##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554020_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1492125
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.092706710228704	33.0	31.0	34.0	30.0	34.0
2	32.223764765016334	34.0	31.0	34.0	30.0	34.0
3	32.22686571165284	34.0	31.0	34.0	30.0	34.0
4	35.79615246711904	37.0	35.0	37.0	35.0	37.0
5	35.67586629806484	37.0	35.0	37.0	33.0	37.0
6	35.659651168635335	37.0	35.0	37.0	33.0	37.0
7	35.866719611292616	37.0	35.0	37.0	35.0	37.0
8	35.72405864119963	37.0	35.0	37.0	35.0	37.0
9	37.40096372622937	39.0	37.0	39.0	35.0	39.0
10	37.031573762251824	39.0	37.0	39.0	33.0	39.0
11	37.00714819468878	39.0	37.0	39.0	33.0	39.0
12	36.91462645555835	39.0	37.0	39.0	33.0	39.0
13	36.9035871659546	39.0	37.0	39.0	33.0	39.0
14	38.05309139649828	40.0	37.0	41.0	33.0	41.0
15	38.16694512859177	40.0	37.0	41.0	33.0	41.0
16	38.20882968920164	40.0	37.0	41.0	33.0	41.0
17	38.160505319594535	40.0	37.0	41.0	33.0	41.0
18	38.10705671441736	40.0	37.0	41.0	33.0	41.0
19	38.09032889335679	40.0	37.0	41.0	34.0	41.0
20	38.03355348915138	40.0	37.0	41.0	33.0	41.0
21	37.8994286671693	40.0	36.0	41.0	33.0	41.0
22	37.90179978218983	40.0	36.0	41.0	33.0	41.0
23	37.821382927033596	40.0	36.0	41.0	33.0	41.0
24	37.79098600988523	40.0	36.0	41.0	33.0	41.0
25	37.687982575186396	40.0	36.0	41.0	33.0	41.0
26	37.63271709809835	40.0	36.0	41.0	33.0	41.0
27	37.580189997486805	40.0	36.0	41.0	33.0	41.0
28	37.47625098433442	40.0	36.0	41.0	33.0	41.0
29	37.453947222920334	40.0	36.0	41.0	33.0	41.0
30	37.322730334254835	39.0	36.0	41.0	32.0	41.0
31	37.24323699421965	39.0	35.0	41.0	32.0	41.0
32	37.14693474072212	39.0	35.0	41.0	31.0	41.0
33	37.04448286839239	39.0	35.0	41.0	31.0	41.0
34	36.96579978218983	39.0	35.0	41.0	31.0	41.0
35	36.83797470051102	39.0	35.0	41.0	31.0	41.0
36	36.66066247800955	39.0	35.0	41.0	30.0	41.0
37	36.60059043310714	39.0	35.0	41.0	30.0	41.0
38	36.47291480271425	39.0	35.0	41.0	30.0	41.0
39	36.45470788305269	39.0	35.0	41.0	30.0	41.0
40	36.297308201390635	39.0	35.0	40.0	30.0	41.0
41	36.14318907598224	38.0	35.0	40.0	30.0	41.0
42	36.032613889587	38.0	35.0	40.0	29.0	41.0
43	35.95983848538159	38.0	35.0	40.0	29.0	41.0
44	35.88702488062327	38.0	35.0	40.0	29.0	41.0
45	35.800249979056716	38.0	35.0	40.0	29.0	41.0
46	35.62669347407221	38.0	34.0	40.0	28.0	41.0
47	35.48846912959705	38.0	34.0	40.0	28.0	41.0
48	35.349711987936665	37.0	34.0	40.0	28.0	41.0
49	35.266671357962636	37.0	34.0	40.0	27.0	41.0
50	35.16723732931222	37.0	34.0	40.0	27.0	41.0
51	33.547793918069864	35.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	8.0
10	6.0
11	10.0
12	11.0
13	23.0
14	38.0
15	73.0
16	148.0
17	251.0
18	511.0
19	843.0
20	1444.0
21	2265.0
22	3454.0
23	4798.0
24	6545.0
25	8565.0
26	11266.0
27	14138.0
28	17581.0
29	21905.0
30	27573.0
31	35003.0
32	44933.0
33	60851.0
34	100381.0
35	124257.0
36	130633.0
37	187050.0
38	294424.0
39	392992.0
40	139.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.98926028315322	18.27648487894781	23.09484795174667	17.6394068861523
