FastQCFastQC Report
Sat 18 Jun 2016
SRR3554019_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554019_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1286122
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48950.3806015292483917No Hit
GCTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCCGTCTTCTGC15300.11896227574055962No Hit
GAATCTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCCGTCTTC15180.11802923828377092No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13980.10869886371588389No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTCG453.8562575E-1045.00000440
GTACCGA207.0335995E-445.08
CTAACCG207.0335995E-445.01
AACGCGT700.045.027
CTAACGC1100.042.95454425
CGTTTTT40450.041.273181
TAGAACG403.4586446E-739.3751
CCGTCGA403.4586446E-739.37541
TCGACAG403.4586446E-739.3751
TATACGG1100.038.8636362
TGTGCGG4100.038.4146352
CGAATAT900.037.50000414
TAACGCG850.037.05882626
GCGATGC2600.036.3461579
CACGTTA751.8189894E-1236.026
CCATGCG504.879803E-835.9999961
CGTAGTA250.00210720235.99999624
CGCTAGC250.00210720235.99999621
ACGGGCC1450.035.6896555
TTAACGG950.035.5263182
Produced by FastQC (version 0.11.5)