##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554006_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	565641
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.855841425922094	31.0	31.0	34.0	30.0	34.0
2	31.982414641088607	33.0	31.0	34.0	30.0	34.0
3	32.00048087037538	33.0	31.0	34.0	30.0	34.0
4	35.63282011028196	37.0	35.0	37.0	33.0	37.0
5	35.46552848891788	37.0	35.0	37.0	33.0	37.0
6	35.41429811488205	37.0	35.0	37.0	33.0	37.0
7	35.709209551641415	37.0	35.0	37.0	35.0	37.0
8	35.58353089680557	37.0	35.0	37.0	35.0	37.0
9	37.35396125811248	39.0	37.0	39.0	34.0	39.0
10	36.812695685072335	39.0	37.0	39.0	32.0	39.0
11	36.7106203404633	39.0	37.0	39.0	32.0	39.0
12	36.589151069317815	39.0	35.0	39.0	32.0	39.0
13	36.41670953838212	39.0	35.0	39.0	32.0	39.0
14	37.51076389441359	39.0	36.0	41.0	32.0	41.0
15	37.680905379914115	39.0	36.0	41.0	33.0	41.0
16	37.72444006003808	40.0	36.0	41.0	33.0	41.0
17	37.6667020247825	39.0	36.0	41.0	33.0	41.0
18	37.65343742762636	39.0	36.0	41.0	33.0	41.0
19	37.59065378924088	39.0	36.0	41.0	33.0	41.0
20	37.50446484607728	39.0	36.0	41.0	32.0	41.0
21	37.36448736919707	39.0	35.0	41.0	32.0	41.0
22	37.36353270006948	39.0	35.0	41.0	32.0	41.0
23	36.85585556916843	39.0	35.0	41.0	31.0	41.0
24	36.96552230124761	39.0	35.0	41.0	31.0	41.0
25	36.94863172931241	39.0	35.0	41.0	31.0	41.0
26	36.93849632540781	39.0	35.0	41.0	31.0	41.0
27	34.56198189310888	39.0	35.0	40.0	24.0	41.0
28	35.41131035409385	38.0	35.0	40.0	23.0	41.0
29	36.11496691364311	38.0	35.0	40.0	29.0	41.0
30	36.40572553969744	38.0	35.0	40.0	30.0	41.0
31	36.48106838082812	38.0	35.0	40.0	30.0	41.0
32	36.242965060877836	38.0	35.0	40.0	30.0	41.0
33	36.262201643798804	38.0	35.0	40.0	30.0	41.0
34	36.17325830341153	38.0	35.0	40.0	30.0	41.0
35	36.10490752968756	38.0	35.0	40.0	30.0	41.0
36	36.00323526759906	38.0	35.0	40.0	29.0	41.0
37	35.97381377941132	38.0	35.0	40.0	29.0	41.0
38	35.856324064203264	38.0	35.0	40.0	29.0	41.0
39	35.756168665284164	38.0	35.0	40.0	29.0	41.0
40	35.58902908381818	38.0	34.0	40.0	28.0	41.0
41	35.476584971740024	38.0	34.0	40.0	27.0	41.0
42	35.38941130505037	38.0	34.0	40.0	27.0	41.0
43	35.29944788302121	38.0	34.0	40.0	27.0	41.0
44	35.12360313343623	38.0	34.0	40.0	26.0	41.0
45	35.07134560613534	37.0	34.0	40.0	26.0	41.0
46	34.898997774206606	37.0	34.0	40.0	26.0	41.0
47	34.81849795188114	37.0	34.0	40.0	26.0	41.0
48	34.734755790333445	37.0	33.0	40.0	26.0	41.0
49	34.67219667598353	37.0	33.0	40.0	26.0	41.0
50	34.536829897408424	36.0	33.0	40.0	26.0	41.0
51	32.965610696537205	35.0	31.0	39.0	21.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	1.0
11	2.0
12	3.0
13	6.0
14	18.0
15	34.0
16	74.0
17	168.0
18	319.0
19	572.0
20	917.0
21	1434.0
22	2106.0
23	2912.0
24	3846.0
25	4921.0
26	6006.0
27	7310.0
28	8867.0
29	10729.0
30	13163.0
31	16743.0
32	21648.0
33	28525.0
34	43414.0
35	52299.0
36	52956.0
37	71220.0
38	99804.0
39	115596.0
40	25.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.31970277967827	17.974828557335837	23.758001983590297	14.947466679395587
