##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554009_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	962803
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.81147752967118	31.0	31.0	34.0	30.0	34.0
2	31.931950772899544	33.0	31.0	34.0	30.0	34.0
3	31.93848689711187	33.0	31.0	34.0	30.0	34.0
4	35.59216579092504	37.0	35.0	37.0	33.0	37.0
5	35.4400505607066	37.0	35.0	37.0	33.0	37.0
6	35.389640456043445	37.0	35.0	37.0	33.0	37.0
7	35.72650687627687	37.0	35.0	37.0	35.0	37.0
8	35.616946561238386	37.0	35.0	37.0	35.0	37.0
9	37.351265004367455	39.0	37.0	39.0	34.0	39.0
10	36.750956322321386	39.0	37.0	39.0	32.0	39.0
11	36.70529173673119	39.0	35.0	39.0	32.0	39.0
12	36.6159089658009	39.0	35.0	39.0	32.0	39.0
13	36.53510531230169	39.0	35.0	39.0	32.0	39.0
14	37.61675337530107	39.0	36.0	41.0	33.0	41.0
15	37.745340427896465	40.0	36.0	41.0	33.0	41.0
16	37.76420202263599	40.0	36.0	41.0	33.0	41.0
17	37.70813759408726	39.0	36.0	41.0	33.0	41.0
18	37.666232863836115	39.0	36.0	41.0	33.0	41.0
19	37.620914143391744	39.0	36.0	41.0	33.0	41.0
20	37.553943018457566	39.0	36.0	41.0	32.0	41.0
21	37.45711116396605	39.0	36.0	41.0	32.0	41.0
22	37.450939600312836	39.0	36.0	41.0	32.0	41.0
23	37.00549437423855	39.0	35.0	41.0	32.0	41.0
24	37.10245709662309	39.0	35.0	41.0	31.0	41.0
25	37.089137653289406	39.0	35.0	41.0	31.0	41.0
26	37.115942721408224	39.0	35.0	41.0	31.0	41.0
27	34.84072234922409	39.0	35.0	41.0	25.0	41.0
28	35.65860513521458	39.0	35.0	40.0	24.0	41.0
29	36.29926578957481	39.0	35.0	40.0	30.0	41.0
30	36.569942137695875	39.0	35.0	40.0	30.0	41.0
31	36.65228089235285	39.0	35.0	40.0	30.0	41.0
32	36.40876690247122	39.0	35.0	40.0	30.0	41.0
33	36.4501190793963	39.0	35.0	40.0	30.0	41.0
34	36.40256106389365	39.0	35.0	40.0	30.0	41.0
35	36.28448706537059	38.0	35.0	40.0	30.0	41.0
36	36.2037446912816	38.0	35.0	40.0	30.0	41.0
37	36.164342030508834	38.0	35.0	40.0	30.0	41.0
38	36.04485964418474	38.0	35.0	40.0	30.0	41.0
39	35.94679493105028	38.0	35.0	40.0	29.0	41.0
40	35.80820167780948	38.0	35.0	40.0	29.0	41.0
41	35.690750859729356	38.0	34.0	40.0	28.0	41.0
42	35.64035321867506	38.0	34.0	40.0	28.0	41.0
43	35.56897828527747	38.0	34.0	40.0	28.0	41.0
44	35.504365898319804	38.0	34.0	40.0	28.0	41.0
45	35.43680379059891	38.0	34.0	40.0	28.0	41.0
46	35.26829995336533	38.0	34.0	40.0	27.0	41.0
47	35.15715156683143	37.0	34.0	40.0	27.0	41.0
48	35.03709481586576	37.0	34.0	40.0	26.0	41.0
49	34.98503224439475	37.0	34.0	40.0	27.0	41.0
50	34.868913993828436	37.0	34.0	40.0	26.0	41.0
51	33.157810060832794	35.0	31.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	5.0
11	7.0
12	8.0
13	23.0
14	36.0
15	52.0
16	112.0
17	189.0
18	432.0
19	754.0
20	1299.0
21	1980.0
22	3039.0
23	4090.0
24	5379.0
25	7085.0
26	9254.0
27	11292.0
28	14274.0
29	17612.0
30	22113.0
31	28160.0
32	36217.0
33	48286.0
34	72099.0
35	85067.0
36	92720.0
37	125782.0
38	177121.0
39	198273.0
40	41.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.0398503120576	17.75981171641551	23.36511207380949	14.835225897717393
