##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3553997_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	602371
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.748649918405768	31.0	31.0	34.0	30.0	34.0
2	31.865378645386315	33.0	31.0	34.0	30.0	34.0
3	31.88571162954392	33.0	31.0	34.0	30.0	34.0
4	35.54581976887997	37.0	35.0	37.0	33.0	37.0
5	35.38123681252915	37.0	35.0	37.0	33.0	37.0
6	35.326584447126436	37.0	35.0	37.0	32.0	37.0
7	35.65904899140231	37.0	35.0	37.0	35.0	37.0
8	35.52417862081674	37.0	35.0	37.0	33.0	37.0
9	37.25681017180442	39.0	37.0	39.0	34.0	39.0
10	36.67907817607421	39.0	37.0	39.0	32.0	39.0
11	36.607228767653154	39.0	35.0	39.0	32.0	39.0
12	36.49331890147434	38.0	35.0	39.0	32.0	39.0
13	36.34382631301972	38.0	35.0	39.0	32.0	39.0
14	37.416107349125376	39.0	36.0	41.0	32.0	41.0
15	37.55725126209595	39.0	36.0	41.0	32.0	41.0
16	37.61152346311492	39.0	36.0	41.0	33.0	41.0
17	37.55621867586587	39.0	36.0	41.0	32.0	41.0
18	37.55947082445868	39.0	36.0	41.0	33.0	41.0
19	37.494027766940974	39.0	36.0	41.0	32.0	41.0
20	37.43619795773701	39.0	36.0	41.0	32.0	41.0
21	37.30653202096382	39.0	35.0	41.0	32.0	41.0
22	37.29404470002706	39.0	35.0	41.0	32.0	41.0
23	36.811484948644605	39.0	35.0	41.0	31.0	41.0
24	36.9224796678459	39.0	35.0	41.0	31.0	41.0
25	36.93551150370785	39.0	35.0	41.0	31.0	41.0
26	36.93634985747986	39.0	35.0	41.0	31.0	41.0
27	34.56862963190459	39.0	35.0	40.0	24.0	41.0
28	35.44413326670772	38.0	35.0	40.0	23.0	41.0
29	36.14589347760765	38.0	35.0	40.0	29.0	41.0
30	36.420491690337016	38.0	35.0	40.0	30.0	41.0
31	36.51412003565909	38.0	35.0	40.0	30.0	41.0
32	36.329811694122064	38.0	35.0	40.0	30.0	41.0
33	36.397404589530375	39.0	35.0	40.0	30.0	41.0
34	36.381965599273535	39.0	35.0	40.0	30.0	41.0
35	36.3300723308393	38.0	35.0	40.0	30.0	41.0
36	36.23008245748883	38.0	35.0	40.0	30.0	41.0
37	36.17016755454695	38.0	35.0	40.0	30.0	41.0
38	36.09612016514739	38.0	35.0	40.0	30.0	41.0
39	36.00820756643331	38.0	35.0	40.0	29.0	41.0
40	35.8702311366251	38.0	35.0	40.0	29.0	41.0
41	35.76062426644045	38.0	34.0	40.0	29.0	41.0
42	35.696330002606366	38.0	34.0	40.0	29.0	41.0
43	35.625813659688134	38.0	34.0	40.0	28.0	41.0
44	35.50215896847624	38.0	34.0	40.0	28.0	41.0
45	35.438719659478956	38.0	34.0	40.0	28.0	41.0
46	35.32209717931308	38.0	34.0	40.0	27.0	41.0
47	35.21550340238823	37.0	34.0	40.0	27.0	41.0
48	35.13569378339927	37.0	34.0	40.0	27.0	41.0
49	35.07884343701805	37.0	34.0	40.0	27.0	41.0
50	35.00039012502262	37.0	34.0	40.0	27.0	41.0
51	33.42836391526153	35.0	31.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	6.0
10	6.0
11	6.0
12	3.0
13	10.0
14	17.0
15	48.0
16	89.0
17	146.0
18	287.0
19	496.0
20	892.0
21	1251.0
22	1917.0
23	2552.0
24	3557.0
25	4710.0
26	5815.0
27	7351.0
28	9180.0
29	11141.0
30	14014.0
31	18000.0
32	23145.0
33	30888.0
34	46407.0
35	56181.0
36	56883.0
37	76196.0
38	105749.0
39	125398.0
40	27.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.96073350144678	18.040377109787823	23.56321934488878	15.435670043876614
2	29.28129010194714	25.723681916958153	26.05238299984561	18.942644981249096
