FastQCFastQC Report
Sat 18 Jun 2016
SRR3553992_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3553992_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1432019
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27810.19420133392084882No Hit
ATATATATATGTATATATATACATATATATGTATATATATACATATATATG20560.14357351403857072No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20110.1404310976320845No Hit
GCTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCGTCTTCTGC18530.12939772447153286No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATAT207.033868E-445.00000437
CGTTCAT750.042.017
ACACGAC4500.041.526
AACACGT4950.040.45454441
TCGCGAA451.9288564E-840.015
TACGGGT1600.039.3750044
CACGACC4800.038.90625427
ATAACGC1100.038.86363611
TCAAGCG5050.038.76237517
TTAACGC356.2493655E-638.57142614
GACACGA4850.038.04123725
GTTAGCG950.037.8947371
GCTACGA1550.037.74193610
ACGACCA5250.037.71428328
TGCGATA301.1400618E-437.50000444
TTTAGCG1150.037.1739161
TGGTCAA5150.037.1359214
CGTAAGG2850.037.1052632
ACACGTG5450.036.7431242
GCGATAT1350.036.6666649
Produced by FastQC (version 0.11.5)