##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554000_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	626599
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.830944511561622	31.0	31.0	34.0	30.0	34.0
2	31.95082341337921	33.0	31.0	34.0	30.0	34.0
3	31.97221668084373	33.0	31.0	34.0	30.0	34.0
4	35.60588989130209	37.0	35.0	37.0	33.0	37.0
5	35.44576994217993	37.0	35.0	37.0	33.0	37.0
6	35.392820607757116	37.0	35.0	37.0	33.0	37.0
7	35.691913009755844	37.0	35.0	37.0	35.0	37.0
8	35.545554652975824	37.0	35.0	37.0	35.0	37.0
9	37.2891578186368	39.0	37.0	39.0	34.0	39.0
10	36.723801027451366	39.0	37.0	39.0	32.0	39.0
11	36.64347852454281	39.0	35.0	39.0	32.0	39.0
12	36.539951388367996	39.0	35.0	39.0	32.0	39.0
13	36.45030075055977	39.0	35.0	39.0	32.0	39.0
14	37.524287462954774	39.0	36.0	41.0	32.0	41.0
15	37.648101896109	39.0	36.0	41.0	33.0	41.0
16	37.68181085510829	40.0	36.0	41.0	33.0	41.0
17	37.59814809790632	39.0	36.0	41.0	33.0	41.0
18	37.60285445715681	39.0	36.0	41.0	33.0	41.0
19	37.5394406949261	39.0	36.0	41.0	32.0	41.0
20	37.46179294891949	39.0	36.0	41.0	32.0	41.0
21	37.362546062154586	39.0	35.0	41.0	32.0	41.0
22	37.32266409617634	39.0	35.0	41.0	32.0	41.0
23	36.865253535355144	39.0	35.0	41.0	31.0	41.0
24	36.96835136985536	39.0	35.0	41.0	31.0	41.0
25	36.967716194887004	39.0	35.0	41.0	31.0	41.0
26	36.95861627611918	39.0	35.0	41.0	31.0	41.0
27	34.68896056329486	39.0	35.0	41.0	24.0	41.0
28	35.50404963940255	39.0	35.0	40.0	24.0	41.0
29	36.15317292239534	38.0	35.0	40.0	29.0	41.0
30	36.41505332756675	38.0	35.0	40.0	30.0	41.0
31	36.4725047438633	38.0	35.0	40.0	30.0	41.0
32	36.263280024385615	38.0	35.0	40.0	30.0	41.0
33	36.291088878213976	38.0	35.0	40.0	30.0	41.0
34	36.242515548221434	38.0	35.0	40.0	30.0	41.0
35	36.19132970209017	38.0	35.0	40.0	30.0	41.0
36	36.057816881290904	38.0	35.0	40.0	29.0	41.0
37	35.99086018330703	38.0	35.0	40.0	29.0	41.0
38	35.91737139701787	38.0	35.0	40.0	29.0	41.0
39	35.85751333787638	38.0	35.0	40.0	29.0	41.0
40	35.730452809532096	38.0	34.0	40.0	28.0	41.0
41	35.63672779560772	38.0	34.0	40.0	28.0	41.0
42	35.55039506925482	38.0	34.0	40.0	28.0	41.0
43	35.42467830302953	38.0	34.0	40.0	27.0	41.0
44	35.28841412131204	38.0	34.0	40.0	27.0	41.0
45	35.21777723871248	38.0	34.0	40.0	27.0	41.0
46	35.046667805087466	37.0	34.0	40.0	26.0	41.0
47	34.90350926190435	37.0	34.0	40.0	26.0	41.0
48	34.83037955694152	37.0	33.0	40.0	26.0	41.0
49	34.75197215444008	37.0	33.0	40.0	26.0	41.0
50	34.64892538928405	37.0	33.0	40.0	26.0	41.0
51	32.99933769444254	35.0	31.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	3.0
11	5.0
12	6.0
13	11.0
14	23.0
15	39.0
16	82.0
17	167.0
18	305.0
19	573.0
20	948.0
21	1483.0
22	2091.0
23	2927.0
24	3931.0
25	5032.0
26	6458.0
27	8037.0
28	9750.0
29	12160.0
30	15227.0
31	19245.0
32	24100.0
33	31844.0
34	48306.0
35	55921.0
36	59401.0
37	79379.0
38	109327.0
39	129783.0
40	33.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.60492116967949	17.160576381385862	23.827679265367486	15.40682318356716
2	28.322260329173844	26.078241427132827	26.97881739358026	18.620680850113068