2	29.181936952943648	25.74123870087211	27.03958871439659	18.037235631787656
3	32.7531066524527	24.800536029036085	26.353641267164157	16.092716051347054
4	29.028305939633086	27.196225167553273	25.141388265702098	18.634080627111544
5	24.129262199875892	31.94322900921256	24.084003811604887	19.843504979306662
6	24.28837372114115	38.362141358211304	23.310014655939014	14.039470264708534
7	81.85722039243973	6.8898824519439	7.146405582339328	4.106491573277044
8	83.94936010649864	5.749406425630391	6.083363829708242	4.217869638162722
9	78.17591016209929	8.218110073350411	8.45607019293156	5.149909571618748
10	41.47524666705561	26.490300384873088	15.599116754266399	16.435336193804908
11	32.21601687289288	27.36382263661934	21.588427995848956	18.831732494638825
12	29.15435762259101	23.928074520764937	27.403070145198104	19.51449771144595
13	25.30191411160082	25.830517943359833	29.05606206056492	19.81150588447443
14	21.571632890826514	28.499702107874075	27.65040016547598	22.27826483582343
15	21.5102865598498	26.096057393293627	31.79454813211914	20.599107914737438
16	23.42174630198306	24.60023230282105	30.337440178487768	21.640581216708124
17	23.057734499443995	24.086302089134275	27.383446390908723	25.472517020513013
18	23.881932179598014	25.19177358076943	27.97887706159914	22.947417178033415
19	25.33462036874979	26.919371120551727	25.138382825855977	22.607625684842507
20	27.008827153618636	25.609176138221944	25.35212263608897	22.029874072070445
21	25.9226258351145	27.706796360235558	25.2050328742082	21.165544930441747
22	23.965554123551865	25.83334659262677	24.708781718439788	25.49231756538158
23	23.703373694622563	28.665531671148308	24.804602212357306	22.826492421871826
24	23.527643858914047	26.59831942875428	26.620771832310602	23.253264880021074
25	23.64078982959156	27.257394707950805	25.25329670232533	23.848518760132308
26	22.088568544359408	27.94175103997058	26.053981235447925	23.915699180222084
27	22.289225851732812	32.07705947765456	24.318428119602363	21.31528655101027
28	21.990096191754134	28.562285972905077	27.02014175068639	22.4274760846544
29	22.93044528243179	27.27913994919039	25.88443907001084	23.905975698366984
30	23.73413525540051	27.07494683023331	26.05327407313119	23.13764384123499
31	25.760685664582304	27.692653113900867	23.407779846227555	23.138881375289273
32	25.528382843535034	28.306646795405566	23.72211349601602	22.442856865043375
33	24.816093600004244	27.508967702129087	22.920014638259957	24.75492405960671
34	22.804216808894687	28.079294110575436	25.774828910916998	23.341660169612883
35	24.41601651931172	26.439561488647396	24.713024692340195	24.431397299700695
36	24.755454431344262	28.637598759637296	24.25230844298769	22.354638366030752
37	24.19803373517832	28.433582431259403	25.229076392977174	22.139307440585107
38	24.03132021900817	28.025903355661985	24.923405481568697	23.01937094376115
39	25.761392826899037	24.899892334537277	26.239257762432356	23.099457076131326
40	24.287843349403598	25.65337378301785	27.46653796312502	22.592244904453533
