##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554022_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1155013
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.094533135124884	33.0	31.0	34.0	30.0	34.0
2	32.210218413126086	34.0	31.0	34.0	30.0	34.0
3	32.24484053426239	34.0	31.0	34.0	30.0	34.0
4	35.795754679817456	37.0	35.0	37.0	35.0	37.0
5	35.67233615552379	37.0	35.0	37.0	33.0	37.0
6	35.66647994438158	37.0	35.0	37.0	33.0	37.0
7	35.872828271196944	37.0	35.0	37.0	35.0	37.0
8	35.72117976161307	37.0	35.0	37.0	35.0	37.0
9	37.41356157896058	39.0	37.0	39.0	35.0	39.0
10	37.031379733388285	39.0	37.0	39.0	33.0	39.0
11	36.98949275895596	39.0	37.0	39.0	33.0	39.0
12	36.88655452362874	39.0	37.0	39.0	33.0	39.0
13	36.85216443451286	39.0	37.0	39.0	33.0	39.0
14	37.98576985713581	40.0	37.0	41.0	33.0	41.0
15	38.10577889599511	40.0	37.0	41.0	33.0	41.0
16	38.153630305459764	40.0	37.0	41.0	33.0	41.0
17	38.118236764434684	40.0	37.0	41.0	33.0	41.0
18	38.09271151060637	40.0	37.0	41.0	33.0	41.0
19	38.043009905516215	40.0	37.0	41.0	34.0	41.0
20	37.97251286349158	40.0	36.0	41.0	33.0	41.0
21	37.86449156849317	40.0	36.0	41.0	33.0	41.0
22	37.860616287435725	40.0	36.0	41.0	33.0	41.0
23	37.806514731868816	40.0	36.0	41.0	33.0	41.0
24	37.79585338000525	40.0	36.0	41.0	33.0	41.0
25	37.678337819574324	40.0	36.0	41.0	33.0	41.0
26	37.630663897289466	40.0	36.0	41.0	33.0	41.0
27	37.570749420136394	40.0	36.0	41.0	33.0	41.0
28	37.47003886536342	40.0	36.0	41.0	33.0	41.0
29	37.438160436289465	39.0	36.0	41.0	33.0	41.0
30	37.319968692993065	39.0	35.0	41.0	32.0	41.0
31	37.24433924120335	39.0	35.0	41.0	32.0	41.0
32	37.14238367879842	39.0	35.0	41.0	32.0	41.0
33	37.04608346399564	39.0	35.0	41.0	31.0	41.0
34	36.96085325446553	39.0	35.0	41.0	31.0	41.0
35	36.90637334817877	39.0	35.0	41.0	31.0	41.0
36	36.7947694095218	39.0	35.0	41.0	31.0	41.0
37	36.73707741817625	39.0	35.0	41.0	31.0	41.0
38	36.641967666164796	39.0	35.0	41.0	31.0	41.0
39	36.56633301962835	39.0	35.0	41.0	30.0	41.0
40	36.418080142820905	39.0	35.0	41.0	30.0	41.0
41	36.31535402631832	39.0	35.0	40.0	30.0	41.0
42	36.21755599287627	38.0	35.0	40.0	30.0	41.0
43	36.11047494703523	38.0	35.0	40.0	30.0	41.0
44	35.98007468314209	38.0	35.0	40.0	29.0	41.0
45	35.89314146247705	38.0	35.0	40.0	29.0	41.0
46	35.73478653487017	38.0	35.0	40.0	29.0	41.0
47	35.650250689819075	38.0	34.0	40.0	29.0	41.0
48	35.55560413605734	38.0	34.0	40.0	28.0	41.0
49	35.4573480991123	37.0	34.0	40.0	28.0	41.0
50	35.369865101085445	37.0	34.0	40.0	28.0	41.0
51	33.76905021848239	36.0	32.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	6.0
10	7.0
11	13.0
12	12.0
13	14.0
14	32.0
15	53.0
16	102.0
17	181.0
18	340.0
19	681.0
20	1111.0
21	1642.0
22	2549.0
23	3593.0
24	4901.0
25	6354.0
26	8408.0
27	10692.0
28	13062.0
29	16274.0
30	20854.0
31	26646.0
32	34395.0
33	47260.0
34	78720.0
35	99411.0
36	98231.0
37	139932.0
38	226227.0
39	313186.0
40	124.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.57492080175721	17.571923432896426	23.303374074577516	15.54978169076885
