##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554023_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	666340
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.065925803643786	33.0	31.0	34.0	30.0	34.0
2	32.181549959480144	34.0	31.0	34.0	30.0	34.0
3	32.202590269231926	34.0	31.0	34.0	30.0	34.0
4	35.77320737161209	37.0	35.0	37.0	35.0	37.0
5	35.65043221178377	37.0	35.0	37.0	33.0	37.0
6	35.641298736380826	37.0	35.0	37.0	33.0	37.0
7	35.856718792208184	37.0	35.0	37.0	35.0	37.0
8	35.727927184320315	37.0	35.0	37.0	35.0	37.0
9	37.41051565266981	39.0	37.0	39.0	35.0	39.0
10	37.00824504006963	39.0	37.0	39.0	33.0	39.0
11	36.979378395413754	39.0	37.0	39.0	33.0	39.0
12	36.85337965603146	39.0	35.0	39.0	33.0	39.0
13	36.805134015667676	39.0	35.0	39.0	33.0	39.0
14	37.90913647687367	40.0	37.0	41.0	33.0	41.0
15	38.03863643185161	40.0	37.0	41.0	33.0	41.0
16	38.10190443317225	40.0	37.0	41.0	33.0	41.0
17	38.06125401446709	40.0	37.0	41.0	33.0	41.0
18	38.02944592850497	40.0	37.0	41.0	33.0	41.0
19	37.992661404088004	40.0	37.0	41.0	34.0	41.0
20	37.95880031215295	40.0	36.0	41.0	33.0	41.0
21	37.82095176636552	40.0	36.0	41.0	33.0	41.0
22	37.806648257646245	40.0	36.0	41.0	33.0	41.0
23	37.765703694810455	40.0	36.0	41.0	33.0	41.0
24	37.73920370981781	40.0	36.0	41.0	33.0	41.0
25	37.63392412282018	40.0	36.0	41.0	33.0	41.0
26	37.603013476603536	40.0	36.0	41.0	33.0	41.0
27	37.551751358165504	40.0	36.0	41.0	33.0	41.0
28	37.443488309271544	39.0	35.0	41.0	33.0	41.0
29	37.43109523666597	39.0	35.0	41.0	33.0	41.0
30	37.32372662604676	39.0	35.0	41.0	32.0	41.0
31	37.19592400276135	39.0	35.0	41.0	32.0	41.0
32	37.08500315154426	39.0	35.0	41.0	31.0	41.0
33	37.01997028543987	39.0	35.0	41.0	31.0	41.0
34	36.91476573521025	39.0	35.0	41.0	31.0	41.0
35	36.84554431671519	39.0	35.0	41.0	31.0	41.0
36	36.70889785995138	39.0	35.0	41.0	31.0	41.0
37	36.63867545097098	39.0	35.0	41.0	31.0	41.0
38	36.516650658822826	39.0	35.0	41.0	30.0	41.0
39	36.4927409430621	39.0	35.0	41.0	30.0	41.0
40	36.365106402137044	39.0	35.0	40.0	30.0	41.0
41	36.25990035117207	38.0	35.0	40.0	30.0	41.0
42	36.14441426298887	38.0	35.0	40.0	30.0	41.0
43	36.027365909295554	38.0	35.0	40.0	30.0	41.0
44	35.89795599843924	38.0	35.0	40.0	29.0	41.0
45	35.85430410901342	38.0	35.0	40.0	29.0	41.0
46	35.72530389891047	38.0	34.0	40.0	29.0	41.0
47	35.59685595942012	38.0	34.0	40.0	28.0	41.0
48	35.495208151994476	37.0	34.0	40.0	28.0	41.0
49	35.41399435723505	37.0	34.0	40.0	28.0	41.0
50	35.29582195275685	37.0	34.0	40.0	28.0	41.0
51	33.74782993666897	35.0	32.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	5.0
10	4.0
11	4.0
12	9.0
13	10.0
14	18.0
15	30.0
16	56.0
17	89.0
18	229.0
19	378.0
20	657.0
21	927.0
22	1379.0
23	2186.0
24	2764.0
25	3881.0
26	4780.0
27	6113.0
28	7547.0
29	9700.0
30	12044.0
31	15479.0
32	20078.0
33	27677.0
34	47009.0
35	59000.0
36	57334.0
37	82062.0
38	131357.0
39	173466.0
40	67.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.837890566377524	18.15949815409551	23.510820301947955	15.491790977579015
2	28.37605426659063	25.51580274334424	28.09346579824114	18.014677191823996
3	32.83008674250383	25.07563706216046	26.098088063150943	15.996188132184772
4	28.070954767836238	26.952306630248824	25.49284149233124	19.483897109583694
5	24.63907314584146	30.515952816880272	24.974337425338415	19.87063661193985
6	24.216015847765405	36.43830476933697	24.071945253174054	15.273734129723564
7	82.37341297235645	6.2628688057148	7.115586637452352	4.248131584476393
8	84.49830416904283	4.61130954167542	6.243509319566588	4.64687696971516
9	78.47360206501186	7.02119038328781	8.59351082030195	5.9116967313983855
10	41.12390071134856	25.942611879821115	16.110994387249754	16.822493021580577
11	31.81784074196356	26.59408109973887	21.93309721763664	19.654980940660923
12	27.48341687426839	23.43368250442717	28.478704565236967	20.604196056067472
