##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554014_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	635469
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.854520047398065	31.0	31.0	34.0	30.0	34.0
2	31.958443291490223	33.0	31.0	34.0	30.0	34.0
3	31.982268214499843	33.0	31.0	34.0	30.0	34.0
4	35.61067809759406	37.0	35.0	37.0	33.0	37.0
5	35.464398735422186	37.0	35.0	37.0	33.0	37.0
6	35.42333615015052	37.0	35.0	37.0	33.0	37.0
7	35.821427953212506	37.0	35.0	37.0	35.0	37.0
8	35.742017313197024	37.0	35.0	37.0	35.0	37.0
9	37.49127809539096	39.0	37.0	39.0	35.0	39.0
10	36.79265707689911	39.0	37.0	39.0	32.0	39.0
11	36.70924309447038	39.0	37.0	39.0	32.0	39.0
12	36.75721711051208	39.0	35.0	39.0	32.0	39.0
13	36.71140055612469	39.0	35.0	39.0	33.0	39.0
14	37.843846041270304	40.0	37.0	41.0	33.0	41.0
15	37.957203262472284	40.0	37.0	41.0	33.0	41.0
16	37.967806454760186	40.0	37.0	41.0	33.0	41.0
17	37.90411806083381	40.0	37.0	41.0	33.0	41.0
18	37.89984562582911	40.0	37.0	41.0	33.0	41.0
19	37.856280951549174	40.0	37.0	41.0	33.0	41.0
20	37.80918345348081	40.0	36.0	41.0	33.0	41.0
21	37.71810111901603	39.0	36.0	41.0	33.0	41.0
22	37.73330721089463	39.0	36.0	41.0	33.0	41.0
23	37.163037063963785	39.0	36.0	41.0	32.0	41.0
24	37.33287697747648	39.0	36.0	41.0	32.0	41.0
25	37.3925415716581	39.0	36.0	41.0	32.0	41.0
26	37.431146129866285	39.0	36.0	41.0	32.0	41.0
27	34.802876300810894	39.0	35.0	41.0	24.0	41.0
28	35.81324974152948	39.0	35.0	41.0	23.0	41.0
29	36.61783186906049	39.0	35.0	41.0	30.0	41.0
30	36.95282696716913	39.0	35.0	41.0	31.0	41.0
31	37.068362107356926	39.0	35.0	41.0	31.0	41.0
32	36.8672334921137	39.0	35.0	41.0	31.0	41.0
33	36.90397171223144	39.0	35.0	41.0	31.0	41.0
34	36.92599481642692	39.0	35.0	41.0	31.0	41.0
35	36.862545615915174	39.0	35.0	41.0	31.0	41.0
36	36.79467763179636	39.0	35.0	41.0	31.0	41.0
37	36.76199468424109	39.0	35.0	41.0	30.0	41.0
38	36.68433865381317	39.0	35.0	41.0	30.0	41.0
39	36.626900761484826	39.0	35.0	41.0	30.0	41.0
40	36.51823771104491	39.0	35.0	40.0	30.0	41.0
41	36.43835025784106	39.0	35.0	40.0	30.0	41.0
42	36.36180993880111	39.0	35.0	40.0	30.0	41.0
43	36.246654046066766	39.0	35.0	40.0	30.0	41.0
44	36.1297167918498	38.0	35.0	40.0	30.0	41.0
45	36.10824918288697	38.0	35.0	40.0	30.0	41.0
46	35.983966173015524	38.0	35.0	40.0	29.0	41.0
47	35.89495160267456	38.0	35.0	40.0	29.0	41.0
48	35.82757931543474	38.0	35.0	40.0	29.0	41.0
49	35.76788639571718	38.0	35.0	40.0	28.0	41.0
50	35.64137038942891	38.0	34.0	40.0	28.0	41.0
51	34.10474153735273	37.0	32.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	1.0
11	6.0
12	2.0
13	10.0
14	8.0
15	25.0
16	61.0
17	122.0
18	237.0
19	405.0
20	714.0
21	1044.0
22	1572.0
23	2157.0
24	2953.0
25	4025.0
26	5199.0
27	6487.0
28	8046.0
29	9910.0
30	12949.0
31	16437.0
32	20980.0
33	28343.0
34	41281.0
35	51808.0
36	58408.0
37	80399.0
38	122527.0
39	159315.0
40	36.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.36219548081811	17.98089285236573	24.14830621163267	13.508605455183496
2	30.29746533662539	24.854713605227005	27.77287326368399	17.074947794463615
3	32.838265910689586	24.94724368930664	26.309701968152655	15.904788431851122
4	27.805604994106716	27.286775594088773	25.904489440082834	19.003129971721673
5	24.802626091910067	32.05600902640412	24.686176666367675	18.455188215318135
6	25.28652066426529	37.996188641774815	23.16572484259657	13.551565851363323
7	85.37237851098952	5.282240361056164	5.844502249519646	3.500878878434668
8	87.16632912069669	4.445535502125202	4.937140914820393	3.4509944623577233
9	80.77325565841922	7.820680473791798	7.435138456793329	3.9709254109956587
10	34.84009448139878	38.93738325551679	14.058592944738452	12.163929318345977
11	26.058391518705083	27.7302275956813	29.03477588993326	17.176604995680357
12	24.562960585016736	25.58472561210696	31.49831069650919	18.35400310636711
