FastQCFastQC Report
Sat 18 Jun 2016
SRR3554012_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554012_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences518843
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTGC19360.37313792418901287No Hit
CCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTGC17180.331121360411531No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13370.2576887420664825No Hit
CTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTGCT10260.19774768089768965No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8530.16440426101922934No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCC6020.11602739171579841No Hit
TCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTGC5720.11024529578311743No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTC5580.10754698434786632No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATCT302.1631913E-645.00000416
AACATCG302.1631913E-645.00000410
ACTGCGG502.1827873E-1145.02
CACATCG207.029704E-445.02
CGACCTT207.029704E-445.028
CACGCCG453.8380676E-1045.026
TCGATAG207.029704E-445.01
TGTGACG207.029704E-445.01
AACGGGT551.8189894E-1245.04
GCCGATA253.8878366E-545.09
GGACGTC207.029704E-445.08
TTAACGA253.8878366E-545.013
ACGTCAC207.029704E-445.010
CGGTATA207.029704E-445.038
ATCGGAG207.029704E-445.01
TATACGG253.8878366E-545.03
CATCCGG207.029704E-445.02
GTGCGTT207.029704E-445.01
TCGAACG253.8878366E-545.01
CGTAAGG900.042.52