3	31.421997406913682	25.18248720472931	27.3437798300383	16.05173555831871
4	27.663350327289994	27.41118015309502	25.5352598315656	19.390209688049392
5	23.939399473082204	31.071216907852467	24.717989411840875	20.271394207224454
6	24.11221655757	36.93205682212457	24.019084584085224	14.936642036220201
7	81.37924966507352	6.2916375456321765	7.673012146999109	4.656100642295197
8	82.30824525085039	5.5044150531815115	6.835322417579863	5.352017278388236
9	75.94837732892188	7.783575238515798	10.148728939474177	6.119318493088147
10	39.93784561341764	25.59834387777632	17.463988140199312	16.999822368606722
11	30.460297723495984	25.960579111544213	23.086104742758202	20.4930184222016
12	26.741327188725883	22.55686279717981	30.131762651256448	20.57004736283785
13	23.545456205561024	24.854948196377315	30.958661688560706	20.640933909500955
14	20.44968964309371	28.54951516590274	28.462691597039036	22.538103593964518
15	19.627272893283376	26.018516827669323	33.843760738813785	20.51044954023351
16	21.76316588946015	24.373683328048664	31.585517895117793	22.277632887373397
17	22.338226773865273	23.849753723203808	27.603918515333575	26.208100987597344
18	23.295278159141127	24.55480094493261	29.302041432937507	22.847879462988757
19	23.483866255181606	27.756316290126847	25.978010229576125	22.78180722511542
20	24.826892396878335	25.8765777236952	27.110700880354464	22.185828999072
21	24.417842160396166	26.8072334159513	27.044960663776973	21.72996375987556
22	22.57695008557849	25.135174170071267	25.644826859194747	26.643048885155494
23	20.97361924793856	28.220482061719437	26.292600407390132	24.51329828295187
24	21.467832946805206	25.24872545325057	29.154291956286077	24.129149643658142
25	22.529637050920446	26.01951289155686	26.37527371005576	25.075576347466928
26	21.418527784372092	26.457947012721394	27.28600812456111	24.837517078345407
27	21.218484953624923	30.752144442544544	25.318117904082367	22.71125269974816
28	20.312398837261423	26.14534896268247	29.319472550969415	24.222779649086693
29	22.419073959403757	24.80746915107135	27.69074208419728	25.082714805327615
30	23.220075335632025	25.14413874505911	27.000303799485696	24.635482119823166
31	23.960814846664267	26.519038931157045	24.012278147520384	25.507868074658308
32	23.427256624239877	25.59004334538017	27.028691620280526	23.954008410099423
33	22.733498126569838	24.6834591970729	26.564857869983783	26.01818480637348
34	20.78536317319393	24.723135741926487	27.66882867867145	26.822672406208138
35	22.617788704967538	23.61484865639282	28.14162700395603	25.62573563468361
36	23.126113308907634	25.187633534814925	27.347598074940528	24.338655081336917
37	22.852361750482675	25.101474008542908	28.307305630583144	23.73885861039127
38	21.383001505716575	25.911273949111095	27.65770596526061	25.048018579911712
39	21.579226091561512	24.04083197896313	28.179809452978315	26.20013247649704
40	22.992972769273422	23.484198276477454	29.105152804500882	24.417676149748242
41	21.01561994186307	23.42841869877534	29.17570732986814	26.38025402949345