2	28.445874172740222	26.246795677305855	26.761698919326466	18.54563123062746
3	31.87125743486638	24.90548714082265	26.65842338946134	16.564832034849626
4	29.56783111334506	26.298064840412167	24.654167713830947	19.479936332411828
5	24.936784786797354	31.01482784619251	23.995241685515623	20.053145681494513
6	24.495266817458322	36.39105302839909	23.61035436039206	15.503325793750525
7	81.94279969841669	6.053916394403954	7.773946552735193	4.229337354444166
8	83.89397671106643	4.8663483287258105	6.444768367261455	4.794906592946301
9	77.43379408561614	7.616721119209181	8.813202647231298	6.136282147943369
10	39.886671693055206	25.911569070955853	16.99723548630309	17.20452374968585
11	30.303225265979727	26.39832453715339	22.321856412834045	20.97659378403284
12	26.282114434112426	22.753522660635	29.105872497277375	21.858490407975204
13	24.590232051604254	24.056161514618413	30.291295970511854	21.062310463265476
14	22.320650079584485	26.83716176593784	28.478445170478345	22.36374298399933
15	20.835385775320432	25.173561196280474	31.217826924687948	22.77322610371115
16	23.382357376225183	24.117617491832117	29.74710563793248	22.75291949401022
17	23.10074558096674	24.75295300326715	26.916645723381084	25.229655692385023
18	24.313244533802465	24.26432101868141	27.90503476585407	23.51739968166206
19	25.088581720700343	26.86946468962051	25.097026053447262	22.944927536231884
20	25.810840244617577	25.37689536734523	25.677138309457987	23.13512607857921
21	25.355516461422468	26.790449861774313	25.911233978386527	21.94279969841669
22	24.098584233894613	24.61268325374885	25.416771383094577	25.871961129261962
23	22.972539163944038	26.799028231548967	25.850314149283736	24.378118455223255
24	23.239473904666163	25.303912205746837	27.575705788724136	23.880908100862865
25	24.084644383010804	26.193381921755886	25.7344391388121	23.98753455642121
26	22.531155231632738	27.02367429002262	25.4676384351177	24.97753204322694
27	23.996179944709723	25.24634330233727	26.821814526262884	23.935662226690123
28	22.286001507916563	26.171332830694478	27.491597553824242	24.051068107564717
29	24.22725978051437	25.257937505235823	26.205110161682164	24.309692552567647
30	24.759654854653597	24.279534221328642	27.207841166122144	23.75296975789562
31	25.688933567898133	26.11309374214627	24.376845103459832	23.82112758649577
32	25.224093155734273	25.663868643712828	25.26879450448186	23.84324369607104
33	24.37925776995895	25.519376727821058	25.33480774063835	24.766557761581637
34	24.747591522157997	25.464287509424476	25.88429253581302	23.903828432604506
35	25.357594035352264	24.158297729747844	26.75928625282734	23.724821982072548
36	25.185825584317666	26.72745245874173	25.51904163525174	22.567680321688865
37	23.770729664069698	26.884476836726144	26.841182876769707	22.503610622434447
38	23.262126162352352	27.07996984166876	26.562251822065846	23.09565217391304
39	25.226438803719525	24.8394068861523	25.95593532713412	23.97821898299405
