FastQCFastQC Report
Sat 18 Jun 2016
SRR3554021_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554021_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1159077
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGC21360.18428456435594875Illumina PCR Primer Index 11 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGC21310.18385318663039643Illumina PCR Primer Index 11 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGCT15490.13364081937610703Illumina PCR Primer Index 11 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAA207.03331E-445.00000424
CGGATTA302.1655178E-645.00000414
ACTACGC207.03331E-445.00000413
CTGCGTA253.8908267E-545.01
CATACGA850.042.3529418
CCGTATA650.041.5384633
CACGACC5600.041.38392627
CTAACGG603.6379788E-1241.2500042
CCGTTAG556.184564E-1140.9090921
GTACGAG556.184564E-1140.9090921
AACACGT5500.040.90909241
ACACGAC5700.040.26315726
TATAACG451.9283107E-840.0000041
AAACACG5750.039.13043640
GACACGA5900.038.89830425
TGCGACG356.248336E-638.5714261
TGTTACG356.248336E-638.5714261
GGTTACG356.248336E-638.5714261
AACGGGA4450.038.4269684
TGGTCAA6100.038.36065714
Produced by FastQC (version 0.11.5)