13	23.614999315466214	26.178775046461748	32.47129285614247	17.734932781929565
14	20.693220282972106	29.984625528546633	30.062206024212042	19.259948164269225
15	20.113805708854404	29.159408248081338	32.4711354920539	18.255650551010355
16	23.404603529047048	27.883500217949265	30.601964848009892	18.10993140499379
17	22.810396730603696	26.763067907325137	29.568554878365426	20.857980483705735
18	23.658274439823185	26.19797346526739	29.714588752559134	20.429163342350297
19	23.02504134741427	28.856325013493972	28.353861478687392	19.764772160404362
20	24.310391222860595	27.669327693404398	28.63475637678628	19.385524706948726
21	23.626644258020455	29.0717564507474	28.779059246005705	18.52254004522644
22	23.25369136810765	25.220113018888412	29.14571757237568	22.38047804062826
23	19.922293613063736	31.030624625276765	28.659305174603322	20.387776587056177
24	21.206384575801497	28.423259041747116	30.059530834706333	20.31082554774505
25	21.526777860131652	29.51646736504849	28.074068129208506	20.88268664561135
26	20.327034048867844	30.005554952326552	28.99921160591626	20.668199392889345
27	20.565755371229752	34.11102665905024	26.52261557998895	18.80060238973105
28	20.274002351019483	29.950162793149627	29.765574717256072	20.010260138574818
29	21.75353951176218	27.644149439233068	29.138793552478564	21.463517496526187
30	22.67207369674996	27.842270826743714	29.22251124759823	20.263144228908097
31	23.269270412876157	28.929027222413684	26.57722091872302	21.224481445987138
32	22.17086907465195	29.18930742490979	27.940623382100465	20.699200118337792
33	22.484810431350702	29.656364039787935	26.83592748033342	21.02289804852794
34	21.87171994227885	28.46857989925551	28.644198222100524	21.015501936365112
35	21.284437163732616	28.704468668023146	28.012853498754463	21.99824066948978
36	23.728773551502904	29.404896226251793	26.914452160530256	19.951878061715046
37	23.00914757446862	28.9855209302106	27.219895856446186	20.785435638874596
38	21.713411669176626	30.666484124323922	26.385551458843782	21.23455274765567
39	23.591552066269163	27.58498054193045	26.97834198049	21.845125411310388
40	23.550165310975043	27.98657369596314	28.530581350152406	19.93267964290941
41	21.699563629382393	29.041227817564664	27.849981667083686	21.40922688596926
42	23.164308565799434	28.7510484382401	27.476871413082304	20.607771582878158
43	23.045183950751337	27.609844067924634	27.41833197213397	21.926640009190063
44	22.443738404233724	27.692145486247167	28.252676369736367	21.611439739782742
45	22.473480216973606	27.648870361890193	27.548314709293454	22.329334711842748
46	23.17154731387369	28.408624181510035	27.127365772366552	21.292462732249724
47	21.7809208631735	28.15967419339102	28.7952677471285	21.26413719630698
48	22.580802525378893	27.987360516405996	27.968004733511787	21.46383222470333
49	23.070834297188377	27.038612426412616	27.839123544972296	22.051429731426712
50	21.638978455282633	27.027754304301233	28.61555795798064	22.717709282435493
51	21.437237693734865	27.068039510975357	27.894987796414934	23.599734998874847
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1033.0
1	1216.5
2	1400.0
3	1723.0
4	2046.0
5	1523.0
6	1000.0
7	1017.5
8	1035.0
9	1096.0
10	1157.0
11	1285.0
12	1413.0
13	1413.0
14	1413.0
15	1419.0
16	1425.0
17	1480.0
18	1535.0
19	1680.0
20	1825.0
21	2008.0
22	2191.0
23	2497.0
24	2803.0
25	3896.0
26	6491.0
27	7993.0
28	9094.0
29	10195.0
30	12330.0
31	14465.0
32	16200.0
33	17935.0
34	20423.5
35	22912.0
36	25468.5
37	28025.0
38	29944.0
39	31863.0
40	33582.5
41	35302.0
42	38010.0
43	40718.0
44	43173.0
45	45628.0
46	50267.0
47	54906.0
48	58078.5
49	61251.0
50	59105.0
51	56959.0
52	51757.5
53	46556.0
54	40456.0
55	34356.0
56	30840.5
57	27325.0
58	24068.0
59	20811.0
60	19090.5
61	17370.0
62	15013.5
63	12657.0
64	10356.5
65	8056.0
66	6552.0
67	5048.0
68	4042.0
69	3036.0
70	2711.0
71	2386.0
72	1982.0
73	1578.0
74	1370.5
