##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554010_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	733944
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.87765687845394	31.0	31.0	34.0	30.0	34.0
2	31.987360071068093	33.0	31.0	34.0	30.0	34.0
3	32.01718250983726	33.0	31.0	34.0	30.0	34.0
4	35.650950208735274	37.0	35.0	37.0	33.0	37.0
5	35.501637727129044	37.0	35.0	37.0	33.0	37.0
6	35.44627791766129	37.0	35.0	37.0	33.0	37.0
7	35.75168132718573	37.0	35.0	37.0	35.0	37.0
8	35.641196058554875	37.0	35.0	37.0	35.0	37.0
9	37.37347672301974	39.0	37.0	39.0	34.0	39.0
10	36.782273579455655	39.0	37.0	39.0	32.0	39.0
11	36.72476782969818	39.0	35.0	39.0	32.0	39.0
12	36.618488604035186	39.0	35.0	39.0	32.0	39.0
13	36.484774042706256	39.0	35.0	39.0	32.0	39.0
14	37.578360201868264	39.0	36.0	41.0	33.0	41.0
15	37.72334401534722	40.0	36.0	41.0	33.0	41.0
16	37.77544880808345	40.0	36.0	41.0	33.0	41.0
17	37.702655788452525	39.0	36.0	41.0	33.0	41.0
18	37.697915644790335	39.0	36.0	41.0	33.0	41.0
19	37.63106040787853	39.0	36.0	41.0	33.0	41.0
20	37.54535223395791	39.0	36.0	41.0	32.0	41.0
21	37.44031560991029	39.0	35.0	41.0	32.0	41.0
22	37.444683790588925	39.0	35.0	41.0	32.0	41.0
23	36.946256117632956	39.0	35.0	41.0	32.0	41.0
24	37.05117147902292	39.0	35.0	41.0	31.0	41.0
25	37.04432490762238	39.0	35.0	41.0	31.0	41.0
26	37.071640343132444	39.0	35.0	41.0	31.0	41.0
27	34.5647610716894	39.0	35.0	41.0	24.0	41.0
28	35.48126015063819	39.0	35.0	40.0	23.0	41.0
29	36.204669566070436	39.0	35.0	40.0	29.0	41.0
30	36.536816160361006	38.0	35.0	40.0	30.0	41.0
31	36.60765262744842	39.0	35.0	40.0	30.0	41.0
32	36.37839262941042	38.0	35.0	40.0	30.0	41.0
33	36.430620047306064	39.0	35.0	40.0	30.0	41.0
34	36.38100590780768	39.0	35.0	40.0	30.0	41.0
35	36.304959233947	38.0	35.0	40.0	30.0	41.0
36	36.234515167369715	38.0	35.0	40.0	30.0	41.0
37	36.21361302769694	38.0	35.0	40.0	30.0	41.0
38	36.11840957893245	38.0	35.0	40.0	30.0	41.0
39	36.036817522862776	38.0	35.0	40.0	29.0	41.0
40	35.919495220343784	38.0	35.0	40.0	29.0	41.0
41	35.82914227788496	38.0	35.0	40.0	29.0	41.0
42	35.744066304786195	38.0	34.0	40.0	29.0	41.0
43	35.65745751719477	38.0	34.0	40.0	28.0	41.0
44	35.545124696162105	38.0	34.0	40.0	28.0	41.0
45	35.47001406101828	38.0	34.0	40.0	28.0	41.0
46	35.31383320798317	38.0	34.0	40.0	27.0	41.0
47	35.20871347132751	37.0	34.0	40.0	27.0	41.0
48	35.135645226338795	37.0	34.0	40.0	27.0	41.0
49	35.08654747501172	37.0	34.0	40.0	27.0	41.0
50	34.97877358490566	37.0	34.0	40.0	27.0	41.0
51	33.42438796420436	35.0	31.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	1.0
10	3.0
11	4.0
12	7.0
13	7.0
14	24.0
15	37.0
16	82.0
17	164.0
18	312.0
19	567.0
20	989.0
21	1522.0
22	2281.0
23	3176.0
24	4311.0
25	5327.0
26	6868.0
27	8726.0
28	10537.0
29	13197.0
30	16368.0
31	21009.0
32	27202.0
33	36798.0
34	56019.0
35	66728.0
36	69242.0
37	94404.0
38	131444.0
39	156548.0
40	39.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.93340091342119	17.481033975344168	23.08350500855651	14.502060102678133
