FastQCFastQC Report
Sat 18 Jun 2016
SRR3554020_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554020_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1492125
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCTTCTGC29520.19783865292787134TruSeq Adapter, Index 21 (95% over 22bp)
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC28090.18825500544525425No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT21670.14522911954427412No Hit
GCTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCTTCTGC14960.10025969674122477TruSeq Adapter, Index 21 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATAT3750.042.60000214
TCGTGTA5050.040.54455617
CGTGTAC5250.040.28571318
TCGATAG451.9290383E-840.01
GCTACGA4250.039.70588310
TACGAAT4200.039.64285712
TCTACGG750.039.0000042
TCGAACG356.249542E-638.5714261
CTACGAA4400.038.35227611
CCCTCGT5350.038.27102714
TATGCGG2650.038.2075462
CTATGCG659.094947E-1238.076921
CGCATCG1300.038.0769221
ACACGAC5400.037.50000426
ACGTTAG601.5643309E-1037.4999961
CTATACG301.1400853E-437.4999961
CAATTCG301.1400853E-437.49999623
ACTATGC5450.037.1559648
CGAGACA5600.036.96428322
AGACACG5550.036.89189524
Produced by FastQC (version 0.11.5)