2	27.940118422909528	25.59373790598028	27.586702487331312	18.879441183778884
3	33.07278792533071	24.77314108152895	25.469929775682175	16.68414121745816
4	28.39942061258185	26.859264787495896	25.27045150141167	19.47086309851058
5	25.026904459084008	30.853505544959237	24.173061255587598	19.946528740369157
6	24.964827235710768	36.80893634963416	23.326577276619396	14.899659138035675
7	82.64210013220631	6.252224000941981	6.675682438206324	4.429993428645392
8	83.9685787086379	5.169379045950132	6.22469184329527	4.637350402116686
9	77.92466405140028	7.6399140096258655	8.667088595539617	5.76833334343423
10	41.30654806482698	26.37424860153089	15.878349421175347	16.440853912466785
11	31.779555727944185	26.52974468685634	21.896203765671903	19.794495819527572
12	28.029987541265772	23.874276739742324	27.569992718696675	20.525743000295236
13	24.653748485947776	25.460665810687843	29.02962996953281	20.855955733831564
14	21.409109681016577	28.18660915504847	27.6880000484843	22.71628111545065
15	21.117078335914837	25.755900582937162	31.61297751627038	21.514043564877625
16	24.108906133524037	24.458685746394195	29.56191835070255	21.87048976937922
17	23.44735513799412	24.820586434957875	26.61441905848679	25.117639368561218
18	25.020757342125155	25.154868386762747	27.2205594222749	22.6038148488372
19	25.791571177120947	26.980302386206905	24.892273939773837	22.335852496898305
20	26.944978108471506	25.72940737463561	25.226729049802906	22.098885467089982
21	25.92905880713031	27.48142228702188	25.324043971799455	21.265474934048363
22	24.14604857261347	24.91426503424637	25.54300254629169	25.39668384684848
23	23.332204918905674	27.018830091089885	25.55122756194086	24.09773742806358
24	23.16276959653268	26.110095730524247	27.61882333791914	23.108311335023934
25	23.65471211146541	26.94939364318843	25.710100232638073	23.68579401270808
26	22.58840376688401	27.7286056520576	25.580318143605307	24.102672437453084
27	23.80276239315055	26.242734930256194	25.83157072690957	24.122931949683682
28	21.865727918213906	27.729038547618078	26.642037795245592	23.76319573892242
29	23.089696825923173	27.460643300118697	25.241187761523033	24.208472112435096
30	24.08682845993941	27.360904162983445	24.968203821082533	23.584063555994607
31	24.838854627610253	27.99111351993441	22.386068381914317	24.783963470541025
32	24.2261342513028	28.84183987539534	23.755663356170018	23.17636251713184
33	24.121893000338524	27.58826091134905	23.17160066596653	25.118245422345897
34	22.025985854704665	28.500112119950167	24.889330249962555	24.584571775382617
35	23.392290822700698	26.078321196384803	25.20127479084651	25.32811319006799
36	22.9247636173792	30.105202279108546	23.471250972932772	23.498783130579483
37	23.120259252493263	28.578293058173372	24.515308485705354	23.78613920362801
38	23.144414824768205	28.644699237151443	24.571844645904417	23.639041292175932
39	23.77323891592562	26.567233442394155	24.655480068189707	25.004047573490514
40	24.069858953968485	25.646551164359188	26.778659634133987	23.50493024753834
41	21.05707901123191	25.97970758770681	26.550263936423224	26.412949464638057
