##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554017_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	360086
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.78728970301539	31.0	31.0	34.0	30.0	34.0
2	31.889862421754803	33.0	31.0	34.0	30.0	34.0
3	31.930000055542287	33.0	31.0	34.0	30.0	34.0
4	35.564745644096135	37.0	35.0	37.0	33.0	37.0
5	35.39894080858462	37.0	35.0	37.0	33.0	37.0
6	35.342820881678264	37.0	35.0	37.0	33.0	37.0
7	35.62401759579656	37.0	35.0	37.0	35.0	37.0
8	35.473100870347636	37.0	35.0	37.0	33.0	37.0
9	37.238220869458964	39.0	37.0	39.0	34.0	39.0
10	36.71087462439528	39.0	37.0	39.0	32.0	39.0
11	36.612495348333454	39.0	35.0	39.0	32.0	39.0
12	36.48344006709508	38.0	35.0	39.0	32.0	39.0
13	36.3120199063557	38.0	35.0	39.0	32.0	39.0
14	37.36969779441578	39.0	36.0	41.0	32.0	41.0
15	37.52110606910571	39.0	36.0	41.0	32.0	41.0
16	37.563754214271036	39.0	36.0	41.0	33.0	41.0
17	37.502063395966516	39.0	36.0	41.0	32.0	41.0
18	37.46227845570225	39.0	36.0	41.0	32.0	41.0
19	37.38806562876646	39.0	36.0	41.0	32.0	41.0
20	37.29593208289131	39.0	35.0	41.0	32.0	41.0
21	37.149939181195606	39.0	35.0	41.0	32.0	41.0
22	37.12538393605972	39.0	35.0	41.0	32.0	41.0
23	36.673344700988096	39.0	35.0	41.0	31.0	41.0
24	36.74840177068811	39.0	35.0	41.0	31.0	41.0
25	36.732141766133644	39.0	35.0	40.0	31.0	41.0
26	36.76129313552873	39.0	35.0	40.0	31.0	41.0
27	34.421379892581214	38.0	34.0	40.0	23.0	41.0
28	35.222044178335175	38.0	34.0	40.0	22.0	41.0
29	35.84914992529562	38.0	34.0	40.0	27.0	41.0
30	36.13962220136301	38.0	35.0	40.0	30.0	41.0
31	36.172489349766444	38.0	35.0	40.0	30.0	41.0
32	35.973028665374386	38.0	35.0	40.0	29.0	41.0
33	35.975786339929904	38.0	35.0	40.0	29.0	41.0
34	35.90681948201263	38.0	35.0	40.0	29.0	41.0
35	35.794471320740044	38.0	35.0	40.0	29.0	41.0
36	35.69113767266708	38.0	35.0	40.0	28.0	41.0
37	35.63844748199041	38.0	34.0	40.0	28.0	41.0
38	35.530653788261695	38.0	34.0	40.0	28.0	41.0
39	35.42374043978383	38.0	34.0	40.0	27.0	41.0
40	35.2709741561738	38.0	34.0	40.0	27.0	41.0
41	35.12582827435668	38.0	34.0	40.0	26.0	41.0
42	35.04681381669934	37.0	34.0	40.0	26.0	41.0
43	34.94351627111301	37.0	34.0	40.0	26.0	41.0
44	34.81817676888299	37.0	34.0	40.0	26.0	41.0
45	34.72116383308432	37.0	34.0	40.0	25.0	41.0
46	34.5661619724177	37.0	33.0	40.0	24.0	41.0
47	34.44298861938537	37.0	33.0	40.0	24.0	41.0
48	34.38117283093483	36.0	33.0	40.0	24.0	41.0
49	34.327713379581546	36.0	33.0	40.0	24.0	41.0
50	34.207039429469624	36.0	33.0	40.0	24.0	41.0
51	32.68355615047516	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	4.0
11	2.0
12	6.0
13	4.0
14	15.0
15	21.0
16	69.0
17	141.0
18	276.0
19	466.0
20	759.0
21	1056.0
22	1573.0
23	2081.0
24	2825.0
25	3371.0
26	4185.0
27	5093.0
28	6025.0
29	7352.0
30	9072.0
31	11192.0
32	14363.0
33	19136.0
34	29023.0
35	34804.0
36	34149.0
37	45234.0
38	61338.0
39	66439.0
40	11.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.27743927839461	18.566953450009162	23.19973561871331	14.955871652882921
