##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3553996_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 457599 Sequences flagged as poor quality 0 Sequence length 51 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.674341508613438 31.0 31.0 34.0 30.0 34.0 2 31.791422184051974 31.0 31.0 34.0 30.0 34.0 3 31.81511978828625 31.0 31.0 34.0 30.0 34.0 4 35.47142148474975 37.0 35.0 37.0 33.0 37.0 5 35.32003347909414 37.0 35.0 37.0 33.0 37.0 6 35.25675755410305 37.0 35.0 37.0 32.0 37.0 7 35.5821538071543 37.0 35.0 37.0 35.0 37.0 8 35.42256866820076 37.0 35.0 37.0 33.0 37.0 9 37.21184923918103 39.0 37.0 39.0 34.0 39.0 10 36.65526804035848 39.0 35.0 39.0 32.0 39.0 11 36.55412927038739 38.0 35.0 39.0 32.0 39.0 12 36.4361788378034 38.0 35.0 39.0 32.0 39.0 13 36.234148238960316 38.0 35.0 39.0 32.0 39.0 14 37.264917536970145 39.0 36.0 41.0 32.0 41.0 15 37.426189742547514 39.0 36.0 41.0 32.0 41.0 16 37.49981315518609 39.0 36.0 41.0 32.0 41.0 17 37.44925797477704 39.0 36.0 41.0 32.0 41.0 18 37.44254248807362 39.0 36.0 41.0 32.0 41.0 19 37.36858253623806 39.0 36.0 41.0 32.0 41.0 20 37.274908817545494 39.0 35.0 41.0 32.0 41.0 21 37.16510307059237 39.0 35.0 40.0 32.0 41.0 22 37.16871758898075 39.0 35.0 40.0 32.0 41.0 23 36.700276879975696 39.0 35.0 40.0 31.0 41.0 24 36.78496237972548 39.0 35.0 40.0 31.0 41.0 25 36.7904366049751 39.0 35.0 40.0 31.0 41.0 26 36.80502361237677 39.0 35.0 40.0 31.0 41.0 27 34.43418582645504 38.0 35.0 40.0 24.0 41.0 28 35.3179049779392 38.0 34.0 40.0 23.0 41.0 29 35.99816870229174 38.0 34.0 40.0 28.0 41.0 30 36.285632180140254 38.0 35.0 40.0 30.0 41.0 31 36.40026529778255 38.0 35.0 40.0 30.0 41.0 32 36.186641579199254 38.0 35.0 40.0 30.0 41.0 33 36.20674870355923 38.0 35.0 40.0 30.0 41.0 34 36.192327780436585 38.0 35.0 40.0 30.0 41.0 35 36.145854776780546 38.0 35.0 40.0 30.0 41.0 36 36.01620414380276 38.0 35.0 40.0 30.0 41.0 37 35.98021630292024 38.0 35.0 40.0 30.0 41.0 38 35.88918682077539 38.0 35.0 40.0 29.0 41.0 39 35.786787121475356 38.0 35.0 40.0 29.0 41.0 40 35.63721074565285 38.0 34.0 40.0 28.0 41.0 41 35.537085963911636 38.0 34.0 40.0 28.0 41.0 42 35.49196567300191 38.0 34.0 40.0 28.0 41.0 43 35.399793268779 38.0 34.0 40.0 28.0 41.0 44 35.285273787748665 38.0 34.0 40.0 27.0 41.0 45 35.20651268905745 37.0 34.0 40.0 27.0 41.0 46 35.03225750056272 37.0 34.0 40.0 27.0 41.0 47 34.921468359852184 37.0 34.0 40.0 26.0 41.0 48 34.864105909322355 37.0 34.0 40.0 26.0 41.0 49 34.82989254784211 37.0 34.0 40.0 26.0 41.0 50 34.697639199386366 37.0 33.0 40.0 26.0 41.0 51 33.078385223743936 35.0 31.0 39.0 22.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 3.0 11 3.0 12 4.0 13 6.0 14 11.0 15 30.0 16 57.0 17 104.0 18 206.0 19 417.0 20 729.0 21 1082.0 22 1663.0 23 2232.0 24 2925.0 25 3850.0 26 4688.0 27 5773.0 28 7121.0 29 8932.0 30 11189.0 31 14313.0 32 18224.0 33 24592.0 34 36711.0 35 45455.0 36 43549.0 37 57760.0 38 79941.0 39 86013.0 40 16.