##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554012_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	518843
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.653675582016138	31.0	31.0	34.0	30.0	34.0
2	31.760503659103044	31.0	31.0	34.0	30.0	34.0
3	31.775149708100525	31.0	31.0	34.0	30.0	34.0
4	35.43177030431171	37.0	35.0	37.0	33.0	37.0
5	35.292845812702495	37.0	35.0	37.0	33.0	37.0
6	35.25601000688069	37.0	35.0	37.0	32.0	37.0
7	35.66334902851151	37.0	35.0	37.0	35.0	37.0
8	35.56185975333579	37.0	35.0	37.0	35.0	37.0
9	37.28488772133382	39.0	37.0	39.0	34.0	39.0
10	36.55839242314149	38.0	35.0	39.0	32.0	39.0
11	36.529657333721374	38.0	35.0	39.0	32.0	39.0
12	36.565853254259956	39.0	35.0	39.0	32.0	39.0
13	36.492281865612526	39.0	35.0	39.0	32.0	39.0
14	37.55040542129315	39.0	36.0	41.0	32.0	41.0
15	37.68160888746692	39.0	36.0	41.0	33.0	41.0
16	37.72110445741775	39.0	36.0	41.0	33.0	41.0
17	37.67706801479446	39.0	36.0	41.0	33.0	41.0
18	37.65134154262465	39.0	36.0	41.0	33.0	41.0
19	37.57325240968848	39.0	36.0	41.0	32.0	41.0
20	37.50220779696363	39.0	36.0	41.0	32.0	41.0
21	37.41507546598875	39.0	36.0	41.0	32.0	41.0
22	37.41580015534564	39.0	36.0	41.0	32.0	41.0
23	36.985080265128374	39.0	35.0	40.0	31.0	41.0
24	37.117910427624544	39.0	35.0	41.0	31.0	41.0
25	37.140632137274665	39.0	35.0	41.0	31.0	41.0
26	37.17560803557146	39.0	35.0	41.0	32.0	41.0
27	35.13366085694516	39.0	35.0	40.0	27.0	41.0
28	35.856972147643894	39.0	35.0	40.0	25.0	41.0
29	36.45594717477156	39.0	35.0	40.0	30.0	41.0
30	36.69013747896763	39.0	35.0	40.0	30.0	41.0
31	36.705143945278245	39.0	35.0	40.0	30.0	41.0
32	36.49720050188593	39.0	35.0	40.0	30.0	41.0
33	36.54100758803723	39.0	35.0	40.0	30.0	41.0
34	36.49052989054493	39.0	35.0	40.0	30.0	41.0
35	36.469155409247115	39.0	35.0	40.0	30.0	41.0
36	36.381202020649795	39.0	35.0	40.0	30.0	41.0
37	36.293331508760836	39.0	35.0	40.0	30.0	41.0
38	36.24899439714904	38.0	35.0	40.0	30.0	41.0
39	36.20499457446665	38.0	35.0	40.0	30.0	41.0
40	36.14067068458088	38.0	35.0	40.0	30.0	41.0
41	36.077308549985254	38.0	35.0	40.0	29.0	41.0
42	36.0037679991828	38.0	35.0	40.0	29.0	41.0
43	35.881008320436045	38.0	35.0	40.0	29.0	41.0
44	35.78526837598272	38.0	34.0	40.0	28.0	41.0
45	35.72185034779307	38.0	34.0	40.0	28.0	41.0
46	35.552702840743734	38.0	34.0	40.0	28.0	41.0
47	35.4452676435839	38.0	34.0	40.0	27.0	41.0
48	35.38057369955844	38.0	34.0	40.0	27.0	41.0
49	35.350726520353945	38.0	34.0	40.0	27.0	41.0
50	35.237225904560724	38.0	34.0	40.0	27.0	41.0
51	33.546903398523256	36.0	31.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	1.0
12	4.0
13	4.0
14	9.0
15	22.0
16	37.0
17	96.0
18	167.0
19	343.0
20	628.0
21	1017.0
22	1456.0
23	1998.0
24	2877.0
25	3861.0
26	4838.0
27	5809.0
28	7260.0
29	9178.0
30	11783.0
31	15259.0
32	19585.0
33	25518.0
34	36602.0
35	45479.0
36	49014.0
37	66996.0
38	98424.0
39	110559.0
40	16.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.474808371703965	18.03975383690249	23.42346335982176	15.061974431571787
2	30.11778129414871	26.25437752846237	25.830357160065766	17.79748401732316
