27.68277720731668	20.315492775412572
13	23.740662172937	28.170595002368476	28.515274463709357	19.573468360985167
14	21.030007561470043	29.670384634622117	27.208346450669502	22.09126135323834
15	20.03774850609478	27.114784292149313	31.9188186453494	20.928648556406507
16	22.102735957585153	26.096633821837646	30.044486158390733	21.756144062186472
17	21.188151147019106	26.014987598600687	26.97973702560602	25.81712422877419
18	25.364024467387114	23.746840806046823	28.081740373836727	22.80739435272934
19	24.878266216705256	27.971260529420594	24.564332810601357	22.586140443272793
20	27.0390224813979	26.460143403132275	25.21661993462418	21.284214180845648
21	24.36955875732977	28.47849688567469	26.03720125573362	21.11474310126191
22	22.78827001215131	25.45552708153736	25.279288927595246	26.476913978716084
23	22.129068703457975	29.360864537882374	25.247660210485435	23.262406548174212
24	22.642483692821898	25.171898399734026	28.315498659825057	23.87011924761902
25	21.70774476949285	28.405530170853915	26.04411544040414	23.84260961924909
26	21.9069321321286	28.09321497818354	26.457495417513776	23.542357472174086
27	22.728396115110876	27.527428717698253	26.237565500866477	23.50660966632439
28	20.49643845934312	28.68827619078442	27.923596788287664	22.89168856158479
29	22.443443440498292	28.353747340981105	25.520991170439068	23.681818048081535
30	23.764346933191323	27.238504064657924	26.53546388294727	22.461685119203487
31	24.66289671973426	28.631785830923178	23.489544870116305	23.21577257922626
32	24.561243494046444	29.613305833512314	23.84349228112192	21.981958391319314
33	24.237306586717118	28.352570458484	23.092347027341923	24.317775927456964
34	21.859268390995673	28.40714838428744	26.408213462947327	23.32536976176956
35	23.60649756826654	27.564647626669338	24.834721564312215	23.99413324075191
36	23.608557112636483	30.824317923037768	23.441145577422688	22.125979386903065
37	23.13088992912225	29.072234105466325	24.808094597815117	22.988781367596307
38	24.633033326370114	28.938216611108004	24.388683097907798	22.040066964614084
39	25.17792992253171	25.686931602531477	24.76513838667063	24.370000088266185
40	25.59292811307487	24.742189177976996	26.321712599409793	23.343170109538338
41	20.976312297539433	27.469908585652036	25.556150535040207	25.99762858176832
42	24.092697149884813	27.289992673906454	25.45994039090152	23.15736978530721
43	23.8548197751566	25.896416687016927	26.95708203753667	23.29168150028981
44	24.057096454347256	26.392031328612077	26.13856026079716	23.41231195624351
45	24.47385996863608	24.78029074882091	24.87782488576884	25.868024396774164
46	24.680991288127316	25.971148725583365	24.939022775618525	24.408837210670793
47	22.309278835827833	26.792612708565645	27.348542578137643	23.54956587746888
48	23.225040528890993	26.253600524889592	26.843365766253484	23.677993179965927
49	23.36288289136492	25.6619228494679	27.342658165652097	23.632536093515082
50	21.865741244729772	25.850665379941802	28.200458395732625	24.083134979595798
51	22.08228762419788	25.132325725768723	25.39006299263566	27.395323657397736
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1766.0
1	1769.5
2	1773.0
3	1647.5
4	1522.0
5	1243.0
6	964.0
7	1078.5
8	1193.0
9	1225.5
10	1258.0
11	1297.0
12	1336.0
13	1338.5
14	1341.0
15	1275.0
16	1209.0
17	1343.0
18	1477.0
19	1531.0
20	1585.0
21	1826.5
22	2068.0
23	2308.0
24	2548.0
25	3146.0
26	4244.0
27	4744.0
28	6052.0
29	7360.0
30	8464.5
31	9569.0
32	11155.5
33	12742.0
34	14494.0
35	16246.0
36	16918.5
37	17591.0
38	19396.5
39	21202.0
40	24086.5
41	26971.0
42	30457.5
43	33944.0
44	39194.5
45	44445.0
46	49830.0
47	55215.0
48	60623.0
49	66031.0
50	65321.0
51	64611.0
52	58685.5
53	52760.0
54	48705.5
55	44651.0
56	39896.5
57	35142.0
58	33009.5
59	30877.0
60	28976.5
61	27076.0
62	25821.5
63	24567.0
64	21149.0
65	17731.0
66	15491.5
67	13252.0