2	30.551732805153286	25.781078787664768	26.03419391090389	17.632994496278055
3	33.325405093253764	24.270593257395333	25.54343931209188	16.860562337259026
4	28.67357081355168	26.744308025629334	24.61676999344622	19.965351167372763
5	24.604410247994657	30.898636585054263	23.957860538448674	20.539092628502402
6	24.9480942622738	36.12068097004268	23.968039152349963	14.963185615333563
7	81.80946673410864	6.131472378046183	7.425610431209708	4.63345045663547
8	83.23779630931769	4.97121425670672	7.020750870115694	4.7702385638598965
9	76.55854832193086	7.524488394822201	9.774273657227907	6.14268962601903
10	39.387392851912594	26.217097370905574	16.927242644653166	17.46826713252867
11	30.318871046309575	26.42295464388873	22.677120864808273	20.58105344499342
12	26.991710661474883	23.457862096399783	29.606368073219546	19.94405916890579
13	24.982992367078207	23.87040754962334	30.463552772477858	20.683047310820594
14	20.915909069664306	27.748563309420515	28.820018217641614	22.51550940327357
15	20.571809601756538	25.97281063727471	32.45658769239398	20.998792068574776
16	23.080734065016415	24.526201102406205	30.797162036262872	21.595902796314512
17	23.134535309923212	24.296766835998643	27.801118193441443	24.767579660636706
18	23.407903797557754	25.39470691304452	28.58663714176213	22.6107521476356
19	24.933241795050492	26.91267060862918	26.52692191445187	21.62716568186846
20	26.118634860921702	25.718968470185487	25.745453639010265	22.416943029882543
21	25.458479045038292	27.21657493796758	26.334255294177524	20.99069072281661
22	23.186674740315517	25.28793533048817	26.054031821670687	25.47135810752563
23	22.56712951663009	27.930843588979258	25.80382487383193	23.698202020558725
24	22.950697079257125	25.164130149158236	27.937490847037243	23.94768192454739
25	22.93979142150575	26.584150651794815	26.057459314106836	24.418598612592607
26	21.95205041945237	27.11478879895472	26.89626019029853	24.036900591294376
27	21.532442254542204	30.973833691835196	24.99015894217197	22.50356511145063
28	22.127995031174603	27.15456848389546	27.446113067782296	23.27132341714764
29	23.37913363377555	26.544786420482698	26.5752183987794	23.50086154696236
30	24.060165994497318	25.75075067277522	26.730598055884748	23.45848527684272
31	25.631930934988777	25.779832426778892	24.364381914057184	24.22385472417514
32	24.882244862136908	26.40976399117992	26.127982567565745	22.58000857911743
33	24.302583186799374	25.901041022929927	24.989016444693256	24.807359345577446
34	24.71398614254422	24.743898803805138	27.27962002611126	23.26249502753938
35	24.041574444616394	24.162471450545958	27.358348488735494	24.43760561610215
36	25.18241010881769	27.057248471390306	25.547178394749498	22.21316302504251
37	24.28357618328983	27.002304729004788	26.535127123617187	22.178991964088187
38	24.771941923737252	26.664229338712076	26.51123853997131	22.05259019757936