41	22.047729920567992	26.499316704411456	26.390413707634348	25.0625396673862
42	23.75287505679397	26.579933208519186	25.369801694007332	24.297390040679513
43	25.022054624753153	24.737068211109168	26.133183414922183	24.107693749215493
44	23.606315666650758	26.273201553635612	26.268074626839283	23.85240815287435
45	23.7774489473005	25.7240900146913	25.847313048382276	24.65114798962593
46	23.175123444021914	27.465830800808288	26.03258957536671	23.32645617980309
47	23.49511439234426	25.924924112643886	27.225395613118568	23.354565881893286
48	23.02449787055747	25.404806228685683	26.662494408997933	24.90820149175891
49	23.722290286595207	25.683074600320698	27.055323075943928	23.539312037140164
50	22.42075804264542	25.649484390275813	27.576678493956415	24.353079073122352
51	23.028740844457882	25.34575817523836	25.474108135725665	26.151392844578098
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1357.0
1	1688.5
2	2020.0
3	2214.5
4	2409.0
5	1807.5
6	1206.0
7	1262.5
8	1319.0
9	1362.5
10	1406.0
11	1479.0
12	1552.0
13	1597.5
14	1643.0
15	1565.0
16	1487.0
17	1636.5
18	1786.0
19	1708.5
20	1631.0
21	1752.5
22	1874.0
23	2159.5
24	2445.0
25	2835.0
26	4261.5
27	5298.0
28	6403.0
29	7508.0
30	8404.5
31	9301.0
32	10260.5
33	11220.0
34	12688.0
35	14156.0
36	14926.0
37	15696.0
38	16425.5
39	17155.0
40	19672.0
41	22189.0
42	24620.5
43	27052.0
44	30589.0
45	34126.0
46	37037.5
47	39949.0
48	42726.0
49	45503.0
50	45360.5
51	45218.0
52	41508.0
53	37798.0
54	35735.5
55	33673.0
56	31901.0
57	30129.0
58	28508.5
59	26888.0
60	26047.0
61	25206.0
62	23208.0
63	21210.0
64	19018.0
65	16826.0
66	15270.0
67	13714.0
68	12350.5
69	10987.0
70	10111.5
71	9236.0
72	8222.0
73	7208.0
74	6461.0
75	4387.5
76	3061.0
77	2474.5
78	1888.0
79	1534.0
80	1180.0
81	933.0
82	686.0
83	506.0
84	326.0
85	230.5
86	135.0
87	82.5
88	30.0
89	20.0
90	10.0
91	6.5
92	3.0
93	2.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	565641.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.258708641790143
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.43950052162415	23.129605750077907
2	8.738494233404309	5.288311019530885
3	3.3356027280293943	3.027930932766054
4	1.6823352184171474	2.036211648476274
5	1.0401381040260318	1.573661791847385
6	0.7285742697386357	1.3227429931155836
7	0.5604368701985516	1.1870667177218313
8	0.4713491790914839	1.1409933982940939
9	0.40073733377566756	1.091321480214516
>10	5.799322595533024	42.22301318362853
>50	0.7486323466754281	14.450676529201534
>100	0.05083306057672659	2.6328459698743596
>500	0.003465890493867722	0.6933877688701842
>1k	5.776484156446203E-4	0.20223081638084273
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1157	0.20454670011544424	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	884	0.15628287199831695	No Hit
GCTGTCTCTTATACACATCTGACGCACAGTTCGTCGTATGCCGTCTTCTGC	683	0.12074796558241004	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	677	0.11968722210730835	No Hit
