##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554019_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1286122
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.743308955138005	31.0	31.0	34.0	30.0	34.0
2	31.875988436555787	33.0	31.0	34.0	30.0	34.0
3	31.859829782866633	33.0	31.0	34.0	30.0	34.0
4	35.515867079483904	37.0	35.0	37.0	33.0	37.0
5	35.369116615686536	37.0	35.0	37.0	33.0	37.0
6	35.326120694615284	37.0	35.0	37.0	32.0	37.0
7	35.73216304518545	37.0	35.0	37.0	35.0	37.0
8	35.64649232343432	37.0	35.0	37.0	35.0	37.0
9	37.37458499271453	39.0	37.0	39.0	34.0	39.0
10	36.65525043502871	39.0	37.0	39.0	32.0	39.0
11	36.59104968268951	39.0	35.0	39.0	32.0	39.0
12	36.63259317545303	39.0	35.0	39.0	32.0	39.0
13	36.602981676699414	39.0	35.0	39.0	32.0	39.0
14	37.69478245454164	39.0	37.0	41.0	33.0	41.0
15	37.80673839651293	40.0	37.0	41.0	33.0	41.0
16	37.821754079317515	40.0	37.0	41.0	33.0	41.0
17	37.760079525892564	40.0	37.0	41.0	33.0	41.0
18	37.703865574183475	39.0	36.0	41.0	33.0	41.0
19	37.62348439728113	39.0	36.0	41.0	32.0	41.0
20	37.5253311894206	39.0	36.0	41.0	32.0	41.0
21	37.44853365388354	39.0	36.0	41.0	32.0	41.0
22	37.44560236120679	39.0	36.0	41.0	32.0	41.0
23	36.94343149405733	39.0	35.0	41.0	31.0	41.0
24	37.04732754746439	39.0	35.0	41.0	31.0	41.0
25	37.06765143586689	39.0	35.0	41.0	31.0	41.0
26	37.096900605074794	39.0	35.0	41.0	31.0	41.0
27	34.679508631374006	39.0	35.0	40.0	24.0	41.0
28	35.5793338423571	39.0	35.0	40.0	23.0	41.0
29	36.25487784207097	39.0	35.0	40.0	29.0	41.0
30	36.52766300553136	39.0	35.0	40.0	30.0	41.0
31	36.59287143832389	39.0	35.0	40.0	30.0	41.0
32	36.33489824449003	39.0	35.0	40.0	30.0	41.0
33	36.322001334243566	39.0	35.0	40.0	30.0	41.0
34	36.2790046356411	39.0	35.0	40.0	30.0	41.0
35	36.16527903262676	39.0	35.0	40.0	29.0	41.0
36	36.07605654829013	39.0	35.0	40.0	29.0	41.0
37	36.01676823816092	39.0	35.0	40.0	29.0	41.0
38	35.948144110745325	39.0	35.0	40.0	29.0	41.0
39	35.84635205680332	38.0	35.0	40.0	28.0	41.0
40	35.760630795523284	38.0	35.0	40.0	28.0	41.0
41	35.6565613526555	38.0	35.0	40.0	27.0	41.0
42	35.5548696002401	38.0	34.0	40.0	27.0	41.0
43	35.444668546218786	38.0	34.0	40.0	26.0	41.0
44	35.33883332996403	38.0	34.0	40.0	26.0	41.0
45	35.295998357854074	38.0	34.0	40.0	26.0	41.0
46	35.15234246828839	38.0	34.0	40.0	26.0	41.0
47	35.04244620650296	38.0	34.0	40.0	26.0	41.0
48	34.94531778478247	38.0	34.0	40.0	25.0	41.0
49	34.8885603387548	38.0	34.0	40.0	26.0	41.0
50	34.78095701651943	37.0	34.0	40.0	25.0	41.0
51	33.22828394195885	36.0	31.0	39.0	21.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	0.0
10	4.0
11	4.0
12	16.0
13	17.0
14	28.0
15	77.0
16	166.0
17	355.0
18	671.0
19	1246.0
20	2026.0
21	3157.0
22	4659.0
23	6558.0
24	8871.0
25	12447.0
26	15236.0
27	18107.0
28	20524.0
29	24112.0
30	29818.0
31	37041.0
32	46573.0
33	60478.0
34	86042.0
35	105205.0
36	121147.0
37	162616.0
38	235674.0
39	283183.0
40	61.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.85745986772639	17.433649373854113	23.129920800670543	17.578969957748956