3	32.58319914331175	25.36598366738536	25.87667710928361	16.174140080019278
4	28.961903865151395	27.129312367239656	25.03802272266633	18.87076104494262
5	24.740224609359416	31.18964441373191	24.170641829942277	19.899489146966403
6	25.05685454333633	36.40749506462666	23.77006666145334	14.765583730583675
7	81.89144891709051	6.374890480195468	7.352708829730019	4.380951772983998
8	83.44188228835347	5.714978798242576	6.366112936662842	4.477025976741106
9	76.87596054254794	7.727908917824637	9.142529751882783	6.2536007877446345
10	41.02815357190165	26.219958857259584	15.971937395367691	16.779950175471075
11	31.266248430016645	26.726183731541227	22.198726777412666	19.80884106102946
12	26.759538396965205	23.613985978273185	29.246136683907892	20.380338940853722
13	24.84060778903254	24.640160613087478	29.87811981825697	20.64111177962301
14	21.732719011680516	27.340133003723277	28.760977914104558	22.166170070491653
15	21.400289499344876	25.84427999406319	31.256513336280463	21.498917170311476
16	23.499399137247266	24.38672899254547	29.773587254368422	22.340284615838836
17	23.36406537514423	24.89614570083897	27.626600106288073	24.113188817728723
18	24.342362499780563	24.63521327036909	27.93892106434897	23.08350316550138
19	24.934128525580157	27.179424161225917	26.16266543674663	21.723781876447298
20	26.173198488985776	25.818585730267685	25.71469153318151	22.29352424756503
21	25.5504716732711	27.231291463918712	25.683571151565832	21.53466571124435
22	23.957427317949755	24.87108980384584	25.417053011575184	25.754429866629213
23	23.613826386572594	26.94865456216815	25.897583622061322	23.53993542919794
24	23.116219464122988	25.761771084856505	27.802629752042375	23.319379698978135
25	23.51456034880362	26.771826957910882	25.588135314611097	24.125477378674397
26	22.576959107818556	26.52445982199142	26.573454474073532	24.325126596116494
27	22.37220295595748	30.49270745724139	24.251395230442434	22.883694356358692
28	22.378586623981207	26.74389841030707	27.793533025108562	23.08398194060316
29	23.9845579070506	26.276933094371362	25.82385225638726	23.91465674219078
30	25.380027737037565	25.985837832489363	25.7876249403526	22.846509490120475
31	26.507223918327348	26.908118270217475	23.211336117676538	23.373321693778635
32	26.133460155538074	27.201128632506595	24.29352743939904	22.37188377255629
33	24.993337046500233	26.904288069403236	23.63409453254793	24.4682803515486
34	23.97019465399721	26.21118131372696	25.989987216704787	23.828636815571045
35	25.61510631201135	25.842684077057253	24.604571663855193	23.9376379470762
36	25.956153776179026	27.876361117716435	24.82576576087737	21.34171934522717
37	24.095314547262284	29.03962502334029	25.048874958306666	21.816185471090762
38	23.298473186200425	29.134582085193237	25.61079733609533	21.956147392511
39	25.44035339986179	26.31379877720839	24.451204039585125	23.79464378334469
40	24.73400053303628	25.47610194079467	27.31475792332895	22.475139602840095
41	22.696174108161678	26.260175965809076	26.14319524927426	24.90045467675499
