SRR3554000_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3554000_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	626599
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCTGC	1037	0.16549659351515084	TruSeq Adapter, Index 13 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	952	0.15193129896472862	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	708	0.11299092401998727	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	701	0.11187378211583485	No Hit
CTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCTGCT	657	0.10485174728973394	TruSeq Adapter, Index 16 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCTGC	645	0.1029366468826155	TruSeq Adapter, Index 13 (95% over 21bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAAATT	30	2.1639153E-6	45.000004	43
TAACGCG	30	2.1639153E-6	45.000004	26
CTAACGC	30	2.1639153E-6	45.000004	25
GAATGCG	20	7.0308277E-4	45.0	1
ACTCTAC	20	7.0308277E-4	45.0	11
TACGATA	20	7.0308277E-4	45.0	34
ACGTGTA	20	7.0308277E-4	45.0	22
TACCTTA	20	7.0308277E-4	45.0	14
CGATCAA	20	7.0308277E-4	45.0	45
TCGACGT	20	7.0308277E-4	45.0	25
GCGTAAT	20	7.0308277E-4	45.0	8
TATCACC	25	3.8887683E-5	44.999996	18
TAGACCG	25	3.8887683E-5	44.999996	13
TAATGCG	25	3.8887683E-5	44.999996	1
CGAATAT	105	0.0	42.857147	14
TATCGAG	55	6.002665E-11	40.909092	1
TCGCACA	40	3.4548611E-7	39.375	34
CGCGATT	40	3.4548611E-7	39.375	22
TTATCCG	40	3.4548611E-7	39.375	1
CCGTATG	40	3.4548611E-7	39.375	1