FastQCFastQC Report
Sat 18 Jun 2016
SRR3554001_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554001_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences686627
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10090.1469502364456976No Hit
GCTAAAGGTGTAATTTGAAATGGCCTTCGGGTAAATGCAAGATACTTAACT8900.12961913819293444No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8390.12219152465603597No Hit
GCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTCTGC8110.11811361918479756No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA7120.10369531055434757No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG7050.10267583418653796No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCT207.0313E-445.030
ACCGCAT207.0313E-445.015
ATTCACG207.0313E-445.034
TATTCGA253.8891594E-545.026
CATACGT207.0313E-445.014
CAGTCGA253.8891594E-545.039
AATAGTC207.0313E-445.034
ATACGTC207.0313E-445.015
CGTCCAA207.0313E-445.018
TATCGTC207.0313E-445.026
ATCGCCC253.8891594E-545.029
TCTATCG207.0313E-445.024
CTAACGG302.1642209E-644.9999962
ATACGAG302.1642209E-644.9999961
TGCGTAG600.044.9999961
CGAATAT1050.040.71428714
TGCATAG501.0804797E-940.51
GTTGATC1400.040.17857416
CGTAAGG1100.038.8636362
GACGTGA356.2446125E-638.571439