42	22.805973198169802	26.28555104620339	27.006745941184075	23.901729814442728
43	23.229050796442383	24.564673738706894	27.71166248270425	24.494612982146478
44	23.198887965030266	25.644949960022277	26.536110016134725	24.620052058812732
45	24.961897481483373	24.74644868568255	25.45471665291518	24.836937179918895
46	24.8227335185661	25.882582002205556	25.14159773635132	24.153086742877022
47	22.98375835263063	25.890082812133436	27.83039870794559	23.29576012729034
48	22.67431004518041	25.670325040416596	27.85497582983695	23.800389084566046
49	23.18659940408459	25.746450281599554	27.546485072590286	23.520465241725567
50	22.746764677249725	25.156439764506487	27.989990408538794	24.106805149704993
51	22.63201824452321	24.622924709423412	26.590690377737598	26.15436666831578
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1384.0
1	1713.0
2	2042.0
3	2066.0
4	2090.0
5	1590.0
6	1090.0
7	1116.5
8	1143.0
9	1137.0
10	1131.0
11	1178.5
12	1226.0
13	1235.0
14	1244.0
15	1338.5
16	1433.0
17	1388.5
18	1344.0
19	1329.0
20	1314.0
21	1511.5
22	1709.0
23	2106.0
24	2503.0
25	2914.0
26	4000.0
27	4675.0
28	6197.0
29	7719.0
30	9593.0
31	11467.0
32	11701.5
33	11936.0
34	13218.0
35	14500.0
36	14993.0
37	15486.0
38	16834.0
39	18182.0
40	20641.5
41	23101.0
42	26203.0
43	29305.0
44	33116.5
45	36928.0
46	42600.5
47	48273.0
48	52405.0
49	56537.0
50	54829.0
51	53121.0
52	49099.0
53	45077.0
54	41596.5
55	38116.0
56	36454.0
57	34792.0
58	32952.0
59	31112.0
60	29955.0
61	28798.0
62	26686.0
63	24574.0
64	22255.5
65	19937.0
66	17476.5
67	15016.0
68	13035.0
69	11054.0
70	9846.0
71	8638.0
72	7329.5
73	6021.0
74	4867.5
75	3145.5
76	2577.0
77	1980.5
78	1384.0
79	1130.5
80	877.0
81	642.5
82	408.0
83	284.5
84	161.0
85	108.0
86	55.0
87	48.5
88	42.0
89	29.0
90	16.0
91	13.0
92	10.0
93	7.5
94	5.0
95	4.0
96	3.0
97	3.0
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	626599.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.513316859500897
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.96151497400089	23.93799290503412
2	8.831386121279191	5.566125382969394
3	3.2813304224592352	3.1021681597103345
4	1.718403722401663	2.166104039863577
5	1.0834544133552195	1.7071621115443845
6	0.7614374588122843	1.4397251944946805
7	0.6364119976377014	1.403881705432137
8	0.5271729130861347	1.329037363986359
9	0.435473990942013	1.2350906874564371
>10	6.277215272085959	45.76039357706941
>50	0.4400850214835989	8.655516134744923
>100	0.041101334580439644	2.5838446070416534
>500	0.004511122088097033	0.9491577358915084
>1k	5.012357875663371E-4	0.1638003947611074
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCTGC	1037	0.16549659351515084	TruSeq Adapter, Index 13 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	952	0.15193129896472862	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	708	0.11299092401998727	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	701	0.11187378211583485	No Hit
CTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCTGCT	657	0.10485174728973394	TruSeq Adapter, Index 16 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCTGC	645	0.1029366468826155	TruSeq Adapter, Index 13 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.191834011864047E-4	0.0	0.0	0.11634234973244452	0.0
2	3.191834011864047E-4	0.0	0.0	0.5272909787599406	0.0
3	3.191834011864047E-4	0.0	0.0	0.7486446674827122	0.0
4	3.191834011864047E-4	0.0	0.0	1.045006455484289	0.0
5	3.191834011864047E-4	0.0	0.0	1.8539767857912317	0.0
6	3.191834011864047E-4	0.0	0.0	2.5826724906997938	0.0
7	3.191834011864047E-4	0.0	0.0	3.195823804378877	0.0
8	3.191834011864047E-4	0.0	0.0	4.299719597382058	0.0
9	3.191834011864047E-4	0.0	0.0	4.795092236023358	0.0
10	3.191834011864047E-4	0.0	0.0	5.835310940489851	0.0
11	3.191834011864047E-4	0.0	0.0	6.878242703866428	0.0
12	3.191834011864047E-4	0.0	0.0	7.814567211246747	0.0
13	3.191834011864047E-4	0.0	0.0	8.135027346037896	0.0
14	4.7877510177960706E-4	0.0	0.0	8.262860298213052	0.0
15	4.7877510177960706E-4	0.0	0.0	8.497619689785653	0.0
16	4.7877510177960706E-4	0.0	0.0	8.975756424762887	0.0
17	4.7877510177960706E-4	0.0	0.0	9.53512533534206	0.0
18	4.7877510177960706E-4	0.0	0.0	10.199186401510376	0.0
19	4.7877510177960706E-4	0.0	0.0	10.523955512217542	0.0
20	4.7877510177960706E-4	0.0	0.0	10.846969114218185	0.0
21	4.7877510177960706E-4	0.0	0.0	11.211795741774244	0.0
22	4.7877510177960706E-4	0.0	0.0	11.602635816526996	0.0
23	4.7877510177960706E-4	0.0	0.0	11.989805282166106	0.0
24	4.7877510177960706E-4	0.0	0.0	12.296221347305055	0.0
25	4.7877510177960706E-4	0.0	0.0	12.55364276036189	0.0
26	4.7877510177960706E-4	0.0	0.0	12.814415599131182	0.0
27	4.7877510177960706E-4	0.0	0.0	13.078380271912339	0.0
28	4.7877510177960706E-4	0.0	0.0	13.338833927280445	0.0
29	4.7877510177960706E-4	0.0	0.0	13.632801839773125	0.0
30	4.7877510177960706E-4	0.0	0.0	13.951985240959528	0.0
31	4.7877510177960706E-4	0.0	0.0	14.244197644745682	0.0
32	4.7877510177960706E-4	0.0	0.0	14.546304733968615	0.0
33	4.7877510177960706E-4	0.0	0.0	14.835804078844683	0.0
34	7.979585029660118E-4	0.0	0.0	15.136315251061683	0.0
35	7.979585029660118E-4	0.0	0.0	15.460924770068257	0.0
36	7.979585029660118E-4	0.0	0.0	15.770692260919663	0.0
37	7.979585029660118E-4	0.0	0.0	16.063702623208783	0.0
38	7.979585029660118E-4	0.0	0.0	16.364213795425783	0.0
39	7.979585029660118E-4	0.0	0.0	16.66967231036117	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAAATT	30	2.1639153E-6	45.000004	43
TAACGCG	30	2.1639153E-6	45.000004	26
CTAACGC	30	2.1639153E-6	45.000004	25
GAATGCG	20	7.0308277E-4	45.0	1
ACTCTAC	20	7.0308277E-4	45.0	11
TACGATA	20	7.0308277E-4	45.0	34
ACGTGTA	20	7.0308277E-4	45.0	22
TACCTTA	20	7.0308277E-4	45.0	14
CGATCAA	20	7.0308277E-4	45.0	45
TCGACGT	20	7.0308277E-4	45.0	25
GCGTAAT	20	7.0308277E-4	45.0	8
TATCACC	25	3.8887683E-5	44.999996	18
TAGACCG	25	3.8887683E-5	44.999996	13
TAATGCG	25	3.8887683E-5	44.999996	1
CGAATAT	105	0.0	42.857147	14
TATCGAG	55	6.002665E-11	40.909092	1
TCGCACA	40	3.4548611E-7	39.375	34
CGCGATT	40	3.4548611E-7	39.375	22
TTATCCG	40	3.4548611E-7	39.375	1
CCGTATG	40	3.4548611E-7	39.375	1
>>END_MODULE