FastQCFastQC Report
Sat 18 Jun 2016
SRR3554017_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554017_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences360086
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12010.3335314341573957No Hit
GCTGTCTCTTATACACATCTGACGCAAGCCCATTCGTATGCCGTCTTCTGC7090.1968974078414601No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6290.17468049299334049No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5340.14829790661119843No Hit
CCTGTCTCTTATACACATCTGACGCAAGCCCATTCGTATGCCGTCTTCTGC5110.14191054359236405No Hit
CTGTCTCTTATACACATCTGACGCAAGCCCATTCGTATGCCGTCTTCTGCT5090.14135512072116105No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA4830.13413462339552218No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA4640.12885810611909376No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC4100.11386168859661304No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAGCCCATTCGTATGCC3620.10053153968774126No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCGTG302.1613341E-645.0000041
TAGGTCG302.1613341E-645.0000041
TCGGGTA302.1613341E-645.00000427
ACCTAAT302.1613341E-645.00000422
TCGTTTA207.0268277E-445.038
ACTATAT207.0268277E-445.037
TCGTTGC207.0268277E-445.016
TCGTTGA207.0268277E-445.024
GGTAAGT207.0268277E-445.08
GTCGAAT207.0268277E-445.028
ACGCATT406.7921064E-945.017
CGCATCG207.0268277E-445.021
GAGTACG253.8854523E-545.01
TAAACGG253.8854523E-545.02
GCACGAG253.8854523E-545.01
CGATTAG207.0268277E-445.010
AATATCA207.0268277E-445.026
CTAACGG207.0268277E-445.01
CGCTCGT207.0268277E-445.013
CGCTCGA207.0268277E-445.020