40	25.637530367764093	24.16426237748178	28.25674792661473	21.9414593281394
41	22.561045488816287	25.57909022367429	27.59748680573008	24.26237748177934
42	23.349250230376143	25.8181452626288	27.34475998994722	23.487844517047833
43	23.441668760995224	23.847733936499957	28.191002764513694	24.51959453799112
44	23.958247465862446	24.061724051269163	26.811091564044563	25.168936918823825
45	24.79081846360057	23.346703526849293	26.67698751780179	25.185490491748347
46	25.26530954176091	24.369272011393146	26.19063416268744	24.1747842841585
47	23.719393482449526	24.983295635419285	28.215397503560357	23.08191337857083
48	22.746351679651504	24.49359135461171	28.270285666415347	24.48977129932144
49	23.31788556588758	24.711200469129597	28.211108318673034	23.759805646309793
50	22.580279802295383	23.625634581553154	29.620440646728657	24.173644969422803
51	22.82255172991539	23.80283153221077	27.214341961966994	26.160274775906846
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1848.0
1	2082.0
2	2316.0
3	2809.5
4	3303.0
5	2595.0
6	1887.0
7	1996.5
8	2106.0
9	2192.0
10	2278.0
11	2406.0
12	2534.0
13	2549.5
14	2565.0
15	2593.5
16	2622.0
17	2640.5
18	2659.0
19	3003.5
20	3348.0
21	3485.0
22	3622.0
23	3923.0
24	4224.0
25	6512.5
26	9833.0
27	10865.0
28	13550.5
29	16236.0
30	18619.5
31	21003.0
32	23995.5
33	26988.0
34	30811.5
35	34635.0
36	37129.0
37	39623.0
38	43711.5
39	47800.0
40	51576.5
41	55353.0
42	63893.0
43	72433.0
44	81638.0
45	90843.0
46	103216.5
47	115590.0
48	125240.0
49	134890.0
50	134088.5
51	133287.0
52	120723.5
53	108160.0
54	99253.0
55	90346.0
56	84311.5
57	78277.0
58	75269.0
59	72261.0
60	71009.0
61	69757.0
62	64540.0
63	59323.0
64	52963.0
65	46603.0
66	41081.0
67	35559.0
68	32040.5
69	28522.0
70	24758.0
71	20994.0
72	17484.5
73	13975.0
74	12025.5
75	8458.0
76	6840.0
77	5382.0
78	3924.0
79	2876.0
80	1828.0
81	1396.0
82	964.0
83	724.5
84	485.0
85	386.0
86	287.0
87	220.0
88	153.0
89	116.5
90	80.0
91	53.0
92	26.0
93	19.5
94	13.0
95	12.0
96	11.0
97	5.5
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1492125.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.188931080200188
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.04533124880443	18.399338145565277
2	10.049113061118305	5.062528326272971
3	3.711357165162958	2.804553595418907
4	1.907859851097981	1.9222780119995213
5	1.1948255403301964	1.5048189094120135
6	0.8307849747977766	1.2555951283588227
7	0.6401294155628149	1.12869230117144
8	0.5017346374447496	1.011052736251602
9	0.4329416672793612	0.981480403696211
>10	6.3021771482669395	39.230056523771594
>50	1.2929241182199331	20.815133497062956
>100	0.08227329693974464	3.5876334358520787
>500	0.005609542952211614	1.057652097721891
>1k	0.002938332022587036	1.2391868874447527
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCTTCTGC	2952	0.19783865292787134	TruSeq Adapter, Index 21 (95% over 22bp)
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	2809	0.18825500544525425	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	2167	0.14522911954427412	No Hit
GCTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCTTCTGC	1496	0.10025969674122477	TruSeq Adapter, Index 21 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.701851386445506E-5	0.0	0.0	0.08484543855240011	0.0
2	6.701851386445506E-5	0.0	0.0	0.5048504649409399	0.0
3	6.701851386445506E-5	0.0	0.0	0.7937002596967412	0.0
4	6.701851386445506E-5	0.0	0.0	1.0889168132696658	0.0
5	6.701851386445506E-5	0.0	0.0	2.048621931808662	0.0
6	6.701851386445506E-5	0.0	0.0	3.0660970092988187	0.0
7	6.701851386445506E-5	0.0	0.0	3.8331909189913715	0.0
8	6.701851386445506E-5	0.0	0.0	5.060769037446595	0.0
9	6.701851386445506E-5	0.0	0.0	5.5624696322359055	0.0
10	6.701851386445506E-5	0.0	0.0	6.491078160341795	0.0
11	6.701851386445506E-5	0.0	0.0	7.646477339364999	0.0
12	6.701851386445506E-5	0.0	0.0	8.567780849459663	0.0
13	6.701851386445506E-5	0.0	0.0	9.04086453882885	0.0
14	6.701851386445506E-5	0.0	0.0	9.183546954846276	0.0
15	6.701851386445506E-5	0.0	0.0	9.483387785875848	0.0
16	6.701851386445506E-5	0.0	0.0	9.993599731925945	0.0
17	6.701851386445506E-5	0.0	0.0	10.57344391388121	0.0
18	6.701851386445506E-5	0.0	0.0	11.2656111250733	0.0
19	6.701851386445506E-5	0.0	0.0	11.623355952081763	0.0
20	1.3403702772891011E-4	0.0	0.0	11.985322945463684	0.0
21	1.3403702772891011E-4	0.0	0.0	12.423087878026305	0.0
22	1.3403702772891011E-4	0.0	0.0	12.910044399765436	0.0
23	1.3403702772891011E-4	0.0	0.0	13.412214124151797	0.0
24	1.3403702772891011E-4	0.0	0.0	13.80534472648069	0.0
25	3.350925693222753E-4	0.0	0.0	14.171600904749937	0.0
26	3.350925693222753E-4	0.0	0.0	14.498115104297563	0.0
27	3.350925693222753E-4	0.0	0.0	14.837697914048755	0.0
28	3.350925693222753E-4	0.0	0.0	15.18270922342297	0.0
29	3.350925693222753E-4	0.0	0.0	15.523900477506912	0.0
30	4.021110831867303E-4	0.0	0.0	15.924537153388624	0.0
31	4.021110831867303E-4	0.0	0.0	16.2798022953841	0.0
32	4.021110831867303E-4	0.0	0.0	16.633123900477507	0.0
33	4.691295970511854E-4	0.0	0.0	16.99227611627712	0.0
34	5.361481109156405E-4	0.0	0.0	17.32247633408729	0.0
35	5.361481109156405E-4	0.0	0.0	17.700058641199632	0.0
36	5.361481109156405E-4	0.0	0.0	18.042054117449947	0.0
37	5.361481109156405E-4	0.0	0.0	18.379492334757476	0.0
38	6.031666247800955E-4	0.0	0.0	18.707212867554663	0.0
39	6.031666247800955E-4	0.0	0.0	19.049342380832705	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAATAT	375	0.0	42.600002	14
TCGTGTA	505	0.0	40.544556	17
CGTGTAC	525	0.0	40.285713	18
TCGATAG	45	1.9290383E-8	40.0	1
GCTACGA	425	0.0	39.705883	10
TACGAAT	420	0.0	39.642857	12
TCTACGG	75	0.0	39.000004	2
TCGAACG	35	6.249542E-6	38.571426	1
CTACGAA	440	0.0	38.352276	11
CCCTCGT	535	0.0	38.271027	14
TATGCGG	265	0.0	38.207546	2
CTATGCG	65	9.094947E-12	38.07692	1
CGCATCG	130	0.0	38.07692	21
ACACGAC	540	0.0	37.500004	26
ACGTTAG	60	1.5643309E-10	37.499996	1
CTATACG	30	1.1400853E-4	37.499996	1
CAATTCG	30	1.1400853E-4	37.499996	23
ACTATGC	545	0.0	37.155964	8
CGAGACA	560	0.0	36.964283	22
AGACACG	555	0.0	36.891895	24
>>END_MODULE