CTGTCTCTTATACACATCTGACGCACAGTTCGTCGTATGCCGTCTTCTGCT	605	0.10695830040608797	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	583	0.10306890766404839	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.7679057918361645E-4	0.0	0.0	0.11402992357343261	0.0
2	1.7679057918361645E-4	0.0	0.0	0.44515867838434625	0.0
3	1.7679057918361645E-4	0.0	0.0	0.5793427279847112	0.0
4	1.7679057918361645E-4	0.0	0.0	0.7826518940458701	0.0
5	1.7679057918361645E-4	0.0	0.0	1.3543926271256856	0.0
6	1.7679057918361645E-4	0.0	0.0	1.8856483175724532	0.0
7	1.7679057918361645E-4	0.0	0.0	2.3506075408253646	0.0
8	1.7679057918361645E-4	0.0	0.0	3.304039134362608	0.0
9	1.7679057918361645E-4	0.0	0.0	3.755739064176748	0.0
10	1.7679057918361645E-4	0.0	0.0	4.758141648147854	0.0
11	1.7679057918361645E-4	0.0	0.0	5.7734499443993625	0.0
12	1.7679057918361645E-4	0.0	0.0	6.5852722840105296	0.0
13	1.7679057918361645E-4	0.0	0.0	6.914633133029607	0.0
14	1.7679057918361645E-4	0.0	0.0	7.046342114521401	0.0
15	1.7679057918361645E-4	0.0	0.0	7.2641481080756165	0.0
16	1.7679057918361645E-4	0.0	0.0	7.711958645147718	0.0
17	1.7679057918361645E-4	0.0	0.0	8.194420135739806	0.0
18	1.7679057918361645E-4	0.0	0.0	8.838645006284905	0.0
19	1.7679057918361645E-4	0.0	0.0	9.107897058381553	0.0
20	3.535811583672329E-4	0.0	0.0	9.38934766044187	0.0
21	3.535811583672329E-4	0.0	0.0	9.693250666058507	0.0
22	3.535811583672329E-4	0.0	0.0	10.006523572371876	0.0
23	3.535811583672329E-4	0.0	0.0	10.351972364096662	0.0
24	3.535811583672329E-4	0.0	0.0	10.624053065460247	0.0
25	7.071623167344658E-4	0.0	0.0	10.861129232145478	0.0
26	7.071623167344658E-4	0.0	0.0	11.082824618441732	0.0
27	7.071623167344658E-4	0.0	0.0	11.314243486593087	0.0
28	7.071623167344658E-4	0.0	0.0	11.56175029745015	0.0
29	7.071623167344658E-4	0.0	0.0	11.815798359737006	0.0
30	7.071623167344658E-4	0.0	0.0	12.14056265369731	0.0
31	7.071623167344658E-4	0.0	0.0	12.412643355060895	0.0
32	7.071623167344658E-4	0.0	0.0	12.688259868008153	0.0
33	7.071623167344658E-4	0.0	0.0	12.966528239643166	0.0
34	7.071623167344658E-4	0.0	0.0	13.243028705486342	0.0
35	7.071623167344658E-4	0.0	0.0	13.545340595890327	0.0
36	7.071623167344658E-4	0.0	0.0	13.81830525014983	0.0
37	7.071623167344658E-4	0.0	0.0	14.08914841745913	0.0
38	7.071623167344658E-4	0.0	0.0	14.363704186931287	0.0
39	7.071623167344658E-4	0.0	0.0	14.644094045516503	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAACGT	20	7.030245E-4	45.0	18
CGGCGTA	25	3.888285E-5	45.0	41
CCTTAAC	20	7.030245E-4	45.0	38
ACACGAT	20	7.030245E-4	45.0	10
ACGTTAG	25	3.888285E-5	45.0	1
CGGTTAC	25	3.888285E-5	45.0	22
GACCCAA	20	7.030245E-4	45.0	30
AACGGTT	25	3.888285E-5	45.0	20
TACGCTA	25	3.888285E-5	45.0	26
CGTACAT	25	3.888285E-5	45.0	35
TCGTACA	25	3.888285E-5	45.0	34
TATACGT	20	7.030245E-4	45.0	30
TGTAACG	20	7.030245E-4	45.0	1
GACCTAG	25	3.888285E-5	45.0	1
ATCGGGT	30	2.1635387E-6	44.999996	4
AACACGT	120	0.0	44.999996	41
GGTATGC	120	0.0	43.124996	8
ACATACG	160	0.0	42.1875	17
CGTTAGG	110	0.0	40.909092	2
GTACGAG	55	6.002665E-11	40.909092	1
>>END_MODULE