SRR3553997_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3553997_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	602371
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGC	1382	0.22942671542952767	TruSeq Adapter, Index 19 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGC	1251	0.20767932055162017	TruSeq Adapter, Index 19 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1103	0.18310974465902244	No Hit
CTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGCT	1053	0.17480921226287455	TruSeq Adapter, Index 16 (95% over 23bp)
GCTAAAGGTGTAATTTGAAATGGCCTTCGGGTAAATGCAAGATACTTAACT	865	0.14359921045335847	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	774	0.12849224149236932	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	684	0.11355128317930312	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGCAT	30	2.1637734E-6	45.000004	16
GTCACTC	30	2.1637734E-6	45.000004	42
TTAGGCA	20	7.0306094E-4	45.0	14
TCCATAC	20	7.0306094E-4	45.0	44
CAACGTC	20	7.0306094E-4	45.0	24
CGCGATC	20	7.0306094E-4	45.0	28
CGCGATA	20	7.0306094E-4	45.0	23
ACCTATA	20	7.0306094E-4	45.0	16
ATGACCG	20	7.0306094E-4	45.0	21
TCGTAAG	20	7.0306094E-4	45.0	1
TATAGCG	40	6.8066583E-9	45.0	1
CGATCCA	20	7.0306094E-4	45.0	37
TGTCGTT	20	7.0306094E-4	45.0	24
TAATGCG	20	7.0306094E-4	45.0	1
CCGTTAG	20	7.0306094E-4	45.0	1
TGCGTTA	20	7.0306094E-4	45.0	22
ACGAAAT	20	7.0306094E-4	45.0	42
CGTGAAA	25	3.8885883E-5	44.999996	3
TTGTCGT	25	3.8885883E-5	44.999996	41
TGTATTC	25	3.8885883E-5	44.999996	20