2	29.011886465452406	25.873363635372726	27.0844914598389	18.030258439335974
3	33.61060244378318	25.20941652224148	25.181485235930808	15.998495798044537
4	28.54931711411225	26.768936049616865	25.133388923405604	19.548357912865285
5	24.968389958906947	31.944399027718735	23.541305603697285	19.545905409677033
6	25.459299347089154	35.9749517674373	24.124047611261894	14.441701274211658
7	82.59853612809697	6.584153559399628	6.780353814459959	4.036956498043447
8	84.4207459969698	5.213613027696936	6.344625748013473	4.021015227319795
9	77.38860185518242	8.206348168252617	8.667282517467273	5.737767459097697
10	39.56132892972761	28.00431640561133	15.905164426713755	16.529190237947308
11	31.786212572076344	27.347863052221967	21.58502556053323	19.28089881516846
12	27.18640659232857	24.016001220801584	28.46061824880372	20.33697393806612
13	24.0236312307206	26.418228093696523	29.66493356441363	19.893207111169247
14	20.763300742290966	28.821544968008457	29.221166737516757	21.193987552183817
15	20.052892318760016	26.75054227570496	32.974995367493975	20.22157003804105
16	22.813729657848555	25.55644572337944	30.59225226992795	21.03757234884405
17	22.650093195121155	24.714555878922642	27.89409001231701	24.741260913639188
18	23.829474728317145	25.64991334488735	28.211007924310298	22.3096040024852
19	25.62661456459893	27.546243310116303	25.739429711258627	21.08771241402614
20	25.752373478085524	26.23606160688009	25.923231200200558	22.08833371483383
21	25.674983377478387	27.536569547540413	25.792703530514586	20.99574354446661
22	23.19890890858158	25.929907458879697	25.581243255616233	25.289940376922488
23	22.631290670677874	28.66553851520007	25.388313004806907	23.31485780931515
24	22.404979126472867	26.42599435379266	28.26264674144076	22.90637977829371
25	23.13037506948759	27.767922348299052	25.609992042989653	23.4917105392237
26	21.813380857395114	28.637743479066526	25.971736263257146	23.57713940028122
27	21.843219646185542	32.04222665489465	25.446083079908004	20.668470619011806
28	21.363755272881853	28.56089837916789	27.74462356801064	22.330722779939613
29	22.345982799777637	27.72023478630522	26.132783972619166	23.800998441297974
30	24.147755142081685	27.39309811102754	26.632140871783132	21.827005875107638
31	24.69575335447936	28.36537937499319	24.06409753332679	22.87476973720066
32	24.47216681381686	28.718948584633157	24.61863575422648	22.190248847323502
33	24.059056276773163	27.064598933978612	24.511134364474675	24.365210424773554
34	21.995819844565798	29.043905257076837	25.405889277656062	23.554385620701307
35	23.165118864654524	27.291864229423496	24.955854942611424	24.587161963310553
36	24.04679376083189	29.93620766706997	24.314389108705843	21.702609463392303
37	22.856103462934502	29.080965305254896	24.910074883097348	23.152856348713254
38	22.42882290746978	29.212582976357872	24.78908472581014	23.56950939036221
39	23.467730508049662	26.37026803134844	24.716735881756648	25.445265578845248
40	23.97648866943527	26.619469605310485	26.764031043240355	22.640010682013887