68	11144.5
69	9037.0
70	8026.5
71	7016.0
72	5902.5
73	4789.0
74	4169.0
75	2972.5
76	2396.0
77	1773.5
78	1151.0
79	916.5
80	682.0
81	491.5
82	301.0
83	242.5
84	184.0
85	129.5
86	75.0
87	45.0
88	15.0
89	15.5
90	16.0
91	12.5
92	9.0
93	11.5
94	14.0
95	15.5
96	17.0
97	8.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	679762.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.257615619322607
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.32889684506712	19.98770883991798
2	10.063939934205772	5.486380126850635
3	3.7331519533458875	3.052704629784766
4	1.8408488726137848	2.0070860393187964
5	1.1472277091576688	1.563534596202788
6	0.825700845229103	1.3503981753482808
7	0.6341571075828631	1.2099927476528567
8	0.5183330302258194	1.1302818000555288
9	0.4216573567340839	1.0344036737625524
>10	6.5824129159957865	43.87793007567066
>50	0.8229979708568526	13.947989508622104
>100	0.07309521451688838	3.6305978532197423
>500	0.005414460334584325	0.9749483503402283
>1k	0.0021657841338337297	0.7460435832530812
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGC	1624	0.23890714691318432	No Hit
CCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGC	1383	0.20345356168776718	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	1029	0.15137651119068143	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1019	0.14990540806929487	No Hit
CTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGCT	962	0.14152012027739122	Illumina Single End Adapter 1 (95% over 21bp)
TATATATATATATATATATATATATATATATATATATATATATATATATAT	799	0.11754113939878959	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCC	709	0.10430121130631015	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.14975829775715618	0.0
2	0.0	0.0	0.0	0.8004272083464506	0.0
3	0.0	0.0	0.0	1.099502472924347	0.0
4	0.0	0.0	0.0	1.4943465507045113	0.0
5	0.0	0.0	0.0	2.700503999929387	0.0
6	0.0	0.0	0.0	3.610381280507001	0.0
7	0.0	0.0	0.0	4.441260323466154	0.0
8	0.0	0.0	0.0	5.723768024690995	0.0
9	0.0	0.0	0.0	6.245568301846823	0.0
10	0.0	0.0	0.0	7.37140352064399	0.0
11	0.0	0.0	0.0	8.6484681403197	0.0
12	0.0	0.0	0.0	9.712811248642907	0.0
13	0.0	0.0	0.0	10.134429403232307	0.0
14	0.0	0.0	0.0	10.315080866538583	0.0
15	0.0	0.0	0.0	10.63710533981011	0.0
16	0.0	0.0	0.0	11.316166540642167	0.0
17	0.0	0.0	0.0	12.109091123069545	0.0
18	0.0	0.0	0.0	13.063248607600896	0.0
19	0.0	0.0	0.0	13.532971834259637	0.0
20	0.0	0.0	0.0	13.989013801889485	0.0
21	0.0	0.0	0.0	14.515962939970166	0.0
22	0.0	0.0	0.0	15.049532042097088	0.0
23	0.0	0.0	0.0	15.632971540039014	0.0
24	0.0	0.0	0.0	16.075626469264243	0.0
25	0.0	0.0	0.0	16.498421506350752	0.0
26	0.0	0.0	0.0	16.853545799853478	0.0
27	0.0	0.0	0.0	17.235297059853302	0.0
28	0.0	0.0	0.0	17.60969280424619	0.0
29	0.0	0.0	0.0	17.98467698988764	0.0
30	0.0	0.0	0.0	18.439689185332515	0.0
31	0.0	0.0	0.0	18.823647100014416	0.0
32	0.0	0.0	0.0	19.21496053030325	0.0
33	0.0	0.0	0.0	19.614659248383994	0.0
34	0.0	0.0	0.0	19.96198669534337	0.0
35	0.0	0.0	0.0	20.378455989007918	0.0
36	0.0	0.0	0.0	20.74932108590948	0.0
37	0.0	0.0	0.0	21.14063451619832	0.0
38	0.0	0.0	0.0	21.503261435620114	0.0
39	0.0	0.0	0.0	21.879863834695083	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGATAG	30	2.1641881E-6	45.000004	1
TAGCCCG	30	2.1641881E-6	45.000004	1
TCGTTTA	20	7.03125E-4	45.0	38
TCGTTGA	20	7.03125E-4	45.0	24
CTCCGAC	20	7.03125E-4	45.0	30
GTCTACG	25	3.889118E-5	45.0	1
ACGATTG	20	7.03125E-4	45.0	1
TCTACGG	35	1.2109558E-7	45.0	2
GCTTAAC	20	7.03125E-4	45.0	15
GATATGC	20	7.03125E-4	45.0	11
TATTACG	25	3.889118E-5	45.0	1
TCGTAAG	25	3.889118E-5	45.0	1
TTACGAA	20	7.03125E-4	45.0	45
CGCACGG	40	6.8084773E-9	45.0	2
CACGTTA	20	7.03125E-4	45.0	44
ATCGGTA	25	3.889118E-5	45.0	41
ATACGAG	20	7.03125E-4	45.0	1
ATGCCTA	20	7.03125E-4	45.0	38
TACGTGA	20	7.03125E-4	45.0	42
TAGTACG	20	7.03125E-4	45.0	1
>>END_MODULE