2	28.375165932582775	27.343745660758817	25.45919585876707	18.821892547891338
3	32.9118599445688	25.333114867003992	25.245080341918317	16.50994484650889
4	27.825852712963012	27.876951617113686	25.08400770926945	19.213187960653844
5	24.87905666979555	31.649661469760005	23.969829429636253	19.501452430808197
6	23.7371072466022	37.335803113700614	23.810145354165392	15.116944285531789
7	80.62573940669729	7.623734330132247	7.365740406458457	4.384785856712008
8	82.28978632882145	6.431796848530628	6.559544108907317	4.718872713740606
9	76.73777930827636	8.47519759168643	9.016179468238143	5.77084363179907
10	41.90054598068239	26.243175241470095	15.711802180590192	16.14447659725732
11	32.93213287936771	26.905794726815262	21.090517265319953	19.07155512849708
12	28.73896791322073	23.33331481923763	27.137406064106905	20.790311203434737
13	25.163155468415876	25.79467127297368	28.614553190071263	20.42762006853918
14	20.674505534788914	28.908371888937644	28.038579672633762	22.378542903639687
15	21.5076398415934	25.84243763989714	31.583566148086845	21.06635637042262
16	23.312764173003117	24.943208011419493	29.040007109412752	22.704020706164638
17	22.436584593680397	24.731036474619952	27.08380775703582	25.74857117466383
18	24.78435707025544	25.416150586248843	27.257932827157955	22.541559516337763
19	25.520570086035004	27.799192415145267	24.808517965152767	21.87171953366696
20	25.97323972606544	27.24543581255589	24.510533594752363	22.270790866626307
21	25.75412540337586	28.56984164893942	24.72298284298751	20.95305010469721
22	23.065600995317787	25.947967985425702	24.971534577850846	26.014896441405668
23	22.818437817632454	28.393772598768074	24.6846586648745	24.103130918724972
24	22.60015662924968	26.24956260448893	27.09102825436146	24.059252511899935
25	23.14502646589981	27.74087301366896	25.314230489383093	23.79987003104814
26	21.662880534094633	28.98085457362963	24.864060252273067	24.492204640002665
27	22.280233055436756	31.97680554089856	23.81875440866904	21.92420699499564
28	22.784834733924676	27.86251062246241	26.57476269557828	22.777891948034636
29	22.876201796237567	28.519298167659947	24.324744644334963	24.279755391767523
30	24.28475419760835	27.329026954671942	24.34029648472865	24.045922362991064
31	25.49974172836489	28.81395000083313	21.695095060624407	23.99121321017757
32	25.43670123248335	28.827835572613207	22.573218619996336	23.162244574907106
33	23.86346594980088	27.702826546991556	22.343273551318298	26.09043395188927
34	22.43936170803641	28.27102414423221	24.436106930011164	24.85350721772021
35	22.74512199863366	27.69866087545753	23.245558005587554	26.310659120321255
36	23.69822764561799	29.456851974250597	23.099204079025565	23.745716301105844
37	24.137567136739555	27.893336591814176	24.27447887449109	23.694617396955174
38	23.453286159417473	28.54401448542848	23.647406452902917	24.35529290225113
39	24.207550418511133	25.185372383264	24.874613286825927	25.732463911398945