41	20.73223570190641	27.06037517848773	26.379533043392954	25.827856076212903
42	21.92524225281493	28.26673424675452	26.068473889016058	23.739549611414496
43	22.610444393577712	25.880448644583236	26.65857340614543	24.850533555693623
44	21.874284686570093	27.097980227374297	26.78651232246602	24.241222763589594
45	23.53449309484102	26.613747097871226	25.493498141547583	24.358261665740166
46	24.148163892613063	27.261480439924572	25.9920375396488	22.598318127813567
47	22.247065171184722	26.51918947494632	27.178640332232433	24.05510502163653
48	21.572899294769083	27.724049791264726	26.902869973730965	23.800180940235222
49	22.321457767895097	26.901779972313967	27.016775121807658	23.75998713798328
50	20.99656104552936	26.34138299379789	27.606193388051402	25.055862572621347
51	21.606280588164765	25.81177855531212	26.29682918587794	26.285111670645172
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1526.0
1	1929.0
2	2332.0
3	2408.5
4	2485.0
5	1927.5
6	1370.0
7	1403.0
8	1436.0
9	1485.0
10	1534.0
11	1633.5
12	1733.0
13	1671.5
14	1610.0
15	1665.0
16	1720.0
17	1733.0
18	1746.0
19	1882.0
20	2018.0
21	2370.5
22	2723.0
23	3201.5
24	3680.0
25	4237.5
26	5702.5
27	6610.0
28	8453.5
29	10297.0
30	12586.5
31	14876.0
32	15214.5
33	15553.0
34	17470.5
35	19388.0
36	20591.0
37	21794.0
38	23200.5
39	24607.0
40	28105.5
41	31604.0
42	35268.5
43	38933.0
44	42570.5
45	46208.0
46	51376.0
47	56544.0
48	60090.0
49	63636.0
50	60769.5
51	57903.0
52	53757.5
53	49612.0
54	45657.5
55	41703.0
56	39426.0
57	37149.0
58	35402.5
59	33656.0
60	32589.0
61	31522.0
62	29010.5
63	26499.0
64	24131.0
65	21763.0
66	18075.5
67	14388.0
68	13301.5
69	12215.0
70	10873.5
71	9532.0
72	8261.0
73	6990.0
74	5612.0
75	3577.5
76	2921.0
77	2158.0
78	1395.0
79	1133.5
80	872.0
81	665.0
82	458.0
83	324.0
84	190.0
85	126.5
86	63.0
87	58.0
88	53.0
89	47.5
90	42.0
91	23.0
92	4.0
93	4.0
94	4.0
95	4.5
96	5.0
97	5.0
98	5.0
99	6.5
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	733944.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.65589499716834
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.89312495695711	21.74785419773539
2	9.379057447035331	5.375305707493084
3	3.4703133803204715	2.983348075011953
4	1.8403598305029985	2.109486322396018
5	1.1013410065970628	1.5779956120560557
6	0.7479697043007305	1.286024478450287
7	0.5482838482487361	1.0998095068841414
8	0.44989609528609176	1.0313740212923428
9	0.37596947836102645	0.9696367704648354
>10	4.953310604762443	34.6554373400879
>50	1.1375569069662248	21.520139893654072
>100	0.0957744981507071	4.19357514766571
>500	0.006103276842937217	1.1471757493871069
>1k	9.389656681441873E-4	0.30283717742117716
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1199	0.16336396237315107	No Hit
GCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCTGC	1052	0.14333518633574224	TruSeq Adapter, Index 16 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	944	0.12862016720621738	No Hit
GCTAAAGGTGTAATTTGAAATGGCCTTCGGGTAAATGCAAGATACTTAACT	824	0.11227014595118973	No Hit
CTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCTGCT	763	0.1039588851465507	TruSeq Adapter, Index 13 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.3625017712523026E-4	0.0	0.0	0.1125426463054402	0.0
2	1.3625017712523026E-4	0.0	0.0	0.4703356114362949	0.0
3	1.3625017712523026E-4	0.0	0.0	0.6432370862082121	0.0
4	1.3625017712523026E-4	0.0	0.0	0.8805848947603632	0.0
5	1.3625017712523026E-4	0.0	0.0	1.54739326161124	0.0
6	1.3625017712523026E-4	0.0	0.0	2.163789062925782	0.0
7	1.3625017712523026E-4	0.0	0.0	2.6927122505259256	0.0
8	1.3625017712523026E-4	0.0	0.0	3.643738486860033	0.0
9	1.3625017712523026E-4	0.0	0.0	4.119796605735587	0.0
10	1.3625017712523026E-4	0.0	0.0	5.056244073117295	0.0
11	2.725003542504605E-4	0.0	0.0	6.001547802012142	0.0
12	2.725003542504605E-4	0.0	0.0	6.771906303478194	0.0
13	2.725003542504605E-4	0.0	0.0	7.085826711574725	0.0
14	2.725003542504605E-4	0.0	0.0	7.202593113371047	0.0
15	2.725003542504605E-4	0.0	0.0	7.405469627110516	0.0
16	2.725003542504605E-4	0.0	0.0	7.8533239593211475	0.0
17	4.087505313756908E-4	0.0	0.0	8.34259834537785	0.0
18	4.087505313756908E-4	0.0	0.0	9.018807974450366	0.0
19	4.087505313756908E-4	0.0	0.0	9.291717079232203	0.0
20	4.087505313756908E-4	0.0	0.0	9.594737473158714	0.0
21	4.087505313756908E-4	0.0	0.0	9.959479197322956	0.0
22	4.087505313756908E-4	0.0	0.0	10.319860915819191	0.0
23	5.45000708500921E-4	0.0	0.0	10.700407660529958	0.0
24	5.45000708500921E-4	0.0	0.0	10.98149177593931	0.0
25	0.0012262515941270724	0.0	0.0	11.244999618499504	0.0
26	0.0012262515941270724	0.0	0.0	11.480303674394778	0.0
27	0.0012262515941270724	0.0	0.0	11.73754400880721	0.0
28	0.0012262515941270724	0.0	0.0	11.999280599064779	0.0
29	0.0012262515941270724	0.0	0.0	12.291400978821272	0.0
30	0.0013625017712523026	0.0	0.0	12.63911143084486	0.0
31	0.0013625017712523026	0.0	0.0	12.94703683114788	0.0
32	0.0013625017712523026	0.0	0.0	13.246650970646261	0.0
33	0.001498751948377533	0.0	0.0	13.548036362447272	0.0
34	0.001498751948377533	0.0	0.0	13.869314280108565	0.0
35	0.001498751948377533	0.0	0.0	14.229968498959048	0.0
36	0.001498751948377533	0.0	0.0	14.541436403867325	0.0
37	0.001498751948377533	0.0	0.0	14.87061683180188	0.0
38	0.001498751948377533	0.0	0.0	15.185082240606913	0.0
39	0.001498751948377533	0.0	0.0	15.515897670666972	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTACCG	30	2.1644246E-6	45.000004	11
TCGTTTA	40	6.8084773E-9	45.0	38
GTCGAAC	20	7.0316176E-4	45.0	45
ACGTTGA	35	1.2111195E-7	45.0	16
ATTACGG	35	1.2111195E-7	45.0	2
CTCGCAC	25	3.8894228E-5	45.0	19
ATCGGCC	20	7.0316176E-4	45.0	18
TCGACGA	25	3.8894228E-5	45.0	42
TCGGTAT	20	7.0316176E-4	45.0	15
TCGGTAC	25	3.8894228E-5	45.0	35
TACGTAG	25	3.8894228E-5	45.0	1
AACACGT	255	0.0	45.0	41
CACGACC	270	0.0	43.333332	27
TTTAGCG	100	0.0	42.75	1
CGAATAT	75	0.0	42.0	14
GCTACGA	75	0.0	42.0	10
GCGAGAC	280	0.0	41.785713	21
GACACGA	285	0.0	41.05263	25
ACACGTG	280	0.0	40.98214	42
TTAACGG	55	6.002665E-11	40.909092	2
>>END_MODULE