2	32.04392740346561	24.498297984172574	25.713423765397064	17.74435084696475
3	32.04828157826396	24.591290717365847	27.15473337677141	16.20569432759878
4	27.37228661044598	26.688992179591047	27.12168830017681	18.817032909786164
5	24.395974876411415	30.955850222607182	25.90181957854698	18.746355322434418
6	24.313323308364215	35.988887523889645	25.54329993577592	14.154489231970219
7	82.36924646339926	5.264430590566058	8.622199138184403	3.7441238078502663
8	84.28422809033668	4.498018072935539	7.571132443111929	3.6466213936158467
9	77.60492394967196	7.539953441430907	9.950766723530116	4.904355885367018
10	34.93315564153323	32.52941789348133	17.859658725999555	14.677767738985882
11	26.339725158266482	27.036859644730438	28.816162074826497	17.80725312217659
12	23.668905438208817	23.85434663274557	32.60071750580427	19.876030423241343
13	23.450185907713266	24.349634016057575	33.63117962370599	18.569000452523166
14	21.587065612749022	27.125187190639764	31.094484038061708	20.1932631585495
15	20.40630671118292	27.119666719020437	33.32685390655008	19.147172663246568
16	22.997818247413544	26.294317335369428	30.81270672611152	19.89515769110551
17	23.457572454246176	25.77142759396076	30.085248522301928	20.685751429491138
18	23.34996213422988	24.96193984707516	30.888049500747204	20.800048517947754
19	22.596067869144605	27.844325810459665	29.476985853597093	20.08262046679864
20	23.69262014023553	26.859504774819186	29.494869071518874	19.953006013426407
21	23.646512539245887	27.740758652756114	29.583196617428207	19.02953219056979
22	22.51901452583814	25.410964123154724	29.19676360407489	22.873257746932254
23	21.41787482058467	28.366049255047344	28.980609926585505	21.235465997782484
24	21.08198133614074	26.99386216859676	30.60207352024147	21.322082975021033
25	21.416941783127882	28.29055097416886	28.881085931194704	21.411421311508548
26	21.43459174168547	27.963288086200222	29.33990710057055	21.262213071543755
27	20.446738334310428	31.78811963406271	27.523516431567145	20.241625600059717
28	21.238576122638445	27.291423364190955	30.583723783591292	20.886276729579308
29	21.9937921907875	26.64863830958494	29.529857976148456	21.82771152347911
30	23.38370698891707	26.215164657785188	29.920489658057324	20.480638695240422
31	23.388061163715417	26.419810873307508	28.66267741318475	21.529450549792323
32	23.111648817141763	27.310395125812327	28.83777744257543	20.740178614470477
33	22.596067869144605	26.75158344231729	28.726046207124984	21.926302481413114
34	22.678874943434604	26.453322468630503	29.432433315035432	21.43536927289946
35	22.974492310993824	26.406359583305473	28.918951701316047	21.700196404384652
36	23.316295032664087	26.751272429831698	29.337107988200184	20.59532454930403
37	22.77334498593446	27.421271076927383	29.282680803220845	20.52270313391731
38	21.646702256862103	27.902562898387558	29.0966953368343	21.354039507916045
39	23.273219803409006	26.463119361926786	28.488899186857857	21.77476164780635