42	21.34332496086299	23.658343446148635	29.405134045297665	25.59319754769071
43	22.385705819171243	22.4215641191226	29.847220400716502	25.345509660989656
44	21.555652579556455	23.43240295432549	28.165366526609013	26.846577939509043
45	21.88999802447329	23.54695030139233	27.776901610469295	26.786150063665083
46	22.747110999699522	24.11719687700769	27.7906804942469	25.34501162904589
47	21.65592301090192	23.121797032061636	30.11134334156193	25.110936615474515
48	21.57906008091359	23.47324157371454	29.58044128950431	25.367257055867565
49	21.726975568212943	22.656303175285665	30.831995564195484	24.784725692305905
50	21.247038785067673	23.097725488112808	30.15135190771136	25.503883819108157
51	20.5255565091945	22.93055276565439	28.171508920582166	28.372381804568946
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1756.0
1	1988.5
2	2221.0
3	1894.5
4	1568.0
5	1273.5
6	979.0
7	994.0
8	1009.0
9	1080.5
10	1152.0
11	1214.0
12	1276.0
13	1344.0
14	1412.0
15	1242.0
16	1072.0
17	1145.5
18	1219.0
19	1316.5
20	1414.0
21	1690.5
22	1967.0
23	2081.0
24	2195.0
25	2887.0
26	3926.5
27	4274.0
28	4981.5
29	5689.0
30	6912.0
31	8135.0
32	8990.0
33	9845.0
34	11645.5
35	13446.0
36	14567.5
37	15689.0
38	16936.0
39	18183.0
40	20497.5
41	22812.0
42	27598.5
43	32385.0
44	36131.0
45	39877.0
46	44435.0
47	48993.0
48	54715.5
49	60438.0
50	58602.5
51	56767.0
52	51523.5
53	46280.0
54	41021.0
55	35762.0
56	33451.0
57	31140.0
58	29693.5
59	28247.0
60	26964.0
61	25681.0
62	23134.0
63	20587.0
64	18418.0
65	16249.0
66	14292.5
67	12336.0
68	10465.5
69	8595.0
70	7695.5
71	6796.0
72	5523.0
73	4250.0
74	3673.5
75	2385.5
76	1674.0
77	1515.5
78	1357.0
79	970.5
80	584.0
81	395.5
82	207.0
83	144.5
84	82.0
85	62.0
86	42.0
87	30.5
88	19.0
89	11.0
90	3.0
91	3.0
92	3.0
93	3.5
94	4.0
95	6.0
96	8.0
97	12.0
98	16.0
99	8.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	602371.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.20928171985909
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.48408137878553	23.55803961115287
2	9.918421460766895	6.190936191707407
3	3.7252060923270123	3.487830191999074
4	1.837489843571958	2.293869527416682
5	1.1276978985235635	1.7597320704957484
6	0.7334137478869196	1.373358976501235
7	0.5613753248760456	1.2264084465243748
8	0.4701024466652772	1.1737247756137335
9	0.3790714915913808	1.0647494075738342
>10	4.8591756622296955	36.65737794723171
>50	0.8432072389421277	16.799135664024174
>100	0.056002485794399516	3.0640866292173383
>500	0.0026416266884150713	0.5611089059477266
>1k	0.002113301350732057	0.7896416545940823
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGC	1382	0.22942671542952767	TruSeq Adapter, Index 19 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGC	1251	0.20767932055162017	TruSeq Adapter, Index 19 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1103	0.18310974465902244	No Hit
CTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGCT	1053	0.17480921226287455	TruSeq Adapter, Index 16 (95% over 23bp)
