FastQCFastQC Report
Sat 18 Jun 2016
SRR3554009_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554009_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences962803
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTGC16020.1663891782638816TruSeq Adapter, Index 21 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTGC13450.13969628262479447TruSeq Adapter, Index 21 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11480.11923519141506622No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11080.11508065512882697No Hit
CTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTGCT10590.10999134817818391TruSeq Adapter, Index 15 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCACG253.8903323E-545.023
CGCATCG551.8189894E-1245.021
ATTACGA253.8903323E-545.030
CATTACG253.8903323E-545.029
TACGAGT253.8903323E-545.032
CAATGCG207.0327154E-445.01
AGTACGG950.045.02
GTACGAT453.8380676E-1045.010
CAGACGA207.0327154E-445.025
CGACCTA302.165134E-644.9999969
TTACGCG302.165134E-644.9999961
TAGGACG302.165134E-644.9999961
TAATGCG302.165134E-644.9999961
TTAACGG750.042.02
GCTACGA1400.041.78571310
TTCGTAC603.6379788E-1241.24999633
TCGTACA603.6379788E-1241.24999634
TACGATT501.0804797E-940.511
CTACGCA950.040.26315715
CGAATAT1500.039.014
Produced by FastQC (version 0.11.5)