##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554001_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	686627
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.87600108938332	31.0	31.0	34.0	30.0	34.0
2	31.98794542014806	33.0	31.0	34.0	30.0	34.0
3	32.01316872188248	33.0	31.0	34.0	30.0	34.0
4	35.63935877849254	37.0	35.0	37.0	33.0	37.0
5	35.47726494880044	37.0	35.0	37.0	33.0	37.0
6	35.41726148258079	37.0	35.0	37.0	33.0	37.0
7	35.70440428354842	37.0	35.0	37.0	35.0	37.0
8	35.57471960758898	37.0	35.0	37.0	35.0	37.0
9	37.33255173478468	39.0	37.0	39.0	34.0	39.0
10	36.75419259656262	39.0	37.0	39.0	32.0	39.0
11	36.67342676591512	39.0	35.0	39.0	32.0	39.0
12	36.58960104976938	39.0	35.0	39.0	32.0	39.0
13	36.48434011479304	39.0	35.0	39.0	32.0	39.0
14	37.58261618025507	39.0	36.0	41.0	32.0	41.0
15	37.72553220307387	40.0	36.0	41.0	33.0	41.0
16	37.77808184065002	40.0	36.0	41.0	33.0	41.0
17	37.71701811900785	40.0	36.0	41.0	33.0	41.0
18	37.690992343732475	39.0	36.0	41.0	33.0	41.0
19	37.65096187595303	39.0	36.0	41.0	33.0	41.0
20	37.544199397926384	39.0	36.0	41.0	32.0	41.0
21	37.44790257301271	39.0	36.0	41.0	32.0	41.0
22	37.425239613356304	39.0	36.0	41.0	32.0	41.0
23	36.93267086788023	39.0	35.0	41.0	31.0	41.0
24	37.014654244589856	39.0	35.0	41.0	31.0	41.0
25	37.01953316720723	39.0	35.0	41.0	31.0	41.0
26	37.0251155285184	39.0	35.0	41.0	31.0	41.0
27	34.64052389434147	39.0	35.0	41.0	24.0	41.0
28	35.48685967781634	39.0	35.0	40.0	23.0	41.0
29	36.1894157963494	39.0	35.0	40.0	29.0	41.0
30	36.46096060888954	38.0	35.0	40.0	30.0	41.0
31	36.544551845470686	39.0	35.0	40.0	30.0	41.0
32	36.33533053608436	39.0	35.0	40.0	30.0	41.0
33	36.36202625297287	39.0	35.0	40.0	30.0	41.0
34	36.303648123362464	39.0	35.0	40.0	30.0	41.0
35	36.23174882432529	38.0	35.0	40.0	30.0	41.0
36	36.10135779688244	38.0	35.0	40.0	29.0	41.0
37	36.00240887701765	38.0	35.0	40.0	29.0	41.0
38	35.879395945688124	38.0	35.0	40.0	29.0	41.0
39	35.753650817692865	38.0	34.0	40.0	28.0	41.0
40	35.56606570962109	38.0	34.0	40.0	28.0	41.0
41	35.42158988795955	38.0	34.0	40.0	27.0	41.0
42	35.34346595749949	38.0	34.0	40.0	27.0	41.0
43	35.186536503807744	37.0	34.0	40.0	27.0	41.0
44	35.052728774137925	37.0	34.0	40.0	26.0	41.0
45	34.95731743726943	37.0	33.0	40.0	26.0	41.0
46	34.762550846383846	37.0	33.0	40.0	26.0	41.0
47	34.603039204691925	36.0	33.0	40.0	26.0	41.0
48	34.50652828974101	36.0	33.0	40.0	26.0	41.0
49	34.40246451129944	36.0	33.0	40.0	26.0	41.0
50	34.262052031161026	36.0	33.0	40.0	25.0	41.0
51	32.702425043000055	35.0	31.0	39.0	21.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	4.0
10	4.0
11	8.0
12	5.0
13	5.0
14	30.0
15	42.0
16	105.0
17	195.0
18	348.0
19	656.0
20	1033.0
21	1563.0
22	2286.0
23	3198.0
24	4220.0
25	5618.0
26	7275.0
27	9032.0
28	10953.0
29	13423.0
30	16561.0
31	20568.0
32	26296.0
33	35631.0
34	52485.0
35	62374.0
36	67073.0
37	90198.0
38	117824.0
39	137586.0
40	25.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.39233091620341	17.376246491908997	21.717322505523377	15.514100086364213