75	1071.5
76	980.0
77	667.0
78	354.0
79	255.0
80	156.0
81	134.0
82	112.0
83	83.5
84	55.0
85	39.0
86	23.0
87	14.0
88	5.0
89	5.5
90	6.0
91	7.0
92	8.0
93	5.5
94	3.0
95	5.5
96	8.0
97	4.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	635469.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.63439936193518
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.56622083943026	26.171906708250166
2	9.059764551312329	6.275590071905094
3	3.431890588987055	3.565844076763338
4	1.8548305683056172	2.569637706056877
5	1.2345509505583039	2.1378965327146493
6	0.9025095542133512	1.8754725797152378
7	0.6844208008055347	1.6593152342700064
8	0.6246215073723981	1.7306712589111661
9	0.5013209667787135	1.5626655514732868
>10	5.7689493776390774	42.757065874482095
>50	0.3379297876298561	7.243043691477981
>100	0.030315600997130758	1.7572977518936261
>500	0.002229088308612556	0.5362529735811057
>1k	4.458176617225111E-4	0.15733998850536904
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTCTTCTGC	1019	0.1603540062536489	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	879	0.13832303385373637	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	826	0.12998273715948377	No Hit
CTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTCTTCTGCT	712	0.11204323106241217	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.13014010124805458	0.0
2	0.0	0.0	0.0	0.5073418215522708	0.0
3	0.0	0.0	0.0	0.6733609349944687	0.0
4	0.0	0.0	0.0	0.9056303297249748	0.0
5	0.0	0.0	0.0	1.5550719232566812	0.0
6	0.0	0.0	0.0	2.109150879114481	0.0
7	0.0	0.0	0.0	2.6094113166810655	0.0
8	0.0	0.0	0.0	3.651318947108356	0.0
9	0.0	0.0	0.0	4.098390322738009	0.0
10	0.0	0.0	0.0	5.083174789014098	0.0
11	0.0	0.0	0.0	6.094396422170083	0.0
12	0.0	0.0	0.0	6.940228398238152	0.0
13	0.0	0.0	0.0	7.307830909139549	0.0
14	0.0	0.0	0.0	7.478885673416013	0.0
15	0.0	0.0	0.0	7.708479878640815	0.0
16	0.0	0.0	0.0	8.193948091881744	0.0
17	0.0	0.0	0.0	8.779499865453705	0.0
18	0.0	0.0	0.0	9.482759977276626	0.0
19	0.0	0.0	0.0	9.859961697580841	0.0
20	1.573640885708036E-4	0.0	0.0	10.221112280850836	0.0
21	1.573640885708036E-4	0.0	0.0	10.62302016306067	0.0
22	1.573640885708036E-4	0.0	0.0	11.071350451398889	0.0
23	1.573640885708036E-4	0.0	0.0	11.515431909345695	0.0
24	1.573640885708036E-4	0.0	0.0	11.847627500318662	0.0
25	1.573640885708036E-4	0.0	0.0	12.155116929386013	0.0
26	1.573640885708036E-4	0.0	0.0	12.438214924724889	0.0
27	3.147281771416072E-4	0.0	0.0	12.741770251577968	0.0
28	3.147281771416072E-4	0.0	0.0	13.073651114373794	0.0
29	3.147281771416072E-4	0.0	0.0	13.439522620300911	0.0
30	3.147281771416072E-4	0.0	0.0	13.880771524653445	0.0
31	4.720922657124108E-4	0.0	0.0	14.26946082342333	0.0
32	4.720922657124108E-4	0.0	0.0	14.64571835919612	0.0
33	4.720922657124108E-4	0.0	0.0	15.01158986512324	0.0
34	4.720922657124108E-4	0.0	0.0	15.376359822430363	0.0
35	4.720922657124108E-4	0.0	0.0	15.768039038883092	0.0
36	4.720922657124108E-4	0.0	0.0	16.12981907850737	0.0
37	4.720922657124108E-4	0.0	0.0	16.514259546885842	0.0
38	4.720922657124108E-4	0.0	0.0	16.888471349507213	0.0
39	4.720922657124108E-4	0.0	0.0	17.259221142180028	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTTCGC	20	7.030902E-4	45.000004	44
AAGCGTA	20	7.030902E-4	45.000004	22
CGTTAGA	20	7.030902E-4	45.000004	20
AGCGGAT	20	7.030902E-4	45.000004	4
CACTCCG	20	7.030902E-4	45.000004	35
CCATAGT	20	7.030902E-4	45.000004	25
CCGGTCG	20	7.030902E-4	45.000004	5
ATAACGC	20	7.030902E-4	45.000004	11
TATACGG	20	7.030902E-4	45.000004	2
GCGTAAG	40	6.8066583E-9	45.000004	1
CGCATCG	25	3.8888298E-5	45.0	21
TACGCAT	45	3.8380676E-10	45.0	16
CTACGCA	45	3.8380676E-10	45.0	15
CGAATAT	45	3.8380676E-10	45.0	14
GCTACGA	45	3.8380676E-10	45.0	10
CCAGTCG	25	3.8888298E-5	45.0	28
GATTACC	45	3.8380676E-10	45.0	9
TGATGCG	35	1.2107739E-7	45.0	1
GCGATTC	60	0.0	44.999996	9
CGTAAGG	95	0.0	42.631577	2
>>END_MODULE