FastQCFastQC Report
Sat 18 Jun 2016
SRR3554023_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554023_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences666340
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCTGC15000.22511030404898402No Hit
GCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCTGC14140.21220397995017562No Hit
CTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCTGCT9820.1473722123840682No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG7200.10805294594351232No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA6980.10475132815079388No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATGC453.8380676E-1045.00000442
TCGCGCA453.8380676E-1045.00000434
TCGTTTA207.031151E-445.038
TTTACGT207.031151E-445.031
ACGCATA253.8890343E-545.015
CTATACG207.031151E-445.01
CGTAGAA207.031151E-445.036
CTTGACG351.2109012E-745.01
TGTTACG207.031151E-445.01
CGGTCTA207.031151E-445.031
TTACCGG351.2109012E-745.02
TAATGCG351.2109012E-745.01
TACCGGC207.031151E-445.011
CCCGATT207.031151E-445.024
CGCTACA700.041.78571328
AATACGG556.002665E-1140.9090922
GTCGATG501.0804797E-940.51
CGTGTAC451.9261279E-840.00000418
AACACGT2200.039.88636441
AATAGCG403.4552977E-739.3751