2	29.175520333456156	25.8020730323742	26.486869872580016	18.535536761589626
3	33.54528732485032	24.801966715552986	25.541378361177173	16.11136759841952
4	29.551124555253434	27.218999544148424	24.590352549491936	18.639523351106206
5	25.586380960841915	31.624448208415924	23.511018354943804	19.27815247579836
6	25.172764834473448	37.57498612783942	22.497367566378834	14.754881471308293
7	83.11412164100742	6.5079002136531185	6.603876631708337	3.774101513631127
8	84.64610334286301	5.367834355479759	6.055252706345658	3.9308095953115743
9	78.46501812483343	7.6642776937114325	8.444177115085774	5.426527066369368
10	40.703904740128195	26.925972908143724	15.707509317285803	16.66261303444228
11	31.25204805520319	27.348036124416897	21.877380295269482	19.52253552511043
12	27.16788008627683	24.34116339730305	28.069388474382745	20.42156804203738
13	24.776479806357745	25.831929126002912	29.14187761331844	20.249713454320904
14	22.060157844069632	28.254787533842972	27.38677622639366	22.298278395693732
15	22.08972265873611	26.80072295438426	30.134410677121636	20.97514370975799
16	24.444858707857396	24.37466047795965	29.05099857710227	22.129482237080687
17	23.928275468340164	24.94367393067852	26.4048748447119	24.723175756269416
18	25.105916312641362	25.541960919101637	26.7918389460362	22.560283822220796
19	26.439828320179657	26.626392495488815	24.651375492079396	22.282403692252124
20	26.43138123027495	26.053155497817592	25.04096110406378	22.474502167843678
21	26.949420864603347	26.488180627910058	25.40272957515507	21.159668932331527
22	24.972219268977188	25.345930177519964	24.28276196537567	25.399088588127178
23	24.32732764659706	27.802140026535515	24.726234185372846	23.14429814149458
24	23.163231274039617	26.71494130000714	27.010735086153037	23.111092339800212
25	24.509959555916094	26.860143862679447	25.18616366673609	23.443732914668374
26	23.294306807043707	26.939371740406365	25.164172105087623	24.602149347462305
27	23.40892507868173	30.775078754549412	24.525979898838816	21.29001626793004
28	23.29255913327032	27.239389071504615	26.616197731810722	22.851854063414343
29	24.653997002739477	26.12757727266769	25.23713748512657	23.981288239466263
30	25.646530066542677	26.18393975185945	25.515745812500818	22.65378436909705
31	26.62522737963989	27.070592912891573	22.98176448056951	23.32241522689903
32	26.190347889028544	27.528337802038077	23.60408198337671	22.67723232555667
33	24.917313184596587	27.382989599884656	23.108907747583476	24.590789467935284
34	23.64995841992814	28.695783882661186	24.429566562340252	23.224691135070426
35	26.037426433857103	26.644597430628274	23.84759119580209	23.470384939712538
36	25.452101359253277	29.331208938768793	23.209690268515512	22.00699943346242
37	24.890806799033534	29.17508341501281	23.864776654573735	22.06933313137992
38	23.275664953460904	30.309469333422655	23.929440584189088	22.48542512892735