GCTAAAGGTGTAATTTGAAATGGCCTTCGGGTAAATGCAAGATACTTAACT	865	0.14359921045335847	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	774	0.12849224149236932	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	684	0.11355128317930312	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.660106479229578E-4	0.0	0.0	0.19058022381555553	0.0
2	1.660106479229578E-4	0.0	0.0	0.8516346238447734	0.0
3	1.660106479229578E-4	0.0	0.0	1.1461375132601006	0.0
4	1.660106479229578E-4	0.0	0.0	1.526301897003674	0.0
5	1.660106479229578E-4	0.0	0.0	2.5972365867546743	0.0
6	1.660106479229578E-4	0.0	0.0	3.601933027984415	0.0
7	1.660106479229578E-4	0.0	0.0	4.415385202806908	0.0
8	1.660106479229578E-4	0.0	0.0	5.831788050885584	0.0
9	1.660106479229578E-4	0.0	0.0	6.489522237956343	0.0
10	1.660106479229578E-4	0.0	0.0	7.741076512647521	0.0
11	1.660106479229578E-4	0.0	0.0	9.066173504368571	0.0
12	1.660106479229578E-4	0.0	0.0	10.194381867652991	0.0
13	1.660106479229578E-4	0.0	0.0	10.643772691580438	0.0
14	1.660106479229578E-4	0.0	0.0	10.811111424686779	0.0
15	1.660106479229578E-4	0.0	0.0	11.103456175679108	0.0
16	1.660106479229578E-4	0.0	0.0	11.79223435391146	0.0
17	1.660106479229578E-4	0.0	0.0	12.516538810799325	0.0
18	1.660106479229578E-4	0.0	0.0	13.494673548361392	0.0
19	1.660106479229578E-4	0.0	0.0	13.911194264000093	0.0
20	1.660106479229578E-4	0.0	0.0	14.30779370188804	0.0
21	1.660106479229578E-4	0.0	0.0	14.75353229156118	0.0
22	1.660106479229578E-4	0.0	0.0	15.220188222872615	0.0
23	1.660106479229578E-4	0.0	0.0	15.705603357399344	0.0
24	1.660106479229578E-4	0.0	0.0	16.10436093371029	0.0
25	1.660106479229578E-4	0.0	0.0	16.435220155020744	0.0
26	0.0036522342543050712	0.0	0.0	16.756284748103745	0.0
27	0.0036522342543050712	0.0	0.0	17.08315971386405	0.0
28	0.0036522342543050712	0.0	0.0	17.440746649490098	0.0
29	0.0036522342543050712	0.0	0.0	17.80397794714553	0.0
30	0.0036522342543050712	0.0	0.0	18.217842492417464	0.0
31	0.0036522342543050712	0.0	0.0	18.59535070579427	0.0
32	0.0036522342543050712	0.0	0.0	18.986471792300758	0.0
33	0.0036522342543050712	0.0	0.0	19.34970308995619	0.0
34	0.0036522342543050712	0.0	0.0	19.714926515386697	0.0
35	0.0036522342543050712	0.0	0.0	20.11418212364141	0.0
36	0.0036522342543050712	0.0	0.0	20.475089272225922	0.0
37	0.003818244902228029	0.0	0.0	20.826035781935055	0.0
38	0.003818244902228029	0.0	0.0	21.197235590690788	0.0
39	0.003818244902228029	0.0	0.0	21.568103378150674	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGCAT	30	2.1637734E-6	45.000004	16
GTCACTC	30	2.1637734E-6	45.000004	42
TTAGGCA	20	7.0306094E-4	45.0	14
TCCATAC	20	7.0306094E-4	45.0	44
CAACGTC	20	7.0306094E-4	45.0	24
CGCGATC	20	7.0306094E-4	45.0	28
CGCGATA	20	7.0306094E-4	45.0	23
ACCTATA	20	7.0306094E-4	45.0	16
ATGACCG	20	7.0306094E-4	45.0	21
TCGTAAG	20	7.0306094E-4	45.0	1
TATAGCG	40	6.8066583E-9	45.0	1
CGATCCA	20	7.0306094E-4	45.0	37
TGTCGTT	20	7.0306094E-4	45.0	24
TAATGCG	20	7.0306094E-4	45.0	1
CCGTTAG	20	7.0306094E-4	45.0	1
TGCGTTA	20	7.0306094E-4	45.0	22
ACGAAAT	20	7.0306094E-4	45.0	42
CGTGAAA	25	3.8885883E-5	44.999996	3
TTGTCGT	25	3.8885883E-5	44.999996	41
TGTATTC	25	3.8885883E-5	44.999996	20
>>END_MODULE