40	24.553301155834994	25.62610043156357	25.632487794582403	24.188110618019028
41	20.92527896113706	26.714729259121434	26.05516459956788	26.304827180173625
42	22.803996822981176	27.07686497114578	25.322006409579934	24.797131796293108
43	23.637686552656863	24.63605916364424	26.922735124386953	24.803519159311943
44	24.057863954721928	24.763251001149726	25.91186549879751	25.26701954533084
45	23.899846147864675	24.67188393883683	25.02374432774393	26.404525585554563
46	23.804591125453364	26.45118110673561	24.6843809534389	25.059846814372122
47	22.38659653527213	25.86909793771488	26.986608754575297	24.757696772437697
48	22.277178229645138	25.873541320684502	26.53977105469249	25.309509394977862
49	23.39524446937676	25.173986214404337	27.265431035919196	24.165338280299707
50	22.355492854484762	25.178985020245165	26.93023333314819	25.53528879212188
51	22.31800181067856	25.31839616091711	25.251189993501555	27.11241203490277
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1329.0
1	1476.0
2	1623.0
3	1561.5
4	1500.0
5	1180.5
6	861.0
7	877.0
8	893.0
9	935.5
10	978.0
11	1090.0
12	1202.0
13	1196.5
14	1191.0
15	1178.5
16	1166.0
17	1205.0
18	1244.0
19	1202.0
20	1160.0
21	1187.5
22	1215.0
23	1062.0
24	909.0
25	1380.0
26	2319.0
27	2787.0
28	3164.5
29	3542.0
30	4119.0
31	4696.0
32	5400.5
33	6105.0
34	6608.0
35	7111.0
36	7819.0
37	8527.0
38	8846.5
39	9166.0
40	10830.5
41	12495.0
42	14956.0
43	17417.0
44	19383.5
45	21350.0
46	24571.5
47	27793.0
48	29978.0
49	32163.0
50	31615.5
51	31068.0
52	28459.5
53	25851.0
54	23343.0
55	20835.0
56	19945.0
57	19055.0
58	17969.0
59	16883.0
60	16518.0
61	16153.0
62	15011.5
63	13870.0
64	12406.0
65	10942.0
66	10040.0
67	9138.0
68	7886.5
69	6635.0
70	6248.0
71	5861.0
72	5221.5
73	4582.0
74	4010.5
75	2942.0
76	2445.0
77	1977.5
78	1510.0
79	1097.5
80	685.0
81	591.0
82	497.0
83	329.5
84	162.0
85	131.5
86	101.0
87	74.5
88	48.0
89	34.0
90	20.0
91	18.5
92	17.0
93	13.0
94	9.0
95	7.5
96	6.0
97	3.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	360086.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.201585500310635
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.81594637106917	26.272271575973992
2	8.431072317074914	5.767120814234797
3	3.1252941405414005	3.206700442840397
4	1.6806096381392046	2.2991805692585654
5	1.1061294199392688	1.8915689965230957
6	0.8088641141821595	1.6598661095600493
7	0.6770455928092816	1.6209222911052603
8	0.5380612387777833	1.4722037969969133
9	0.4491881401652167	1.382665192342756
>10	6.106032680983351	46.330477549046925
>50	0.22216973838738988	4.671040407558427
>100	0.03473926818421005	2.2950406166921202
>500	0.004039449788861634	0.7990919171049377
>1k	8.078899577723267E-4	0.331849720761767
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1201	0.3335314341573957	No Hit
GCTGTCTCTTATACACATCTGACGCAAGCCCATTCGTATGCCGTCTTCTGC	709	0.1968974078414601	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	629	0.17468049299334049	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	534	0.14829790661119843	No Hit
