FastQCFastQC Report
Sat 18 Jun 2016
SRR3554010_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554010_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences733944
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11990.16336396237315107No Hit
GCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCTGC10520.14333518633574224TruSeq Adapter, Index 16 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9440.12862016720621738No Hit
GCTAAAGGTGTAATTTGAAATGGCCTTCGGGTAAATGCAAGATACTTAACT8240.11227014595118973No Hit
CTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCTGCT7630.1039588851465507TruSeq Adapter, Index 13 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTACCG302.1644246E-645.00000411
TCGTTTA406.8084773E-945.038
GTCGAAC207.0316176E-445.045
ACGTTGA351.2111195E-745.016
ATTACGG351.2111195E-745.02
CTCGCAC253.8894228E-545.019
ATCGGCC207.0316176E-445.018
TCGACGA253.8894228E-545.042
TCGGTAT207.0316176E-445.015
TCGGTAC253.8894228E-545.035
TACGTAG253.8894228E-545.01
AACACGT2550.045.041
CACGACC2700.043.33333227
TTTAGCG1000.042.751
CGAATAT750.042.014
GCTACGA750.042.010
GCGAGAC2800.041.78571321
GACACGA2850.041.0526325
ACACGTG2800.040.9821442
TTAACGG556.002665E-1140.9090922
Produced by FastQC (version 0.11.5)