FastQCFastQC Report
Sat 18 Jun 2016
SRR3554006_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554006_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences565641
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11570.20454670011544424No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8840.15628287199831695No Hit
GCTGTCTCTTATACACATCTGACGCACAGTTCGTCGTATGCCGTCTTCTGC6830.12074796558241004No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6770.11968722210730835No Hit
CTGTCTCTTATACACATCTGACGCACAGTTCGTCGTATGCCGTCTTCTGCT6050.10695830040608797No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG5830.10306890766404839No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACGT207.030245E-445.018
CGGCGTA253.888285E-545.041
CCTTAAC207.030245E-445.038
ACACGAT207.030245E-445.010
ACGTTAG253.888285E-545.01
CGGTTAC253.888285E-545.022
GACCCAA207.030245E-445.030
AACGGTT253.888285E-545.020
TACGCTA253.888285E-545.026
CGTACAT253.888285E-545.035
TCGTACA253.888285E-545.034
TATACGT207.030245E-445.030
TGTAACG207.030245E-445.01
GACCTAG253.888285E-545.01
ATCGGGT302.1635387E-644.9999964
AACACGT1200.044.99999641
GGTATGC1200.043.1249968
ACATACG1600.042.187517
CGTTAGG1100.040.9090922
GTACGAG556.002665E-1140.9090921