FastQCFastQC Report
Sat 18 Jun 2016
SRR3554014_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554014_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences635469
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTCTTCTGC10190.1603540062536489No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8790.13832303385373637No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8260.12998273715948377No Hit
CTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTCTTCTGCT7120.11204323106241217No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTCGC207.030902E-445.00000444
AAGCGTA207.030902E-445.00000422
CGTTAGA207.030902E-445.00000420
AGCGGAT207.030902E-445.0000044
CACTCCG207.030902E-445.00000435
CCATAGT207.030902E-445.00000425
CCGGTCG207.030902E-445.0000045
ATAACGC207.030902E-445.00000411
TATACGG207.030902E-445.0000042
GCGTAAG406.8066583E-945.0000041
CGCATCG253.8888298E-545.021
TACGCAT453.8380676E-1045.016
CTACGCA453.8380676E-1045.015
CGAATAT453.8380676E-1045.014
GCTACGA453.8380676E-1045.010
CCAGTCG253.8888298E-545.028
GATTACC453.8380676E-1045.09
TGATGCG351.2107739E-745.01
GCGATTC600.044.9999969
CGTAAGG950.042.6315772