13	24.61070924753129	25.960320557072965	29.15973827175316	20.269231923642586
14	21.139358285559926	29.09085451871417	28.24323918720173	21.526548008524177
15	19.804304109013415	27.010084941621393	32.27541495332713	20.91019599603806
16	23.2646997028544	24.277245850466727	31.069273944232673	21.3887805024462
17	22.404027973707116	25.48383708016928	27.155206050964974	24.95692889515863
18	23.8111174475493	25.078488459345078	28.49551280127262	22.614881291832997
19	24.64732719032326	26.82279316865264	25.52180568478554	23.008073956238555
20	26.483026683074705	25.752168562595674	25.457274064291504	22.307530690038117
21	25.34907104481196	27.739742473812168	25.829456433652492	21.081730047723386
22	23.48290662424588	25.27163310021911	25.820301947954498	25.425158327580515
23	22.785965122910227	27.28742083620974	25.835909595701896	24.090704445178137
24	22.428790107152505	25.842062610679235	28.50946964012366	23.2196776420446
25	23.13158447639343	26.82219287450851	26.299186601434705	23.747036047663357
26	22.224389951076027	27.0827205330612	26.633700513251497	24.05918900261128
27	23.54098508269052	26.396284179247832	26.67136897079569	23.391361767265963
28	21.11819791697932	27.519884743524326	28.653390161178976	22.708527178317375
29	23.404118017828736	26.509739772488523	26.492631389380794	23.593510820301947
30	25.014106912387067	25.92445298196116	26.608488159198007	22.452951946453762
31	26.639103160548665	26.997628838130684	24.002761353063	22.360506648257648
32	25.346669868235434	28.065251973467	24.67704175045772	21.91103640783984
33	26.336554911906834	26.903832878110272	23.42392772458505	23.335684485397845
34	23.91556862862803	26.286730497943996	26.65215955818351	23.14554131524447
35	25.299396704385146	26.382327340396795	25.178437434342825	23.139838520875227
36	26.68667647147102	28.058498664345528	25.164030374883694	20.090794489299757
37	25.030314854278597	28.461145961521144	25.71314944322718	20.79538974097308
38	23.488909565687184	30.116306990425308	24.774139328270852	21.620644115616653
39	25.191944052585768	27.40147672359456	24.971936248761896	22.434642975057777
40	25.536362817780713	25.973076807635742	26.595881982171264	21.89467839241228
41	23.080259327070266	26.948104571239906	25.633010174985742	24.338625926704086
42	23.56649758381607	27.090824504006967	25.650118558093464	23.6925593540835
43	24.22231893627878	25.131014196956507	26.89617912777261	23.750487738992106
44	23.90941561365069	25.446168622625088	26.599933967644144	24.04448179608008
45	24.666236455863373	25.39379295854969	25.86592430290843	24.074046282678513
46	25.25077287871057	26.51934447879461	25.80004202059009	22.429840621904734
47	23.518624125821653	26.49023021280427	27.283668997808924	22.707476663565146
48	22.268511570669627	26.465918299966983	27.277816129903655	23.987753999459734
49	23.50601794879491	25.536963111924845	28.029234324819164	22.927784614461086
50	22.873007773809167	24.924663084911607	28.242338745985535	23.959990395293694
51	22.529789596902482	25.107752798871445	26.17732689017619	26.185130714049887
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1457.0
1	1492.0
2	1527.0
3	1472.0
4	1417.0
5	1121.5
6	826.0
7	969.0
8	1112.0
9	1187.0
10	1262.0
11	1272.0
12	1282.0
13	1298.5
14	1315.0
15	1242.5
16	1170.0
17	1236.0
18	1302.0
19	1415.5
20	1529.0
21	1760.5
22	1992.0
23	1951.0
24	1910.0
25	2910.5
26	4548.5
27	5186.0
28	6718.5
29	8251.0
30	9290.0
31	10329.0
32	11569.0
33	12809.0
34	14039.0
35	15269.0
36	16890.0
37	18511.0
38	18988.5
39	19466.0
40	24319.0
41	29172.0
42	31322.0
43	33472.0
44	38412.5
45	43353.0
46	47537.5
47	51722.0
48	56901.0
49	62080.0
50	61191.0
51	60302.0
52	54735.5
53	49169.0
54	44064.5
55	38960.0
56	36808.5
57	34657.0
58	33738.5
59	32820.0
60	31105.5
61	29391.0
62	26830.0
63	24269.0
64	21798.0
65	19327.0
66	17062.5
67	14798.0
68	12344.5
69	9891.0
70	8868.5
71	7846.0
72	6455.0