39	25.162570070795354	27.83243303860757	24.03255333681897	22.972443553778106
40	24.980520719400783	27.783498172952708	25.976840409712988	21.25914069793352
41	22.66878523565196	28.33445233001324	25.437683050622827	23.55907938371197
42	23.90249728018269	27.814810661392574	24.67700804075575	23.60568401766898
43	24.32965787829491	25.376223189592018	26.139956628562523	24.154162303550546
44	24.035174847479055	26.295062675950696	25.633568152723385	24.036194323846864
45	25.154414259852874	25.7062422538001	24.68356181740596	24.45578166894107
46	24.187950663169378	26.910826402107695	25.433313866189355	23.467909068533572
47	23.957985922487758	26.99981212506936	25.789111118554906	23.253090833887978
48	23.280325416856602	27.6141194564152	25.954120650658947	23.151434476069248
49	23.395089327975743	27.05515512789331	26.485267838287747	23.064487705843202
50	22.648250068814654	26.429196638058215	27.05923303336455	23.86332025976258
51	23.1565318579083	25.873582017601986	26.052136021449783	24.917750103039932
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1389.0
1	1682.5
2	1976.0
3	2157.0
4	2338.0
5	1742.5
6	1147.0
7	1187.5
8	1228.0
9	1306.5
10	1385.0
11	1412.0
12	1439.0
13	1477.0
14	1515.0
15	1455.0
16	1395.0
17	1415.5
18	1436.0
19	1354.5
20	1273.0
21	1530.0
22	1787.0
23	2078.0
24	2369.0
25	3188.0
26	5119.0
27	6231.0
28	7231.0
29	8231.0
30	9528.0
31	10825.0
32	12104.0
33	13383.0
34	15448.0
35	17513.0
36	18366.0
37	19219.0
38	20856.0
39	22493.0
40	23956.0
41	25419.0
42	28745.0
43	32071.0
44	36381.5
45	40692.0
46	43758.5
47	46825.0
48	50644.0
49	54463.0
50	54752.0
51	55041.0
52	51306.0
53	47571.0
54	44862.5
55	42154.0
56	40785.0
57	39416.0
58	36975.0
59	34534.0
60	33889.5
61	33245.0
62	31103.0
63	28961.0
64	25788.5
65	22616.0
66	20431.5
67	18247.0
68	15491.0
69	12735.0
70	11470.0
71	10205.0
72	8635.5
73	7066.0
74	6289.5
75	4431.5
76	3350.0
77	2619.5
78	1889.0
79	1461.0
80	1033.0
81	817.0
82	601.0
83	421.0
84	241.0
85	157.5
86	74.0
87	67.0
88	60.0
89	34.0
90	8.0
91	8.0
92	8.0
93	5.5
94	3.0
95	4.5
96	6.0
97	3.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	686627.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.320389271014168
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.00423760349874	22.28473832781236
2	8.535044718375772	5.005016671765823
3	3.166582304015745	2.785362774373397
4	1.6507173110458864	1.9359869654506867
5	1.0465067058981392	1.5341991995830089
6	0.7939340272616313	1.3967072840889
7	0.6183333869819695	1.2690842923903192
8	0.5172645289989696	1.2133117877070068
9	0.44267956069039116	1.1681583337587431
>10	6.488537332671274	45.33987921223451
>50	0.6841403691086031	12.558623283089384
>100	0.04711440131583124	2.436898141491761
>500	0.004416975123359179	0.926841507490312
>1k	4.907750137065754E-4	0.14519221876381383
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1009	0.1469502364456976	No Hit
GCTAAAGGTGTAATTTGAAATGGCCTTCGGGTAAATGCAAGATACTTAACT	890	0.12961913819293444	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	839	0.12219152465603597	No Hit
GCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTCTGC	811	0.11811361918479756	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	712	0.10369531055434757	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	705	0.10267583418653796	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.09204415206509502	0.0
2	0.0	0.0	0.0	0.39861525981355234	0.0
3	0.0	0.0	0.0	0.5372640458356575	0.0
4	0.0	0.0	0.0	0.7346055427473723	0.0
5	0.0	0.0	0.0	1.2755105756109213	0.0
6	0.0	0.0	0.0	1.785540038477951	0.0
7	0.0	0.0	0.0	2.200758199138688	0.0
8	0.0	0.0	0.0	2.9484712951864696	0.0
9	0.0	0.0	0.0	3.296549655052889	0.0
10	0.0	0.0	0.0	3.974938358089618	0.0
11	0.0	0.0	0.0	4.728185754419794	0.0
12	0.0	0.0	0.0	5.340454133030015	0.0
13	2.9127896223131336E-4	0.0	0.0	5.5756618950318	0.0
14	2.9127896223131336E-4	0.0	0.0	5.666686570729086	0.0
15	2.9127896223131336E-4	0.0	0.0	5.822229536560607	0.0
16	2.9127896223131336E-4	0.0	0.0	6.195357887178919	0.0
17	4.3691844334697004E-4	0.0	0.0	6.619605695668827	0.0
18	4.3691844334697004E-4	0.0	0.0	7.15468514928775	0.0
19	4.3691844334697004E-4	0.0	0.0	7.375620242140202	0.0
20	4.3691844334697004E-4	0.0	0.0	7.614032072726531	0.0
21	4.3691844334697004E-4	0.0	0.0	7.89875725830764	0.0
22	4.3691844334697004E-4	0.0	0.0	8.175180993465156	0.0
23	5.825579244626267E-4	0.0	0.0	8.47985878795911	0.0
24	5.825579244626267E-4	0.0	0.0	8.731086892883617	0.0
25	5.825579244626267E-4	0.0	0.0	8.951876346254954	0.0
26	5.825579244626267E-4	0.0	0.0	9.151548074864518	0.0
27	5.825579244626267E-4	0.0	0.0	9.358793056492098	0.0
28	5.825579244626267E-4	0.0	0.0	9.589049076135952	0.0
29	5.825579244626267E-4	0.0	0.0	9.846830957710663	0.0
30	5.825579244626267E-4	0.0	0.0	10.123982890273759	0.0
31	5.825579244626267E-4	0.0	0.0	10.378123784820579	0.0
32	7.281974055782834E-4	0.0	0.0	10.627312937009467	0.0
33	8.738368866939401E-4	0.0	0.0	10.886696852876453	0.0
34	8.738368866939401E-4	0.0	0.0	11.12452612553832	0.0
35	8.738368866939401E-4	0.0	0.0	11.404153929280382	0.0
36	8.738368866939401E-4	0.0	0.0	11.663246566185133	0.0
37	8.738368866939401E-4	0.0	0.0	11.921611005684309	0.0
38	8.738368866939401E-4	0.0	0.0	12.181140561032409	0.0
39	8.738368866939401E-4	0.0	0.0	12.428436399966794	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATCT	20	7.0313E-4	45.0	30
ACCGCAT	20	7.0313E-4	45.0	15
ATTCACG	20	7.0313E-4	45.0	34
TATTCGA	25	3.8891594E-5	45.0	26
CATACGT	20	7.0313E-4	45.0	14
CAGTCGA	25	3.8891594E-5	45.0	39
AATAGTC	20	7.0313E-4	45.0	34
ATACGTC	20	7.0313E-4	45.0	15
CGTCCAA	20	7.0313E-4	45.0	18
TATCGTC	20	7.0313E-4	45.0	26
ATCGCCC	25	3.8891594E-5	45.0	29
TCTATCG	20	7.0313E-4	45.0	24
CTAACGG	30	2.1642209E-6	44.999996	2
ATACGAG	30	2.1642209E-6	44.999996	1
TGCGTAG	60	0.0	44.999996	1
CGAATAT	105	0.0	40.714287	14
TGCATAG	50	1.0804797E-9	40.5	1
GTTGATC	140	0.0	40.178574	16
CGTAAGG	110	0.0	38.863636	2
GACGTGA	35	6.2446125E-6	38.57143	9
>>END_MODULE