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.11657149600414 18.813196707160635 22.921160229808194 15.149071567027026 2 28.0822292006757 27.72055882989255 26.739350391937045 17.457861577494707 3 31.044211198013983 26.343589037563458 26.315179884571428 16.297019879851135 4 27.90456272850247 27.266886509804433 25.973614452828787 18.85493630886431 5 24.25180124956567 30.949805397302004 24.27452857195929 20.523864781173035 6 23.293757197895975 37.774339541825924 24.431215977307644 14.500687282970462 7 78.13500466565705 7.394465459933261 9.111908024274529 5.358621850135162 8 79.15620444974749 6.9744470595434 7.848574843913557 6.020773646795557 9 72.80916260743578 8.435114587225934 10.93293473106366 7.822788074274638 10 41.228674013710695 23.97601393359688 17.50047530698275 17.29483674570967 11 30.222312548759938 26.69433281104198 22.75726127023879 20.32609336995929 12 26.662208614966374 22.071726555346494 30.021918754193084 21.244146075494047 13 23.386633274985304 25.794418257032902 32.0807082183309 18.738240249650893 14 19.88902947777421 30.505748482841966 28.228427072611613 21.376794966772216 15 18.212452387352247 26.45110675504099 34.8545342100835 20.481906647523267 16 19.803802018798116 24.26229078297811 32.276949905921995 23.656957292301776 17 20.11215059473469 24.260323995463278 27.714002871509773 27.913522538292263 18 21.830904350752515 24.654337094268126 30.2089821000483 23.305776454931067 19 22.671378215424422 27.490007626764918 26.74634341421201 23.092270743598654 20 24.50486124314083 25.04550927777377 27.439745279163635 23.009884199921764 21 22.451098013763144 27.834632505752854 27.92007849667504 21.794190983808967 22 20.734966641098428 25.634015808600978 25.789173490326682 27.84184405997391 23 20.061669715187314 28.42532435604099 26.286115135741117 25.22689079303058 24 22.305337205719418 25.192581277494053 28.55709911953479 23.944982397251742 25 20.40804284974399 27.708321040911365 27.315182069890888 24.56845403945376 26 19.889685073612487 28.064746644988297 27.68515665462556 24.360411626773658 27 20.048776330367854 32.254878179366656 24.719896678095886 22.976448812169608 28 19.277795624553377 27.926634455057815 29.452205970729832 23.34336394965898 29 21.58767829475152 27.367411205007002 26.43253153962312 24.612378960618358 30 21.82216307290881 28.10102294803966 26.247216449336648 23.82959752971488 31 23.253328787868853 27.71968470210818 24.26382050660076 24.76316600342221 32 23.98956291425462 28.913961787503904 24.285236637317826 22.811238660923646 33 22.35035478661448 27.66308492807021 23.600576050209902 26.385984235105408 34 20.34357592564669 27.791581712372622 27.14232330053169 24.722519061448995 35 22.50420127666363 26.15171798889421 25.295946887995825 26.04813384644634 36 23.762945286156658 27.47492892248453 25.813649068289045 22.94847672306976 37 23.251799064246207 27.05228813874156 27.52103916311006 22.174873633902173 38 23.168756924731042 28.069772879748427 25.54878834962489 23.21268184589564 39 23.74349594295442 25.62702278632602 27.389045867670163 23.240435403049396 40 24.470988791496485 24.805779732910256 27.885987513084597 22.837243962508662 41 20.554022189733807 25.126803161720197 26.54813493910607 27.771039709439926 42 22.535014281062676 25.25224049877731 26.868284240131647 25.344460980028366 43 22.468799101396637 22.998083474832768 28.677073157939592 25.856044265831002 44 21.943666834936266 24.517536096014194 28.510551815017077 