73	5064.0
74	4341.5
75	2904.5
76	2190.0
77	1604.5
78	1019.0
79	797.0
80	575.0
81	495.5
82	416.0
83	308.0
84	200.0
85	139.5
86	79.0
87	56.0
88	33.0
89	35.0
90	37.0
91	26.5
92	16.0
93	18.5
94	21.0
95	14.0
96	7.0
97	4.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	666340.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.51171338587282
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.21823013382932	20.14358962062789
2	9.731925197973931	5.354838734788248
3	3.5096985031974706	2.896734578723869
4	1.7609701701135034	1.9378922640493763
5	1.1163432970277452	1.5356258414033805
6	0.8433815356865139	1.3921722650846782
7	0.6434078415576374	1.2390876489009757
8	0.5702362231539011	1.2550540426922192
9	0.4707085285089151	1.1654998312160894
>10	7.51633323236216	49.834356660879955
>50	0.563958840876881	9.423727978650307
>100	0.04932584614083337	2.584706075457508
>500	0.004384519656962967	0.7973355341861686
>1k	0.0010961299142407416	0.43937892333935236
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCTGC	1500	0.22511030404898402	No Hit
GCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCTGC	1414	0.21220397995017562	No Hit
CTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCTGCT	982	0.1473722123840682	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	720	0.10805294594351232	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	698	0.10475132815079388	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.15352522736140709	0.0
2	0.0	0.0	0.0	0.759522165861272	0.0
3	0.0	0.0	0.0	1.001290632409881	0.0
4	0.0	0.0	0.0	1.3394063090914547	0.0
5	0.0	0.0	0.0	2.2806675270882732	0.0
6	0.0	0.0	0.0	3.1113245490290242	0.0
7	0.0	0.0	0.0	3.8372302428189813	0.0
8	0.0	0.0	0.0	5.057328090764474	0.0
9	0.0	0.0	0.0	5.588438334784044	0.0
10	0.0	0.0	0.0	6.631899630819102	0.0
11	0.0	0.0	0.0	7.81898130083741	0.0
12	0.0	0.0	0.0	8.772698622324938	0.0
13	0.0	0.0	0.0	9.147132094726416	0.0
14	0.0	0.0	0.0	9.302608278056248	0.0
15	0.0	0.0	0.0	9.565387039649428	0.0
16	0.0	0.0	0.0	10.145871477023741	0.0
17	0.0	0.0	0.0	10.851217096377225	0.0
18	0.0	0.0	0.0	11.66461566167422	0.0
19	0.0	0.0	0.0	12.071314944322719	0.0
20	0.0	0.0	0.0	12.485818050844914	0.0
21	0.0	0.0	0.0	12.951496233154245	0.0
22	0.0	0.0	0.0	13.448839931566468	0.0
23	0.0	0.0	0.0	13.935678482456403	0.0
24	0.0	0.0	0.0	14.348080559474143	0.0
25	1.50073536032656E-4	0.0	0.0	14.703604766335504	0.0
26	1.50073536032656E-4	0.0	0.0	15.047273163850287	0.0
27	1.50073536032656E-4	0.0	0.0	15.389740973076808	0.0
28	1.50073536032656E-4	0.0	0.0	15.749917459555181	0.0
29	1.50073536032656E-4	0.0	0.0	16.100939460335564	0.0
30	1.50073536032656E-4	0.0	0.0	16.524146831947654	0.0
31	3.00147072065312E-4	0.0	0.0	16.86916589128673	0.0
32	3.00147072065312E-4	0.0	0.0	17.23984752528739	0.0
33	3.00147072065312E-4	0.0	0.0	17.594471290932557	0.0
34	3.00147072065312E-4	0.0	0.0	17.93588858540685	0.0
35	3.00147072065312E-4	0.0	0.0	18.320977278866646	0.0
36	3.00147072065312E-4	0.0	0.0	18.673650088543386	0.0
37	3.00147072065312E-4	0.0	0.0	19.0183690008104	0.0
38	3.00147072065312E-4	0.0	0.0	19.366989825014258	0.0
39	4.50220608097968E-4	0.0	0.0	19.708407119488548	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGATGC	45	3.8380676E-10	45.000004	42
TCGCGCA	45	3.8380676E-10	45.000004	34
TCGTTTA	20	7.031151E-4	45.0	38
TTTACGT	20	7.031151E-4	45.0	31
ACGCATA	25	3.8890343E-5	45.0	15
CTATACG	20	7.031151E-4	45.0	1
CGTAGAA	20	7.031151E-4	45.0	36
CTTGACG	35	1.2109012E-7	45.0	1
TGTTACG	20	7.031151E-4	45.0	1
CGGTCTA	20	7.031151E-4	45.0	31
TTACCGG	35	1.2109012E-7	45.0	2
TAATGCG	35	1.2109012E-7	45.0	1
TACCGGC	20	7.031151E-4	45.0	11
CCCGATT	20	7.031151E-4	45.0	24
CGCTACA	70	0.0	41.785713	28
AATACGG	55	6.002665E-11	40.909092	2
GTCGATG	50	1.0804797E-9	40.5	1
CGTGTAC	45	1.9261279E-8	40.000004	18
AACACGT	220	0.0	39.886364	41
AATAGCG	40	3.4552977E-7	39.375	1
>>END_MODULE