CCTGTCTCTTATACACATCTGACGCAAGCCCATTCGTATGCCGTCTTCTGC	511	0.14191054359236405	No Hit
CTGTCTCTTATACACATCTGACGCAAGCCCATTCGTATGCCGTCTTCTGCT	509	0.14135512072116105	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	483	0.13413462339552218	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	464	0.12885810611909376	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	410	0.11386168859661304	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAGCCCATTCGTATGCC	362	0.10053153968774126	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.15940636403525824	0.0
2	0.0	0.0	0.0	0.6815038629660692	0.0
3	0.0	0.0	0.0	0.9183917175341446	0.0
4	0.0	0.0	0.0	1.240536982831879	0.0
5	0.0	0.0	0.0	2.109218353393356	0.0
6	0.0	0.0	0.0	2.9159700738156995	0.0
7	0.0	0.0	0.0	3.6280221946979334	0.0
8	0.0	0.0	0.0	4.9288225590553365	0.0
9	0.0	0.0	0.0	5.586720949995279	0.0
10	0.0	0.0	0.0	6.8902984287086975	0.0
11	0.0	0.0	0.0	8.13333481446099	0.0
12	0.0	0.0	0.0	9.175863543709003	0.0
13	0.0	0.0	0.0	9.590209005626434	0.0
14	0.0	0.0	0.0	9.761556961392555	0.0
15	0.0	0.0	0.0	10.017884616452736	0.0
16	0.0	0.0	0.0	10.558588781568847	0.0
17	0.0	0.0	0.0	11.138172547669168	0.0
18	0.0	0.0	0.0	11.917430835966963	0.0
19	0.0	0.0	0.0	12.238742966957894	0.0
20	0.0	0.0	0.0	12.570330421066078	0.0
21	0.0	0.0	0.0	12.918580561310353	0.0
22	0.0	0.0	0.0	13.270440950217449	0.0
23	0.0	0.0	0.0	13.653960442783113	0.0
24	0.0	0.0	0.0	13.976105708080848	0.0
25	2.777114356014952E-4	0.0	0.0	14.24215326338708	0.0
26	2.777114356014952E-4	0.0	0.0	14.48154052087557	0.0
27	2.777114356014952E-4	0.0	0.0	14.750642901973418	0.0
28	2.777114356014952E-4	0.0	0.0	15.036685680642957	0.0
29	2.777114356014952E-4	0.0	0.0	15.337447165399377	0.0
30	2.777114356014952E-4	0.0	0.0	15.675699693961999	0.0
31	2.777114356014952E-4	0.0	0.0	15.97896058163883	0.0
32	2.777114356014952E-4	0.0	0.0	16.264170226001568	0.0
33	2.777114356014952E-4	0.0	0.0	16.557711213432345	0.0
34	5.554228712029904E-4	0.0	0.0	16.865970906950007	0.0
35	5.554228712029904E-4	0.0	0.0	17.170620351804846	0.0
36	5.554228712029904E-4	0.0	0.0	17.456940841909987	0.0
37	5.554228712029904E-4	0.0	0.0	17.755758346617196	0.0
38	5.554228712029904E-4	0.0	0.0	18.050965602661588	0.0
39	5.554228712029904E-4	0.0	0.0	18.34561743583477	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAGCGTG	30	2.1613341E-6	45.000004	1
TAGGTCG	30	2.1613341E-6	45.000004	1
TCGGGTA	30	2.1613341E-6	45.000004	27
ACCTAAT	30	2.1613341E-6	45.000004	22
TCGTTTA	20	7.0268277E-4	45.0	38
ACTATAT	20	7.0268277E-4	45.0	37
TCGTTGC	20	7.0268277E-4	45.0	16
TCGTTGA	20	7.0268277E-4	45.0	24
GGTAAGT	20	7.0268277E-4	45.0	8
GTCGAAT	20	7.0268277E-4	45.0	28
ACGCATT	40	6.7921064E-9	45.0	17
CGCATCG	20	7.0268277E-4	45.0	21
GAGTACG	25	3.8854523E-5	45.0	1
TAAACGG	25	3.8854523E-5	45.0	2
GCACGAG	25	3.8854523E-5	45.0	1
CGATTAG	20	7.0268277E-4	45.0	10
AATATCA	20	7.0268277E-4	45.0	26
CTAACGG	20	7.0268277E-4	45.0	1
CGCTCGT	20	7.0268277E-4	45.0	13
CGCTCGA	20	7.0268277E-4	45.0	20
>>END_MODULE