3	31.251072096954186	25.175052954361917	27.301515101870894	16.272359846813007
4	27.005664526648715	27.29303469450296	26.497803767228234	19.203497011620087
5	23.75362103757784	31.57641136143303	25.768874206648253	18.90109339434087
6	23.104098927806678	36.73442640644665	24.562343522028822	15.599131143717848
7	81.02084831056794	6.208429139450662	8.417575258796978	4.353147291184424
8	82.17553286832432	5.017124640787291	7.323024498740467	5.484317992147913
9	75.87709576885493	8.16085019938594	10.202122800153418	5.759931231605708
10	34.095670559302135	31.665070165734143	19.139123010236236	15.100136264727478
11	26.582607840907556	27.042862677148964	26.952661980599142	19.421867501344337
12	23.755548402888735	24.01824829476354	31.50933904861394	20.71686425373379
13	22.022846988395333	26.372139548957968	33.919702106417546	17.685311356229146
14	20.647479102541617	29.177034285901517	30.18543181656108	19.99005479499579
15	19.339376266038087	27.734594087228697	34.63514010982128	18.290889536911937
16	21.574734553612558	26.869014326106356	31.49642570103095	20.05982541925014
17	21.97215728071883	26.579909529472307	28.933029837542378	22.514903352266487
18	22.851806808610696	25.83459736374973	31.12540787868392	20.188187948955658
19	21.9274424055061	28.696542113895724	28.552760661703058	20.82325481889512
20	23.314759956287354	27.401738098037363	28.930909735700393	20.352592209974887
21	22.82289632894729	29.236204400945955	28.988923431558295	18.951975838548464
22	20.93928221061092	27.130557798794623	28.22703592416203	23.703124066432427
23	19.173044639707964	29.693953662283196	28.24014200827611	22.892859689732735
24	20.17315449953069	26.997376855811872	29.869536642105608	22.95993200255183
25	19.550615504112034	29.664079499964345	27.48750585437213	23.29779914155149
26	20.723224559259737	28.60287215978629	28.65279092133844	22.02111235961553
27	21.600561248778533	31.59452859535544	26.85610097852337	19.948809177342664
28	20.028987574275842	28.316851147649675	30.044734148865842	21.609427129208644
29	22.62630506723614	25.464350487527053	29.759484082853582	22.149860362383226
30	25.17967863110806	25.552816555297074	28.53984731412007	20.727657499474795
31	23.412284641018573	26.692660400159586	27.692770259982307	22.202284698839534
32	23.855000452930845	26.371561339364703	28.727765431932202	21.045672775772246
33	24.178605088629894	25.14421510938762	28.520766397542225	22.156413404440265
34	22.190142297380902	24.998699028415146	31.41354899266252	21.39760968154143
35	22.922733852051582	24.369992464001637	29.921382768968645	22.785890914978136
36	24.87496217545577	24.636932559560407	30.62043816722978	19.86766709775404
37	21.44579381431377	25.272577639093136	33.17920835397221	20.10242019262089
38	21.246696977698456	25.98897932515231	30.39011801257799	22.374205684571248
39	22.663888690798565	23.752464618391304	32.06075826406061	21.522888426749518
40	23.631618813398273	23.872924950322158	30.60694661005352	21.888509626226046
41	20.866427801859135	24.41933301596051	30.028929753316515	24.68530942886384
