SRR3554022_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3554022_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1155013
Sequences flagged as poor quality	0
Sequence length	51
%GC	50

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTGC	2140	0.18527929988666794	Illumina PCR Primer Index 8 (95% over 23bp)
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	1621	0.14034474070854613	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1615	0.13982526603596668	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	1419	0.12285576006503822	No Hit
CTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTGCT	1313	0.11367837418280141	RNA PCR Primer, Index 40 (96% over 26bp)
CCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTGC	1207	0.10450098830056458	RNA PCR Primer, Index 40 (96% over 25bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTAACG	25	3.890817E-5	45.000004	1
AATTCGC	25	3.890817E-5	45.000004	13
CAATGCG	35	1.211938E-7	45.000004	1
ACGGTAC	25	3.890817E-5	45.000004	13
ACGTTCA	20	7.0333015E-4	45.0	17
GCTATCG	20	7.0333015E-4	45.0	1
ATCGTAG	20	7.0333015E-4	45.0	1
ACGATAC	20	7.0333015E-4	45.0	31
TCATACG	40	6.8157533E-9	45.0	1
ATACGTA	20	7.0333015E-4	45.0	40
CCGCATT	20	7.0333015E-4	45.0	13
TCGTTAC	30	2.1655105E-6	44.999996	35
CATACGA	290	0.0	41.89655	18
TACGAAT	320	0.0	41.484375	12
TCTAGCG	50	1.0822987E-9	40.500004	1
CTATACG	45	1.9283107E-8	40.0	1
GTTCACG	45	1.9283107E-8	40.0	1
GCTACGA	340	0.0	39.705883	10
ACGTTAG	40	3.4582445E-7	39.375	1
CGGTCTA	40	3.4582445E-7	39.375	31