42	22.45524509247948	26.17260584945797	25.86689500464497	25.50525405341758
43	22.802081015538352	25.50663931921113	26.158926349746714	25.53235331550381
44	23.274370072025164	26.012607650303504	26.03139531762846	24.681626960042873
45	23.78345525115302	25.576162346224674	25.30179313999063	25.33858926263168
46	22.80190785731416	27.29865378138601	25.207075591356983	24.69236276994285
47	22.637753860779057	26.147238169613672	27.16142588871294	24.05358208089433
48	21.92460171443958	25.75442873803152	26.875887977018444	25.445081570510464
49	21.952047292974193	25.692264935546177	27.73561856013742	24.620069211342212
50	21.475689018218844	25.478847424228125	27.66713448246903	25.378329075084004
51	21.496121688673632	25.4542589563927	26.115896531034714	26.933722823898954
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1868.0
1	2031.0
2	2194.0
3	2362.5
4	2531.0
5	1992.5
6	1454.0
7	1582.0
8	1710.0
9	1819.5
10	1929.0
11	2021.5
12	2114.0
13	2125.0
14	2136.0
15	2160.0
16	2184.0
17	2077.0
18	1970.0
19	2250.5
20	2531.0
21	2672.5
22	2814.0
23	3373.0
24	3932.0
25	5544.0
26	7794.5
27	8433.0
28	11506.5
29	14580.0
30	15457.0
31	16334.0
32	19519.0
33	22704.0
34	25499.5
35	28295.0
36	30387.5
37	32480.0
38	34403.0
39	36326.0
40	41666.0
41	47006.0
42	50678.5
43	54351.0
44	61871.5
45	69392.0
46	77339.5
47	85287.0
48	95353.5
49	105420.0
50	100803.5
51	96187.0
52	89732.5
53	83278.0
54	77052.0
55	70826.0
56	66551.0
57	62276.0
58	60649.5
59	59023.0
60	55428.5
61	51834.0
62	49117.0
63	46400.0
64	41687.5
65	36975.0
66	31523.5
67	26072.0
68	23003.5
69	19935.0
70	18231.0
71	16527.0
72	13649.5
73	10772.0
74	9035.0
75	5943.0
76	4588.0
77	3568.0
78	2548.0
79	2144.0
80	1740.0
81	1340.5
82	941.0
83	651.0
84	361.0
85	255.5
86	150.0
87	103.0
88	56.0
89	49.0
90	42.0
91	34.5
92	27.0
93	22.5
94	18.0
95	13.0
96	8.0
97	4.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1155013.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.951685222001153
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.43449448301867	18.32314390777062
2	9.725144178538107	4.853174725629197
3	3.68300379282026	2.7569145392966243
4	1.8177895048925055	1.814276461037405
5	1.1652090144585534	1.4536964273304003
6	0.8178265177406019	1.2243689902121304
7	0.6076258847658598	1.0612902866592377
8	0.529731656402184	1.0574158034141254
9	0.4093303260891616	0.9192133303556261
>10	6.402963482559028	39.96420685904404
>50	1.3134840691003764	21.051389533337648
>100	0.08573016434781143	3.6905770195213403
>500	0.004878952442557332	0.8339058860846587
>1k	0.002787972824318475	0.9964262303069973
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTGC	2140	0.18527929988666794	Illumina PCR Primer Index 8 (95% over 23bp)
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	1621	0.14034474070854613	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1615	0.13982526603596668	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	1419	0.12285576006503822	No Hit
CTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTGCT	1313	0.11367837418280141	RNA PCR Primer, Index 40 (96% over 26bp)
CCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTGC	1207	0.10450098830056458	RNA PCR Primer, Index 40 (96% over 25bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.65791120965738E-5	0.0	0.0	0.11921943735698214	0.0
2	8.65791120965738E-5	0.0	0.0	0.5463141973293807	0.0
3	8.65791120965738E-5	0.0	0.0	0.7553161739305099	0.0
4	8.65791120965738E-5	0.0	0.0	1.0507241044040196	0.0
5	8.65791120965738E-5	0.0	0.0	1.8888099094988542	0.0
6	8.65791120965738E-5	0.0	0.0	2.702220667646165	0.0
7	8.65791120965738E-5	0.0	0.0	3.3400489864616243	0.0
8	8.65791120965738E-5	0.0	0.0	4.433456593129255	0.0
9	8.65791120965738E-5	0.0	0.0	4.908343022978962	0.0
10	8.65791120965738E-5	0.0	0.0	5.816904225320408	0.0
11	8.65791120965738E-5	0.0	0.0	6.788408442156062	0.0
12	8.65791120965738E-5	0.0	0.0	7.574719938217146	0.0
13	1.731582241931476E-4	0.0	0.0	7.900430557924456	0.0
14	1.731582241931476E-4	0.0	0.0	8.032290545647538	0.0
15	1.731582241931476E-4	0.0	0.0	8.26423598695426	0.0
16	1.731582241931476E-4	0.0	0.0	8.754620077869253	0.0
17	1.731582241931476E-4	0.0	0.0	9.362578603011395	0.0
18	1.731582241931476E-4	0.0	0.0	10.048458329040452	0.0
19	1.731582241931476E-4	0.0	0.0	10.406376378447689	0.0
20	1.731582241931476E-4	0.0	0.0	10.770008649253299	0.0
21	1.731582241931476E-4	0.0	0.0	11.209484222255506	0.0
22	1.731582241931476E-4	0.0	0.0	11.659522446933497	0.0
23	1.731582241931476E-4	0.0	0.0	12.124192541555809	0.0
24	2.597373362897214E-4	0.0	0.0	12.516828814913772	0.0
25	2.597373362897214E-4	0.0	0.0	12.865915794887156	0.0
26	2.597373362897214E-4	0.0	0.0	13.175695857968698	0.0
27	2.597373362897214E-4	0.0	0.0	13.488159873525234	0.0
28	2.597373362897214E-4	0.0	0.0	13.82434656579623	0.0
29	2.597373362897214E-4	0.0	0.0	14.154299561996272	0.0
30	2.597373362897214E-4	0.0	0.0	14.542260563301019	0.0
31	2.597373362897214E-4	0.0	0.0	14.88338226496152	0.0
32	2.597373362897214E-4	0.0	0.0	15.241127156144563	0.0
33	4.3289556048286903E-4	0.0	0.0	15.586837550746182	0.0
34	5.194746725794428E-4	0.0	0.0	15.926573986613137	0.0
35	5.194746725794428E-4	0.0	0.0	16.30224075400017	0.0
36	6.060537846760166E-4	0.0	0.0	16.65652248069935	0.0
37	6.060537846760166E-4	0.0	0.0	16.999029448153397	0.0
38	6.060537846760166E-4	0.0	0.0	17.360930136717077	0.0
39	6.060537846760166E-4	0.0	0.0	17.713653439398517	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTAACG	25	3.890817E-5	45.000004	1
AATTCGC	25	3.890817E-5	45.000004	13
CAATGCG	35	1.211938E-7	45.000004	1
ACGGTAC	25	3.890817E-5	45.000004	13
ACGTTCA	20	7.0333015E-4	45.0	17
GCTATCG	20	7.0333015E-4	45.0	1
ATCGTAG	20	7.0333015E-4	45.0	1
ACGATAC	20	7.0333015E-4	45.0	31
TCATACG	40	6.8157533E-9	45.0	1
ATACGTA	20	7.0333015E-4	45.0	40
CCGCATT	20	7.0333015E-4	45.0	13
TCGTTAC	30	2.1655105E-6	44.999996	35
CATACGA	290	0.0	41.89655	18
TACGAAT	320	0.0	41.484375	12
TCTAGCG	50	1.0822987E-9	40.500004	1
CTATACG	45	1.9283107E-8	40.0	1
GTTCACG	45	1.9283107E-8	40.0	1
GCTACGA	340	0.0	39.705883	10
ACGTTAG	40	3.4582445E-7	39.375	1
CGGTCTA	40	3.4582445E-7	39.375	31
>>END_MODULE