39	25.053931074165742	25.134840668340253	26.450686173599376	23.36054208389463
40	25.29157054973863	23.76238960618112	29.366859056317857	21.579180787762397
41	21.647419046263877	25.918074621703507	28.26393353572849	24.170572796304125
42	22.48518128838402	26.167969979320794	27.832381078995393	23.514467653299793
43	22.803834221538573	24.652499005507877	27.991499818758353	24.552166954195197
44	23.91870403395087	24.42960813375114	27.530761744614423	24.120926087683568
45	24.51155636199721	23.77994252199048	26.942271679668632	24.766229436343675
46	24.526720419441983	25.171504451066312	26.958058917556343	23.34371621193536
47	23.625809225771004	25.200378478255676	28.60107415535681	22.572738140616515
48	22.748371162117277	24.608357057466584	28.68883873440361	23.95443304601253
49	23.594234749995586	24.449757634739402	27.956393987139634	23.99961362812538
50	22.411126679081804	24.18594458056321	28.97643650881852	24.42649223153646
51	22.038568637613302	23.509482209756307	27.227999912754736	27.223949239875655
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1457.0
1	1970.5
2	2484.0
3	2760.5
4	3037.0
5	2333.5
6	1630.0
7	1676.5
8	1723.0
9	1831.5
10	1940.0
11	1956.5
12	1973.0
13	2112.0
14	2251.0
15	2139.5
16	2028.0
17	2200.5
18	2373.0
19	2313.0
20	2253.0
21	2543.5
22	2834.0
23	3270.0
24	3706.0
25	4555.5
26	6331.0
27	7257.0
28	9036.5
29	10816.0
30	12765.0
31	14714.0
32	17231.0
33	19748.0
34	21004.5
35	22261.0
36	23813.5
37	25366.0
38	27108.5
39	28851.0
40	32223.5
41	35596.0
42	40564.5
43	45533.0
44	51966.5
45	58400.0
46	66395.0
47	74390.0
48	81690.5
49	88991.0
50	89243.0
51	89495.0
52	80761.0
53	72027.0
54	65453.5
55	58880.0
56	56354.5
57	53829.0
58	50693.0
59	47557.0
60	45755.5
61	43954.0
62	40662.0
63	37370.0
64	32437.0
65	27504.0
66	23418.0
67	19332.0
68	16111.5
69	12891.0
70	11945.5
71	11000.0
72	9626.0
73	8252.0
74	6769.0
75	4652.0
76	4018.0
77	3123.5
78	2229.0
79	1654.0
80	1079.0
81	862.0
82	645.0
83	436.5
84	228.0
85	164.5
86	101.0
87	73.0
88	45.0
89	34.0
90	23.0
91	18.5
92	14.0
93	16.5
94	19.0
95	12.5
96	6.0
97	3.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	962803.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.21635076806217
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.30519558084275	22.45231853560208
2	10.195911277431009	6.161664631177924
3	3.9333128773900987	3.565510847512654
4	1.9280705056885183	2.3303701882175716
5	1.1725621307272522	1.7715274319700511
6	0.7859728431465223	1.424953867361186
7	0.5696725119686069	1.2049397111196565
8	0.47084644222282807	1.1381809004879275
9	0.4038488363342488	1.0982554286354456
>10	5.388160603321592	39.295098039826605
>50	0.7785661352890668	15.039594936711692
>100	0.06273781202856127	3.1691468156793765
>500	0.003428295739265199	0.6997544124444308
>1k	0.0017141478696325995	0.6486842532534457