40	23.687021915494796	25.89855394744822	29.475119778683517	20.939304358373466
41	22.659592169327638	25.76388554118505	28.559810033573797	23.016712255913514
42	23.0837354465595	26.63122161039155	28.192037769356247	22.0930051736927
43	22.469796799992537	26.05950290874427	29.49867897446743	21.97202131679576
44	22.399663484490585	26.81798460799209	28.93629064738804	21.846061260129286
45	22.380769475990615	26.182041828069185	28.90487838634282	22.532310309597378
46	23.19733275692353	26.717838587630098	28.431672889508146	21.653155765938223
47	22.469796799992537	26.228615947787226	29.36587664311784	21.9357106091024
48	22.20201504989418	25.935486680112774	29.32101309207058	22.541485177922468
49	22.768213279922122	25.34915039164247	29.254611926395786	22.62802440203962
50	21.62951881703291	25.427836550498316	30.288650687881862	22.653993944586905
51	21.727876515602716	25.083156963336293	28.803721575402648	24.385244945658343
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1648.0
1	1971.0
2	2294.0
3	5896.0
4	9498.0
5	6872.5
6	4247.0
7	4256.0
8	4265.0
9	4506.5
10	4748.0
11	4816.0
12	4884.0
13	4809.5
14	4735.0
15	4682.5
16	4630.0
17	4534.0
18	4438.0
19	4721.0
20	5004.0
21	5197.0
22	5390.0
23	6198.0
24	7006.0
25	8291.5
26	11652.0
27	13727.0
28	16287.0
29	18847.0
30	22111.0
31	25375.0
32	28631.0
33	31887.0
34	35759.5
35	39632.0
36	44251.0
37	48870.0
38	52503.0
39	56136.0
40	61791.0
41	67446.0
42	70561.5
43	73677.0
44	81219.5
45	88762.0
46	99277.5
47	109793.0
48	112680.5
49	115568.0
50	113668.5
51	111769.0
52	100583.0
53	89397.0
54	78370.0
55	67343.0
56	61898.0
57	56453.0
58	50847.0
59	45241.0
60	42439.0
61	39637.0
62	35291.5
63	30946.0
64	26756.5
65	22567.0
66	19567.0
67	16567.0
68	14722.5
69	12878.0
70	11455.0
71	10032.0
72	8799.5
73	7567.0
74	6465.5
75	4382.5
76	3401.0
77	2765.0
78	2129.0
79	1663.5
80	1198.0
81	1004.0
82	810.0
83	568.0
84	326.0
85	291.5
86	257.0
87	166.0
88	75.0
89	55.0
90	35.0
91	30.0
92	25.0
93	15.5
94	6.0
95	8.5
96	11.0
97	7.0
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1286122.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.99600141458867
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.59455081059885	24.613219040671257
2	8.926070617067348	5.890492774149451
3	3.514211957313614	3.478648281440533
4	1.9909372499534759	2.6277187326328866
5	1.3692542418895393	2.258995745115939
6	1.0081132125021675	1.9958222991472228
7	0.8473689170714431	1.9571850190457465
8	0.6959999516764863	1.837217231205679
9	0.5746765702173401	1.706582603144009
>10	6.07649051186439	42.712486924904965
>50	0.3656458256300816	7.472840141400041
>100	0.03257775085200049	1.9123500759187513
>500	0.0028958000211279163	0.707546428899887
>1k	0.001206583342136632	0.8288947023236354
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4895	0.3806015292483917	No Hit
GCTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCCGTCTTCTGC	1530	0.11896227574055962	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCCGTCTTC	1518	0.11802923828377092	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1398	0.10869886371588389	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.775312139905857E-5	0.0	0.0	0.09151542388669193	0.0
2	7.775312139905857E-5	0.0	0.0	0.38915437260228813	0.0
3	7.775312139905857E-5	0.0	0.0	0.6049192844846757	0.0
4	7.775312139905857E-5	0.0	0.0	0.8861523245850704	0.0
5	7.775312139905857E-5	0.0	0.0	1.6142325533658548	0.0
6	7.775312139905857E-5	0.0	0.0	2.40832518221444	0.0
7	7.775312139905857E-5	0.0	0.0	3.049555174392476	0.0
8	7.775312139905857E-5	0.0	0.0	4.185994796761116	0.0
9	7.775312139905857E-5	0.0	0.0	4.74721682701952	0.0
10	1.5550624279811714E-4	0.0	0.0	5.861496809789429	0.0
11	1.5550624279811714E-4	0.0	0.0	6.895224558789913	0.0
12	1.5550624279811714E-4	0.0	0.0	7.825773915693846	0.0
13	1.5550624279811714E-4	0.0	0.0	8.248828649226123	0.0
14	1.5550624279811714E-4	0.0	0.0	8.448576418100304	0.0
15	1.5550624279811714E-4	0.0	0.0	8.700418778311855	0.0
16	1.5550624279811714E-4	0.0	0.0	9.169036840983981	0.0
17	1.5550624279811714E-4	0.0	0.0	9.707865972279457	0.0
18	1.5550624279811714E-4	0.0	0.0	10.356560264111803	0.0
19	1.5550624279811714E-4	0.0	0.0	10.701706370002224	0.0
20	1.5550624279811714E-4	0.0	0.0	11.047474500863837	0.0
21	1.5550624279811714E-4	0.0	0.0	11.41478024635299	0.0
22	1.5550624279811714E-4	0.0	0.0	11.825472233582817	0.0
23	1.5550624279811714E-4	0.0	0.0	12.234686911506062	0.0
24	1.5550624279811714E-4	0.0	0.0	12.573612767684558	0.0
25	1.5550624279811714E-4	0.0	0.0	12.86588675102362	0.0
26	1.5550624279811714E-4	0.0	0.0	13.148052828580804	0.0
27	2.3325936419717568E-4	0.0	0.0	13.464197020189376	0.0
28	2.3325936419717568E-4	0.0	0.0	13.780263458676549	0.0
29	2.3325936419717568E-4	0.0	0.0	14.118489536762453	0.0
30	2.3325936419717568E-4	0.0	0.0	14.543565851451106	0.0
31	2.3325936419717568E-4	0.0	0.0	14.92066849023654	0.0
32	2.3325936419717568E-4	0.0	0.0	15.289529298153674	0.0
33	2.3325936419717568E-4	0.0	0.0	15.637707775778658	0.0
34	2.3325936419717568E-4	0.0	0.0	15.98697479710323	0.0
35	2.3325936419717568E-4	0.0	0.0	16.363299904674673	0.0
36	2.3325936419717568E-4	0.0	0.0	16.717698632011583	0.0
37	2.3325936419717568E-4	0.0	0.0	17.090058330391674	0.0
38	2.3325936419717568E-4	0.0	0.0	17.445079082699774	0.0
39	2.3325936419717568E-4	0.0	0.0	17.813162359402916	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCGTCG	45	3.8562575E-10	45.000004	40
GTACCGA	20	7.0335995E-4	45.0	8
CTAACCG	20	7.0335995E-4	45.0	1
AACGCGT	70	0.0	45.0	27
CTAACGC	110	0.0	42.954544	25
CGTTTTT	4045	0.0	41.27318	1
TAGAACG	40	3.4586446E-7	39.375	1
CCGTCGA	40	3.4586446E-7	39.375	41
TCGACAG	40	3.4586446E-7	39.375	1
TATACGG	110	0.0	38.863636	2
TGTGCGG	410	0.0	38.414635	2
CGAATAT	90	0.0	37.500004	14
TAACGCG	85	0.0	37.058826	26
GCGATGC	260	0.0	36.346157	9
CACGTTA	75	1.8189894E-12	36.0	26
CCATGCG	50	4.879803E-8	35.999996	1
CGTAGTA	25	0.002107202	35.999996	24
CGCTAGC	25	0.002107202	35.999996	21
ACGGGCC	145	0.0	35.689655	5
TTAACGG	95	0.0	35.526318	2
>>END_MODULE