25.02824525403246 45 23.224264039038548 24.75027261860275 27.13139670322706 24.89406663913164 46 24.17618919621765 25.80359659876879 26.901064032045525 23.119150172968038 47 21.508569730266018 25.0007102288248 29.534373982460625 23.956346058448556 48 22.050528956575516 24.499397944488514 27.628338348641496 25.82173475029447 49 22.653458595844832 23.14012923979292 28.90347225409147 25.302939910270783 50 22.162198781028806 23.414605364085148 29.070430660906165 25.35276519397988 51 21.201095282113815 24.33112834599726 26.324358226307314 28.143418145581613 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2485.0 1 2623.0 2 2761.0 3 2296.0 4 1831.0 5 1376.0 6 921.0 7 962.0 8 1003.0 9 1031.0 10 1059.0 11 1088.0 12 1117.0 13 1105.5 14 1094.0 15 1186.5 16 1279.0 17 1229.5 18 1180.0 19 1187.0 20 1194.0 21 1444.5 22 1695.0 23 1802.5 24 1910.0 25 2298.5 26 3054.0 27 3421.0 28 3980.0 29 4539.0 30 5434.5 31 6330.0 32 7037.5 33 7745.0 34 8087.5 35 8430.0 36 8923.0 37 9416.0 38 11182.5 39 12949.0 40 14763.0 41 16577.0 42 20140.0 43 23703.0 44 28703.0 45 33703.0 46 39331.5 47 44960.0 48 46586.5 49 48213.0 50 46417.0 51 44621.0 52 39270.5 53 33920.0 54 29589.5 55 25259.0 56 23733.0 57 22207.0 58 20608.0 59 19009.0 60 17797.0 61 16585.0 62 15169.5 63 13754.0 64 12425.0 65 11096.0 66 9751.5 67 8407.0 68 7371.0 69 6335.0 70 5260.0 71 4185.0 72 3968.0 73 3751.0 74 3186.0 75 2077.0 76 1533.0 77 1267.5 78 1002.0 79 780.0 80 558.0 81 419.0 82 280.0 83 227.0 84 174.0 85 126.0 86 78.0 87 44.0 88 10.0 89 7.5 90 5.0 91 3.0 92 1.0 93 2.0 94 3.0 95 3.0 96 3.0 97 1.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 457599.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.30122603064348 #Duplication Level Percentage of deduplicated Percentage of total 1 76.03229592888763 24.559363764277737 2 10.020524213394943 6.4735043512481205 3 3.742941291054481 3.627047779853379 4 1.9623025807506511 2.5353911680536725 5 1.1419828185305105 1.844372257223267 6 0.7594497305317989 1.471869444289135 7 0.5327511174633788 1.2045959984283745 8 0.4333991530182785 1.1199459202506283 9 0.34052203006188086 0.9899351155298146 >10 4.14686011905223 32.4495707912847 >50 0.8043840111056232 16.864609323911825 >100 0.07251542003289424 3.8986897620421943 >500 0.005371512595029203 1.0802944873571523 >1k 0.004700073520650553 1.880809836249995 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1740 0.3802455862010188 No Hit GCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTCTGC 1558 0.34047277201217657 Illumina Single End Adapter 1 (95% over 21bp) CCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTCTGC 1206 0.2635495269876027 Illumina Single End Adapter 1 (95% over 21bp) GAATGATACCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCG 1057 0.2309882670198143 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1055 0.23055120312762922 No Hit CTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTCTGCT 1039 0.22705469199014858 Illumina Single End Adapter 1 (95% over 22bp) GAATCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTC 1017 0.2222469891761127 No Hit GAATGATACGGCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGC 875 0.1912154528309721 