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTGC	1602	0.1663891782638816	TruSeq Adapter, Index 21 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTGC	1345	0.13969628262479447	TruSeq Adapter, Index 21 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1148	0.11923519141506622	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1108	0.11508065512882697	No Hit
CTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTGCT	1059	0.10999134817818391	TruSeq Adapter, Index 15 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.12307813747983752	0.0
2	0.0	0.0	0.0	0.5930600548606516	0.0
3	0.0	0.0	0.0	0.8473176755784932	0.0
4	0.0	0.0	0.0	1.1856007926855234	0.0
5	0.0	0.0	0.0	2.1528807035291746	0.0
6	0.0	0.0	0.0	3.054830531271714	0.0
7	0.0	0.0	0.0	3.770760996797891	0.0
8	0.0	0.0	0.0	5.004969864032414	0.0
9	0.0	0.0	0.0	5.614024883595087	0.0
10	0.0	0.0	0.0	6.80658452456006	0.0
11	0.0	0.0	0.0	8.101657348387988	0.0
12	0.0	0.0	0.0	9.151404804513488	0.0
13	0.0	0.0	0.0	9.566546842915944	0.0
14	0.0	0.0	0.0	9.745815083667168	0.0
15	0.0	0.0	0.0	10.027596507281345	0.0
16	0.0	0.0	0.0	10.658151252125306	0.0
17	0.0	0.0	0.0	11.337729525146889	0.0
18	1.0386340715598103E-4	0.0	0.0	12.205819882156579	0.0
19	1.0386340715598103E-4	0.0	0.0	12.620338740116098	0.0
20	1.0386340715598103E-4	0.0	0.0	13.050229382334704	0.0
21	1.0386340715598103E-4	0.0	0.0	13.527066284587812	0.0
22	1.0386340715598103E-4	0.0	0.0	14.013458620299271	0.0
23	1.0386340715598103E-4	0.0	0.0	14.509614116283393	0.0
24	1.0386340715598103E-4	0.0	0.0	14.914577540784563	0.0
25	1.0386340715598103E-4	0.0	0.0	15.267193808079119	0.0
26	1.0386340715598103E-4	0.0	0.0	15.580965161097337	0.0
27	1.0386340715598103E-4	0.0	0.0	15.90626535230987	0.0
28	1.0386340715598103E-4	0.0	0.0	16.27560362815654	0.0
29	1.0386340715598103E-4	0.0	0.0	16.64276077245293	0.0
30	1.0386340715598103E-4	0.0	0.0	17.089996603666584	0.0
31	1.0386340715598103E-4	0.0	0.0	17.485923911745186	0.0
32	1.0386340715598103E-4	0.0	0.0	17.88174735641663	0.0
33	1.0386340715598103E-4	0.0	0.0	18.279128752195412	0.0
34	1.0386340715598103E-4	0.0	0.0	18.655322012914375	0.0
35	4.154536286239241E-4	0.0	0.0	19.06246656896582	0.0
36	4.154536286239241E-4	0.0	0.0	19.4501886678791	0.0
37	5.193170357799051E-4	0.0	0.0	19.853801868087242	0.0
38	5.193170357799051E-4	0.0	0.0	20.242874191293545	0.0
39	5.193170357799051E-4	0.0	0.0	20.64981102053068	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCACG	25	3.8903323E-5	45.0	23
CGCATCG	55	1.8189894E-12	45.0	21
ATTACGA	25	3.8903323E-5	45.0	30
CATTACG	25	3.8903323E-5	45.0	29
TACGAGT	25	3.8903323E-5	45.0	32
CAATGCG	20	7.0327154E-4	45.0	1
AGTACGG	95	0.0	45.0	2
GTACGAT	45	3.8380676E-10	45.0	10
CAGACGA	20	7.0327154E-4	45.0	25
CGACCTA	30	2.165134E-6	44.999996	9
TTACGCG	30	2.165134E-6	44.999996	1
TAGGACG	30	2.165134E-6	44.999996	1
TAATGCG	30	2.165134E-6	44.999996	1
TTAACGG	75	0.0	42.0	2
GCTACGA	140	0.0	41.785713	10
TTCGTAC	60	3.6379788E-12	41.249996	33
TCGTACA	60	3.6379788E-12	41.249996	34
TACGATT	50	1.0804797E-9	40.5	11
CTACGCA	95	0.0	40.263157	15
CGAATAT	150	0.0	39.0	14
>>END_MODULE