42	23.073839292425646	23.999360114716783	30.461237792549962	22.465562800307605
43	22.967641463795406	24.233534999990365	31.236231384060304	21.56259215215393
44	22.33122543813832	24.952827734015877	29.849492042872317	22.866454784973488
45	22.71072366785328	24.241051724702846	29.81518494033841	23.233039667105462
46	23.91513425063073	25.446233253604657	28.693265592867206	21.94536690289741
47	21.477980815005697	25.084659521281004	31.482934143854692	21.95442551985861
48	21.512095181008515	24.098426691696716	30.709289708061977	23.680188419232792
49	22.557498125637235	22.64095304359893	31.651964081620065	23.149584749143767
50	21.559122894594317	23.23072682873239	30.780409488033953	24.429740788639336
51	20.92077950362634	23.245374805095185	28.745304456261334	27.088541235017143
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1615.0
1	1702.5
2	1790.0
3	1646.0
4	1502.0
5	1140.0
6	778.0
7	796.5
8	815.0
9	889.5
10	964.0
11	1039.5
12	1115.0
13	1081.0
14	1047.0
15	1202.5
16	1358.0
17	1296.5
18	1235.0
19	1393.5
20	1552.0
21	1627.0
22	1702.0
23	2078.5
24	2455.0
25	2824.0
26	3724.0
27	4255.0
28	5234.0
29	6213.0
30	7517.5
31	8822.0
32	10178.0
33	11534.0
34	12922.0
35	14310.0
36	15847.0
37	17384.0
38	18440.0
39	19496.0
40	21574.5
41	23653.0
42	27532.0
43	31411.0
44	35698.0
45	39985.0
46	45484.0
47	50983.0
48	56264.5
49	61546.0
50	60202.0
51	58858.0
52	50822.5
53	42787.0
54	37385.5
55	31984.0
56	27046.0
57	22108.0
58	18847.5
59	15587.0
60	13886.0
61	12185.0
62	10909.0
63	9633.0
64	7898.0
65	6163.0
66	4788.5
67	3414.0
68	2838.0
69	2262.0
70	1767.0
71	1272.0
72	1008.0
73	744.0
74	646.0
75	390.5
76	233.0
77	195.0
78	157.0
79	133.0
80	109.0
81	76.0
82	43.0
83	33.0
84	23.0
85	14.5
86	6.0
87	4.5
88	3.0
89	2.5
90	2.0
91	2.0
92	2.0
93	2.0
94	2.0
95	2.0
96	2.0
97	2.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	518843.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.00352928604943
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.0537068761451	28.90309276580199
2	9.881292317963773	7.5104796397950295
3	3.5558375735234944	4.054031320855051
4	1.8879639494857898	2.86997172981155
5	1.1457230760421955	2.1770760237036106
6	0.8482264659336454	1.9341359603586898
7	0.6275016291190798	1.6693093577489473
8	0.5161956902354194	1.569380642495541
9	0.4434865284523913	1.516864794480796
>10	4.763360163503944	38.104713974051805
>50	0.23753504555088933	5.709446501408502
>100	0.035153177988487036	2.3398119436836753
>500	0.002008753027913545	0.4933453404912153
>1k	0.002008753027913545	1.148340005313595
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTGC	1936	0.37313792418901287	No Hit
CCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTGC	1718	0.331121360411531	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1337	0.2576887420664825	No Hit
CTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTGCT	1026	0.19774768089768965	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	853	0.16440426101922934	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCC	602	0.11602739171579841	No Hit
TCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTGC	572	0.11024529578311743	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTC	558	0.10754698434786632	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.8547306217873227E-4	0.0	0.0	0.22126153769059234	0.0
2	3.8547306217873227E-4	0.0	0.0	1.2119273074899344	0.0
3	3.8547306217873227E-4	0.0	0.0	1.612819292155816	0.0
4	3.8547306217873227E-4	0.0	0.0	2.1536379983925773	0.0
5	3.8547306217873227E-4	0.0	0.0	3.6440695933066456	0.0
6	3.8547306217873227E-4	0.0	0.0	4.792779318599268	0.0
7	3.8547306217873227E-4	0.0	0.0	5.805031579880619	0.0
8	3.8547306217873227E-4	0.0	0.0	7.7138941837897015	0.0
9	3.8547306217873227E-4	0.0	0.0	8.5476724172823	0.0
10	3.8547306217873227E-4	0.0	0.0	10.325088706988433	0.0
11	3.8547306217873227E-4	0.0	0.0	12.164758896236433	0.0
12	3.8547306217873227E-4	0.0	0.0	13.729779528682087	0.0
13	3.8547306217873227E-4	0.0	0.0	14.304712600921667	0.0
14	3.8547306217873227E-4	0.0	0.0	14.5198065696174	0.0
15	3.8547306217873227E-4	0.0	0.0	14.891402601557697	0.0
16	3.8547306217873227E-4	0.0	0.0	15.703979816630465	0.0
17	3.8547306217873227E-4	0.0	0.0	16.65378544183886	0.0
18	3.8547306217873227E-4	0.0	0.0	17.754696507421322	0.0
19	3.8547306217873227E-4	0.0	0.0	18.33541167559358	0.0
20	3.8547306217873227E-4	0.0	0.0	18.908417382522266	0.0
21	3.8547306217873227E-4	0.0	0.0	19.51804303035793	0.0
22	3.8547306217873227E-4	0.0	0.0	20.18067122424317	0.0
23	3.8547306217873227E-4	0.0	0.0	20.800704644757662	0.0
24	3.8547306217873227E-4	0.0	0.0	21.30952908683359	0.0
25	0.001156419186536197	0.0	0.0	21.742800808722485	0.0
26	0.001156419186536197	0.0	0.0	22.184552937979312	0.0
27	0.001156419186536197	0.0	0.0	22.66196132548767	0.0
28	0.001156419186536197	0.0	0.0	23.14341718014891	0.0
29	0.001156419186536197	0.0	0.0	23.67806831739081	0.0
30	0.001156419186536197	0.0	0.0	24.2799845039829	0.0
31	0.001156419186536197	0.0	0.0	24.813671958569355	0.0
32	0.001156419186536197	0.0	0.0	25.311124945311008	0.0
33	0.001156419186536197	0.0	0.0	25.777161877485096	0.0
34	0.001156419186536197	0.0	0.0	26.260737833988316	0.0
35	0.001156419186536197	0.0	0.0	26.76724943769117	0.0
36	0.001156419186536197	0.0	0.0	27.25718569972034	0.0
37	0.001156419186536197	0.0	0.0	27.741918075410094	0.0
38	0.001156419186536197	0.0	0.0	28.229156026004013	0.0
39	0.001349155717625563	0.0	0.0	28.714852084349214	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGATCT	30	2.1631913E-6	45.000004	16
AACATCG	30	2.1631913E-6	45.000004	10
ACTGCGG	50	2.1827873E-11	45.0	2
CACATCG	20	7.029704E-4	45.0	2
CGACCTT	20	7.029704E-4	45.0	28
CACGCCG	45	3.8380676E-10	45.0	26
TCGATAG	20	7.029704E-4	45.0	1
TGTGACG	20	7.029704E-4	45.0	1
AACGGGT	55	1.8189894E-12	45.0	4
GCCGATA	25	3.8878366E-5	45.0	9
GGACGTC	20	7.029704E-4	45.0	8
TTAACGA	25	3.8878366E-5	45.0	13
ACGTCAC	20	7.029704E-4	45.0	10
CGGTATA	20	7.029704E-4	45.0	38
ATCGGAG	20	7.029704E-4	45.0	1
TATACGG	25	3.8878366E-5	45.0	3
CATCCGG	20	7.029704E-4	45.0	2
GTGCGTT	20	7.029704E-4	45.0	1
TCGAACG	25	3.8878366E-5	45.0	1
CGTAAGG	90	0.0	42.5	2
>>END_MODULE