No Hit TCGAATGGGGTTTTATTAACAATGCTTCTGGAAACGAATGCATTTTAAATA 744 0.1625877678928494 No Hit CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 673 0.14707199972027912 No Hit ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC 561 0.12259642175791467 No Hit TCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTCTGC 558 0.12194082591963706 Illumina Single End Adapter 1 (95% over 21bp) CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCC 538 0.11757018699778626 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 529 0.11560339948295342 No Hit GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA 503 0.10992156888454739 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.24781522686893984 0.0 2 0.0 0.0 0.0 1.151881887853776 0.0 3 0.0 0.0 0.0 1.512022535014281 0.0 4 0.0 0.0 0.0 2.0303803111457848 0.0 5 0.0 0.0 0.0 3.6210743467533804 0.0 6 0.0 0.0 0.0 4.792405577809392 0.0 7 0.0 0.0 0.0 5.801367572918647 0.0 8 0.0 0.0 0.0 7.451939361755598 0.0 9 0.0 0.0 0.0 8.160419930987612 0.0 10 0.0 0.0 0.0 9.883325793981193 0.0 11 0.0 0.0 0.0 11.565803246947654 0.0 12 0.0 0.0 0.0 13.119346851719518 0.0 13 0.0 0.0 0.0 13.666113780843052 0.0 14 0.0 0.0 0.0 13.875904449091891 0.0 15 0.0 0.0 0.0 14.277347634063887 0.0 16 0.0 0.0 0.0 15.107769029215536 0.0 17 0.0 0.0 0.0 16.03674833205492 0.0 18 0.0 0.0 0.0 17.28194336089021 0.0 19 0.0 0.0 0.0 17.822591395523155 0.0 20 0.0 0.0 0.0 18.276045183665175 0.0 21 0.0 0.0 0.0 18.82128238916606 0.0 22 0.0 0.0 0.0 19.345103463949876 0.0 23 0.0 0.0 0.0 19.905856437623335 0.0 24 0.0 0.0 0.0 20.36346233274111 0.0 25 0.0 0.0 0.0 20.732999853583596 0.0 26 0.0010926597304626977 0.0 0.0 21.08942545766053 0.0 27 0.0010926597304626977 0.0 0.0 21.4622409576944 0.0 28 0.0010926597304626977 0.0 0.0 21.862154419043748 0.0 29 0.0010926597304626977 0.0 0.0 22.27539832910474 0.0 30 0.0010926597304626977 0.0 0.0 22.70918424209843 0.0 31 0.0010926597304626977 0.0 0.0 23.124176407728164 0.0 32 0.0010926597304626977 0.0 0.0 23.54288361644147 0.0 33 0.0010926597304626977 0.0 0.0 23.95437927093372 0.0 34 0.0010926597304626977 0.0 0.0 24.334187793242556 0.0 35 0.0010926597304626977 0.0 0.0 24.75289500195586 0.0 36 0.0010926597304626977 0.0 0.0 25.130081140911585 0.0 37 0.0010926597304626977 0.0 0.0 25.50835993959777 0.0 38 0.0010926597304626977 0.0 0.0 25.88008277990118 0.0 39 0.0010926597304626977 0.0 0.0 26.259017174425644 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGTTTA 35 1.2097917E-7 45.000004 38 ACTATAG 35 1.2097917E-7 45.000004 26 CTGTCGG 20 7.0288294E-4 45.000004 2 CGAACTA 25 3.887112E-5 45.000004 11 ATTAGTC 40 6.7993824E-9 45.000004 31 CATATAA 25 3.887112E-5 45.000004 34 ATTAGCG 20 7.0288294E-4 45.000004 1 CAACGAC 20 7.0288294E-4 45.000004 12 CGTTGAT 40 6.7993824E-9 45.000004 25 CAACCCG 25 3.887112E-5 45.000004 23 ATTCGGT 20 7.0288294E-4 45.000004 10 CACGATA 20 7.0288294E-4 45.000004 23 TAAGTGC 25 3.887112E-5 45.000004 13 CAATTCG 20 7.0288294E-4 45.000004 20 TAACGCA 50 2.1827873E-11 45.000004 26 TACGCTA 25 3.887112E-5 45.000004 1 CCCGTAG 20 7.0288294E-4 45.000004 20 CCGTAGG 20 7.0288294E-4 45.000004 1 GCCGAAC 25 3.887112E-5 45.000004 9 CGGATCG 20 7.0288294E-4 45.000004 2 >>END_MODULE